Papers by Giuseppina Mulè
Toxins, 2018
MycoKey, an EU-funded Horizon 2020 project, includes a series of "Roundtable Discussions&quo... more MycoKey, an EU-funded Horizon 2020 project, includes a series of "Roundtable Discussions" to gather information on trending research areas in the field of mycotoxicology. This paper includes summaries of the Roundtable Discussions on Chemical Detection and Monitoring of mycotoxins and on the role of genetics and biodiversity in mycotoxin production. Discussions were managed by using the nominal group discussion technique, which generates numerous ideas and provides a ranking for those identified as the most important. Four questions were posed for each research area, as well as two questions that were common to both discussions. Test kits, usually antibody based, were one major focus of the discussions at the Chemical Detection and Monitoring roundtable because of their many favorable features, e.g., cost, speed and ease of use. The second area of focus for this roundtable was multi-mycotoxin detection protocols and the challenges still to be met to enable these protocols ...
Developments in Plant Pathology, 1997
Mycotoxins, Wood Decay, Plant Stress, Biocorrosion, and General Biodeterioration, 1994
Mycologia
As part of a study on the ochratoxin producing mycoflora of grapes, several Aspergillus strains w... more As part of a study on the ochratoxin producing mycoflora of grapes, several Aspergillus strains were isolated and tested for their ochratoxin A (OTA) producing abilities. Aspergillus strains of the section Nigri, which did not produce detectable amounts of OTA but which had a similar morphology to A. carbonarius, were isolated from wine grapes and/or dried vine fruit in Portugal and Spain. These strains, however, have characters that allow morphological distinction from the other species in the section, particularly the conidia size (5-7 microm), which allows separation of the species from the two most common biseriate species in section Nigri: A. carbonarius (7-9 microm) and A. niger and its aggregate species (3-5 microm). The strains are described here as belonging to a new species, named A. ibericus. The validation of this new taxon is supported further by analysis of the ITS-5.8S rDNA and calmodulin gene sequences and by analysis of the amplified fragment length polymorphism (AF...
FEMS microbiology letters, Jan 30, 2004
Fusarium proliferatum and Fusarium oxysporum are the causal agents of a destructive disease of as... more Fusarium proliferatum and Fusarium oxysporum are the causal agents of a destructive disease of asparagus called Fusarium crown and root rot. F. proliferatum from asparagus produces fumonisin B1 and B2, which have been detected as natural contaminants in infected asparagus plants. Polymerase chain reaction (PCR) assays were developed for the rapid identification of F. proliferatum and F. oxysporum in asparagus plants. The primer pairs are based on calmodulin gene sequences. The PCR products from F. proliferatum and F. oxysporum were 526 and 534 bp long, respectively. The assays were successfully applied to identify both species from the vegetative part of the plants.
Keywords: Fumonisin Fum1 Fum21 Fusarium verticillioides FvVE1 Light a b s t r a c t Light is a ve... more Keywords: Fumonisin Fum1 Fum21 Fusarium verticillioides FvVE1 Light a b s t r a c t Light is a very important signal for fungi since it influences many different physiological responses. We analyzed the influence of light of varying wavelength and intensity on growth, conidiation and biosynthesis of fumonisin B 1 (FB 1 ), B 2 (FB 2 ), and B 3 (FB 3 ) by Fusarium verticillioides ITEM 10027. Wavelengths across the visible spectrum, from red (627 nm) to blue (470e455 nm), stimulated the growth and increased the fumonisin production, by up to 150 % over dark incubation. If the intensity of the 455 nm blue light increased from 200 to 1700 lx, the fumonisin biosynthesis decreased. Incubation under a short wave blue light (390 nm) showed reduced fungal growth and fumonisin production by up to 85 %. White pulsing light had no effect on growth but reduced fumonisin production to half of what observed during dark incubation. Real time reverse transcriptase (RT)-PCR was used to measure the expression level of Fum1, Fum21 and FvVE1 transcripts, which encode proteins involved in fumonisin biosynthesis. There was a significant correlation between gene expression and fumonisin production.
FEMS Microbiology Letters, 2015
Ochratoxin A (OTA) is a nephrotoxic and potentially carcinogenic mycotoxin produced by several sp... more Ochratoxin A (OTA) is a nephrotoxic and potentially carcinogenic mycotoxin produced by several species of Aspergillus and Penicillium. It is one of the major mycotoxins contaminating grain, grapes and a variety of food products, and the development of methods for reducing pre- and post-harvest contamination has drawn considerable attention. In the current study, we isolated and sequenced the genome of a novel free-living Acinetobacter strain able to degrade OTA. Biochemical studies suggest that the degradation reaction proceeds via peptide bond hydrolysis.
European Journal of Cancer Supplements, 2009
Abstracts for poster presentation 31 and Cox proportional hazard models showed that miR-221 down-... more Abstracts for poster presentation 31 and Cox proportional hazard models showed that miR-221 down-regulation was linked to tumor progression and recurrence. Conclusion: Our results suggest that progressive miR1-221 downregulation is a hallmark of metastasis and a novel prognostic marker in prostate carcinoma. This suggests that miR-221 has potential as a diagnostic marker and therapeutic target.
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Papers by Giuseppina Mulè