Research article QTL for microstructural and biophysical muscle properties and body composition i... more Research article QTL for microstructural and biophysical muscle properties and body composition in pigs
The genetic variability of various local chicken populations derived from Bolivia, India, Nigeria... more The genetic variability of various local chicken populations derived from Bolivia, India, Nigeria and Tanzania was evaluated with 22 microsatellites. Between two and 11 alleles per locus were detected. All populations showed high levels of heterozygosity with the lowest value of 45% for the population named Aseel from India and the highest value of 67% for Arusha from Tanzania. A dendrogram was constructed based on CHORD distance by upgma analysis. Within this tree the populations were assorted according to their geographical origin. Bootstrapping values within the dendrogram were between 37 and 99%. The contribution of the determination of genetic variability with genetic markers to the decision on conservation and/or further use of the populations in crossbreeding programs designed to create genetic stocks with improved adaptability and productivity in tropical countries is discussed.
The identification of important genetic resources and the prevention of further loss of genetic v... more The identification of important genetic resources and the prevention of further loss of genetic variation is an important task. In developing countries selection pressure for production traits is low and traditional chicken breeds are used in rural animal production ...
Background: The proportion of muscle fibre types and their size affect muscularity as well as fun... more Background: The proportion of muscle fibre types and their size affect muscularity as well as functional properties of the musculature and meat quality. We aimed to identify QTL for microstructural muscle properties including muscle fibre size, their numbers and fibre type proportions as well as biophysical parameters of meat quality and traits related to body composition, i.e. pH, conductivity, area of M. longissimus dorsi and lean meat content. A QTL scan was conducted in a porcine experimental population that is based on Duroc and Berlin Miniature Pig. Results: Least square regression interval mapping revealed five significant and 42 suggestive QTL for traits related to muscle fibre composition under the line-cross model as well as eight significant and 40 suggestive QTL under the half-sib model. For traits related to body composition and biophysical parameters of meat quality five and twelve significant plus nine and 22 suggestive QTL were found under the line-cross and half-sib model, respectively. Regions with either significant QTL for muscle fibre traits or significant QTL for meat quality and muscularity or both were detected on SSC1, 2, 3, 4, 5, 13, 14, 15, and 16. QTL for microstructural properties explained a larger proportion of variance than did QTL for meat quality and body composition. Conclusion: Microstructural properties of pig muscle and meat quality are governed by genetic variation at many loci distributed throughout the genome. QTL analysis under both, the line-cross and half-sib model, allows detecting QTL in case of fixation or segregation of the QTL alleles among the founder populations and thus provide comprehensive insight into the genetic variation of the traits under investigation. Genomic regions affecting complex traits of muscularity and meat quality as well as microstructural properties might point to QTL that in first instance affect muscle fibre traits and by this in second instance meat quality. Disentangling complex traits in their constituent phenotypes might facilitate the identification of QTL and the elucidation of the pleiotropic nature of QTL effects.
In order to demonstrate the potential power of maternal effects on litter size and growth in pigs... more In order to demonstrate the potential power of maternal effects on litter size and growth in pigs a reciprocal cross of Berlin Miniature Pigs and Durocs was carried out. The average body weight of the Duroc dams (151 kg) was more than three times higher than the weight of the Miniature Pig dams (48 kg). A considerable maternal effect on fertility, estimated from reciprocal crossbred differences in the litter size of 3.5 piglets was found. The least Squares means of birth weight were 1.18 kg and 0.75 kg for Fl animals bom from Duroc dams and Miniature Pig dams, respectively. This maternal caused disadvantage in body weight of crossbred offspring from Miniature Pig dams is compensated in the interval from weaning to the 13th week of age. The end weight estimated by means of Gompertz and Bertalanffy growth functions showed only minor differences between the reciprocal crossed groups in favour of Fl born from Duroc dams. Our data suggest that the uterine capacity of the dam could play a...
Because of the importance of the complement system in disease resistance through direct lysis of ... more Because of the importance of the complement system in disease resistance through direct lysis of target cells and support of other mechanisms of the immune System, individual Variation in haemolytic complement activity and C3 concentration in pigs prior and after vaccination were evaluated. Possible effects on the variation of these parameters were investigated. Total complement activity was determined based on complement-mediated haemolysis of antibody-sensitised erythrocytes and C3c levels were measured by immunonephelometry in Gemian Landrace pigs and an experimental pig population. Both methods revealed remarkable individual differences. The arithmetic means of total haemolytic complement activity including all pigs were 42.3 ± 28.2 U/ml before vaccination and 59.1 ± 50.6 U/ml after three immunisations. Mean plasma concentrations of C3c were between .102 ± .035 g/1 before immunisation and .126 ± .038 g/1 on day 4 after Aujeszky vaccination. Existing p...
The genetic basis of body weight in the mouse was investigated by measuring frequency changes of ... more The genetic basis of body weight in the mouse was investigated by measuring frequency changes of microsatellite marker alleles in lines divergently selected for body weight from a base population of a cross between two inbred strains. In several regions of the genome, sharp peaks of frequency change at linked markers were detected, which suggested the presence of single genes of moderate effect, although in several other regions, significant frequency changes occurred over large portions of chromosomes. A method based on maximum likelihood was used to infer effects and map positions of quantitative trait loci (QTLs) based on genotype frequencies at one or more marker loci. Eleven QTLs with effects in the range 0.17-0.28 phenotypic standard deviations were detected; but under an additive model, these did not fully account for the observed selection response. Tests for the presence of more than one QTL in regions where there were large changes of marker allele frequency were mostly in...
In Longissimus muscle from a F 2 population of DurocÂBerlin Miniature Pigs, micro-structural fibr... more In Longissimus muscle from a F 2 population of DurocÂBerlin Miniature Pigs, micro-structural fibre traits and fatty acid composition were investigated to calculate correlation coefficients between these traits and meat quality. The animals of the F 2 population exhibited low carcass weight (55.7 AE11.2 kg), low meat percentage (35.0AE 8.4%) but a relatively high intramuscular fat content (3.52AE 1.44%) compared to pure bred animals (F 0 ). No unacceptable meat quality was observed. The variation coefficients of carcass composition, muscle fibre traits, and fat traits were high enough to allow the analysis of candidate genes which influence the growth of muscle fibres, fat cells, and meat quality. Phenotypic correlation coefficients between muscle fibre characteristics and meat quality traits were low whereas fatty acid composition and meat quality were more closely related. The correlation coefficients between muscle fibre traits and fatty acid composition ranged from 0.10 to 0.40. The relationship between a low quotient of n-6/n-3 fatty acids in muscle and greater fibre sizes, higher percentages of the oxidative fibre type and higher capillary density was noteworthy indicating good conditions for muscle growth and meat quality. #
The proportion of muscle fibre types and their size affect muscularity as well as functional prop... more The proportion of muscle fibre types and their size affect muscularity as well as functional properties of the musculature and meat quality. We aimed to identify QTL for microstructural muscle properties including muscle fibre size, their numbers and fibre type proportions as well as biophysical parameters of meat quality and traits related to body composition, i.e. pH, conductivity, area of M. longissimus dorsi and lean meat content. A QTL scan was conducted in a porcine experimental population that is based on Duroc and Berlin Miniature Pig.
Research article QTL for microstructural and biophysical muscle properties and body composition i... more Research article QTL for microstructural and biophysical muscle properties and body composition in pigs
The genetic variability of various local chicken populations derived from Bolivia, India, Nigeria... more The genetic variability of various local chicken populations derived from Bolivia, India, Nigeria and Tanzania was evaluated with 22 microsatellites. Between two and 11 alleles per locus were detected. All populations showed high levels of heterozygosity with the lowest value of 45% for the population named Aseel from India and the highest value of 67% for Arusha from Tanzania. A dendrogram was constructed based on CHORD distance by upgma analysis. Within this tree the populations were assorted according to their geographical origin. Bootstrapping values within the dendrogram were between 37 and 99%. The contribution of the determination of genetic variability with genetic markers to the decision on conservation and/or further use of the populations in crossbreeding programs designed to create genetic stocks with improved adaptability and productivity in tropical countries is discussed.
The identification of important genetic resources and the prevention of further loss of genetic v... more The identification of important genetic resources and the prevention of further loss of genetic variation is an important task. In developing countries selection pressure for production traits is low and traditional chicken breeds are used in rural animal production ...
Background: The proportion of muscle fibre types and their size affect muscularity as well as fun... more Background: The proportion of muscle fibre types and their size affect muscularity as well as functional properties of the musculature and meat quality. We aimed to identify QTL for microstructural muscle properties including muscle fibre size, their numbers and fibre type proportions as well as biophysical parameters of meat quality and traits related to body composition, i.e. pH, conductivity, area of M. longissimus dorsi and lean meat content. A QTL scan was conducted in a porcine experimental population that is based on Duroc and Berlin Miniature Pig. Results: Least square regression interval mapping revealed five significant and 42 suggestive QTL for traits related to muscle fibre composition under the line-cross model as well as eight significant and 40 suggestive QTL under the half-sib model. For traits related to body composition and biophysical parameters of meat quality five and twelve significant plus nine and 22 suggestive QTL were found under the line-cross and half-sib model, respectively. Regions with either significant QTL for muscle fibre traits or significant QTL for meat quality and muscularity or both were detected on SSC1, 2, 3, 4, 5, 13, 14, 15, and 16. QTL for microstructural properties explained a larger proportion of variance than did QTL for meat quality and body composition. Conclusion: Microstructural properties of pig muscle and meat quality are governed by genetic variation at many loci distributed throughout the genome. QTL analysis under both, the line-cross and half-sib model, allows detecting QTL in case of fixation or segregation of the QTL alleles among the founder populations and thus provide comprehensive insight into the genetic variation of the traits under investigation. Genomic regions affecting complex traits of muscularity and meat quality as well as microstructural properties might point to QTL that in first instance affect muscle fibre traits and by this in second instance meat quality. Disentangling complex traits in their constituent phenotypes might facilitate the identification of QTL and the elucidation of the pleiotropic nature of QTL effects.
In order to demonstrate the potential power of maternal effects on litter size and growth in pigs... more In order to demonstrate the potential power of maternal effects on litter size and growth in pigs a reciprocal cross of Berlin Miniature Pigs and Durocs was carried out. The average body weight of the Duroc dams (151 kg) was more than three times higher than the weight of the Miniature Pig dams (48 kg). A considerable maternal effect on fertility, estimated from reciprocal crossbred differences in the litter size of 3.5 piglets was found. The least Squares means of birth weight were 1.18 kg and 0.75 kg for Fl animals bom from Duroc dams and Miniature Pig dams, respectively. This maternal caused disadvantage in body weight of crossbred offspring from Miniature Pig dams is compensated in the interval from weaning to the 13th week of age. The end weight estimated by means of Gompertz and Bertalanffy growth functions showed only minor differences between the reciprocal crossed groups in favour of Fl born from Duroc dams. Our data suggest that the uterine capacity of the dam could play a...
Because of the importance of the complement system in disease resistance through direct lysis of ... more Because of the importance of the complement system in disease resistance through direct lysis of target cells and support of other mechanisms of the immune System, individual Variation in haemolytic complement activity and C3 concentration in pigs prior and after vaccination were evaluated. Possible effects on the variation of these parameters were investigated. Total complement activity was determined based on complement-mediated haemolysis of antibody-sensitised erythrocytes and C3c levels were measured by immunonephelometry in Gemian Landrace pigs and an experimental pig population. Both methods revealed remarkable individual differences. The arithmetic means of total haemolytic complement activity including all pigs were 42.3 ± 28.2 U/ml before vaccination and 59.1 ± 50.6 U/ml after three immunisations. Mean plasma concentrations of C3c were between .102 ± .035 g/1 before immunisation and .126 ± .038 g/1 on day 4 after Aujeszky vaccination. Existing p...
The genetic basis of body weight in the mouse was investigated by measuring frequency changes of ... more The genetic basis of body weight in the mouse was investigated by measuring frequency changes of microsatellite marker alleles in lines divergently selected for body weight from a base population of a cross between two inbred strains. In several regions of the genome, sharp peaks of frequency change at linked markers were detected, which suggested the presence of single genes of moderate effect, although in several other regions, significant frequency changes occurred over large portions of chromosomes. A method based on maximum likelihood was used to infer effects and map positions of quantitative trait loci (QTLs) based on genotype frequencies at one or more marker loci. Eleven QTLs with effects in the range 0.17-0.28 phenotypic standard deviations were detected; but under an additive model, these did not fully account for the observed selection response. Tests for the presence of more than one QTL in regions where there were large changes of marker allele frequency were mostly in...
In Longissimus muscle from a F 2 population of DurocÂBerlin Miniature Pigs, micro-structural fibr... more In Longissimus muscle from a F 2 population of DurocÂBerlin Miniature Pigs, micro-structural fibre traits and fatty acid composition were investigated to calculate correlation coefficients between these traits and meat quality. The animals of the F 2 population exhibited low carcass weight (55.7 AE11.2 kg), low meat percentage (35.0AE 8.4%) but a relatively high intramuscular fat content (3.52AE 1.44%) compared to pure bred animals (F 0 ). No unacceptable meat quality was observed. The variation coefficients of carcass composition, muscle fibre traits, and fat traits were high enough to allow the analysis of candidate genes which influence the growth of muscle fibres, fat cells, and meat quality. Phenotypic correlation coefficients between muscle fibre characteristics and meat quality traits were low whereas fatty acid composition and meat quality were more closely related. The correlation coefficients between muscle fibre traits and fatty acid composition ranged from 0.10 to 0.40. The relationship between a low quotient of n-6/n-3 fatty acids in muscle and greater fibre sizes, higher percentages of the oxidative fibre type and higher capillary density was noteworthy indicating good conditions for muscle growth and meat quality. #
The proportion of muscle fibre types and their size affect muscularity as well as functional prop... more The proportion of muscle fibre types and their size affect muscularity as well as functional properties of the musculature and meat quality. We aimed to identify QTL for microstructural muscle properties including muscle fibre size, their numbers and fibre type proportions as well as biophysical parameters of meat quality and traits related to body composition, i.e. pH, conductivity, area of M. longissimus dorsi and lean meat content. A QTL scan was conducted in a porcine experimental population that is based on Duroc and Berlin Miniature Pig.
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