Open Access Macedonian Journal of Medical Sciences, 2014
Citation: Elsharkawy SL, AbdEl-Aal WE, Esmal RSE, Ali HHM, Mahfouz SM, El-Habashi A. Preoperative... more Citation: Elsharkawy SL, AbdEl-Aal WE, Esmal RSE, Ali HHM, Mahfouz SM, El-Habashi A. Preoperative Evaluation of Thyroid Epithelial Lesions by DNA Ploidy and Galectin-3 Expression in FNAC. Maced J Med Sci. 2014 Dec 15; 7(4):585-594. http://dx.
Thirty-nine primary synovial sarcomas (15 biphasic, 24 monophasic), and 19 metastatic synovial sa... more Thirty-nine primary synovial sarcomas (15 biphasic, 24 monophasic), and 19 metastatic synovial sarcomas were studied with a battery of antibodies directed to keratin, epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), vimentin, desmin, muscle-specific actin, smooth muscle actin, S-100 protein, Leu-7, chromogranin A, laminin, collagen IV, Ulex europaeus agglutinin I (UEAI), and the HMB-45 antimelanoma antibody. Twenty-two primary and 18 metastatic synovial sarcomas were also examined by electron microscopy. Epithelial and/or spindle cells in every biphasic tumor, primary and metastatic, reacted for keratin and EMA, but only six primary tumors (five biphasic and one monophasic) showed weak reactivity for CEA which, in the biphasic tumors, was confined to the epithelial component. Of the monophasic tumors, 15 primary (63%) and four metastatic (25%) stained for keratin, whereas seven primary (29%) and two metastatic (13%) tumors reacted for EMA. Only one primary monophasic synovial sarcoma stained for CEA. Tumors that stained for EMA or CEA also stained for keratin which is, therefore, the most useful epithelial marker. Immunostaining for epithelial markers, UEAI, collagen IV, and laminin serves to delineate the epithelial component when it is obscure in routine sections. Electron microscopy facilitates the diagnosis when epithelial markers are not expressed and aids in separating monophasic synovial sarcomas from other sarcomas that they resemble by light microscopy.
ABSTRACT Background: Breast cancer is the most common form of cancer among Egyptian women. Aim of... more ABSTRACT Background: Breast cancer is the most common form of cancer among Egyptian women. Aim of the work: Molecules such as CD1a and matrix metalloproteinase-9 (MMP-9), involved in tumor invasion and spread, are candidates that have been identified as new prognostic markers. Material and methods: The current study included 101 patients with invasive breast carcinoma, representing both metastatic and nonmetastatic groups. Results: Tumors were studied for infiltration by CD1a-positive dendritic cells and for expression of MMP-9. No statistically significant correlation was found between the former two variables and the metastatic potential of the tumor. Conclusion: CD1a and MMP-9 cannot, therefore, be considered as independent.
Background: Laminins are components of the extracellular matrix that mediate cell adhesion, growt... more Background: Laminins are components of the extracellular matrix that mediate cell adhesion, growth, migration, proliferation and differentiation. Basement membrane (BM) laminins, in particular, may play a role in enhancing carcinoma cell motility. Aim: To evaluate the distribution pattern of laminin in basal cell carcinoma (BCC), as regards the basement membrane, cellular cytoplasm, peritumoral lacunae and surface epithelium and to correlate laminin distribution with different variants of BCC. Patients and Methods: Skin biopsy specimens were obtained from 21 BCC patients for routine histopathological and immunohistochemical study. Laminin was evaluated qualitatively and semiquantitatively using monoclonal mouse antihuman antibody (Dako-Laminin, 4C7. Code No: MO638, which reacts with the terminal globular domain of the α5 chain) Results: The majority of BCC cases showed patchy cytoplasmic distribution of laminin. The BM expression of laminin, in most cases, was well defined, fine and linear with irregular areas of thickening. Staining intensity was moderate in differentiated and mixed variants, weak in superficial spreading and absent in morpheic types. Conclusion: Cytoplasmic and basement membrane laminin is important in the pathogenesis and invasion of BCC. Most laminin was in basement membrane zone (BMZ), and the more differentiated the tumor, the more cytoplasmic and BM staining it expressed.
Background. Papular urticaria is a chronic allergic disease characterized by selective accumulati... more Background. Papular urticaria is a chronic allergic disease characterized by selective accumulation of eosinophils. Eotaxin is a chemokine that is a potent and selective chemoattractant for eosinophils both in vivo and in vitro. To date, eotaxin has not been studied in papular urticaria. Objective. To detect eotaxin, semiquantitatively, in lesional skin of patients with papular urticaria and to investigate the presence of any correlation between eotaxin density and the various demographic, clinical, and histopathological features of the disease. Patients and methods. The study included 29 patients with papular urticaria and 32 control subjects. Immunohistochemical study of the amount and distribution pattern of eotaxin was performed using eotaxin (C-19) polyclonal antibody (IgG). Results. Eotaxin expression was more significantly evident in the patient group than in controls (p < 0.001) and appeared to be positively correlated to the inflammatory cell population. Epidermal eotaxin distribution pattern was absent or patchy in the patient group whereas in healthy controls the predominant pattern was linear and continuous along the basal cell layer. A predominant dermal eotaxin distribution, mostly in interstitial and perivascular compartments, was observed in the patient group. Lesion severity did not correlate statistically with eotaxin expression; neither did the patient age, sex, disease duration, recurrence rate, clinical features or histopathological patterns. Conclusion. Eotaxin expression is deranged in epidermis and upregulated in the dermis of patients with papular urticaria compared with healthy controls. These findings may represent a possible mechanism for promoting tissue inflammation in this disease which needs further research. (J Egypt Women Dermatol Soc 2010; 7: 134 -140)
Journal of Clinical and Experimental Hepatology, 2015
Background/objective: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors w... more Background/objective: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. The highest prevalence of hepatitis is an important risk factor contributing to development of HCCs. However, an increasing number of cases are associated metabolic disease and steatohepatitis. Inflammation associated with many liver disease, seems to be a necessary pre-requisite for successful tumor initiation. Mechanisms that link high fat diet and inflammation initial stage of HCC are not completely understood.
Tumors of the central nervous system (CNS) represent a unique, heterogeneous population of neopla... more Tumors of the central nervous system (CNS) represent a unique, heterogeneous population of neoplasms and include both benign and malignant tumors. The present study was carried out on a total of 79 archival cases of ependymal tumors in addition to a variety of other primary CNS tumors. The study entailed the use of CD99 monoclonal antibody and epithelial membrane antigen (EMA). It was found that all 38 ependymoma cases (classic and nonclassic) showed positive membranous and intracytoplasmic CD99 immunoreactivity. Upon comparing with other CNS tumors (41 cases), it was found that CD99 could differentiate between ependymomas and nonependymal tumors, but intensity and pattern of staining were of no consequence in determining variant type or degree of histologic aggressiveness. In regard to EMA immunoreactivity, which was restricted to the ependymoma group, 2 patterns of staining could be detected--the intracytoplasmic dotlike pattern and the ringlike pattern--but some cases were comple...
Chronic renal failure (CRF) is a common disease with high morbidity and mortality. The inadequacy... more Chronic renal failure (CRF) is a common disease with high morbidity and mortality. The inadequacy of current treatment modalities and insufficiency of donor organs for cadaveric transplantation have driven a search for improved methods of dealing with renal failure. This raises the concept of cell-based therapy using mesenchymal stem cells (MSCs). The purpose of this work is to evaluate the effect of MSCs in rats with CRF and to detect the level of B-cell lymphoma 2 (Bcl-2) and basic fibroblast growth factor (bFGF) as possible antiapoptotic and anti-inflammatory paracrine mediators of MSCs action. This study was carried on female albino rats, which were divided into 3 groups, control group, CRF group and CRF/MSCs group. MSCs were derived from bone marrow of male albino rats and were characterized morphologically and by RT-PCR for CD29. Serum urea, creatinine, Na, K, systolic blood pressure and 24-hour urinary albumin were estimated for all groups, Y-chromosome gene (Sry) was detected by PCR in renal tissue. Also Bcl-2 and bFGF were examined by RT-PCR in renal tissue of all studied groups and the kidney was examined pathologically. The results of this work showed that CRF rats receiving MSCs showed significantly lowered serum urea, creatinine and urinary albumin levels compared to the CRF group. Also improvement of serum Na and K was observed in CRF/MSCs group compared to CRF group. As regard Bcl-2 and bFGF they were highly expressed in renal tissue of CRF/MSCs group compared to the CRF group. The (Sry) gene was detected by PCR in the renal tissue of CRF/MSCs group. These results demonstrate a potential of MSCs to ameliorate the kidney function in rats with CRF possibly through the antiapoptotic and anti-inflammatory paracrine mediators of MSCs.
To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in... more To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in a retrospective study. Bilharzial bladder carcinoma is the most common cancer, particularly in Egyptian men. Classically, carcinoma in a bilharzial bladder is most commonly of the squamous cell type. During the past decade, certain changes have occurred in the features in Schistosomiasis-associated carcinoma in Egypt with a decline in the frequency of squamous cell carcinoma and increase in the frequency of transitional cell carcinoma.
To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in... more To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in a retrospective study. Bilharzial bladder carcinoma is the most common cancer, particularly in Egyptian men. Classically, carcinoma in a bilharzial bladder is most commonly of the squamous cell type. During the past decade, certain changes have occurred in the features in Schistosomiasis-associated carcinoma in Egypt with a decline in the frequency of squamous cell carcinoma and increase in the frequency of transitional cell carcinoma.
Open Access Macedonian Journal of Medical Sciences, 2014
Citation: Elsharkawy SL, AbdEl-Aal WE, Esmal RSE, Ali HHM, Mahfouz SM, El-Habashi A. Preoperative... more Citation: Elsharkawy SL, AbdEl-Aal WE, Esmal RSE, Ali HHM, Mahfouz SM, El-Habashi A. Preoperative Evaluation of Thyroid Epithelial Lesions by DNA Ploidy and Galectin-3 Expression in FNAC. Maced J Med Sci. 2014 Dec 15; 7(4):585-594. http://dx.
Thirty-nine primary synovial sarcomas (15 biphasic, 24 monophasic), and 19 metastatic synovial sa... more Thirty-nine primary synovial sarcomas (15 biphasic, 24 monophasic), and 19 metastatic synovial sarcomas were studied with a battery of antibodies directed to keratin, epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), vimentin, desmin, muscle-specific actin, smooth muscle actin, S-100 protein, Leu-7, chromogranin A, laminin, collagen IV, Ulex europaeus agglutinin I (UEAI), and the HMB-45 antimelanoma antibody. Twenty-two primary and 18 metastatic synovial sarcomas were also examined by electron microscopy. Epithelial and/or spindle cells in every biphasic tumor, primary and metastatic, reacted for keratin and EMA, but only six primary tumors (five biphasic and one monophasic) showed weak reactivity for CEA which, in the biphasic tumors, was confined to the epithelial component. Of the monophasic tumors, 15 primary (63%) and four metastatic (25%) stained for keratin, whereas seven primary (29%) and two metastatic (13%) tumors reacted for EMA. Only one primary monophasic synovial sarcoma stained for CEA. Tumors that stained for EMA or CEA also stained for keratin which is, therefore, the most useful epithelial marker. Immunostaining for epithelial markers, UEAI, collagen IV, and laminin serves to delineate the epithelial component when it is obscure in routine sections. Electron microscopy facilitates the diagnosis when epithelial markers are not expressed and aids in separating monophasic synovial sarcomas from other sarcomas that they resemble by light microscopy.
ABSTRACT Background: Breast cancer is the most common form of cancer among Egyptian women. Aim of... more ABSTRACT Background: Breast cancer is the most common form of cancer among Egyptian women. Aim of the work: Molecules such as CD1a and matrix metalloproteinase-9 (MMP-9), involved in tumor invasion and spread, are candidates that have been identified as new prognostic markers. Material and methods: The current study included 101 patients with invasive breast carcinoma, representing both metastatic and nonmetastatic groups. Results: Tumors were studied for infiltration by CD1a-positive dendritic cells and for expression of MMP-9. No statistically significant correlation was found between the former two variables and the metastatic potential of the tumor. Conclusion: CD1a and MMP-9 cannot, therefore, be considered as independent.
Background: Laminins are components of the extracellular matrix that mediate cell adhesion, growt... more Background: Laminins are components of the extracellular matrix that mediate cell adhesion, growth, migration, proliferation and differentiation. Basement membrane (BM) laminins, in particular, may play a role in enhancing carcinoma cell motility. Aim: To evaluate the distribution pattern of laminin in basal cell carcinoma (BCC), as regards the basement membrane, cellular cytoplasm, peritumoral lacunae and surface epithelium and to correlate laminin distribution with different variants of BCC. Patients and Methods: Skin biopsy specimens were obtained from 21 BCC patients for routine histopathological and immunohistochemical study. Laminin was evaluated qualitatively and semiquantitatively using monoclonal mouse antihuman antibody (Dako-Laminin, 4C7. Code No: MO638, which reacts with the terminal globular domain of the α5 chain) Results: The majority of BCC cases showed patchy cytoplasmic distribution of laminin. The BM expression of laminin, in most cases, was well defined, fine and linear with irregular areas of thickening. Staining intensity was moderate in differentiated and mixed variants, weak in superficial spreading and absent in morpheic types. Conclusion: Cytoplasmic and basement membrane laminin is important in the pathogenesis and invasion of BCC. Most laminin was in basement membrane zone (BMZ), and the more differentiated the tumor, the more cytoplasmic and BM staining it expressed.
Background. Papular urticaria is a chronic allergic disease characterized by selective accumulati... more Background. Papular urticaria is a chronic allergic disease characterized by selective accumulation of eosinophils. Eotaxin is a chemokine that is a potent and selective chemoattractant for eosinophils both in vivo and in vitro. To date, eotaxin has not been studied in papular urticaria. Objective. To detect eotaxin, semiquantitatively, in lesional skin of patients with papular urticaria and to investigate the presence of any correlation between eotaxin density and the various demographic, clinical, and histopathological features of the disease. Patients and methods. The study included 29 patients with papular urticaria and 32 control subjects. Immunohistochemical study of the amount and distribution pattern of eotaxin was performed using eotaxin (C-19) polyclonal antibody (IgG). Results. Eotaxin expression was more significantly evident in the patient group than in controls (p < 0.001) and appeared to be positively correlated to the inflammatory cell population. Epidermal eotaxin distribution pattern was absent or patchy in the patient group whereas in healthy controls the predominant pattern was linear and continuous along the basal cell layer. A predominant dermal eotaxin distribution, mostly in interstitial and perivascular compartments, was observed in the patient group. Lesion severity did not correlate statistically with eotaxin expression; neither did the patient age, sex, disease duration, recurrence rate, clinical features or histopathological patterns. Conclusion. Eotaxin expression is deranged in epidermis and upregulated in the dermis of patients with papular urticaria compared with healthy controls. These findings may represent a possible mechanism for promoting tissue inflammation in this disease which needs further research. (J Egypt Women Dermatol Soc 2010; 7: 134 -140)
Journal of Clinical and Experimental Hepatology, 2015
Background/objective: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors w... more Background/objective: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. The highest prevalence of hepatitis is an important risk factor contributing to development of HCCs. However, an increasing number of cases are associated metabolic disease and steatohepatitis. Inflammation associated with many liver disease, seems to be a necessary pre-requisite for successful tumor initiation. Mechanisms that link high fat diet and inflammation initial stage of HCC are not completely understood.
Tumors of the central nervous system (CNS) represent a unique, heterogeneous population of neopla... more Tumors of the central nervous system (CNS) represent a unique, heterogeneous population of neoplasms and include both benign and malignant tumors. The present study was carried out on a total of 79 archival cases of ependymal tumors in addition to a variety of other primary CNS tumors. The study entailed the use of CD99 monoclonal antibody and epithelial membrane antigen (EMA). It was found that all 38 ependymoma cases (classic and nonclassic) showed positive membranous and intracytoplasmic CD99 immunoreactivity. Upon comparing with other CNS tumors (41 cases), it was found that CD99 could differentiate between ependymomas and nonependymal tumors, but intensity and pattern of staining were of no consequence in determining variant type or degree of histologic aggressiveness. In regard to EMA immunoreactivity, which was restricted to the ependymoma group, 2 patterns of staining could be detected--the intracytoplasmic dotlike pattern and the ringlike pattern--but some cases were comple...
Chronic renal failure (CRF) is a common disease with high morbidity and mortality. The inadequacy... more Chronic renal failure (CRF) is a common disease with high morbidity and mortality. The inadequacy of current treatment modalities and insufficiency of donor organs for cadaveric transplantation have driven a search for improved methods of dealing with renal failure. This raises the concept of cell-based therapy using mesenchymal stem cells (MSCs). The purpose of this work is to evaluate the effect of MSCs in rats with CRF and to detect the level of B-cell lymphoma 2 (Bcl-2) and basic fibroblast growth factor (bFGF) as possible antiapoptotic and anti-inflammatory paracrine mediators of MSCs action. This study was carried on female albino rats, which were divided into 3 groups, control group, CRF group and CRF/MSCs group. MSCs were derived from bone marrow of male albino rats and were characterized morphologically and by RT-PCR for CD29. Serum urea, creatinine, Na, K, systolic blood pressure and 24-hour urinary albumin were estimated for all groups, Y-chromosome gene (Sry) was detected by PCR in renal tissue. Also Bcl-2 and bFGF were examined by RT-PCR in renal tissue of all studied groups and the kidney was examined pathologically. The results of this work showed that CRF rats receiving MSCs showed significantly lowered serum urea, creatinine and urinary albumin levels compared to the CRF group. Also improvement of serum Na and K was observed in CRF/MSCs group compared to CRF group. As regard Bcl-2 and bFGF they were highly expressed in renal tissue of CRF/MSCs group compared to the CRF group. The (Sry) gene was detected by PCR in the renal tissue of CRF/MSCs group. These results demonstrate a potential of MSCs to ameliorate the kidney function in rats with CRF possibly through the antiapoptotic and anti-inflammatory paracrine mediators of MSCs.
To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in... more To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in a retrospective study. Bilharzial bladder carcinoma is the most common cancer, particularly in Egyptian men. Classically, carcinoma in a bilharzial bladder is most commonly of the squamous cell type. During the past decade, certain changes have occurred in the features in Schistosomiasis-associated carcinoma in Egypt with a decline in the frequency of squamous cell carcinoma and increase in the frequency of transitional cell carcinoma.
To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in... more To assess the patterns of schistosomiasis-associated bladder cancer in Egypt from 2001 to 2010 in a retrospective study. Bilharzial bladder carcinoma is the most common cancer, particularly in Egyptian men. Classically, carcinoma in a bilharzial bladder is most commonly of the squamous cell type. During the past decade, certain changes have occurred in the features in Schistosomiasis-associated carcinoma in Egypt with a decline in the frequency of squamous cell carcinoma and increase in the frequency of transitional cell carcinoma.
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