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    Microscope Parts and Function

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    Kimberly Villarosa
    The document describes the main parts of a light microscope and their functions. It includes labels for the tube, nose piece, objectives of different magnifications, stage clips, diaphragm, light source, eye piece, neck, stage, coarse and fine adjustments, base. It explains that total magnification is calculated by multiplying the objective and eye piece magnifications. It provides a brief history of microscope development and types of microscopes including compound light, electron, transmission electron and scanning electron microscopes. It outlines general procedures for microscope use, focusing specimens, and emphasizes only using the fine adjustment when focusing with the high power objective.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    Microscope Parts and Function

    Uploaded by

    Kimberly Villarosa
    31 views28 pages
    The document describes the main parts of a light microscope and their functions. It includes labels for the tube, nose piece, objectives of different magnifications, stage clips, diaphragm, light source, eye piece, neck, stage, coarse and fine adjustments, base. It explains that total magnification is calculated by multiplying the objective and eye piece magnifications. It provides a brief history of microscope development and types of microscopes including compound light, electron, transmission electron and scanning electron microscopes. It outlines general procedures for microscope use, focusing specimens, and emphasizes only using the fine adjustment when focusing with the high power objective.

    Original Description:

    parts of microscope

    Original Title

    MICROSCOPE PARTS AND FUNCTION

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    The document describes the main parts of a light microscope and their functions. It includes labels for the tube, nose piece, objectives of different magnifications, stage clips, diaphragm, light source, eye piece, neck, stage, coarse and fine adjustments, base. It explains that total magnification is calculated by multiplying the objective and eye piece magnifications. It provides a brief history of microscope development and types of microscopes including compound light, electron, transmission electron and scanning electron microscopes. It outlines general procedures for microscope use, focusing specimens, and emphasizes only using the fine adjustment when focusing with the high power objective.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    31 views28 pages

    Microscope Parts and Function

    Uploaded by

    Kimberly Villarosa
    The document describes the main parts of a light microscope and their functions. It includes labels for the tube, nose piece, objectives of different magnifications, stage clips, diaphragm, light source, eye piece, neck, stage, coarse and fine adjustments, base. It explains that total magnification is calculated by multiplying the objective and eye piece magnifications. It provides a brief history of microscope development and types of microscopes including compound light, electron, transmission electron and scanning electron microscopes. It outlines general procedures for microscope use, focusing specimens, and emphasizes only using the fine adjustment when focusing with the high power objective.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    You are on page 1of 28

    Parts of the Microscope

    and Their Function


    #1 Tube –
    Reflects light
    up to the
    viewers eye
    #2 Nose piece –
    Allows for quick
    change of
    objectives
    #3 Low Power
    Objective – The first
    lens you use when
    doing proper
    microscope work.
    Usually 4 X
    #4 Medium Power
    Objective – The
    second lens you use
    when doing proper
    microscope work.
    Usually 10 X
    #5 High Power
    Objective – The
    highest
    magnification used.
    Usually 43 X. NEVER
    use the course
    adjustment when
    using this lens.
    #6 Stage
    Clips – Use
    to keep the
    slide in
    place.
    #7 Diaphragm –
    Use to vary the
    amount of light
    passing through
    the slide. Usually
    it is better if the
    amount of light is
    low.
    #8 Light Source –
    Sends light up
    through the
    diaphragm and
    through the slide
    for viewing
    #9 Eye Piece—
    The part you
    look at with
    your eye.
    Usually 10 X
    magnification.
    #10 Neck –
    Used to
    safely
    transport
    microscope
    #11 Stage –
    Slides are
    placed on
    this
    #12 Coarse
    Adjustment – Used
    to make large
    changes in focus.
    NOTE: Never use
    this when viewing
    on high power
    #13 Fine
    Adjustment –
    Used to small
    adjustments of
    focus
    #14 Base –
    Used to safely
    transport the
    microscope
    Magnification
    Your microscope has 3 magnifications: Scanning, Low and High. Each objective
    will have written the magnification. In addition to this, the ocular lens (eyepiece)
    has a magnification. The total magnification is the ocular x objective
    How a Light Microscope Works
    1.Use lenses to make small objects appear larger
    2.Compound light microscope: Two lenses
    separated by a tube
    3.Lenses magnify an object by bending the light
    that passes through the lens
    4.Magnification: ability to make things appear
    larger than they are
    5.Resolution: fineness of detail that can be seen in
    an image

    Go to
    Section:
    HISTORY OF MICROSCOPE
    •1590 –first compound microscope
    • 1655 – Robert Hooke used a compound microscope
    to observe pores in cork.

    • He called them “cells”


    •Antoine van
    Leeuwenhoek
    •1 to see single-
    st

    celled organisms
    in pond water
    Types of Microscopes
    • 1. Compound Light
    Microscope
    • 1st type of microscope,
    most widely used
    • light passes through 2
    lenses
    • Can magnify up to
    2000x
    Types of Microscopes
    • 2. Electron Microscope
    • Used to observe VERY small
    objects: viruses, DNA, parts
    of cells
    • Uses beams of electrons
    rather than light
    • Much more powerful
    Types of Microscopes
    • 3.Transmission
    Electron Microscope
    (TEM)
    • Can magnify up to
    250,000x
    Types of Microscopes
    •4.Scanning Electron
    Microscope (SEM)
    • Can magnify up to
    100,000x
    General Procedures
    1.Make sure all backpacks and materials are out of the aisles and
    off the tops of desks. 
    2.Plug your microscope in to the outlet.
    3.Store with cord wrapped around microscope and the scanning
    objective clicked into place.
    4.Carry by the base and arm with both hands.
    Focusing Specimens
    1. Always start with the scanning objective. 
    Use the Coarse Knob to focus and then the fine adjustment knob until clear,
    image may be small at this magnification.
    2. Once you've focused on Scanning, switch to Low Power.
    Use the Coarse Knob to refocus, then fine adjustment until clear.
    Now switch to High Power.. At this point, ONLY use the Fine
    Adjustment Knob to focus specimens.
    • Your slide MUST be focused on low power before
    attempting this step
    • Click the nosepiece to the longest objective
    • Do NOT use the Coarse Focusing Knob, this could
    crack the slide or the lens
    • Use the Fine Focus Knob to bring the slide
    • Emphasize not using the coarse
    objective during this process, as it
    will crack the slides.

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