Papers by Aondohemba S Nege
Journal of the Saudi Society of Agricultural Sciences, Dec 1, 2020
Sargassum duplicatum and Padina tetrastromatica seaweed species collected from oil and a non-oil ... more Sargassum duplicatum and Padina tetrastromatica seaweed species collected from oil and a non-oil extraction site at Madura Island were investigated in this study for selected bioactivities, heavy metals and toxicity effects. The collected seaweeds were evaluated for their phytochemical constituents, total phenolic contents (TPC), antioxidant activities, antidiabetic activities, anticancer activities, toxicities and heavy metals using Folin-Ciocalteus method, the 2,2-diphernyl-1 picrylhydrazyl (DPPH), a-glucosidase enzyme, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Brine Shrimp Lethality Test (BSLT) and atomic absorption spectrophotometer, respectively. The crude extracts (C), normal hexane (N), ethyl acetate (E), and methanol residue fractions (M) were studied. The highest TPC (589.79 ± 7.14 mg GAE/g) was observed in ethyl acetate fraction of P. tetrastromatica from the non-oil extraction site. Meanwhile, the crude extracts and all fractions showed potent antioxidant, antidiabetic, and cytotoxic activities with ethyl acetate fraction of P. tetrastromatica from non-oil extraction site displaying the highest effects (IC 50 25.25 ± 5.15, 249.12 ± 1.77 and 70.56 ± 2.56 mg/mL: antioxidant, antidiabetic, cytotoxic activities respectively). In the brine shrimp assay, the crude extracts and all fractions of both species from the two sites were non-toxic with exception of the hexane fraction of P. tetrastromatica from oil site which was very toxic after 24 h incubation. However, the crude extract of S. duplicatum and ethyl acetate fraction of P. tetrastromatica from oil extraction site were mildly toxic except the hexane fraction of P. tetrastromatica from oil site which was very toxic after 48 h incubation while samples of both species from the non-oil site were nontoxic. In each species, the concentration of Cd from the oil extraction site was higher than those of the non-oil site with the values of P. tetrastromatica leading and a similar observation occurred Cu in P. tetrastromatica but the same Cu was negligible in S. duplicatum as Pb was negligible in both species. According to the findings of this current study, it is safe to conclude that both S. duplicatum and P. tetrastromatica from Madura Island have antioxidant, antidiabetic and cytotoxic activity, we therefore recommend these species from the non-oil extraction site for drug candidates against the various health abnormally they seem to inhibit.
Journal of Pharmacy & Pharmacognosy Research, Jul 1, 2021
La catequina del té como agente antiviral a través de un agonista de la apoptosis y un mecanismo ... more La catequina del té como agente antiviral a través de un agonista de la apoptosis y un mecanismo de triple inhibidor contra la
Jurnal Ilmiah Perikanan dan Kelautan, Mar 21, 2020
There is a need to have a single document that summarises the present day uses of Spirulina. In t... more There is a need to have a single document that summarises the present day uses of Spirulina. In this review, the research trend on the health and other applications of Spirulina microalga was critically evaluated. In terms of the health uses, antioxidant, antibacterial, and immunostimulant effects of Spirulina were emphasized. Other uses of the microalga discussed include the use of Spirulina for human and animal food, bioenergy, pollution and ecotoxicology control, cosmetics, bioplastics and biofertilizers. Literature search revealed that Spirulina polysaccharides, phycocyanin size and content play a role in antioxidant activity and DNA repair. The double bonds and positions of-COOH and-OH in Spirulina phenol content and γ-linolenic fatty acids (γ-LFA) have antimicrobial activity. Some compounds in Spirulina improve immune, increase survival rate and enhance distribution of proteins like hepcidin and TNF-α in animal models. High protein, vitamins, fatty acids (FAs) and glycoproteins in Spirulina are easily digestible due to its lack of cellulose and can improve human and livestock growth. Spirulina produces biodegradable and non-toxic biodiesel and useful co-products. Absorption of heavy metals by chemisorption occurs in Spirulina. Phycocyanin and β-carotene of Spirulina increase skin health, Spirulina also cause high cell proliferation and aids wound healing. Bioplastics produced from Spirulina are biodegradable, non-toxic with high blends. Biofertilizers from Spirulina have little or no residual risks, adds soil Nitrogen through Spirulina Nitrogen fixation ability. In addition, the survey of published works on Spirulina for the past two decades indicates that more research is been carried out in recent years using Spirulina, especially studies involving its health potentials and those concerned with molecular analysis. In conclusion, Spirulina is an exceptional commodity with numerous applications, and probably, some of its compounds causing those effects are yet to be isolated and that is one area for further research.
The study assessed the levels of Escherichia coli, Salmonella, and Vibrio cholerae in seafood (An... more The study assessed the levels of Escherichia coli, Salmonella, and Vibrio cholerae in seafood (Anadaragranosa, Pernaviridis, Anadara antiquata, Penaeus monodon, Litopenaeus vannamei) collected from the main traditional seafood market (Pasar Pabean), Surabaya, Indonesia. The aim of the study was to determine and evaluate the level of bacterial contamination if any, found in shellfish and shrimp sold at Pabean market. The total number of Escherichia coli in green mussel (Pernaviridis) samples was about 21,500 CFU/mL and the lowest was recorded in tiger prawn samples (Penaeus monodon) which was 2,800 CFU/mL. The highest Salmonella concentration was obtained from blood clam (Anadara granosa) samples which contained 470 CFU/mL however it was not present in shrimp samples. The highest concentration of Vibrio cholerae was obtained from the antique ark (Anadara antiquata) samples and was about 120 CFU/mL but was absent in green mussels samples, tiger prawn, and vannamei shrimp. In all, it can be concluded that the concentration of Escherichia coli obtained from all the seafood samples in this research exceeds the threshold for human consumption. All the shellfish samples contained Salmonella but Vibrio cholera was only present in two species including blood clam and antique ark.
This study was aimed at identifying the fish habitat that harbours O. niloticus with higher nutri... more This study was aimed at identifying the fish habitat that harbours O. niloticus with higher nutrients and availing these findings for nutritional guidance. Amino acid and proximate compositions of O. niloticus from Lower Benue River and UAM fish farm were determined monthly for a 3month period using methods of Benitez (1989) and AOAC (2006) respectively. Mean Glutamic acid (12.51±0.64 and 11.85±0.67) and Alanine (5.40±0.23 and 4.50±0.41) varied significantly (p<0.05) between the wild and cultured while other amino acids did not. In October, ash (4.62±0.02 and 3.69±0.02), fat (4.71±0.06 and 3.61±0.02), protein (14.62±0.07 and 9.98±0.02) and moisture (75.67±0.07 and 79.65±0.15) varied significantly between the wild and cultured O. niloticus. Crude protein (20.46±0.01 and 18.75±0.04) and moisture (69.32±0.02 and 73.35±0.20) significantly varied in November between the samples respectively. Only crude protein (18.89±0.04 and 20.31±0.06) varied statistically in December between wild and cultured. Mean crude protein (17.99±1.22 and 16.34±1.86) and mean moisture (72.53±1.16 and 74.94±1.52) differed significantly between the wild and cultured. From these results, O. niloticus wild expressed superiority over the cultured, however, both River Benue at Makurdi and the UAM fish farm harbour nutritious O. niloticus which is good for human consumption; based on our daily amino acid and protein needs.
Journal of Aquaculture and Fish Health, 2020
Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandang... more Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandangin Island. This study aimed to identify Caulerpa sp. based on molecular analysis using certain genetic markers. This research is expected to provide information on the identification of macroalgae from Indonesia waters, especially Mandangin Island, Madura with the use of molecular analysis based on 18SrRNA primers. The two green seaweed samples from the Caulerpa genus in this study were successfully analyzed using 18SrRNA primers. The BLAST results of samples 1and 2 are related to Caulerpa taxifolia 18SrRNA, but in the phylogenetic tree result, Sample 1 was more closely related to Caulerpa sertularioides f. longipes. 18SrRNA primers have been used for molecular identification of green seaweed from Mandangin for the first time and this shows that barcode markers can be used for molecular identification of seaweed, specifically Caulerpa in the waters of Mandangin Island, Indonesia.
Journal of Aquacuture and fish health, 2020
Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandang... more Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandangin Island. This study aimed to identify Caulerpa sp. based on molecular analysis using certain genetic markers. This research is expected to provide information on the identification of macroalgae from Indonesia waters, especially Mandangin Island, Madura with the use of molecular analysis based on 18SrRNA primers. The two green seaweed samples from the Caulerpa genus in this study were successfully analyzed using 18SrRNA primers. The BLAST results of samples 1and 2 are related to Caulerpa taxifolia 18SrRNA, but in the phylogenetic tree result, Sample 1 was more closely related to Caulerpa sertularioides f. longipes. 18SrRNA primers have been used for molecular identification of green seaweed from Mandangin for the first time and this shows that barcode markers can be used for molecular identification of seaweed, specifically Caulerpa in the waters of Mandangin Island, Indonesia.
Research Article, 2020
The study assessed the levels of Escherichia coli, Salmonella, and Vibrio cholerae in seafood (An... more The study assessed the levels of Escherichia coli, Salmonella, and Vibrio cholerae in seafood (Anadaragranosa, Pernaviridis, Anadara antiquata, Penaeus monodon, Litopenaeus vannamei) collected from the main traditional seafood market (Pasar Pabean), Surabaya, Indonesia. The aim of the study was to determine and evaluate the level of bacterial contamination if any, found in shellfish and shrimp sold at Pabean market. The total number of Escherichia coli in green mussel (Pernaviridis) samples was about 21,500 CFU/mL and the lowest was recorded in tiger prawn samples (Penaeus monodon) which was 2,800 CFU/mL. The highest Salmonella concentration was obtained from blood clam (Anadara granosa) samples which contained 470 CFU/mL however it was not present in shrimp samples. The highest concentration of Vibrio cholerae was obtained from the antique ark (Anadara antiquata) samples and was about 120 CFU/mL but was absent in green mussels samples, tiger prawn, and vannamei shrimp. In all, it can be concluded that the concentration of Escherichia coli obtained from all the seafood samples in this research exceeds the threshold for human consumption. All the shellfish samples contained Salmonella but Vibrio cholera was only present in two species including blood clam and antique ark.
Ecology, Environment and Conservation - EM International ..., 2020
The study assessed the levels of Escherichia coli, Salmonella, and Vibrio cholerae in seafood (An... more The study assessed the levels of Escherichia coli, Salmonella, and Vibrio cholerae in seafood (Anadaragranosa, Pernaviridis, Anadara antiquata, Penaeus monodon, Litopenaeus vannamei) collected from the main traditional seafood market (Pasar Pabean), Surabaya, Indonesia. The aim of the study was to determine and evaluate the level of bacterial contamination if any, found in shellfish and shrimp sold at Pabean market. The total number of Escherichia coli in green mussel (Pernaviridis) samples was about 21,500 CFU/mL and the lowest was recorded in tiger prawn samples (Penaeus monodon) which was 2,800 CFU/mL. The highest Salmonella concentration was obtained from blood clam (Anadara granosa) samples which contained 470 CFU/mL however it was not present in shrimp samples. The highest concentration of Vibrio cholerae was obtained from the antique ark (Anadara antiquata) samples and was about 120 CFU/mL but was absent in green mussels samples, tiger prawn, and vannamei shrimp. In all, it can be concluded that the concentration of Escherichia coli obtained from all the seafood samples in this research exceeds the threshold for human consumption. All the shellfish samples contained Salmonella but Vibrio cholera was only present in two species including blood clam and antique ark.
JIPK (JURNAL ILMIAH PERIKANAN DAN KELAUTAN), 2020
There is a need to have a single document that summarises the present day uses of Spirulina. In t... more There is a need to have a single document that summarises the present day uses of Spirulina. In this review, the research trend on the health and other applications of Spirulina microalga was critically evaluated. In terms of the health uses, antiox-idant, antibacterial, and immunostimulant effects of Spirulina were emphasized. Other uses of the microalga discussed include the use of Spirulina for human and animal food, bioenergy, pollution and ecotoxicology control, cosmetics, bioplas-tics and biofertilizers. Literature search revealed that Spirulina polysaccharides, phycocyanin size and content play a role in antioxidant activity and DNA repair. The double bonds and positions of-COOH and-OH in Spirulina phenol content and γ-linolenic fatty acids (γ-LFA) have antimicrobial activity. Some compounds in Spirulina improve immune, increase survival rate and enhance distribution of proteins like hepcidin and TNF-α in animal models. High protein, vitamins, fatty acids (FAs) and glycoproteins in Spirulina are easily digestible due to its lack of cellulose and can improve human and livestock growth. Spirulina produces biodegradable and non-toxic biodiesel and useful co-products. Absorption of heavy metals by chemisorption occurs in Spirulina. Phycocyanin and β-carotene of Spi-rulina increase skin health, Spirulina also cause high cell proliferation and aids wound healing. Bioplastics produced from Spirulina are biodegradable, non-toxic with high blends. Biofertilizers from Spirulina have little or no residual risks, adds soil Nitrogen through Spirulina Nitrogen fixation ability. In addition, the survey of published works on Spirulina for the past two decades indicates that more research is been carried out in recent years using Spirulina, especially studies involving its health potentials and those concerned with molecular analysis. In conclusion, Spirulina is an exceptional commodity with numerous applications, and probably, some of its compounds causing those effects are yet to be isolated and that is one area for further research.
2nd International Conference Postgraduate School (ICPS 2018), 2018
This study was aimed at identifying the fish habitat that harbors O. niloticus with higher nutrie... more This study was aimed at identifying the fish habitat that harbors O. niloticus with higher nutrients and availing these findings for nutritional guidance. Amino acid and proximate compositions of O. niloticus from Lower Benue River and UAM fish farm were determined monthly for a 3month period, using methods of Benitez (1989) and AOAC (2006) respectively. The mean of Glutamic acid (12.51±0.64 and 11.85±0.67) and Alanine (5.40±0.23 and 4.50±0.41) varied significantly (p<0.05) between the wild and cultured while other amino acids were not. In October, ash (4.62±0.02 and 3.69±0.02), fat (4.71±0.06 and 3.61±0.02), protein (14.62±0.07 and 9.98±0.02) and moisture (75.67±0.07 and 79.65±0.15) varied significantly between the wild and cultured O. niloticus. Crude protein (20.46±0.01 and 18.75±0.04) and moisture (69.32±0.02 and 73.35±0.20) significantly varied in November between the samples respectively. Only crude protein (18.89±0.04 and 20.31±0.06) varied statistically in December between wild and cultured. The mean of crude protein (17.99±1.22 and 16.34±1.86) and the mean moisture (72.53±1.16 and 74.94±1.52) differed significantly between the wild and cultured. From these results. O. niloticus wild expressed superiority over the cultured, however, both River Benue at Makurdi and the UAM fish farm harbor nutritious O. niloticus which is good for human consumption based on our daily amino acid and protein needs.
Amino acid and proximate analysis of Clarias gariepinus from Lower Benue River and the earthen po... more Amino acid and proximate analysis of Clarias gariepinus from Lower Benue River and the earthen ponds were carried out using standard methods. Apart from Leucine, all the other 16 amino acids analyzed did not vary statistically between C. gariepinus from Lower Benue River and the cultured one. On monthly basis, proximate parameters varied significantly between C. gariepinus from Lower Benue River and the pond with that of the wild being highest in most parameters examined. Mean values of all proximate parameters studied were not statistically significant apart from the mean moisture content, which varied between wild and pond C. gariepinus (75.92±0.48 and 78.50 ±0.26) respectively. The results of this study clearly indicate that fish sampled from these two sources have adequate protein and amino acid levels required by man on daily basis as stated by FAO/WHO/UN in even less than 10g wet weight.
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Papers by Aondohemba S Nege