V[(mmol/L)min-1] 1/V

[S] (mmol/L) Sin inhibidor Inhibidor A 1/[S] (L/mmol)Sin inhibidor Con inhibidor
1.25 1.72 0.98 0.8 0.58139535 1.02040816
1.67 2.04 1.17 0.5988024 0.49019608 0.85470085
2.5 2.63 1.47 0.4 0.38022814 0.68027211
5 3.33 1.96 0.2 0.3003003 0.51020408
10 4.17 2.38 0.1 0.23980815 0.42016807

LINEWEAVER-BRUK
1.2
y = 0.8582x + 0.3369
1 R² = 0.9999

0.8

0.6

1/V
0.4

0.2 y = 0.4843x + 0.1951

R² = 0.9976
0
-1 -0.5 0 0.5 1
-0.2

-0.4

-0.6
1/[S]

1 𝐾 =𝑚∗𝑉
𝑉 =
𝑏

a)TIPO DE INHIBICION: NO COMPETITIVA

b) Vmax y Km
SIN INHIBIDOR
y = 0.4843x + 0.1951
m= 0.4843 Vmax= 5.12557663
b= 0.1951 Km= 2.48231676

CON INHIBIDOR
y = 0.8582x + 0.3369
m= 0.8582 Vmax= 2.96823983
b= 0.3369 Km= 2.54734343
 V[(mmol/L)min-1] 1/V
[S] (mmol/L) Sin inhibidor Inhibidor B 3mMInhibidor B 5mM 1/[S] (L/mmol)Sin inhibidor Con inhibidor BCon
3mMinhibidor
1.25 1.72 1.25 1.01 0.8 0.58139535 0.8 0.99009901
1.67 2.04 1.54 1.26 0.5988024 0.49019608 0.64935065 0.79365079
2.5 2.63 2 1.72 0.4 0.38022814 0.5 0.58139535
5 3.33 2.86 2.56 0.2 0.3003003 0.34965035 0.390625
10 4.17 3.7 3.49 0.1 0.23980815 0.27027027 0.28653295

Lineweaver-burk
0.9
0.8 y = 0.755x + 0.1969
0.7 R² = 0.9999
0.6
0.5
0.4 y = 0.4843x + 0.1951

1/V
0.3 R² = 0.9976
0.2
0.1
0
-0.6 -0.4 -0.2 -0.1 0 0.2 0.4 0.6 0.8 1
-0.2
1/[S]

1 𝐾 =𝑚∗𝑉
𝑉 =
𝑏

a)TIPO DE INHIBICION: COMPETITIVA

𝐼
𝐾
b) Vmax y Km c)KI 𝐾 = −1
𝐾
SIN INHIBIDOR
y=0.4843x+0.1951
m= 0.4843 Vmax= 5.12557663 mM/min
b= 0.1951 Km= 2.48231676 mM

CON INHIBIDOR B 3mM

y = 0.755x + 0.1969
m= 0.755 Vmax= 5.07872016 mM/min KI= 5.50762285
b= 0.1969 Km= 3.83443372 mM

CON INHIBIDOR B 5mM

y = 1.0062x + 0.1861
m= 1.0062 Vmax= 5.37345513 mM/min KI= 4.24406904
b= 0.1861 Km= 5.40677055 mM
Es un inhibidor no competitivo, principalmente por la notable
disminucion del valor de Vmax y que ademas a simple vista pareciera
que la Km va a permanecer igual.
 MICHAELIS MENTEN LINEWEAVER-BRUK
[ ] L-glutamato (mM)
V0 (cambio de absorbancia a 360 nm/min) 1/[S] 1/V0
1.68 0.172 0.5952381 5.81395349
3.33 0.25 0.3003003 4
5 0.286 0.2 3.4965035
6.67 0.303 0.14992504 3.30033003
10 0.334 0.1 2.99401198
20 0.384 0.05 2.60416667

Michaelis menten V0 vs [S] Lineweaver-burk 1/V0 vs 1/[S]

0.5 7
y = 5.7477x + 2.3647

Velocidad (nM/min)
6
0.4 R² = 0.9969
5
0.3
4

[S]/V0
0.2 3
0.1 2
1
0
0
0 5 10 15 20 25
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
[S] (mM) S

. Vmax Km
Lineweaver-bruk0.42288662 2.42938639 1 𝐾 1 1
Hanes-Wolf 0.43094161 2.60629175 = +
𝑉 𝑉 𝑆 𝑉
Eadie-Hofstee 0.422 2.413
y=5.7477x+2.3647
m= 5.74477
1 b= 2.3647
𝑉 =
𝑏 Vmax= 0.42288662

𝐾 =𝑚∗𝑉
Km= 2.42938639
 HANES-WOLF EADIE HOFSTEE
[S] [S]/V0 V/[S] V
1.68 9.76744186 1.02E-01 0.172
3.33 13.32 7.51E-02 0.25
5 17.4825175 5.72E-02 0.286
6.67 22.0132013 4.54E-02 0.303
10 29.9401198 3.34E-02 0.334
20 52.0833333 1.92E-02 0.384

HANES-WOLF [S]/V0 VS [S] EADIE HOFSTEE

60 0.45
0.4
50 y = 2.3205x + 6.0479 0.35
R² = 0.9991
40 0.3
[S]/V0

0.25
30

V0
0.2
20 0.15 y = -2.413x + 0.422
R² = 0.9883
10 0.1
0.05
0 0
0 5 10 15 20 25 0.00E+00 2.00E-02 4.00E-02 6.00E-02 8.00E-02 1.00E-01 1.20E-01
[S]/V0
V/[S]

𝑆 𝐾 1 𝑉
= + ∗ 𝑆 𝑉0 = 𝑉 −𝐾
𝑉 𝑉 𝑉 ¨[𝑆]¨

y=2.3205x+6.0479 y = -2.413x+0.422
m= 2.3205 m= -2.41E+00
b= 6.0479 b= 0.422

1 Vmax=b Vmax= 0.422

𝑉 =
𝑚 Vmax= 0.43094161

𝐾 =𝑏∗𝑉 Km=-m Km= 2.413

Km= 2.60629175
[S] (mM) V0(mM*min-1)V0 con I presente (mM*min-1) 1/[S] (L/mmol)1/V0(mM*min-1)
1/V0 CON I
1 1.3 0.8 1 0.76923077 1.25
2 2 1.2 0.5 0.5 0.83333333
4 2.8 1.7 0.25 0.35714286 0.58823529
8 3.6 2.2 0.125 0.27777778 0.45454545
12 4 2.4 0.08333333 0.25 0.41666667

Título del gráfico

1.4
y = 0.9113x + 0.3516
1.2
R² = 0.9975
1

0.8

Título del eje

0.6

0.4
y = 0.564x + 0.2099
R² = 0.999
0.2

0
-0.6 -0.4 -0.2 0 0.2 0.4 0.6 0.8 1 1.2
-0.2
Título del eje

Inhibicion: No competitiva
[Esfingosina] (mM)
V0(mg*min-1)V0(mg*min-1) con treo-esingosina 1/[Esfingosina]1/V0(mg*min-1)
(mM) 1/V0(mg*min-1)
2.5 32.3 8.5 0.4 0.03095975 0.11764706
3.5 40 11.5 0.28571429 0.025 0.08695652
5 50.8 14.6 0.2 0.01968504 0.06849315
10 72 25.4 0.1 0.01388889 0.03937008
20 87.7 43.9 0.05 0.01140251 0.02277904
50 115.4 70.8 0.02 0.00866551 0.01412429

a) GRAFICO

Lineweaver-burk
0.15
y = 0.2668x + 0.0118
R² = 0.9966
0.1

y = 0.0567x + 0.0084
0.05 R² = 0.9991

1/V
0
-0.4 -0.3 -0.2 -0.1 0 0.1 0.2 0.3 0.4 0.5

-0.05

-0.1
1/[S]

c)TIPO DE INHIBICION: COMPETITIVA

b) Vmax y Km
SIN INHIBIDOR
y=0.0567x+0.0084
m= 0.00567 Vmax= 119.047619 mM/min
b= 0.0084 Km= 0.675 mM

CON INHIBIDOR B
y=0.2668x+0.0118
m= 0.2668 Vmax= 84.7457627 mM/min
b= 0.0118 Km= 22.6101695 mM