Must Know To Identify Your Machine Depends On What Section

1.
Must know to identify your machine depends on what section
HEMATOLOGY
1. Sysmex ST 4000I
2. MINDRAY BC 5180
- HEMATOLOGY ANALYZER - for CBC ( RBC ct, WBC ct. , WBC differential ct
( Neutrophils, lymphocyte, eosinophils, monocyte, basophils), hemoglobin,
hematocrit, platelet ct.)
- Violet top ( EDTA)
1. KC4 DELTA AND SYSMEX CA600 - for coagulation studies , APTT , PT
- Blue top, - sodium citrate
Test in hematology
ESR - BLACK TOP SODIUM CITRATE
2 METHODS - WESTERGREN AND WINTROBE
WESTERGREN TUBE OR WINTROBE TUBE IS PLACED ON THE
VACUTAINER TUBE, WAIT FOR 1 HR FOR THE RBC TO SETTLE DOWN,
RECORD THE VALUE AFTER 1 HR
YOU MUST ALSO KNOW THE NORMAL VALUES!
* REMEMBER : IF YOU USE TUBES WITH ANTICOAGULANT - LIQUID

PORTION IS CALLED PLASMA….
IF YOU USE PLAIN TUBE ----- SERUM
PBS - PERIPHERAL BLOOD SMEAR - FEATHERY EDGE
USE VIOLET TOP - EDTA
* REMEMBER THE APPEARANCE OF DIFFERENT CELLS ESPECIALLY
WBC( LOBES, GRANULES AND FUNCTION)
HCT AND HEMOGLOBIN - VIOLET TOP ; PLACE IN A HCT TUBE ,

CENTRIFUGE AND MEASURE
- MUST WIPE THE FIRST DROP OF BLOOD BECOZ IT IS CONTAMINATED
WITH TISSUE FLUID
COAGULATION STUDIES - PT AND APTT
REMEMBER THE REAGENTS USED

PT - THROMBOPLASTIN AND CALCIUM CHLORIDE + PATIENT SAMPLE
( PLASMA
APTT - PHOSPHOLIPIDS + ACTIVATORS ( SILICA OR ELLAGIC ACID)
REACTION END POINT : FORMATION OF CLOT OR FIBRIN CLOT

CM
4. SYSMEX UF 5001 - FLUORESCENCE FLOW CYTOMETRY
READ YOUR REPORTS FOR MORE INFORMATION

- ABLE TO DETECT UTI
CHEMISTRY
1. COBAS INTEGRA - WET CHEMISTRY

MOST CHEMISTRY TEST - RED TOP OR PLAIN TUBE
SAMPLE : SERUM
2. VITROS 250 - DRY CHEMISTRY

3. MINDRAY BS 200E - CHEMISTRY AND THERAPEUTIC DRUG MONITORING
AND SUBSTANCE ABUSE ( MARIJUANA, METAMPHETAMINE….)
● TAKE NOTE ALSO THE NUMBER OF TEST PER HR

BILIRUBIN - RED TOP COVERED WITH CARBON PAPER
TO AVOID EXPOSURE TO LIGHT
EXPOSURE TO LIGHT = DECREASE LEVEL
HBA1C - VIOLET TOP

REMEMBER THE NORMAL VALUE
- FOR MONITORING OF DIABETES; THE LEVEL OF HBA1C CORRESPOND
TO THE 3 MONTHS VALUE OF GLUCOSE IN THE BLOOD SINCE RBC HAS
A LIFESPAN OF 120 DAYS
4. OPTI CCA TS2 - BLOOD GAS , ELECTROLYTES, ACID BASE BALANCE,
IONIZE CALCIUM , GLUCOSE AND LACTATE
FOR BLOOD GAS : ARTERIAL BLOOD ( BRIGHT RED ) HEPARIN

( GREEN TUBE)
THE REST : USE RED TOP
- RUNS IN SINGLE CASSETE
- MEASURES ; OPTICAL FLUORESCENCE
5. MINICAP SEBIA FLEX PEIRCING
- AUTOMATED CAPILLARY ELECTROPHORESIS
- SAMPLE “ ; SERUM OR WHOLE BLOOD
- TEST : PROTEIN, IMMUNOTYPING , HBA1C, HEMOGLOBIN AND CDT
CARBOHYDRATE DEFFICIENT TRANSFERRIN
IMMUNOLOGY
1. ARCHITECT C 4000 - CLIN CHEM AND IMMUNOLOGY
- Hepatitis profile ( HBsAg, HBeAg, anti HBS ( antibody produce after
vaccination of hepa) , anti HBe)
- Cardiac markers - troponin I and troponin T, myoglobin
- Tumor markers ( AFP, alpha feto protein, CA125.. CEA, PSA prostate specific
antigen )
- Principle : ELISA enzyme linked immunosorbent assay
1. Architect I1000 SR - same
3. ALERE PIMA - FOR CD4 COUNT - important in cases of hiv
Lesser than 200 cd4 ct === AIDS
SAMPLE ; WHOLE BLOOD IN EDTA
4. EVOLIS - ELISA
5. BIORAD CODA - EIA MICROPLATE = ENZYME IMMUNOASSAY
HISTOPATH
LEICA TP 1020 AND STP 120 SPIN TISSUE PROCESSOR
- MACHINE FOR TISSUE PROCESSING
SPECIMEN ( ORGAN) -- > PATHOLOGIST WILL CUT AND MEASURE THE
ORGAN, CHOOSE THE SITE WHERE POSSIBLE DISEASE CAN BE
DETECTED , THE PATHOLOGIST WILL DESCRIBED THE APPEARANCE OF
THE ORGAN ----> MEDTECH WILL PROCESS THE TISSUE ---> FOR READING
OF PATHOLOGIST
TISSUE PROCESSING
1. FIXATION
2. DECALCIFICATION
3. DEHYDRATION
4. CLEARING
5. IMPREGNATION
6. EMBEDDING
7. MICROTOMY
8. STAINING
9. MOUNTING
1. FIXATION - AIM : TO PRESERVE ; TO HARDEN AND PROTECT
REAGENT : FORMALIN
2. DEHYDRATION - Removal of the fixative and water from the

tissue
- Tissue is subjected to INCREASING CONCENTRATION
OF ALCOHOL 70, 95, 100% ETHYL ALCOHOL
3. CLEARING - removal of alcohol
Reagent : xylene
4. IMPREGNATION AND EMBEDDING
Impregnation - removes clearing agents and replace it with paraffin wax to fill up
the spaces of the tissue
EMBEDDING - Tissue is place in a embedding medium and fill completely with
wax
Embedding media
1. Paraffin wax - most common
2. Celloidin (Collodion)
3. Gelatin
4. Plastic
5.Embedding molds:
■ Leuckhart’s Embedding Mold
■ Compound Embedding Unit
■ Plastic Embedding Rings and Base Mold
Tip : YOU MUST KNOW THE PICTURES OF ALL THE MACHINES
Manual Processing of Tissue Samples
Fixation:
10% Buffered Formalin 24 hours
Dehydration:
70% Alcohol 6 hours
95% Alcohol 12 hours
100% Alcohol 2 hours
100% Alcohol 1 hour
100% Alcohol 1 hour
Clearing:
Xylene or Toluene 1 hour
Xylene or Toluene 1 hour
Impregnation:
Paraffin Wax 15 mins
Embedding
READ ALSO STAIN LIKE PAPSMEAR STAIN PROCEDURE
BLOOD BANK
1. HISCL 5000
Principle : chemilumiscence enzyme immunoassay ( CLEIA)
2. GRIFOLS WADIANA - automated pre transfusion compatibility testing ( ABO,
RH, WEAK D, CROSSMATCHING, Antibody screening, DIRECT COOMBS)
using DG GEL CARDS
- ABO TYPING ( FORWARD AND REVERSE)
FORWARD TYPING
SAMPLE : WHOLE blood / PACKED RBC
REAGENTS : ANTI A (BLUE COLOR) ANTI B ( YELLOW)
RESULT : PRESENCE OF AGGLUTINATION ( POSITIVE)
Blood Reaction with Reaction

Group Anti-A with Anti-B
A + -
B - +
AB + +
0 - -
REVERSE TYPING - SAMPLE : PLASMA OR SERUM
REAGENTS : KNOWN A CELL AND KNOWN B CELL
1 DROP SAMPLE + KNOWN A
1 DROP SAMPLE + KNOWN B
Blood Reaction Reaction
Group with A with B
Cells cells
A - +
B + -
AB - -
O + +
RESULT : ABSENCE OF AGGLUTINATION (+)

3. Rh typing - detection of d antigen
Sample : whole blood

Reagent : anti D ( colorless)
Result : presence of agglutination +
COM TEC FRESENIUS kabi (BLOOD BANK)
- Apharesis machine ;
- Only cell of choice is being collected (PLATELETS), the other cells ( RBC
AND WBC, PLASMA) are flowed back to the body
Platelets - plateletpheresis
Plasma - plasmapheresis
MICROBIOLOGY
1. Vitek 2 compact - for bacterial identification and antimicrobial susceptibilty
testing
2. DL BT 112 - AUTOMATED BLOOD CULTURE SYSTEM
DETECTS WIDE RANGE OF MICROBES
- GROWTH OF BACTERIA = RESULT TO PRODUCTION OF CARBON
DIOXIDE, THE CARBON DIOXIDE WILL REACT TO WATER THAT WILL
PRODUCE HYDROGEN ION THAT WILL TURN THE COLOR OF THE
BOTTLE FROM BLUE TO ORANGE
THAT WILL CHANGE
COLOR IF BACTERIA IS
PRESENT
3. BD BA CTEC FX - BLOOD CULTURE INSTRUMENT
SAME AS NUMBER 2
AGAIN.. Read all your reports
Memorize gram stain and acid fast staining
How to coil sputum in AFB
In the collection of blood for culture collect abt 5- 10ml for adults

Must Know To Identify Your Machine Depends On What Section

Uploaded by

Document Informationclick to expand document information

Copyright:

Available Formats

Must Know To Identify Your Machine Depends On What Section

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Must Know To Identify Your Machine Depends On What Section

Uploaded by

Copyright:

Available Formats

1.

Must know to identify your machine depends on what section

* REMEMBER : IF YOU USE TUBES WITH ANTICOAGULANT - LIQUID

HCT AND HEMOGLOBIN - VIOLET TOP ; PLACE IN A HCT TUBE ,

REMEMBER THE REAGENTS USED

APTT - PHOSPHOLIPIDS + ACTIVATORS ( SILICA OR ELLAGIC ACID)

REACTION END POINT : FORMATION OF CLOT OR FIBRIN CLOT

READ YOUR REPORTS FOR MORE INFORMATION

1. COBAS INTEGRA - WET CHEMISTRY

2. VITROS 250 - DRY CHEMISTRY

● TAKE NOTE ALSO THE NUMBER OF TEST PER HR

HBA1C - VIOLET TOP

FOR BLOOD GAS : ARTERIAL BLOOD ( BRIGHT RED ) HEPARIN

2. DEHYDRATION - Removal of the fixative and water from the

4. IMPREGNATION AND EMBEDDING

Blood Reaction with Reaction

RESULT : ABSENCE OF AGGLUTINATION (+)

Sample : whole blood

Memorize gram stain and acid fast staining

How to coil sputum in AFB

You might also like