Papers by Dr.Pallavi Kaushik
journal published quarterly. The Aim of IJPBS is to publish peer reviewed research and review art... more journal published quarterly. The Aim of IJPBS is to publish peer reviewed research and review articles in less time without much delay in the developing field of Pharmaceutical and Biological sciences. One week from the date of manuscript submission author gets the decision of acceptance and if accepted the manuscript will be processed within 3 weeks (approx.) for publication.
Toxics
Fluoride (F) and arsenic (As) are two major contaminants of water and soil systems around the glo... more Fluoride (F) and arsenic (As) are two major contaminants of water and soil systems around the globe, causing potential toxicity to humans, plants, animals, and microbes. These contaminated soil systems can be restored by microorganisms that can tolerate toxic stress and provide rapid mineralization of soil, organic matter, and contaminants, using various tolerance mechanisms. Thus, the present study was undertaken with the arsenic hyper-tolerant bacterium Microbacterium paraoxydans strain IR-1 to determine its tolerance and toxicity to increasing doses of fluoride, either individually or in combination with arsenic, in terms of growth inhibition using a toxicity unit model. The minimum inhibitory concentration (MIC)and half maximal inhibitory concentration (IC50) values for fluoride increased, from 9 g/L to 11 g/L and from 5.91 ± 0.1 g/L to 6.32 ± 0.028 g/L, respectively, in the combination (F + As) group. The statistical comparison of observed and expected additive toxicities, with...
Prebiotics, Probiotics and Nutraceuticals, 2022
Therapeutic Implications of Natural Bioactive Compounds
Thousands of bioactive components are derived from various marine macro and microalgae. Such bene... more Thousands of bioactive components are derived from various marine macro and microalgae. Such beneficial algae are considered as a renewable and sustainable resource of bioactives with potential use as dietary food supplement, anti-viral, antiinflammatory, anti-cancerous, anti-oxidant, anti-diabetic, anti-bacterial agents which can provide nutritive and health care benefits. The biochemical infrastructure of algae comprises proteins, lipids, polysaccharides, minerals, vitamins etc. which can be used as nutritional and dietary sources along with use in therapeutics and cosmetics. The therapeutic and industrial applications of the algal derivatives are primarily due to the secondary metabolites such as astaxanthin, aquamin, alginates, fucoidan, omega-3- fatty acids, polyphenols, fucoxanthin, etc. This chapter focuses on various algae derived bioactives and their wide range of applications.
Therapeutic Implications of Natural Bioactive Compounds
The evolution of novel strategies for application in all facets of cancer theragnostic has shown ... more The evolution of novel strategies for application in all facets of cancer theragnostic has shown great progress in the past several decades. Bioactive compounds collected from plants and marine natural provenances have now been accredited as the crucial stepping stone to endow with fortification approach against several relentless ailments counting cancer. As per sundry investigations reports, the naturally occurring bioactive compounds possess an unprecedented molecular diversity with the potential to modulate several metabolic processes with high priority objectives such as low toxicity, targeting multiple drug resistance and heterogeneity of the tumor cells. These attributes with bioactive compounds can provide safe and high quality of healthy life achievable with easily available low-cost alternatives and nominal or no side effects. In topical quondam, numerous potent phytochemicals and marine molecules have been isolated, exemplified, identified, and are under disparate phases ...
Therapeutic Implications of Natural Bioactive Compounds
The COVID-19 pandemic has created a very difficult time for the whole world with utmost challenge... more The COVID-19 pandemic has created a very difficult time for the whole world with utmost challenge and responsibility on the public health sector to provide symptomatic relief and timely treatment. This condition has stimulated immediate research on the mode of transmission and pathogenesis of the viral variants. As the composition of SARS CoV-2 is similar to SARS-CoV by more than 50%, therefore the management of COVID-19 can possibly be done by repurposing the existing drugs used to treat SARS-CoV infection. The management of SARS CoV-2 infection can be performed at the level of control, prophylaxis and treatment. Many bioactive compounds isolated from medicinal plants have been studied for efficacy in controlling COVID-19 infection by either repression of viral host cell adjunction and subsequent penetration or by repression of viral genomic replication. The bioactive compounds target specific viral or host cell molecules in order to control the spread of virus. Another prominent a...
Therapeutic Implications of Natural Bioactive Compounds
The rising of chronic ailments impinged on humans worldwide has paved the urgent need for newer t... more The rising of chronic ailments impinged on humans worldwide has paved the urgent need for newer therapeutic vital compounds that are biologically active and have the capacity to endeavour without exerting any adverse or cytotoxic effects. This necessitates extensive research to investigate unexplored natural sources for such promising sources. A diverse array of fungi species has garnered considerable attention over the past century due to the assortment of their opportunities to generate novel active ingredients with multifunction mechanisms towards recuperative applications. Some of the fungal bioactive compounds possess exclusive therapeutic potential and pharmaceutical importance. These efficacious bioactive compounds including Paclitaxel, Podophyllotoxin, Enniatins, Camptothecin, Ascophytatin with their properties like anti-bacterial, antiviral, anti-parasitic, anti-diabetic, anti-cancerous, immunomodulatory are discussed in this chapter.
Therapeutic Implications of Natural Bioactive Compounds
The pure or standardized extracts obtained from microbes like bacteria, fungi, actinomycetes, etc... more The pure or standardized extracts obtained from microbes like bacteria, fungi, actinomycetes, etc. are considered important sources of bioactive compounds. Some of the microbes show inhibitory action against the growth of certain bacteria, fungi, yeast, insects, etc. This provides opportunities for the development of newer drugs and enzymes or beneficial compounds. Large number of bacteria are responsible for producing different bioactive compounds like antibiotics, enzymes, and other secondary metabolites. Some compounds like Gallic acid, Amicoumacin, Prodigiosin, Nystatin, Spinosad, Milbemycin, Lipstatin, Subtilin, Albaflavenone, and Mollemycin A have been studied for their affectivity against bacterial, fungal, insects, pests, etc. These compounds are gaining increasing interest because of their unique composition and the possibility of wide industrial applications.
Annals of Agri Bio Research, 2016
International Journal of Research in Pharmaceutical Sciences, 2013
Naturally occurring dietary components like Mentha piperita offer opportunities for development a... more Naturally occurring dietary components like Mentha piperita offer opportunities for development as effective radioprotective agents because of their potential low toxicity and therapeutic actions. The present investigation is an attempt to evaluate the radioprotective effect of Mentha piperita (Linn.) leaves on small intestine of Swiss albino mice exposed to gamma radiation. Mice were administered the optimum dose (100mg/kg/day) of 50% alcoholic extract of Mentha piperita (ALM) for three consecutive days prior to 6,8 ,10 Gy irradiation and autopsied at 1,3,7,14 and 30 day post irradiation to observe the histopathological changes in small intestine. The control group (irradiation alone), drug alone (ALM treatment at optimum dose) and normal group were run simultaneously. The results of histopathological studies did not show any changes in the normal histological structure of small intestine in the normal (Group I) and drug alone (Group II) at all the autopsy intervals. The control gr...
International journal of pharma and bio sciences, 2012
Natural Products offer an alternative to synthetic radioprotectors being less toxic. In this stud... more Natural Products offer an alternative to synthetic radioprotectors being less toxic. In this study 50% alcoholic extract of Mentha piperita (ALM) was tested for its radioprotective ability in terms of radiation sickness and survival of Swiss albino mice. This drug was well tolerated at highest dose of 1000mg/kg b.wt./day for three consecutive days. All doses of drug provided significant protection against radiation sickness and mortality, however the highest protection was observed at 100mg/kg. The optimum dose of 100mg/kg b.wt./day was determined on the basis of maximum survival and biochemical analysis of lowest lipid peroxidation and highest reduced glutathione levels. To determine the dose reduction factor (DRF) the optimum dose was administered for three consecutive days before 6,8,10 Gy gamma irradiation and 30 day survival was studied simultaneously with radiation sickness. The LD50/30 value of experimental (ALM + irradiation) was found to be considerably higher (8.052 ±0.115...
FLORA AND FAUNA
Metals and especially heavy metals are one of the major constituents of environmental pollution. ... more Metals and especially heavy metals are one of the major constituents of environmental pollution. The sources of heavy metal pollution can be geogenic or anthropogenic. The man-made sources include fumes, metal laden dust or food components. Although, many metals are essential for animal metabolism but a dose exceeding the required concentration is often deleterious. The hazardous nature of these heavy metals has been extensively studied in human, animal and plants showing various toxicological impacts of toxic metals. The acute or chronic exposure of heavy metals to animals and human can result in severe injury to the structure and function of vital organs as liver, kidney, brain along with systemic disease conditions. The growth of plants is also severely affected by the intake of metallic compounds through the mineral and water absorption route. The deleterious effects on plants range from symptoms of injury to metabolic, enzymatic, and growth inhibition. The study of heavy metal ...
In this study, we performed ecotoxicity tests of sodium arsenite on soil invertebrate Eisenia fet... more In this study, we performed ecotoxicity tests of sodium arsenite on soil invertebrate Eisenia fetida. Initially, broad range of sodium arsenite (0.1, 1, 10, 100mg/ml) was tested for mortality of E. fetida after 24 hours in filter paper test. The narrow range doses (0-1mg/ml) were further tested to determine the EC50 and LD50. The EC50 dose of sodium arsenite was considered at which 50% of earthworms exhibited rejection to the spot on filter paper in the spot rejection test. The LD50 of sodium arsenite resulting in mortality in 50% of Eisenia fetida was studied with contact filter paper bioassay (24hrs) and soil bioassay (14 days). In the range finding test, there was no mortality in control and 0.1mg/ml but 100% mortality was observed at doses of 1mg and above after 24 hours. Thus, for further study smaller doses intervals in the range between 0-1mg/ml was selected for spot rejection, contact filter paper and soil bioassay. The percent mortality in each test was computed and was sub...
Diagnostic Microbiology and Infectious Disease, 2015
Escherichia coli O157:H7 is a zoonotic pathogen with its ability to cause human illness ranging f... more Escherichia coli O157:H7 is a zoonotic pathogen with its ability to cause human illness ranging from diarrheal disease to fatal hemolytic uremic syndrome. E. coli O157:H7 had been associated with waterborne outbreaks resulting in high morbidity and mortality worldwide. Therefore, it is important to investigate the prevalence of E. coli O157:H7 in water sources especially used for drinking and to develop the diagnostic methods for its early detection. The review describes traditional cultural methods, immunological techniques, and polymerase chain reaction (PCR)-based methods for detection of this bacterium in water sources. The current PCR-based techniques such as real-time PCR are more specific and sensitive and require less detection time (<3 hours). These methods can be applied for regular water monitoring and proper management of water sources to prevent waterborne diseases due to E. coli O157:H7.
Water Quality, Exposure and Health, 2009
... Contaminated Water Harshneet Kaur · Deepshikha Mishra · Pradeep Bhatnagar · Pallavi Kaushik ·... more ... Contaminated Water Harshneet Kaur · Deepshikha Mishra · Pradeep Bhatnagar · Pallavi Kaushik · Swaran JS Flora Received: 27 March 2009 / Revised: 2 June 2009 / Accepted: 3 June 2009 / Published online: 17 July 2009 © Springer Science+Business Media BV 2009 ...
Oxidants and Antioxidants in Medical Science, 2013
The aim of this study is to analyze the potential hepatoprotective efficacy of alcoholic extract ... more The aim of this study is to analyze the potential hepatoprotective efficacy of alcoholic extract of Mentha piperita (Linn) against radiation-induced liver injury in Swiss albino mice after whole body irradiation in terms of histopathological lesions and levels of lipid peroxidation and reduced glutathione in liver tissue. M.piperita leaves were extracted in 50% ethanol and administered at the dose of 100 mg/kg b.wt/day for three consecutive days before whole body irradiation (6, 8 and 10 Gy). The extract provided significant hepatoprotection against different doses of radiation in terms of higher number of normal hepatocyte count, lower abnormal hepatocyte count and lower binucleated cell population along with other radiolesions as compared to irradiation alone group. The optimum dose of 100 mg/kg b.wt/day for three consecutive days also arrested decline in the level of reduced glutathione and elevation in lipid peroxidation significantly at autopsy intervals of 1, 3, 7, 14 and 30 days post-irradiation (6 and 8 Gy). The results of this study confirmed the hepatoprotective potential of M.piperita leaves alcoholic extract in controlling the development of radiolesions and number of abnormal hepatocytes in liver. This protection can be attributed to the antioxidant and antiperoxidant properties of Mentha piperita.
Asian Pacific journal of cancer prevention : APJCP
We report the chemopreventive activity of Acacia nilotica (Linn.) gum, flower and leaf aqueous ex... more We report the chemopreventive activity of Acacia nilotica (Linn.) gum, flower and leaf aqueous extracts, on 7,12-dimethylbenz(a)anthracene (DMBA) induced skin papillomagenesis in male Swiss albino mice. Animals were divided into following groups: Group I (Controls) given DMBA and croton oil, with no extract ; Group II (treatment) animals treated with Acacia nilotica gum (Group II-a) (800 mg/kg body weight), flowers (Group II-b) (800 mg/kg body weight), or leaves (Group II-c) (800 mg/kg body weight) during the peri- and post initiation periods of DMBA and croton oil application. A significant reduction in the values of tumor burden, tumor incidence and cumulative number of papillomas was observed in mice treated by oral gavage with the Acacia nilotica gum, flower and leaf extracts as compared with the control group. The latency period in treatment Group-II (b) and Group-II (c) was significantly increased as compared with the control group. A significant reduction in the frequency of ...
Toxicology International, 2012
Arsenic-contaminated areas of Sanganer, Jaipur, Rajasthan, India were surveyed for the presence o... more Arsenic-contaminated areas of Sanganer, Jaipur, Rajasthan, India were surveyed for the presence of metal resistant bacteria contaminated with textile effluent. Samples were collected from soil receiving regular effluent from the textile industries located at Sanganer area. The properties like pH, electrical conductivity, organic carbon, organic matter, exchangeable calcium, water holding capacity and metals like arsenic, iron, magnesium, lead and zinc were estimated in the contaminated soil. In total, nine bacterial strains were isolated which exhibited minimum inhibitory concentration (MIC) of arsenic ranging between 23.09 and 69.2mM. Four out of nine arsenic contaminated soil samples exhibited the presence of arsenite hyper-tolerant bacteria. Four high arsenite tolerant bacteria were characterized by 16S rDNA gene sequencing which revealed their similarity to Microbacterium paraoxydans strain 3109, Microbacterium paraoxydans strain CF36, Microbacterium sp. CQ0110Y, Microbacterium sp. GE1017. The above results were confirmed as per Bergey's Manual of Determinative Bacteriology. All the four Microbacterium strains were found to be resistant to 100μg/ml concentration of cobalt, nickel, zinc, chromium selenium and stannous and also exhibited variable sensitivity to mercury, cadmium, lead and antimony. These results indicate that the arsenic polluted soil harbors arsenite hyper-tolerant bacteria like Microbacterium which might play a role in bioremediation of the soil.
Article History: The industrial and technological progress for economic benefits has been imposin... more Article History: The industrial and technological progress for economic benefits has been imposing negative impact on the environment, in terms of its degradation and pollution. To find the effect of the industrial effluent on agricultural soil this study was conducted on impacted and non -impacted soil of two areas i.e. Sanganer and Durgapura respectively of Jaipur district. The soil quality was analyzed by estimation of physicochemical parameters such as pH, electrical conductivity(EC), water holding capacity, texture analysis, organic carbon, organic matter, total hardness, sodium, potassium concentration, sodium adsorption ratio(SAR), cation exchange capacity(CEC) using standard protocols . The results showed a significant difference between pH, EC, Water holding capacity, total hardness, SAR, CEC of both the soil, inferring the impact of industrial effluent on the quality of soil. Thus, to protect the deterioration soil quality, control on such industrial pollution assumes grea...
Uploads
Papers by Dr.Pallavi Kaushik