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    Rhodobacter Maliha Rashid

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    Maliha Rashid
    Rhodobacter; a microbial cell factory A presentation on the article: "The transition of Rhodobacter sphaeroides into a microbial cell factory" by Enrico Orsi et al. 2020. In these slides, focus is on: 1. Understanding the microbial cell factories with Rhodobacter sphaeroides as an example 2. Understand the DBTL concept 3. Analyze how DBTL pipeline is incorporated in Rhodobacter sphaeroides 4. Why the use of such a streamlined method for strain engineering is proposed 5. Industrial applications of this platform

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    Rhodobacter Maliha Rashid

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    Maliha Rashid
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    Rhodobacter; a microbial cell factory A presentation on the article: "The transition of Rhodobacter sphaeroides into a microbial cell factory" by Enrico Orsi et al. 2020. In these slides, focus is on: 1. Understanding the microbial cell factories with Rhodobacter sphaeroides as an example 2. Understand the DBTL concept 3. Analyze how DBTL pipeline is incorporated in Rhodobacter sphaeroides 4. Why the use of such a streamlined method for strain engineering is proposed 5. Industrial applications of this platform

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    Rhodobacter; a microbial cell factory A presentation on the article: "The transition of Rhodobacter sphaeroides into a microbial cell factory" by Enrico Orsi et al. 2020. In these slides, focus is on: 1. Understanding the microbial cell factories with Rhodobacter sphaeroides as an example 2. Understand the DBTL concept 3. Analyze how DBTL pipeline is incorporated in Rhodobacter sphaeroides 4. Why the use of such a streamlined method for strain engineering is proposed 5. Industrial applications of this platform

    Copyright:

    © All Rights Reserved
    57 views16 pages

    Rhodobacter Maliha Rashid

    Uploaded by

    Maliha Rashid
    Rhodobacter; a microbial cell factory A presentation on the article: "The transition of Rhodobacter sphaeroides into a microbial cell factory" by Enrico Orsi et al. 2020. In these slides, focus is on: 1. Understanding the microbial cell factories with Rhodobacter sphaeroides as an example 2. Understand the DBTL concept 3. Analyze how DBTL pipeline is incorporated in Rhodobacter sphaeroides 4. Why the use of such a streamlined method for strain engineering is proposed 5. Industrial applications of this platform

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    Rhodobacter sphaeroides

    as Microbial Cell Factory


    Design, Built, Test, Learn
    Concept
    Maliha Rashid

    1
    About the Article

    Access to Document
    10.1002/bit.27593

    2
    Original language English

    Impact Factor 4.395

    Pages (from-to) 531-541

    Journal Biotechnology and Bioengineering

    Volume 118

    Issue number 2

    Early online date 10 Oct 2020

    Publication status Published - Feb 2021

    3
    Article Overview

    Rhodobacter sphaeroides is a class of bacteria that have the potential to synthesize industrially important products as
    microbial cell factories

    Learning objectives

    • Understanding the microbial cell factories with Rhodobacter sphaeroides as an example


    • Understand the DBTL concept
    • Analyze how DBTL pipeline is incorporated in Rhodobacter sphaeroides
    • Why the use of such a streamlined method for strain engineering is proposed
    • Industrial applications of this platform

    Keywords

    DBTL cycles, industrial biotechnology, metabolic engineering, microbial cell factory,

    4
    What makes R.sphaeroides an ideal choice as a microbial cell
    factories?

    There are several reasons justifying the interest in R. sphaeroides as chassis for biotechnological productions:

    1. Used as a model organism for studying anoxygenic photosynthesis, but also chemotaxis and quorum sensing

    2. It displays high metabolic versatility - thrive by aerobic or anaerobic respiration and anoxygenic photosynthesis

    3. R. sphaeroides is a natural producer of relevant bio‐ based compounds such as

    • Isoprenoids

    • poly‐β‐hydroxybutyrate (PHB)

    • Hydrogen

    03/02/2024 5
    Overview of the bioeconomy pyramid. This is divided into different sectors based in the market volume
    and price per kilo of the compounds produced. In the green boxes, compounds produced by
    Rhodobacter sphaeroides are shown. They are allocated to the respective target market sector
    03/02/2024 6
    Design, Built, Test, Learn (DBTL) Concept

    7
    Different Research Fields Contributed To The DBTL Method
    In R. Sphaeroides

    Establishment of a DBTL pipeline in a


    chassis requires contribution from
    different research fields, including
    • ‐omics techniques
    • genome engineering
    • phenotypic screening methods

    8
    Pathways involved in the carbon metabolism of Rhodobacter sphaeroides

    Entner Doudoroff
    Pathway

    Embden Meyerhoff
    Pathway

    Mevolonate Pathway
    (MVA)

    2‐C‐methyl‐D‐erythritol 4‐
    phosphate (MEP)
    03/02/2024 9
    Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides

    Fig: overview of the MEP operon including the target gene dxr. All the other genes
    included are involved in growth‐related functions

    Fig: overview of the MVA operon from the α‐proteobacterium Paracoccus


    zeaxanthinifaciens

    Fig: Overview of the strategy


    for isoprenoid pathway
    replacement in Rhodobacter
    sphaeroides.

    Microbial Biotechnology, Volume: 13, Issue: 4, Pages: 1082-1093, First published: 24 March 2020, DOI: (10.1111/1751-7915.13562)
    Figure: Lumped network of the carbon metabolism of Rhodobacter
    sphaeroides, including pathways for substrate uptake and product
    formation.

    Substrates are highlighted in different colors, each


    describing a different growth mode
    • light blue: chemoheterotrophic
    • light orange: photoheterotrophic
    • light yellow: photo‐ or chemolitho‐autotrophic

    The three carbon products described in this review


    are highlighted
    1. 5‐aminolevulinic acid (5‐ALA, yellow)
    2. isoprenoids (red)
    3. poly‐β‐hydroxybutyrate (PHB, green)

    Some pathways with parallel flux are highlighted,


    for example, glycolysis:
    • Emden–Meyerhof–Parnas (red)
    • Entner–Doudoroff (gray)
    • isoprenoid synthesis: 2‐C‐methyl‐D‐erythritol 4‐
    phosphate (MEP) pathway (blue)
    • mevalonate pathway (orange)

    11
    An Outlook on the Industrial Applications Of R. Sphaeroides:
    Current Situation and Future Perspectives

    This microorganism is considered nonpathogenic and generally regarded as safe (GRAS).

    R. sphaeroides is being employed in few companies worldwide:

    Company Name Website link Area of bacterial use

    produces sesquiterpenes as aroma


    Dutch flavors and fragrances company Isobionics BV www.isobionics.com
    compunds

    Chinese company CN Lab Nutrition www.cnlabnutrition.com Nutraceuticals, coenzyme Q10 with a rate
    of up to 30 tons per month.
    Chinese company Hebei Shixiang Biological Technology Co.,
    www.hbshixiang.en.china.cn feed industry for livestock
    Ltd.

    Indian company Prions Biotech www.prionsbiotech.com Fish feed solutions

    12
    Future Perspectives
    the use of cheap and renewable
    feedstocks could improve both
    This microorganism could be exploited for evaluating
    alternative feedstocks for the bio‐based economy commercial and experimental processes
    because of where R. sphaeroides is involved

    • its versatile metabolism


    Technologies used in DBTL pipeline could be
    • its wide substrate acceptance range
    used in R. sphaeroides to optimize this
    • recent advancements in its DBTL method platform for assimilation of C1 substrates,
    while coupling it to synthesis to a range of
    bio‐based compounds

    13
    Take home Message

    • R. sphaeroides has the potential to produce industrially important bioproducts

    • Moreover, it is reasoned that recent improvements in the DBTL pipeline can

    be employed for further optimizing this cell factory for industrial applications

    of other organisms

    03/02/2024 14
    References

    1. Microbial Biotechnology, Volume: 13, Issue: 4, Pages: 1082-1093, First published: 24 March 2020, DOI:
    (10.1111/1751-7915.13562)

    2. Enrico Orsi, Jules Beekwilder, Gerrit Eggink, Servé W. M. Kengen, Ruud A. Weusthuis. The transition
    of Rhodobacter sphaeroides into a microbial cell factory (2020). https://doi.org/10.1002/bit.27593

    03/02/2024 15
    25
    min

    THANK you

    Have a nice day

    03/02/2024 16

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