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    Protein Tertiaty Structure Prediction

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    Roshan Mammen Chakkungal
    The document discusses different methods for predicting protein tertiary structure, including homology modeling, threading, and ab initio prediction. Homology modeling and threading use known protein structural information in databases to build models, while ab initio prediction attempts to predict structures based solely on sequence information without templates by searching for energetically favorable conformations. Current ab initio methods are limited by incomplete understanding of protein folding and lack of accurate energy functions.

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    Protein Tertiaty Structure Prediction

    Uploaded by

    Roshan Mammen Chakkungal
    141 views12 pages
    The document discusses different methods for predicting protein tertiary structure, including homology modeling, threading, and ab initio prediction. Homology modeling and threading use known protein structural information in databases to build models, while ab initio prediction attempts to predict structures based solely on sequence information without templates by searching for energetically favorable conformations. Current ab initio methods are limited by incomplete understanding of protein folding and lack of accurate energy functions.

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    Threading

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    The document discusses different methods for predicting protein tertiary structure, including homology modeling, threading, and ab initio prediction. Homology modeling and threading use known protein structural information in databases to build models, while ab initio prediction attempts to predict structures based solely on sequence information without templates by searching for energetically favorable conformations. Current ab initio methods are limited by incomplete understanding of protein folding and lack of accurate energy functions.

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    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    141 views12 pages

    Protein Tertiaty Structure Prediction

    Uploaded by

    Roshan Mammen Chakkungal
    The document discusses different methods for predicting protein tertiary structure, including homology modeling, threading, and ab initio prediction. Homology modeling and threading use known protein structural information in databases to build models, while ab initio prediction attempts to predict structures based solely on sequence information without templates by searching for energetically favorable conformations. Current ab initio methods are limited by incomplete understanding of protein folding and lack of accurate energy functions.

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    PROTEIN TERTIATY STRUCTURE PREDICTION

    Important scientific achievements of the twentieth century was the discovery of the DNA double helical structure by Watson and Crick in 1953. The work was the result of a three-dimensional modeling conducted partly based on data obtained from x-ray diffraction of DNA and partly based on chemical bonding information established in stereochemistry. Watson and Crick conducted one of the first-known ab initio modeling of a biological macromolecule, which has subsequently been proven to be essentially correct. Their work provided great insight into the mechanism of genetic inheritance. The much slower rate of structure determination by x-ray crystallography or NMR spectroscopy compared to gene sequence generation from genomic studies. Consequently, the gap between protein sequence information and protein structural information is increasing rapidly. Protein structure prediction aims to reduce this sequencestructure gap.

    In contrast to sequencing techniques, experimental methods to determine protein structures are time consuming and limited in their approach. Currently, it takes 1 to 3 years to solve a protein structure. Certain proteins, especially membrane proteins, are extremely difficult to solve by x-ray or NMR techniques. There are many important proteins for which the sequence information is available, but their three-dimensional structures remain unknown. The full understanding of the biological roles of these proteins requires knowledge of their structures. Hence, the lack of such information hinders many aspects of the analysis, ranging from protein function and ligand binding to mechanisms of enzyme catalysis. Therefore, it is often necessary to obtain approximate protein structures through computer modeling. Having a computer-generated three-dimensional model of a protein of interest has many ramifications, assuming it is reasonably correct. It may be of use for the rational design of biochemical experiments, such as sitedirected mutagenesis, protein stability, or functional analysis.

    METHODS
    There are three computational approaches to protein three-dimensional structural modeling and prediction. They are homology modeling, threading, and ab initio prediction. The first two are knowledge-based methods; they predict protein structure based on knowledge of existing protein structural information in databases. Homology modeling builds an atomic model based on an experimentally determined structure that is closely related at the sequence level. Threading identifies proteins that are structurally similar, with or without detectable sequence similarities. The ab initio approach is simulation based and predicts structures based on physicochemical principles governing protein folding without the use of structural templates.

    THREADING AND FOLD RECOGNITION


    There are only small number of protein folds available (<1,000), compared to millions of protein sequences. This means that protein structures tend to be more conserved than protein sequences. Consequently ,many proteins can share a similar fold even in the absence of sequence similarities. This allowed the development of computational methods to predict protein structures beyond sequence similarities. To determine whether a protein sequence adopts a known threedimensional structure fold relies on threading and fold recognition methods.

    Pairwise Energy Method


    In the pairwise energy based method, aprotein sequence is searched for in a structural fold database to find the best matching structural fold using energy-based criteria. The detailed procedure involves aligning the query sequence with each structural fold in a fold library. The alignment is performed essentially at the sequence profile level using dynamic programming or heuristic approaches. Local alignment is often adjusted to get lower energy and thus better fitting. The next step is to build a crude model for the target sequence by replacing aligned residues in the template structure with the corresponding residues in the query. The third step is to calculate the energy terms of the raw model, which include pairwise residue interaction energy, solvation energy, and hydrophobic energy. Finally, the models are ranked based on the energy terms to find the lowest energy fold that corresponds to the structurally most compatible fold

    AB INITIO PROTEIN STRUCTURAL PREDICTION


    Both homology and fold recognition approaches rely on the availability of template structures in the database to achieve predictions. If no correct structures exist in the database, the methods fail. However, proteins in nature fold on their own without checking what the structures of their homologs are in databases. Obviously, there is some information in the sequences that provides instruction for the proteins to find their native structures. Early biophysical studies have shown that most proteins fold spontaneously into a stable structure that has near minimum energy. This structural state is called the native state. This folding process appears to be non random;however, its mechanism is poorly understood.

    The limited knowledge of protein folding forms the basis of ab initio prediction. As the name suggests, the ab initio prediction method attempts to produce all-atom protein models based on sequence information alone without the aid of known protein structures. The perceived advantage of this method is that predictions are not restricted by known folds and that novel protein folds can be identified. However, because the physicochemical laws governing protein folding are not yet well understood, the energy functions used in the ab initio prediction are at present rather inaccurate. The folding problem remains one of the greatest challenges in bioinformatics today. Current ab initio algorithms are not yet able to accurately simulate the protein folding process. They work by using some type of heuristics. Because the native state of a protein structure is near energy minimum, the prediction programs are thus designed using the energy minimization principle. These algorithms search for every possible conformation to find the one with the lowest global energy

    Searching for a fold with the absolute minimum energy may not be valid in reality. This contributes to one of the fundamental flaws of this approach. In addition, searching for all possible structural conformations is not yet computationally feasible. It has been estimated that, by using one of the worlds fastest supercomputers (one trillion operations per second), it takes 10 20 years to sample all possible conformations of a 40-residue protein. Therefore, some type of heuristics must be used to reduce the conformational space to be searched. Some recent ab initio methods combine fragment search and threading to yield a model of an unknown protein

    Rosetta (www.bioinfo.rpi.edu/bystrc/hmmstr/server.php) is a web server that predicts protein three-dimensional conformations using the ab initio method. This in fact relies on a mini-threading method. The method first breaks down the query sequence into many very short segments (three to nine residues) and predicts the secondary structure of the small segments using a hiddenMarkov modelbased program, HMMSTR, The segments with assigned secondary structures are subsequently assembled into a three-dimensional configuration. Through random combinations of the fragments, a large number of models are built and their overall energy potentials calculated. The conformation with the lowest global free energy is chosen as the best model. It needs to be emphasized that up to now, Ab initio prediction algorithms are far from mature. Their prediction accuracies are too low to be considered practically useful. Ab initio prediction of protein structures remains a fanciful goal for the future.

    However, with the current pace of high-throughput structural determination by the structural proteomics initiative, which aims to solve all protein folds within a decade, the time may soon come when there is little need to use the Ab initio modeling approach because homology modeling and threading can provide much higher quality predictions for all possible protein folds. Regardless of the progress made in structural proteomics, exploration of protein structures using the Ab initio prediction approach may still yield insight into the protein-folding process.

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