To overcome limitations induced by vagueness and uncertainty of real life data, neoclassical anal... more To overcome limitations induced by vagueness and uncertainty of real life data, neoclassical analysis [10] has been developed. It extends the scope and results of classical mathematical analysis by applying fuzzy logic to conventional mathematical objects, such as functions, ...
Several reports suggested that haploid parthenogenetic embryos have less developmental ability as... more Several reports suggested that haploid parthenogenetic embryos have less developmental ability as compared to diploid parthenogenetic or IVF derived embryos, may be due to lesser expression of developmental related genes. The present study was, therefore, carried out to compare expression of early embryonic developmental related genes in different stages of haploid and diploid parthenogenetic embryos in goat. Good quality oocytes were aspirated from the ovary and matured in maturation medium without (haploid embryo generation) or with cytochalasin B (for diploid embryo generation). After maturation, oocytes were activated using ionomycin followed by incubation with 6-DMAP in mSOF. The activated oocytes were further cultured in mSOF for generation of parthenogenetic embryos of different stages. Total RNA was isolated from pool of, viz 2–4, 8–16 and morula cell stages embryos of haploid and diploid, respectively, and cDNA was synthesized. The relative expression of Nanog , Sox -2, Klf 4 and Fox d3 genes were analyzed by real time PCR. The result indicated that the relative expression of all 4 genes could not be detected in 2–4 and 8–16 cell stage of haploid embryos, whereas 2–4 and 8–16 cell stage diploid embryos expressed all these genes. When both type of embryos reached at morula stage, the expression of all the genes were present and no significant changes were observed between haploid and diploid parthenogenetic embryos. From the results, it can be concluded that haploid parthenogenetic embryos showed undetectable level of embryonic develpomenta lrelated genes like Nanog , Sox -2, Klf 4 and Foxd3 at early embryonic develpomental stage in comparison to diploid parthenogenetic embryos. This may be one of the major reasons for poor developmental competence of parthenogenetic haploid embryos.
Thirty four genotypes were studied for their genotypic, phenotypic and environmental coefficient ... more Thirty four genotypes were studied for their genotypic, phenotypic and environmental coefficient of variation during wet season 1999–2000. Results indicated that phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV) ...
In this paper, we provide theoretical arguments and empirical evidence for how Genetic Algorithms... more In this paper, we provide theoretical arguments and empirical evidence for how Genetic Algorithms (GA) can be used for efficient estimation of macro-level diffusion models. Using simulations we find that GA and Sequential Search-Based-Nonlinear Least Squares (SSB-NLS) provide comparable parameter estimates when the data including peak sales are being used, for a range of error variances, and true parameter values commonly encountered in the literature. From empirical analyses we find that the forecasting performance of the GA estimates is better than that of SSB-NLS, Augmented Filter, Hierarchical Bayes, and Kalman Filter when only pre-peak sales data is available for estimation. When sales data until the peak time period are available for estimation, SSB-NLS is able to obtain parameter estimates when the starting values provided are the estimates from using GA. The estimates from GA are not biased and do not change in a systematic fashion when post-peak sales data are used, whereas the estimates from SSB-NLS are biased and change in a systematic fashion. Summarizing, we find that GA may be better suited for diffusion model estimation under the three conditions where SSB-NLS has been found to have problems.
For an admissible ideal I of subsets of N, the set of positive integers, the concept of I-converg... more For an admissible ideal I of subsets of N, the set of positive integers, the concept of I-convergence was introduced in (8) and studied by many authors. The aim of this paper is to introduce the notions of I-Cauchy and I?-Cauchy sequences on probabilistic normed spaces. Furthermore, we study some fundamental properties
The concepts of I-limit points and I-cluster points are natural generalization of the concepts of... more The concepts of I-limit points and I-cluster points are natural generalization of the concepts of statistical limit points and statistical cluster points, respectively, for real sequences. In the present paper we introduce the notions of I-limit points and I-cluster points for sequences of fuzzy numbers and continue with this study.
The "CyberBridges" pilot project is an innovative model for creating a new generation of scientis... more The "CyberBridges" pilot project is an innovative model for creating a new generation of scientists and engineers who are capable of fully integrating cyberinfrastructure into the whole educational, professional, and creative process of their respective disciplines. CyberBridges augments graduate student education to include a foundation of understanding in Advanced Networking and Grid Infrastructure for High Performance Computing, and bridges the divide between the information technology community and diverse science and engineering disciplines. We demonstrate the effectiveness of CyberBridges by providing four case studies. Groundwork has begun to extend the outreach of CyberBridges for international research and education collaborations.
The notion of ideal convergence of single and double sequences were introduced in (16) and (17), ... more The notion of ideal convergence of single and double sequences were introduced in (16) and (17), respectively. This notion includes the notion of statistical convergence which has been intensively investigated in last twenty years. In this paper we de…ne and study ideal analogue of convergence and Cauchy sequences on intuitionistic fuzzy normed spaces(IFNS for short) and establish some properties related to these notions.
The present study aimed at exploring the linkages between Allen and Meyer's (1991) three componen... more The present study aimed at exploring the linkages between Allen and Meyer's (1991) three component model of organizational commitment and organizational citizenship behavior as defined by Organ (1988), using a field survey on a randomly selected sample of 77 employees working in National Hydroelectric Power Corporation Ltd., a public sector organization in India. Organizational Commitment was measured using revised version of Organizational Commitment Scale by Allen and Meyer (1997) and organizational citizenship behavior scale (OCBS) developed by Bakhshi and Kumar (2009) was used as an aggregate measure of organizational citizenship behavior (OCB). The results of partial correlation analysis showed that all the three components of organizational commitment-Affective commitment, Continuance commitment and Normative commitment were positively correlated with aggregate measure of OCB. Hierarchical Regression analyses showed no significant impact of demographic variables (age, gender, job tenure, marital status and qualification) on aggregate measure of OCB. Among the three components of organizational commitment, only normative commitment has a significant positive impact on aggregate measure of OCB. Theoretical and practical implications of the results are discussed.
... Govt. & Private Colleges in Jammu. Dr. Arti Bakhshi (Reader) Kuldeep Kumar (JRF) Ph. 0946... more ... Govt. & Private Colleges in Jammu. Dr. Arti Bakhshi (Reader) Kuldeep Kumar (JRF) Ph. 09469212552, e-mail: [email protected] Shallu Sharma (Res. Scholar) Ambica Sharma (Res. Scholar) University of Jammu. Abstract ...
International Journal of System Assurance Engineering and Management, 2011
Conventional reliability analysis relies on the probability model, which is often inappropriate f... more Conventional reliability analysis relies on the probability model, which is often inappropriate for handling complex systems due to the lack of sufficient probabilistic information. For large complex systems made up of many components, the uncertainty of each individual parameter amplifies the uncertainty of the total system reliability. To overcome this problem, the concept of fuzzy approach has been used in the evaluation of the reliability of a system. In this article, we use a biscuit manufacturing plant consisting of six subsystems to capture the effect of coverage factor, failure and repair on its fuzzy availability. Mathematical formulation of the problem is carried out using mnemonic rule and the governing differential equations are solved using Matlab 7.4 by Runge-Kutta method of order four (Ode 45 function).
Mesenchymal stromal cells (MSC) are multipotent cells that can be derived from many different org... more Mesenchymal stromal cells (MSC) are multipotent cells that can be derived from many different organs and tissues. While there are many ways to label and track cells each with strengths and weakness, the green fluorescent protein (GFP) is a reporter gene commonly employed. In the present study, caprine MSC were collected from bone marrow and cells were characterised with MSC specific markers. Passage 10 (P10) MSC cells were transfected using plasmid vector containing GFP as reporter gene with different concentrations of DNA and lipofectamine. Six different concentrations of DNA and lipofectamine as 1 microg DNA: 2 microL lipofectamine, 1 microg DNA: 2.5 microL lipofectamine, 1.2 microg DNA: 2.2 microL lipofectamine, 1.2 microg DNA: 2.5 microL lipofectamine, 1.5 microg DNA: 2.5 microL lipofectamine, 1.5 microg DNA: 3 microL lipofectamine were used. After 24 h and 48 h of transfection, caprine MSC were observed under florescent microscope. Highest transfection rate indicating green flo...
The production of cells capable of expressing gene(s) of interest is important for a variety of a... more The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and a novel method of stem cell therapy in the various diseases. Achieving high levels of transgene expression for the longer period of time, without adversely affecting cell viability and differentiation capac-ity of the cells, is crucial. In the present study, we investigated the efficiency of plasmid vector for the production of transgenic cMSCs and examined any functional change of cells after transfection. To do so first we have collected bone marrows from the adult goats and cultured them for isolation of mesenchymal stem cells (cBM-MSCs). These cells were characterized us-ing MSC specific markers including differentia-tion into osteocytes and adipocytes. Transfec-tion with plasmid vector did not adversely affect cBM-MSCs morphology, viability or differentia-tion potential, and transgene expression levels were una...
This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy an... more This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species.
To overcome limitations induced by vagueness and uncertainty of real life data, neoclassical anal... more To overcome limitations induced by vagueness and uncertainty of real life data, neoclassical analysis [10] has been developed. It extends the scope and results of classical mathematical analysis by applying fuzzy logic to conventional mathematical objects, such as functions, ...
Several reports suggested that haploid parthenogenetic embryos have less developmental ability as... more Several reports suggested that haploid parthenogenetic embryos have less developmental ability as compared to diploid parthenogenetic or IVF derived embryos, may be due to lesser expression of developmental related genes. The present study was, therefore, carried out to compare expression of early embryonic developmental related genes in different stages of haploid and diploid parthenogenetic embryos in goat. Good quality oocytes were aspirated from the ovary and matured in maturation medium without (haploid embryo generation) or with cytochalasin B (for diploid embryo generation). After maturation, oocytes were activated using ionomycin followed by incubation with 6-DMAP in mSOF. The activated oocytes were further cultured in mSOF for generation of parthenogenetic embryos of different stages. Total RNA was isolated from pool of, viz 2–4, 8–16 and morula cell stages embryos of haploid and diploid, respectively, and cDNA was synthesized. The relative expression of Nanog , Sox -2, Klf 4 and Fox d3 genes were analyzed by real time PCR. The result indicated that the relative expression of all 4 genes could not be detected in 2–4 and 8–16 cell stage of haploid embryos, whereas 2–4 and 8–16 cell stage diploid embryos expressed all these genes. When both type of embryos reached at morula stage, the expression of all the genes were present and no significant changes were observed between haploid and diploid parthenogenetic embryos. From the results, it can be concluded that haploid parthenogenetic embryos showed undetectable level of embryonic develpomenta lrelated genes like Nanog , Sox -2, Klf 4 and Foxd3 at early embryonic develpomental stage in comparison to diploid parthenogenetic embryos. This may be one of the major reasons for poor developmental competence of parthenogenetic haploid embryos.
Thirty four genotypes were studied for their genotypic, phenotypic and environmental coefficient ... more Thirty four genotypes were studied for their genotypic, phenotypic and environmental coefficient of variation during wet season 1999–2000. Results indicated that phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV) ...
In this paper, we provide theoretical arguments and empirical evidence for how Genetic Algorithms... more In this paper, we provide theoretical arguments and empirical evidence for how Genetic Algorithms (GA) can be used for efficient estimation of macro-level diffusion models. Using simulations we find that GA and Sequential Search-Based-Nonlinear Least Squares (SSB-NLS) provide comparable parameter estimates when the data including peak sales are being used, for a range of error variances, and true parameter values commonly encountered in the literature. From empirical analyses we find that the forecasting performance of the GA estimates is better than that of SSB-NLS, Augmented Filter, Hierarchical Bayes, and Kalman Filter when only pre-peak sales data is available for estimation. When sales data until the peak time period are available for estimation, SSB-NLS is able to obtain parameter estimates when the starting values provided are the estimates from using GA. The estimates from GA are not biased and do not change in a systematic fashion when post-peak sales data are used, whereas the estimates from SSB-NLS are biased and change in a systematic fashion. Summarizing, we find that GA may be better suited for diffusion model estimation under the three conditions where SSB-NLS has been found to have problems.
For an admissible ideal I of subsets of N, the set of positive integers, the concept of I-converg... more For an admissible ideal I of subsets of N, the set of positive integers, the concept of I-convergence was introduced in (8) and studied by many authors. The aim of this paper is to introduce the notions of I-Cauchy and I?-Cauchy sequences on probabilistic normed spaces. Furthermore, we study some fundamental properties
The concepts of I-limit points and I-cluster points are natural generalization of the concepts of... more The concepts of I-limit points and I-cluster points are natural generalization of the concepts of statistical limit points and statistical cluster points, respectively, for real sequences. In the present paper we introduce the notions of I-limit points and I-cluster points for sequences of fuzzy numbers and continue with this study.
The "CyberBridges" pilot project is an innovative model for creating a new generation of scientis... more The "CyberBridges" pilot project is an innovative model for creating a new generation of scientists and engineers who are capable of fully integrating cyberinfrastructure into the whole educational, professional, and creative process of their respective disciplines. CyberBridges augments graduate student education to include a foundation of understanding in Advanced Networking and Grid Infrastructure for High Performance Computing, and bridges the divide between the information technology community and diverse science and engineering disciplines. We demonstrate the effectiveness of CyberBridges by providing four case studies. Groundwork has begun to extend the outreach of CyberBridges for international research and education collaborations.
The notion of ideal convergence of single and double sequences were introduced in (16) and (17), ... more The notion of ideal convergence of single and double sequences were introduced in (16) and (17), respectively. This notion includes the notion of statistical convergence which has been intensively investigated in last twenty years. In this paper we de…ne and study ideal analogue of convergence and Cauchy sequences on intuitionistic fuzzy normed spaces(IFNS for short) and establish some properties related to these notions.
The present study aimed at exploring the linkages between Allen and Meyer's (1991) three componen... more The present study aimed at exploring the linkages between Allen and Meyer's (1991) three component model of organizational commitment and organizational citizenship behavior as defined by Organ (1988), using a field survey on a randomly selected sample of 77 employees working in National Hydroelectric Power Corporation Ltd., a public sector organization in India. Organizational Commitment was measured using revised version of Organizational Commitment Scale by Allen and Meyer (1997) and organizational citizenship behavior scale (OCBS) developed by Bakhshi and Kumar (2009) was used as an aggregate measure of organizational citizenship behavior (OCB). The results of partial correlation analysis showed that all the three components of organizational commitment-Affective commitment, Continuance commitment and Normative commitment were positively correlated with aggregate measure of OCB. Hierarchical Regression analyses showed no significant impact of demographic variables (age, gender, job tenure, marital status and qualification) on aggregate measure of OCB. Among the three components of organizational commitment, only normative commitment has a significant positive impact on aggregate measure of OCB. Theoretical and practical implications of the results are discussed.
... Govt. & Private Colleges in Jammu. Dr. Arti Bakhshi (Reader) Kuldeep Kumar (JRF) Ph. 0946... more ... Govt. & Private Colleges in Jammu. Dr. Arti Bakhshi (Reader) Kuldeep Kumar (JRF) Ph. 09469212552, e-mail: [email protected] Shallu Sharma (Res. Scholar) Ambica Sharma (Res. Scholar) University of Jammu. Abstract ...
International Journal of System Assurance Engineering and Management, 2011
Conventional reliability analysis relies on the probability model, which is often inappropriate f... more Conventional reliability analysis relies on the probability model, which is often inappropriate for handling complex systems due to the lack of sufficient probabilistic information. For large complex systems made up of many components, the uncertainty of each individual parameter amplifies the uncertainty of the total system reliability. To overcome this problem, the concept of fuzzy approach has been used in the evaluation of the reliability of a system. In this article, we use a biscuit manufacturing plant consisting of six subsystems to capture the effect of coverage factor, failure and repair on its fuzzy availability. Mathematical formulation of the problem is carried out using mnemonic rule and the governing differential equations are solved using Matlab 7.4 by Runge-Kutta method of order four (Ode 45 function).
Mesenchymal stromal cells (MSC) are multipotent cells that can be derived from many different org... more Mesenchymal stromal cells (MSC) are multipotent cells that can be derived from many different organs and tissues. While there are many ways to label and track cells each with strengths and weakness, the green fluorescent protein (GFP) is a reporter gene commonly employed. In the present study, caprine MSC were collected from bone marrow and cells were characterised with MSC specific markers. Passage 10 (P10) MSC cells were transfected using plasmid vector containing GFP as reporter gene with different concentrations of DNA and lipofectamine. Six different concentrations of DNA and lipofectamine as 1 microg DNA: 2 microL lipofectamine, 1 microg DNA: 2.5 microL lipofectamine, 1.2 microg DNA: 2.2 microL lipofectamine, 1.2 microg DNA: 2.5 microL lipofectamine, 1.5 microg DNA: 2.5 microL lipofectamine, 1.5 microg DNA: 3 microL lipofectamine were used. After 24 h and 48 h of transfection, caprine MSC were observed under florescent microscope. Highest transfection rate indicating green flo...
The production of cells capable of expressing gene(s) of interest is important for a variety of a... more The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and a novel method of stem cell therapy in the various diseases. Achieving high levels of transgene expression for the longer period of time, without adversely affecting cell viability and differentiation capac-ity of the cells, is crucial. In the present study, we investigated the efficiency of plasmid vector for the production of transgenic cMSCs and examined any functional change of cells after transfection. To do so first we have collected bone marrows from the adult goats and cultured them for isolation of mesenchymal stem cells (cBM-MSCs). These cells were characterized us-ing MSC specific markers including differentia-tion into osteocytes and adipocytes. Transfec-tion with plasmid vector did not adversely affect cBM-MSCs morphology, viability or differentia-tion potential, and transgene expression levels were una...
This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy an... more This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species.
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