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    Fungal Culture

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    hersey miayo
    This document summarizes various culture media and methods used for cultivating fungi. It describes different types of media for primary recovery, differential testing, and identification of fungi. It also outlines incubation conditions and procedures for macroscopic and microscopic examination of fungal cultures, including assessing colony characteristics, pigmentation, texture, and using slide cultures.

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    Fungal Culture

    Uploaded by

    hersey miayo
    53 views7 pages
    This document summarizes various culture media and methods used for cultivating fungi. It describes different types of media for primary recovery, differential testing, and identification of fungi. It also outlines incubation conditions and procedures for macroscopic and microscopic examination of fungal cultures, including assessing colony characteristics, pigmentation, texture, and using slide cultures.

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    This document summarizes various culture media and methods used for cultivating fungi. It describes different types of media for primary recovery, differential testing, and identification of fungi. It also outlines incubation conditions and procedures for macroscopic and microscopic examination of fungal cultures, including assessing colony characteristics, pigmentation, texture, and using slide cultures.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    53 views7 pages

    Fungal Culture

    Uploaded by

    hersey miayo
    This document summarizes various culture media and methods used for cultivating fungi. It describes different types of media for primary recovery, differential testing, and identification of fungi. It also outlines incubation conditions and procedures for macroscopic and microscopic examination of fungal cultures, including assessing colony characteristics, pigmentation, texture, and using slide cultures.

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    © All Rights Reserved
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    CULTIVATION OF

    FUNGI
    • Brain-heart infusion agar: saprobic and pathogenic fungi
    • Brain-heart infusion agar with antibiotics: pathogenic fungi, yeast
    • Brain-heart infusion biphasic blood culture bottles: Recovery of
    fungi from blood
    • Dermatophyte test medium: dermatophytes, recommended as
    screening medium only
    • Inhibitory mold agar: pathogenic fungi exclusive of dermatophytes
    • Potato flake agar : pathogenic fungi
    • Mycosel: dermatophytes
    • SABHI agar: saprobic and pathogenic fungi
    • Yeast-extract phosphate agar: pathogenic fungi, Blastomyces
    dermatitidis and Histoplasma capsulatum
    • Chromogenic agar: yeast and filamentous fungi

    Primary Recovery Media


    • Ascospore agar: Detection of ascospores in Saccharomyces spp.
    • Cornmeal agar with Tween 80 and trypan blue: Identification of C.
    albicans by chlamydospore production
    • Cottonseed conversion agar : Conversion of dimorphic fungus B.
    dermatitidis from mold to yeast form
    • Czapek’s agar: Differential identification of Aspergillus spp.
    • Niger seed agar: Identification of C. neoformans
    • Nitrate reduction medium: Detection of nitrate reduction in confirmation
    of Cryptococcus spp.
    • Christensen’s Urea agar: Cryptococcus, Trichosporon, Rhodotorula
    • Potato Dextrose agar: pigment production of Trichophyton rubrum,
    sporulation of dermatophyte
    • Yeast fermentation broth
    • Yeast nitrogen base agar
    Differential Test Media
    • Sabouraud dextrose agar (SDA), SDA with antimicrobial agents,
    potato dextrose agar (PDA) or the slightly modified potato flakes
    agar, and BHI agar enriched with blood and antimicrobial agents.

    • Gentamicin or chloramphenicol and cycloheximide (mycosel agar)


    • Gentamicin and chloramphenicol inhibit bacterial growth

    • cycloheximide inhibits bacteria and many of the environmental


    fungi typically considered contaminants

    • Tubed media have the advantage of being safer to handle and less
    susceptible to drying.

    Culture Media
    • room temperature or at 30° C
    • dimorphic fungus: 37° C
    • 4 to 6 weeks
    • should be examined twice weekly for growth.

    Incubation
    • Gross morphologic traits: color, texture, growth rate

    • Pigment: reverse side of the colony or in the aerial mycelium

    • Colony topography: various elevations of the colony on the agar plate.


    • Verrucose: furrowed or convoluted
    • Umbonate: slightly raised center
    • Rugose: furrows radiate out from the center

    • Texture:
    • Cottony: loose high aerial mycelium
    • Velvety: low aerial mycelium
    • Glabrous: smooth surface with no aerial mycelium
    • Granular: dense, powdery resembling sugar granules
    • Wooly: high aerial mycelium
    Macroscopic Examination of Cultures
    • useful for demonstrating the natural morphology of
    fungal structures and for encouraging conidiation in some
    poorly fruiting fungi

    • advantage to this type of slide is that it can be preserved


    indefinitely

    Slide Culture

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