Development of Frog

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DEVELOPMENT OF

FROG
Presented By : Z-MY
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Gametogenesis
Structure of sperm
Egg of frog
Pseudocopulation
Unfertilized egg of frog
Steps of development
Cleavage in frog
Morulla
Blastula of frog
Gastrulation in frog
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Late gastrula
Late gastrula showing germ layers
Coelom formation in frog
Neurulation in frog
Metamorphosis in frog
• Development is in-direct in frog.
• Development is direct in all vertebrates except amphibia.
• Ovulation.
• Psuedocopulation.
• Oviposition.
• Insemination.
• Spawning.
• Fertilization.
Egg of frog
• Non-cleidoic
• Mesolecithal
• & telolecithal.
• A.P. contain female pronucleus, dark pigment & cytoplasm.
• V.P. Contain yolk, remain directed downward.
Pseudocopulation
• Male frog croaks during rainy night.
• Vocal sacks act as resonators.
• Male frog mounts on the back of female frog.
• Male clasps the female with copulatory pad.
• Amplexus.
• Oviposition:
• Female frog discharge 3000- 4000 eggs into the water.
• Insemination:
• Male discharge sperms on the eggs before reaching into the water.
• Fertilization: Fertilization is external taking place into the water.
• Sperms enter into the egg through any point in A. P.
• Cone of reception develop at the point where sperm touches the egg.
• Acrosome : +nt as cap on the head of Sperm.
• It secrets sperm lysin which dissolve hard cover of egg.
• Sperm enters into the egg with head & neck.
• 2nd Meiotic div takes place & 2nd polar body is formed.
• Grey crescent is formed in equitorial zone geometrically opposite to
entrance of sperm.
• Grey crescent marks the dorsal side of future embryo.
• Sperm entrance point marks anterior side of future embryo.
• Fertilization membrane is formed.
• Egg symmetry- before fertilization Radial.
• During fertilization Bilateral symmetry develop.
Cleavage
1st cleavage – meridional, holoblastic & eqaual. 2-blastomeres.
2nd cleavage – also meridional, holoblastic & eqaual. But perpedicular to 1st. 4-
blastomeres formed.
3rd cleavage – horizontal, holoblastic & unequal, just above the equator. 8-
blastomeres formed.
4th cleavage – two synchronous meridionial & Holoblastic. 16- blastomers formed .
5th cleavage – two simultaneous horizontal cleavage. 32- blastomeres formed..
After this micromeres divide more rapidly than megameres due to absence of
yolk..
Cleavage is indeterminate.
Blastula
• Blastocoel appear towards A.P.
• Blastula – coelablastula.
• Blastocoel contain albuminous fluid.
• Blastocoel start forming at 8-celled stage.
• Blastocoel is also called segmentation cavity.
• Uni-layered blastula.
Gastrula
Transformation of Single walled Blastula to double walled Gastrula
Differentiation of cells.
Formation of primary germ layers.
Formation of Blastopore.
Formation of Archenteron.
Formation of yolk plug.
Obliteration of Blastocoel.
Blastopore develops into future Anus.
Involves –
A) Epiboly
B) Invagination/ blastopore formation
C) Imboly /Emiboly/ involution/archenteron formation
D)Rotation of Gastrula.
E) Contraction of lips.

Ingression is migration of individual vegetal cells to the interior of embryo.


Organizer:
The dorsal lip of blastopore in the amphibian gastrula is called primary oraganiser.
The theory of Oraganiser(inductor) in amphbibia was introduced by Spemann in 1938. He
was awarded Nobel Prize for this work.
• Takes place after Gastrulation.
• Neural tube is formed.
• It includes formation of neural tube. Neural tube latter develop into
Brain & spinal cord.
• Notogenesis - formation of notochord.
Post neururation
• Takes place inside egg membrane upto tail bud larval stage.
• Hatching occurs at the 6th day of embryo life.
• Tadpole remain attached to aquatic plant by oral sucker.
• After 24 hrs of hatching mouth and anus are perforated.
Metamorphosis
• First sign of metamorphosis is appearance of hind-limbs.
• End of tadpole in the life history is marked by appearance of
forelimbs.
• Progressive morphological changes-
• formation of tongue and teeth.
• kidney pro-nephric to meso – nephric.
• 2-chambred heart changed to 3-chambers.
• Formation of forelimb & hind limb.
• Gills develop into lungs.
• Regressive-
• dis-appearance of sucker, gills, tail, lateral receptors.
• Intestine become smaller. The intestine shrinks to one-third to its original
length.
• Autolysis- Cathepsin , lysosomal enzyme help to reabsorb elongated
tail.
• Nervous system remain unchanged/least change.
• Respiratory system undergo maximum changes.
• Skin get cornified , Mucus and poison gland develop.
• Feeding habit: herbivorous to carnivorous.
• Discovered by Gudernatsch(1912)
• Thyroxin hormone. Hasten metamorphosis.
• Thiourea – anti thyroxin.
• Neoteny-
• Paedogenesis-
• 1)Archenteron in frog’s embryo is developed due to: (MOE 2069)
• A) Epiboly b) Emiboly
• c)Cleavage division d)Neurulation
• 2)Vegetal hemisphere of frog’s egg consists of: (MOE 2068)
• A)Gray crescent b)germinal vesicle
• C)Melanin d) yolk
• 3)The origin of frog’s brain is – (MOE 2069)
• A)Endodermal b) Mesodermal
• C) Ectodermal d) Endo-mesodermal
• 4)Type of cleavage in frog’s zygote is (MOE 2068)
• A) Holoblastic & equal b) Holoblastic & unequal
• C) Meroblastic d) Diploblastic
• 5)Change in sperm for fertilization is (BPKHIS 2012)
• A) capacitation b)insemination
• C)polygamy d)sterilization
• 6)The cavity that appears during embryogenesis of frog is called (MOE
2064)
• A)Mesenteron b)Blasocoel
• C)Coelenteron d) Archenteron
• 7)Yolk plug is seen in (IOM 1997)
• A) Blastula b) Gastrula
• C) Morula d) Cleavage
• 8)Which of the following is outer layer of an early embryo (MOE 2057)
• A)Endoderm b) endoderm
• C) ectoderm d)Mesoderm
• 9)Blastopore is present in _stage(BPKIHS 1994)
• A)Morula b)Blastula
• C) Gastrulad)Neurula
• 10) Germ layers are seen in (BPKIHS 1997)
• A) Gastrula b) Blastula
• C) Cleavage d) Fertilization
• 11) The tadpole of frog feeds upon (BPKIHS1997)
• A) zoo plankton b)swimming insect
• C) aquatic flora d)Smaller fishes
12)In frog, jelly around the eggs is deposited (BPKIHS 2005)
A)in water after fertilization
B) in water during fretilization
C) in oviduct
D) in the ovary
13) Blastopore is found in (IOM 1998)
A) Blastula & is the opening of blastocoel
B) Blastula & is the opening of archenteron
C)Gastrula & is the opening of blastocoel
D)Gastrula & is the opening of archenteron
• 14)Metamorphosis in frog can be accelerated by (BPKIHS 1995
• A) iodin b)Phospohorous
• C)Potassium d) Calcium
• 15) Which of the following is true about mesoderm ? (MOE 2002)
• A)It forms CNS
• B)it forms the pouches lining of the coelom
• C)it has no contribution to alimentary canal
• D) It does not form blood vessel
16) Blastopore in the vertebrates develop into (IOM 2010)
A)Anus b) Mouth
C)Notochord d)Both mouth and anus.
17) Metamorphosis in frog is regulated by the secretion of ( IOM 2010)
A) Pituitary b)Adrenal
C)Thyroxin d) Calcitonin
18)Skeleton & muscles in a vertebrate embryo develop from (IOM 2010)
A)Ectoderm & endoderm b)Ectoderm only.
C)Ectoderm & mesoderm d)Mesoderm only.
• 19) Vertebral column of vertebrates is originated from ( MOE 2010)
• A)Neural tube b) Notochord
• C)Ectodermal plate d)Mesodermal plate
• 20) In frog coelom is derived from (MOE 2010)
• A)Ectoderm b) Mesoderm
• C) Endoderm d) Neuroectoderm.
• 21) _____ gives origin to nervous system ( EP 2005)
• A) ectoderm b) mesoderm C) endoderm
• d)ectoderm & endoderm e)Endoderm & mesoderm
• 22) _____gives origin to skeletal system(PE 2005)
• A) ectoderm b) mesoderm C) endoderm
• d)ectoderm & endoderm e)Endoderm & mesoderm
• 23)segmentation is another name for
• A)cleavage b)Blastocoel
• c)Archenteron d)Body cavity.
• 24)Blastopore develops into mouth in
• A)frog b)rabbit c)Leech d)Human being
• 25)Gastrulation in frog involves
• A)epiboly b)emboly c)invagination d)all

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