Papers by Sepideh Alasvand
Diseases
Obesity is a condition caused by surplus adipose tissue and is a risk factor for several diet-rel... more Obesity is a condition caused by surplus adipose tissue and is a risk factor for several diet-related diseases. Obesity is a global epidemic that has also been challenging to treat effectively. However, one promoted therapy to safely treat obesity is anti-adipogenic therapeutics. Therefore, identifying potent anti-adipogenic bioactive compounds that can safely be used clinically may effectively treat obesity in humans. Mango leaf has potential medicinal properties due to its many bioactive compounds that may enhance human health. Mangiferin (MGF) is a primary constituent in mango plants, with many health-promoting qualities. Therefore, this study investigated the effect of MGF, and tea brewed with mango leaves in cultured adipocytes. The anti-adipogenic efficacy of mango leaf tea (MLT) and MGF in 3T3-L1 cells were assessed, along with cell viability, triglyceride levels, adiponectin secretion, and glucose uptake analyzed. In addition, changes in the mRNA expression of genes involved...
Nutrition Research
Mango has long been an attractive source of nutrition and pharmacological therapeutics. The mango... more Mango has long been an attractive source of nutrition and pharmacological therapeutics. The mango plant (Mangifera indica L.) contains bioactive compounds that may have antidiabetic properties. This systematic review investigated the evidence for antidiabetic properties of the different parts of the mango plant in managing type 2 diabetes mellitus in animal models and humans. The electronic databases PubMed, FSTA, Web of Science, CINAHL, MEDLINE, and Cochrane Library were systematically searched to identify articles with clear objectives and methodologies available in the English language with publication date limits up to December 2020. Twenty-eight of 1001 animal and human studies met the inclusion criteria that investigated antidiabetic properties of mango from leaf (31%), flesh (38%), seedkernel (7%), peel (14%), stem-bark (7%), and by-product (3%). Results support the glucoselowering properties of mango in both animals and human. Proposed antidiabetic mechanisms of action include inhibition of α-amylase and α-glucosidase, improved antioxidant status, improved insulin sensitivity, facilitated glucose uptake, and gene regulation of glucose transporter type 4, insulin receptor substrate 1, and phosphoinositide 3-kinase. The animal and randomized control trial findings suggest that mango may be beneficial as an antidiabetic agent. Although these studies hold promise, additional observational studies and randomized control trials are required because human studies are significantly fewer in number, use mango flesh almost exclusively, and had modest blood glucose effects. Additional research gaps include identifying the mechanisms of action for the different components of the mango plant
Current Developments in Nutrition
Objectives The objective of this study was to compare bioactive components in five teas prepared ... more Objectives The objective of this study was to compare bioactive components in five teas prepared from varieties of M. indica leaves (Tommy Atkins, Keitt, Kensington Pride, Carabao, and a commercial tea). Mango leaf tea is suggested to be rich in bioactive substances that may improve human health and provide medicinal properties. These include antioxidants, polyphenols, tannins, and Mangiferin, a major mango plant constituent. Methods Mangiferin content was measured via LC-MS/MS with column Kinetex XB-C18 (2.6 um, 100A, 150 × 3 mm) and quantified from the standard curve at 254 nm. Total polyphenols, tannins, and antioxidants were assessed by UV-VIS spectrophotometer. Results Significant differences (P < 0.05) in total polyphenols were found in all varieties except commercial tea and Tommy Atkins; the highest value was in Carabao. Higher antioxidant activity (65%) tea varieties were Carabao, commercial tea, and Kensington Pride. Keitt and Tommy Atkins had lower antioxidant activity...
Journal of Health, Medicine and Nursing, 2021
The COVID-19 pandemic which have affected every part of the world have been deemed a public healt... more The COVID-19 pandemic which have affected every part of the world have been deemed a public health concern and classified as a pandemic. The government imposing travel bans and quarantine measures have been found to be effective in preventing and reducing the spread of COVID-19. A reduction in physical activity and increase in the consumption of unhealthy foods have been observed to be some of the major implications of quarantine. Adequate physical activity and increased access to healthy foods would help in improving the psychological wellbeing of individuals thereby preventing the development of cardiovascular diseases.
Current Developments in Nutrition, 2020
Objectives This study examined the effect of common spices cardamom, ginger, cumin, curcuminoids ... more Objectives This study examined the effect of common spices cardamom, ginger, cumin, curcuminoids and cinnamon on improving glucose levels in individuals with type 2 diabetes by systematic review and meta-analysis. Methods PubMed, FSTA, Web of Science, CINAHEL, MEDLINE and Cochrane Library database of systematic review databases were searched using keywords (fasting blood glucose OR hemoglobin A1c OR HbA1c OR homeostasis) AND (Ginger or zingiber or “zingiber officinale” or “cinnamomum zeylanicum” or “cinnamomum aromaticum” or “cinnamomum cassia” or “cinnamomum verum” or curcumin or turmeric or curcuminoids or “curcuma longa” or langas or “curcuma zedoarias” or turmeric) AND (diabetes* OR “diabetes mellitus” OR “type 2” OR “blood glucose” OR insulin* OR antidiabet* OR “glucose level”) up to January 2020. Statistical calculations used SAS software version 9.2 (SAS, Cary NC, USA). P-values < 0.05 were considered significant, P-values < 0.10 were considered weakly significant. Resu...
Applied Biochemistry and Biotechnology, 2014
Microtubule-associated protein/microtubule affinity-regulating kinase 4 (MARK4) is a member of th... more Microtubule-associated protein/microtubule affinity-regulating kinase 4 (MARK4) is a member of the family Ser/Thr kinase and involved in numerous biological functions including microtubule bundle formation, nervous system development, positive regulation of programmed cell death, cell cycle control, cell polarity determination, cell shape alterations, cell division etc. For various biophysical and structural studies, we need this protein in adequate quantity. In this paper, we report a novel cloning strategy for MARK4. We have cloned MARK4 catalytic domain including 59 N-terminal extra residues with unknown function and catalytic domain alone in PQE30 vector. The recombinant MARK4 was expressed in the inclusion bodies in M15 cells. The inclusion bodies were solubilized effectively with 1.5 % N-lauroylsarcosine in alkaline buffer and subsequently purified using Ni-NTA affinity chromatography in a single step with high purity and good concentration. Purity of protein was checked on sodium dodecyl sulphate-polyacrylamide gel electrophoresis and identified by using mass spectrometry immunoblotting. Refolding of the recombinant protein was validated by ATPase assay. Our purification procedure is quick, simple and produces adequate quantity of proteins with high purity in a limited step.
Journal of Food Biochemistry, 2011
This systematic review explores ginger, cinnamon, turmeric, curcumin, and curcuminoids efficiency... more This systematic review explores ginger, cinnamon, turmeric, curcumin, and curcuminoids efficiency in improving the serum lipid profile of individuals with type 2 diabetes (T2DM). Randomized controlled trials (RCTs), reported without restriction in language, that evaluated the effectiveness of ginger, cinnamon, turmeric, curcumin and curcuminoids on lipid profile parameters (total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG)) in individuals with T2DM were investigated through January 2021. PubMed, FSTA, Web of Science, CINAHEL, MEDLINE, and Cochrane Library databases were searched using relevant keywords. The following information was extracted: first author's name, publication year, geographical location of study, study design, sample size, age and gender of participants from intervention and controls groups, intervention duration, time of the intervention, dose of spices, and type of intervent...
Current Developments in Nutrition
Objectives This systematic review and meta-analysis explored efficacy of common spices cardamom, ... more Objectives This systematic review and meta-analysis explored efficacy of common spices cardamom, ginger, cumin, curcuminoids and cinnamon on improving serum lipid levels in people with type 2 diabetes. Methods Databases PubMed, FSTA, Web of Science, CINAHEL, MEDLINE and Cochrane Library database of systematic review were searched using keywords (“Serum lipids” OR triglyceride* OR cholesterol* OR “LDL” OR “HDL” OR dyslipidemia) AND (Ginger or zingiber or “zingiber officinale” or “cinnamomum zeylanicum” or “cinnamomum aromaticum” or “cinnamomum cassia” or “cinnamomum verum” or curcumin or turmeric or curcuminoids or “curcuma longa” or langas or “curcuma zedoarias” or turmeric) AND (diabetes* OR “diabetes mellitus” OR “type 2” OR “blood glucose” OR insulin* OR antidiabet* OR "glucose level”) up to January 2020. Statistical calculations used SAS software version 9.2 (SAS, Cary NC, USA). Results Of 636 studies, 27 met the meta-analysis selection criteria, with 5698 trial participant...
Current Developments in Nutrition
Objectives The study aim was to identify bioactive compounds in mango leaf tea using a new method... more Objectives The study aim was to identify bioactive compounds in mango leaf tea using a new method - UPLC/Q-TOF-MS. Mango leaf tea is a natural product being investigated to manage type 2 diabetes due to its phytochemical properties. Methods Mango tea was extracted using 80% methanol overnight. The extract was analyzed with ultra-high-performance liquid chromatography-quadrupole time-of-flight (UPLC-Q-TOF) MS system (Waters Corp., Milford, MA) and injected into an Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 µm, Waters Corp.). The column was equilibrated with distilled water containing 0.1% formic acid (FA) and eluted by a linear gradient of acetonitrile (ACN) containing 0.1% FA at a flow rate of 0.35 mL/min for 10 min and 40 °C of column temperature. Results The eluents were analyzed by A Vion IMS Q-TOF MS (Waters Corp.) with positive and negative electrospray ionization (ESI). The scan range of TOF MS data was 50–1500 m/z, the scan time was 0.2 sec, and the capillary and sampl...
Current Developments in Nutrition
Objectives The present systematic review and meta-analysis investigates the effectiveness of S. o... more Objectives The present systematic review and meta-analysis investigates the effectiveness of S. officinalis leaves in management of dyslipidemia of chronic disease. Dyslipidemia is one of the main indicators of cardiovascular disease (CVD). It is typically characterized by elevated levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL) and triglyceride (TG) along with reduction of high-density lipoprotein cholesterol (HDL) in blood. The handling of dyslipidemia is a crucial factor in the general control of the cardiovascular risk in individuals suffering from diseases such as diabetes, CVD or hypertension. Plant foods have played an important role in chronic disease management. S. officinalis leaves (sage) have been identified as improving glucose homeostasis and serum lipids in animal and human models. Methods PubMed, FSTA, Web of Science, CINAHEL and MEDLINE databases were searched using keywords (“Serum lipids” OR triglyceride* OR cholesterol* OR “LDL” OR “HD...
Current Developments in Nutrition
Objectives A meta-analysis systematic review that investigates the impact of the herbal tea (non-... more Objectives A meta-analysis systematic review that investigates the impact of the herbal tea (non-camellia sinensis) on glucose homeostasis and serum lipids in individuals with type 2 diabetes was conducted in response to a growing interest in herbal remedies as a potential alternative source of management of type 2 diabetes. Diabetes mellitus (DM) is a metabolic disorder that has contributed to the US burden of morbidity, disability and mortality with a fast-increasing global trend through the last decades. Methods PubMed, FSTA, Web of Science, CINAHEL and MEDLINE databases were searched using keywords diabetes* OR “diabetes mellitus” OR “type 2” OR “blood glucose” OR insulin* OR antidiabet* OR “glucose level”) AND (“Serum lipids” OR triglyceride* OR cholesterol* OR “LDL” OR “HDL” OR dyslipidemia) AND (“non-camellia sinensis” OR tea) up to January 2019. Review articles, animal studies, non-trial, non-herbal tea (green, black and white tea), and articles which did not evaluate glucos...
Asian-Australasian journal of animal sciences, 2013
This study was performed to compare physicochemical and sensory properties of cholesterol-removed... more This study was performed to compare physicochemical and sensory properties of cholesterol-removed Gouda cheese (CRGC) and Gouda cheese made in the laboratory during ripening. Composition, short-chain free fatty acids (SCFFA), texture, color, and sensory properties were measured. In chemical composition analyses, moistures were significantly different between control cheeses (42.86%) and sample cheese (48.32%) (p<0.05). But fat and protein in the control and the sample were 32.77, 22.45 and 31.35, 20.39%, respectively, and were not significantly different (p>0.05). The amount of cholesterol in control was 82.52 mg/100 g and the percentage of cholesterol removal was 90.7%. SCFFA increased gradually during ripening and its level of CRGC increased and significantly different from that of control (p<0.05). The texture, hardness, gumminess, and chewiness were significantly increased, but cohesiveness and springiness were not increased in both cheeses during ripening periods (p>...
Objectives: This study examined the effect of common spices cardamom, ginger, cumin, curcuminoids... more Objectives: This study examined the effect of common spices cardamom, ginger, cumin, curcuminoids and cinnamon on improving glucose levels in individuals with type 2 diabetes by systematic review and meta-analysis. Methods: PubMed, FSTA, Web of Science, CINAHEL, MEDLINE and Cochrane Library database of systematic review databases were searched using keywords (fasting blood glucose OR hemoglobin A1c OR HbA1c OR homeostasis) AND (Ginger or zingiber or "zingiber of-zedoarias" or turmeric) AND (diabetes * OR "diabetes mellitus" OR "type 2" OR "blood glucose" OR insulin * OR antidiabet * OR "glucose level") up to January 2020. Statistical calculations used SAS software version 9.2 (SAS, Cary NC, USA). P-values < 0.05 were considered significant, P-values < 0.10 were considered weakly significant. Results: Twenty-three studies with 2237 trial participants were selected out of a 512 study pool. Spices decreased FBG and HbA1c %. The estimated reduction in intervention change vs. control change is as follows: A1c was −0.264 mg/dl, 95% CI (−0.5503, −0.02), P value 0.069; FBG was −9.9370 mg/dl, 95% CI (−20.79, 0.91), P value 0.07. A significant heterogeneity was observed overall among the all studies, indicating that not all studies had similar levels of decrease. Conclusions: A correlation between consumption of certain common spices and significant reduction in glycemic indices among diabetes patients was proved.
Objectives: This systematic review and meta-analysis explored efficacy of common spices cardamom,... more Objectives: This systematic review and meta-analysis explored efficacy of common spices cardamom, ginger, cumin, curcuminoids and cinnamon on improving serum lipid levels in people with type 2 diabetes.
Objectives: The study aim was to identify bioactive compounds in mango leaf tea using a new metho... more Objectives: The study aim was to identify bioactive compounds in mango leaf tea using a new method-UPLC/Q-TOF-MS. Mango leaf tea is a natural product being investigated to manage type 2 diabetes due to its phytochemical properties. Methods: Mango tea was extracted using 80% methanol overnight. The extract was analyzed with ultra-high-performance liquid chromatography-quadrupole time-of-flight (UPLC-Q-TOF) MS system (Waters Corp., Milford, MA) and injected into an Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 µm, Waters Corp.). The column was equilibrated with distilled water containing 0.1% formic acid (FA) and eluted by a linear gradient of acetonitrile (ACN) containing 0.1% FA at a flow rate of 0.35 mL/min for 10 min and 40°C of column temperature. Results: The eluents were analyzed by A Vion IMS Q-TOF MS (Waters Corp.) with positive and negative electrospray ionization (ESI). The scan range of TOF MS data was 50-1500 m/z, the scan time was 0.2 sec, and the capillary and sampling cone voltages were 3 kV and 40 V, respectively. The desolvation flow rate and temperature were 800 L/h and 400°C, respectively, and the source temperature was 100°C. Leucine-enkephalin ([M + H] = 556.2771 Da; [M-H] = 554.2615 Da), used as a lack mass, was infused at a flow rate of 0.35 μL/min and a frequency of 10 s to ensure mass measurement accuracy of the metabolites analyzed by the instrument. Mass spectral data were collected from m/z 50 to 1500 with a scan time of 0.2 s. Collision energy was ramped from 10 to 40 eV for the collection of MS/MS data. The metabolites Mangiferin, quercetin/isoquercetin and iriflophenone 3-beta-glucoside were identified using ChemSpider in negative mode while only mangiferin and quercetin were defined in positive mode with different intensites: Mangiferin (421.07), quercetin/isoquercetin (303.05) and irrflophenone 3-beta-glucoside (407.097) that confirmed their molar mass. Conclusions: The UPLC/Q-TOF-MS technique is a practical and novel method for identifying the fragmentation pattern of metabolites in mango leaf tea by measuring accurate mass with excellent resolution and sensitivity.
Journal of Health, Medicine and Nursing, 2021
The COVID-19 pandemic which have affected every part of the world have been deemed a public healt... more The COVID-19 pandemic which have affected every part of the world have been deemed a public health concern and classified as a pandemic. The government imposing travel bans and quarantine measures have been found to be effective in preventing and reducing the spread of COVID-19. A reduction in physical activity and increase in the consumption of unhealthy foods have been observed to be some of the major implications of quarantine. Adequate physical activity and increased access to healthy foods would help in improving the psychological wellbeing of individuals thereby preventing the development of cardiovascular diseases.
Chymosin is an aspartic proteinase found in the stomach of neonatal mammals and is used as milk c... more Chymosin is an aspartic proteinase found in the stomach of neonatal mammals and is used as milk coagulant in the cheese industry. In this study, preprochymosin cDNA from the abomasums of Iranian natives' kid goats was cloned and characterized. This cDNA has an open reading frame of 1143 bp and is predicted to code for a preproenzyme of 381 amino acids with an N-terminal 16 amino acid signal peptide that is followed by a 42 amino acid proenzyme. The deduced amino acid sequence revealed 98.7% and 94% identity with corresponding lamb and cattle sequences, respectively. The cDNA encoding for prochymosin was then subcloned into pET-28a and expressed with and without N-terminal His-tag in E. coli BL 21 (DE 3). Inclusion bodies were isolated and solubilized in 8 M urea at pH 10.7. Solubilized prochymosion molecules were successfully refolded and active recombinant goat chymosin was recovered after subsequent activation. Milk clotting activity (416 U/mg and 192 U/mg) was observed in (His +)-prochymosin and (His-)-prochymosin enzymes, respectively, after activation.
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Papers by Sepideh Alasvand