Abstract A computational method for predicting the dispersion of gaseous emissions in regions wit... more Abstract A computational method for predicting the dispersion of gaseous emissions in regions with complex topography is presented. Different meteorological conditions, source data, and gas compositions and specific weights are taken into consideration. The three-dimensional governing equations of fluid- and thermodynamics are numerically solved by means of the finite volume method. The kɛ turbulence model is utilized to account for the turbulent nature of the flow. The numerical results obtained by the proposed method show satisfactory agreement with both results obtained by other numerical methods and experimental and/or measured data. The presented method is applied to four test cases, including steady and unsteady problems, in order to illustrate its usefulness.
Autophagy plays an important role in cancer cells. Targeting autophagy in cancer can provide new ... more Autophagy plays an important role in cancer cells. Targeting autophagy in cancer can provide new opportunities for drug development. In this study we tested four Schiff base Cu(II) complexes against human breast cancer cells (MCF-7) and human non-cancerous cells (HEK-293T). We have tested their cytotoxic effect by evaluating IC using MTT test. To detect morphological changes of the actin fibers we have used fluorescent microscopy. To determine the type of cell death we used electrophoretic analysis and western blot analysis (protein LC3). IC values of the complexes increased with time of their influence, indicating acquired resistance of MCF-7 to the complexes. Healthy cells HEK-293T were not sensitive to the Cu(II) complexes. Compared with the control cells (cells without Cu(II) complexes) which were without morphological changes of actin fibers, Cu(II) complexes induced condensation and asymmetric conformational changes in actin filaments. To examine the type of cell death induced by the Cu(II) complexes we treated MCF-7 cells with Cu(II) complexes (1, 10, 50 and 100μmol/L) during a 72h incubation period. By electrophoresis we have not detected any DNA fragmentation. To determine whether Cu(II) complexes induced autophagy or necrotic cell death we used the western blot analysis. MCF-7 cells influenced with tested Cu(II) complexes produced LC3 protein after their 72h incubation indicating autophagy in MCF-7 cancer cells. Tested Schiff base copper (II) complexes have antiproliferative activity against cancer cells but not against healthy cells. They have induced autophagy in the cancer cell line MCF-7.
Numerical analysis has been performed for predicting the onset and establishment of a steady stat... more Numerical analysis has been performed for predicting the onset and establishment of a steady state flow of a reactive hydrogen/air/vapour mixture through a two-dimensional vertical duct of finite length with its side walls coated by catalytic material. The flow is initiated by the exothermic reaction of hydrogen with air oxygen on the catalytic wall, that causes the hot gases to flow upwards through the vertical duct and by continuity sucks fresh mixture through the lower end of the duct. The flow is always laminar and the two-dimensional governing transport differential equations are solved by means of the numerical finite volume method, using a collocated variable arrangement. Comparisons between calculated and experimental data are presented, showing good agreement between them. The method is employed for various initial mixture compositions and duct geometries.
A computational method for predicting the dispersion of gaseous emissions in regions with complex... more A computational method for predicting the dispersion of gaseous emissions in regions with complex topography is presented. Different meteorological conditions, source data, and gas compositions are taken into consideration. The three-dimensional governing equations of fluid- and thermodynamics are numerically solved by means of the finite volume method. The k-ffl turbulence model is utilized to account for the turbulent nature of the flow. Numerical results show satisfactory agreement with experimental data. The presented method is applied to three test cases, including steady and unsteady problems, in order to illustrate its usefulness. z Technical University of Munich x Rice University NUMERICAL SIMULATION OF THE THREE-DIMENSIONALMICRO-SCALE DISPERSION OF AIR-POLLUTANTS IN REGIONS WITH COMPLEX TOPOGRAPHY Georgios A. Perdikaris -- and Michael Kokkolaras k Department of Mechanical Engineering and Materials Science, William Marsh Rice University 6100 Main Houston, TX 77005-1892...
Abstract A computational method for predicting the dispersion of gaseous emissions in regions wit... more Abstract A computational method for predicting the dispersion of gaseous emissions in regions with complex topography is presented. Different meteorological conditions, source data, and gas compositions and specific weights are taken into consideration. The three-dimensional governing equations of fluid- and thermodynamics are numerically solved by means of the finite volume method. The kɛ turbulence model is utilized to account for the turbulent nature of the flow. The numerical results obtained by the proposed method show satisfactory agreement with both results obtained by other numerical methods and experimental and/or measured data. The presented method is applied to four test cases, including steady and unsteady problems, in order to illustrate its usefulness.
Autophagy plays an important role in cancer cells. Targeting autophagy in cancer can provide new ... more Autophagy plays an important role in cancer cells. Targeting autophagy in cancer can provide new opportunities for drug development. In this study we tested four Schiff base Cu(II) complexes against human breast cancer cells (MCF-7) and human non-cancerous cells (HEK-293T). We have tested their cytotoxic effect by evaluating IC using MTT test. To detect morphological changes of the actin fibers we have used fluorescent microscopy. To determine the type of cell death we used electrophoretic analysis and western blot analysis (protein LC3). IC values of the complexes increased with time of their influence, indicating acquired resistance of MCF-7 to the complexes. Healthy cells HEK-293T were not sensitive to the Cu(II) complexes. Compared with the control cells (cells without Cu(II) complexes) which were without morphological changes of actin fibers, Cu(II) complexes induced condensation and asymmetric conformational changes in actin filaments. To examine the type of cell death induced by the Cu(II) complexes we treated MCF-7 cells with Cu(II) complexes (1, 10, 50 and 100μmol/L) during a 72h incubation period. By electrophoresis we have not detected any DNA fragmentation. To determine whether Cu(II) complexes induced autophagy or necrotic cell death we used the western blot analysis. MCF-7 cells influenced with tested Cu(II) complexes produced LC3 protein after their 72h incubation indicating autophagy in MCF-7 cancer cells. Tested Schiff base copper (II) complexes have antiproliferative activity against cancer cells but not against healthy cells. They have induced autophagy in the cancer cell line MCF-7.
Numerical analysis has been performed for predicting the onset and establishment of a steady stat... more Numerical analysis has been performed for predicting the onset and establishment of a steady state flow of a reactive hydrogen/air/vapour mixture through a two-dimensional vertical duct of finite length with its side walls coated by catalytic material. The flow is initiated by the exothermic reaction of hydrogen with air oxygen on the catalytic wall, that causes the hot gases to flow upwards through the vertical duct and by continuity sucks fresh mixture through the lower end of the duct. The flow is always laminar and the two-dimensional governing transport differential equations are solved by means of the numerical finite volume method, using a collocated variable arrangement. Comparisons between calculated and experimental data are presented, showing good agreement between them. The method is employed for various initial mixture compositions and duct geometries.
A computational method for predicting the dispersion of gaseous emissions in regions with complex... more A computational method for predicting the dispersion of gaseous emissions in regions with complex topography is presented. Different meteorological conditions, source data, and gas compositions are taken into consideration. The three-dimensional governing equations of fluid- and thermodynamics are numerically solved by means of the finite volume method. The k-ffl turbulence model is utilized to account for the turbulent nature of the flow. Numerical results show satisfactory agreement with experimental data. The presented method is applied to three test cases, including steady and unsteady problems, in order to illustrate its usefulness. z Technical University of Munich x Rice University NUMERICAL SIMULATION OF THE THREE-DIMENSIONALMICRO-SCALE DISPERSION OF AIR-POLLUTANTS IN REGIONS WITH COMPLEX TOPOGRAPHY Georgios A. Perdikaris -- and Michael Kokkolaras k Department of Mechanical Engineering and Materials Science, William Marsh Rice University 6100 Main Houston, TX 77005-1892...
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