Papers by Sattwikesh Paul
Objective: To establish ultrasound guided testicular fine needle aspiration (TFNA) as well as to ... more Objective: To establish ultrasound guided testicular fine needle aspiration (TFNA) as well as to assess the effectiveness of uni-directional (UD) and multi-directional (MD) TFNA in buck according to testicular cells, echotexture and gross changes of testicle, age of buck
was considered. Methods: A total of 120 samples were collected with both directions (UD,
n=60) and (MD, n=60) suction from testis of 10 apparently healthy bucks. All slides were stained with May-Grünwald-Giemsa and examined under light microscope with 1 000 × magnifications to count spermatogenic cells, spermatozoa and sertoli cells. The percentage of
spermatozoa and sertoli cells were expressed as spermatic index and sertoli cell index. Results: Results revealed no difference in the presence of various spermatogenic and sertoli cells in cell cluster of slides made either unidirectional TFNA or multidirectional TFNA. Early spermatids
were the most numerous, followed by late spermatids, primary spermatocytes, spermatogonia. Sertoli cell index was higher in TFNA smears of young bucks prepared from (7–13 months) of age and spermatic index was higher in adult bucks (14–24 months) of age. No echogenic change was observed in the echotexture of testisafter TFNA. Conclusion: It seems that TFNA has no serious ill effect on the buck testis when uni-direction aspiration is performed. Moreover, the possibility to standardize this method might provide a greater impulse to the clinical diagnostics of male animal infertility.
Congenital urinary tract obstruction is a developmental anomaly which may persist after birth in ... more Congenital urinary tract obstruction is a developmental anomaly which may persist after birth in new born calves. To determine the occurrence of congenital urinary obstruction and to evaluate tube cystostomy for the management of congenital urinary obstruction in neonatal calves two studies were conducted. A retrospective study was performed to observe the occurrence of congenital urinary obstruction in neonatal ruminants from January 2005 to November 2014. Occurrence of congenital urinary obstruction in neonatal calves was also studied in respect to age, sex, breed and season. During the period of 10 years, a total of 42 cases of congenital urinary obstruction were recorded, which constituted an incidence of 2.0%. In this study, maximum incidence was recorded in bovine is 38 (90%). Maximum number of cases (45%) of congenital urinary obstruction in ruminants was recorded from March to June, July to October and November to February as 19 (45%), 9 (22%) and 14 (33%) respectively. Among affected animals, 31 were cross breed (74%) and 11 were local breed (26%). Number of affected males was 29 (69%) clearly ahead than affected female 13 (31%). In another study, tube cystostomy with infusion set was performed in eleven neonatal calves aged 1-6 days old and observed for 1 month. Among 11 calves, 2 died due to severe infection and septicemia and 2 calves needed repeated operation. Seven calves got uneventful recovery and the cured animal showed satisfactory results. Overall successful rate was 63.6%. The results revealed that tube cystostomy seemed to be an effective method. In addition, this study will help not only veterinarian to make them aware about the occurrence of congenital urinary obstruction but also to solve the problem by saving the life of neonatal calves.
The myiasis is very common surgical affections of ruminants in Bangladesh. There are limited rese... more The myiasis is very common surgical affections of ruminants in Bangladesh. There are limited researches on the maggot wound infestation in Bangladesh. A retrospective study was performed to collect data and to get a clear and genuine concept on myiasis affections in ruminants in respect to species. age, sex, breed, season and predilection sites. The present study was conducted in Veterinary Clinic, Bangladesh Agricultural University, Mymensingh and data was recorded from 2008 to 2012. Results showed that 151 animals were affected with myiasis and maggot wounds predominantly occurred in the cattle (71.6%) among which calves were more affected. Myiasis was very prone to occur in the navel, vulva, leg and ear. During the study period, more affection occurred in animals of below 6 months (41%). The females (66%) were more frequently affected than the males (34%) and cross breed (57%) was more affected compared to local breed (43%).Moreover, infestation frequently observed from March to June (80%). This study could help veterinarians as well as farmers to be aware of the occurrence of maggot wounds and to implement protective measures with minimum complications.
Various reduction and fixation techniques have been introduced for the treatment of different typ... more Various reduction and fixation techniques have been introduced for the treatment of different types of fractures. Therefore, this study was planned to evaluate two methods of fracture treatments in calves having complete fractures due to automobile accident. In addition, a retrospective study was done to observe the occurrence of fractures in calves. Calves presented with the history of automobile accident and clinical signs suggestive of fractures were subjected to detailed physical, orthopaedic and radiographic examination to confirm fractures. For treatment 20 fractures case were included, where 8 cases were treated with closed reduction with external fixation using bamboo splints and plaster of paris. Whereas, open reduction with internal fixation using stainless steel orthopaedic wires and modified thomas splints were used to treat 12 fractures in long bones of calves aged 8 days to 5 months.All calves treated with open reduction and internal fixation showed good weight bearing in the immediate post-operative period. The fixators applied to different bones were well-tolerated, and the animals could lie down, stand and walk freely with the fixator without any problems after 60 days. Post operative radiography confirmed that bone fragments were well maintained until healing occurred and radiograph showed negligible callus and a radioluscent line (less remarkeble radiolucent area) at 60 days. There was no periosteal reaction around the wire suture at the fracture site. So, wire suture provided good alignment and stabilization of fracture fragments, rapid union of fracture resulting in early functional usage of limb. In contrast, two calves cured among 8 calves treated with closed reduction and external fixation, this study resulted that prognosis was not favorable in calves The results of this study exemplify the successful use of wire sutures in the treatment of complete fractures of the long bones in calves.
The study was conducted to evaluate homogenous platelets rich plasma (PRP) gel in the bone healin... more The study was conducted to evaluate homogenous platelets rich plasma (PRP) gel in the bone healing process. There are limited researches on the use of biomaterials to assist healing process in Bangladesh done earier. Therefore, this experiment was carried out to exploit the therapeutic effects of PRP gel on healing process. For bone healing study, total 6 oblique tibial fracture cases in goats were created. Goats were divided into three groups with two animals in each group bearing weight 15-20 kg and age 1-3 years. Among these two groups were treated with homogenous PRP gel and one group was untreated served as control and fracture cases were corrected with open reduction and internal fixation using bone plate with screw and surgical stainless steel wire (18 Gauze) suture. Goats showed a satisfactory result on bone healing after using PRP gel. Post operative radiography confirmed that bone fragments were well maintained until healing occurred and radiograph showed negligible callus at 30 days, complete healing occur. On the other hands, it takes 60 days for immobilization in untreated group. In treatment groups there was no periosteal reaction at the fracture site. No postoperative complication was observed at surgical site. This study could help veterinarians to consider natural biomaterial product specially homogenous PRP gel for a good healing of bone with minimum complications. Further studies are necessary for the molecular investigation of healing process and proper establishment of commercially available of PRP gel in our country and easyiest way of application.
The study was conducted to evaluate the efficiency of platelet rich plasma (PRP) gel in the heali... more The study was conducted to evaluate the efficiency of platelet rich plasma (PRP) gel in the healing of skin wound.This experiment was carried out to exploit the therapeutic effects of PRP gel on healing process. A total of 72 surgical wounds were madein 12 goatsbearing weight 15-20 kg with the age 1-3 years. Goats were divided into four groups with three animals in each group. Wounds of four groups were treated with homogenous PRP gel, heterogenousPRP gel, sulphonilamide powder and tincture benzoinCo. or benzoin seal (control). The wounds were 3 cm length and 0.5 cm depth sutured with cross mattress using nylon. Follow-up observation was recorded from day 1 to day 30 postoperatively. Some morphological characters such as swelling area of wound, elevation of sutured line from the skin surface, width of sutured area and contraction length between 7 to 15 days were recorded to determine the healing process of the wounds. Besides morphological study, histopathological studies were also performed on day 1, day 2, day 3, day 5 and day 15 to evaluate the healing process.Homogenous PRP gel showed admirable results in the healing of the wounds produced in goat. All wounds treated with homogenous PRP gel showed no inflammation with dry sutured area and complete healing with massive hair follicular growth within 15-21 days. On histopathological study, demonstrated epithelial tissues with a normal morphology. The dermis showed flabby connective tissue with organized interconnecting collagen fibers running parallel to each other and new hair follicular growth inhealed area within 15 days, when wounds were treated with homogenousPRP gel.This study could help to consider natural biomaterial product specially PRP gel homogenous for a good healing of skin wounds with minimum complications. Further studies are necessary for the molecular investigation of healing process and proper establishment of commercially available of PRP gel and easiest finding theway of it'sapplication.
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Papers by Sattwikesh Paul
was considered. Methods: A total of 120 samples were collected with both directions (UD,
n=60) and (MD, n=60) suction from testis of 10 apparently healthy bucks. All slides were stained with May-Grünwald-Giemsa and examined under light microscope with 1 000 × magnifications to count spermatogenic cells, spermatozoa and sertoli cells. The percentage of
spermatozoa and sertoli cells were expressed as spermatic index and sertoli cell index. Results: Results revealed no difference in the presence of various spermatogenic and sertoli cells in cell cluster of slides made either unidirectional TFNA or multidirectional TFNA. Early spermatids
were the most numerous, followed by late spermatids, primary spermatocytes, spermatogonia. Sertoli cell index was higher in TFNA smears of young bucks prepared from (7–13 months) of age and spermatic index was higher in adult bucks (14–24 months) of age. No echogenic change was observed in the echotexture of testisafter TFNA. Conclusion: It seems that TFNA has no serious ill effect on the buck testis when uni-direction aspiration is performed. Moreover, the possibility to standardize this method might provide a greater impulse to the clinical diagnostics of male animal infertility.
was considered. Methods: A total of 120 samples were collected with both directions (UD,
n=60) and (MD, n=60) suction from testis of 10 apparently healthy bucks. All slides were stained with May-Grünwald-Giemsa and examined under light microscope with 1 000 × magnifications to count spermatogenic cells, spermatozoa and sertoli cells. The percentage of
spermatozoa and sertoli cells were expressed as spermatic index and sertoli cell index. Results: Results revealed no difference in the presence of various spermatogenic and sertoli cells in cell cluster of slides made either unidirectional TFNA or multidirectional TFNA. Early spermatids
were the most numerous, followed by late spermatids, primary spermatocytes, spermatogonia. Sertoli cell index was higher in TFNA smears of young bucks prepared from (7–13 months) of age and spermatic index was higher in adult bucks (14–24 months) of age. No echogenic change was observed in the echotexture of testisafter TFNA. Conclusion: It seems that TFNA has no serious ill effect on the buck testis when uni-direction aspiration is performed. Moreover, the possibility to standardize this method might provide a greater impulse to the clinical diagnostics of male animal infertility.