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    Abo Reverse Grouping: Becios, Cabus, Del Rosario and Manalo

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    Marj Mendez
    This document provides instructions for performing an ABO reverse blood grouping test. The test involves mixing an unknown serum sample with known red blood cells of types A, B, AB, and O. Reagents needed include gloves, test tubes, a centrifuge, saline solution, and red blood cell suspensions. The procedure describes labeling test tubes and adding drops of serum and cells. The tubes are centrifuged, examined for agglutination or hemolysis, and the results are interpreted using a guide to determine the unknown serum type.

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    Abo Reverse Grouping: Becios, Cabus, Del Rosario and Manalo

    Uploaded by

    Marj Mendez
    22 views6 pages
    This document provides instructions for performing an ABO reverse blood grouping test. The test involves mixing an unknown serum sample with known red blood cells of types A, B, AB, and O. Reagents needed include gloves, test tubes, a centrifuge, saline solution, and red blood cell suspensions. The procedure describes labeling test tubes and adding drops of serum and cells. The tubes are centrifuged, examined for agglutination or hemolysis, and the results are interpreted using a guide to determine the unknown serum type.

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    ABO-REVERSE-GROUPING

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    This document provides instructions for performing an ABO reverse blood grouping test. The test involves mixing an unknown serum sample with known red blood cells of types A, B, AB, and O. Reagents needed include gloves, test tubes, a centrifuge, saline solution, and red blood cell suspensions. The procedure describes labeling test tubes and adding drops of serum and cells. The tubes are centrifuged, examined for agglutination or hemolysis, and the results are interpreted using a guide to determine the unknown serum type.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    22 views6 pages

    Abo Reverse Grouping: Becios, Cabus, Del Rosario and Manalo

    Uploaded by

    Marj Mendez
    This document provides instructions for performing an ABO reverse blood grouping test. The test involves mixing an unknown serum sample with known red blood cells of types A, B, AB, and O. Reagents needed include gloves, test tubes, a centrifuge, saline solution, and red blood cell suspensions. The procedure describes labeling test tubes and adding drops of serum and cells. The tubes are centrifuged, examined for agglutination or hemolysis, and the results are interpreted using a guide to determine the unknown serum type.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 6

    ABO REVERSE

    GROUPING

    Becios, Cabus, Del Rosario


    and Manalo
    Materials Needed

     Gloves  Test Tubes


     Marking Pen  Pasteur Pipette
     Gum Label  Test Tube Rack
     Centrifuge  Nescofilm
    Reagents/Samples

    – Unknown Sera
    – Known A, B, AB and O 2-5% red cell suspension
    – Normal Saline Solution (0.85-0.9% NaCl) in wash
    bottle
    Procedure

    1. Prepare a 2-5% red cell suspension of A, B, AB, and O cells.


    2. Label 4 wasserman test tubes as A, B, AB and O respectively.
    3. Follow the table below.
    CONTENT A B AB O
    Unknown Serum 2 drops 2 drops 2 drops 2 drops
    A-cell 1 drop - - -
    B-cell - 1 drop - -
    AB-cell - - 1 drop -
    O-cell - - - 1 drop
    Procedure

    4. Cover the test tube with Nescofilm.


    5. Mix gently and centrifuge for 15 seconds at 3,400 rpm.
    6. Gently dislodge the cell button and examine for
    hemolysis or agglutination.
    7. Grade each reaction and record the results.
    Guide in the Interpretation of
    Backward Blood Grouping
    Patient’s Serum Known A-cells Known B-cells Known AB-cells Known O-cells

    A o + + o

    B + o + o

    AB o o o o

    O + + + o

    (+) with agglutination (-) no agglutination

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