Microbial Control For Plant Disease in Thailand: Watchalee Sopin
Microbial Control For Plant Disease in Thailand: Watchalee Sopin
Microbial Control For Plant Disease in Thailand: Watchalee Sopin
PLANT DISEASE
IN THAILAND
Watchalee Sopin
TRICHODERMA ON PDA
TRICHODERMA HARZIANUM
fungi (coiling/penetration/lysing)
* Strong competition for substrates
* Produce antibiotics (pyrone/oxazole)
* Induce resistance against Pythium root rot
of cucumber
EFFICACY OF TRICHODERMA
FOR CONTROLLING PLANT
DISEASES
PHYTOPHTHORA ROOT ROT OF DURIAN
: P hytophthora palmivora
DAMPING-OFF OF COTTON
: Rhizoctonia solani
DAMPING-OFF - Pythium aphanidermatum
tomato
Vegetable soybean
STEM ROT OF SOYBEAN
- Pythium sp.
STEM ROT OF TOMATO - Sclerotium rolfsii
STEM ROT AND WILT OF YARD LONG BEAN - Sclerotium rolfsii
STEM ROT OF CHILLI
- Sclerotium rolfsii
ANTRACNOSE OF CHILLI - Colletotrichum capsici
STEM ROT OF LETTUCE
Pythium aphanidermatum
ROOT ROT OF LETTUCE
Pythium spp.
BLACK LEAF MOLD OF TOMATO
Pseudocercospora fuligena
ANTRACNOSE ON LEAF OF MANGO
Colletotrichum gloeosporioides
SHOOT ROT OF ORCHID
Sclerotium rolfsii
SHEATH BLIGHT OF RICE
Rhizoctonia solani
DIRTY PANICLE OF RICE
BROWN SPOT OF RICE
Helminthosporium oryzae
DRY POWDER
TRICHODERMA
FORMULATIONS
LIQUID
Compost (100 kg.)
Rice bran
Trichoderma fresh culture
Rice bran
Trichoderma fresh culture
+ Rice bran Compost
Trichoderma + Rice bran + Compost
50-100 g / m2
Broadcasting during soil preparation
Planting seeds
Mulching with straw
Watering
Seedings / Growing period
Broadcasting under plant canopy 50-100 g / m2
10-20 g / plant
10-20 g / plant
10-20 g / hole
500-1000 g / hole
Planting medium Trichoderma fresh culture
+ Rice bran + Compost
4 parts 1 part
MIXING
Trichoderma
coated seeds
1-2 L
WATER
FILTER
1 Kg Trichoderma
PRESS/ SHAKING / SQUEEZE (gently)
WASHING
FILTERING
(through screen)
Trichoderma suspension
1-2 L – Trichoderma
suspension + 200 L - water
SPRAY GERMINATED SEEDLINGS
SPRAY WHOLE PLANT AND SOIL
SPRAY WHOLE PLANT AND SOIL
SPRAY SEEDLINGS
SPRAY BASAL STEM OF PLANT
SPRAY UNDER PLANT CANOPY
SPRAY INTO SOIL BEFORE PLANTING
OR AFTER SOWING SEEDS
SPRAY AFTER SEEDING AND MULCHING
From Trichoderma
mixing tank
IRRIGATION FOR PADDY FIELD
Filtering
Adding Trichoderma
into nutrient solution
T100g/200L T200g/200L T100g/200L Control Control
(+Py) (+Py) (-Py) (+Py) (-Py)
CLEANING
DISEASE
LESION
PAINTING DISEASED LESIONS
(STEM ROT ON DURIAN TREE)
Trichoderma = 1 kg.
Fresh culture
Water = 2-4 l
Powder = 1 kg
DRIED
DISEASE
LESION
SUCCESSFUL CURING
SPRAYING DISEASE LESION
DRIED
DISEASE
LESION
SUCCESSFUL CURING
APPLICATION METHODS
BROADCASTING
SEED TREATMENT
SPRAYING
THROUGH IRRIGATION SYSTEM
HYDROPONIC SYSTEM
CURING
CAUTIONS
Mixing Trichoderma with old compost
Mixing Trichoderma with benomyl,
carbendazim and propiconazole is
prohibited
Spraying Trichoderma in the evening
SAWADEE