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    Gene Regulation of Prokaryotic Transcription

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    Ayu Tri Agustin
    This document summarizes gene regulation of prokaryotic transcription. It discusses how prokaryotes lack membrane-bound organelles and have DNA that is not separated from the cell. The stages of transcription - initiation, elongation, and termination - are described. Transcription factors that activate or repress transcription are also covered. A case study examines using bacterial transcription as a target for new antibacterial drugs, as transcription is an underutilized target compared to cell wall or translation targets. Currently approved drugs that target bacterial RNA polymerase, such as rifampin, are discussed.

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    Gene Regulation of Prokaryotic Transcription

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    Ayu Tri Agustin
    70 views17 pages
    This document summarizes gene regulation of prokaryotic transcription. It discusses how prokaryotes lack membrane-bound organelles and have DNA that is not separated from the cell. The stages of transcription - initiation, elongation, and termination - are described. Transcription factors that activate or repress transcription are also covered. A case study examines using bacterial transcription as a target for new antibacterial drugs, as transcription is an underutilized target compared to cell wall or translation targets. Currently approved drugs that target bacterial RNA polymerase, such as rifampin, are discussed.

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    PPT Kuliah Biologi Sel dan Molekuler

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    This document summarizes gene regulation of prokaryotic transcription. It discusses how prokaryotes lack membrane-bound organelles and have DNA that is not separated from the cell. The stages of transcription - initiation, elongation, and termination - are described. Transcription factors that activate or repress transcription are also covered. A case study examines using bacterial transcription as a target for new antibacterial drugs, as transcription is an underutilized target compared to cell wall or translation targets. Currently approved drugs that target bacterial RNA polymerase, such as rifampin, are discussed.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    70 views17 pages

    Gene Regulation of Prokaryotic Transcription

    Uploaded by

    Ayu Tri Agustin
    This document summarizes gene regulation of prokaryotic transcription. It discusses how prokaryotes lack membrane-bound organelles and have DNA that is not separated from the cell. The stages of transcription - initiation, elongation, and termination - are described. Transcription factors that activate or repress transcription are also covered. A case study examines using bacterial transcription as a target for new antibacterial drugs, as transcription is an underutilized target compared to cell wall or translation targets. Currently approved drugs that target bacterial RNA polymerase, such as rifampin, are discussed.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 17

    GENE REGULATION OF

    PROKARYOTIC TRANSCRIPTION

    Ayu Tri Agustin


    Gigih Setia Wibawa
    Deni Harmoko

    Biology Department
    Faculty of Mathematics and Natural Sciences
    Universitas Brawijaya
    Malang
    • Prokaryotes are single-
    celled organisms that
    are the earliest and
    most primitive forms of
    life on earth.
    • Prokaryotic cells lack
    organelles found in
    eukaryoitic cells such
    as mitochondria,
    endoplasmic reticuli, Figure 1.
    and Golgi complexes. prokaryotic cell
    structure
    • They have no true 
    nucleus as the DNA is
    not contained within a
    membrane or separated
    from the rest of the
    cell, but is coiled up in
    PROKARYOTE GENE STRUCTURE
    TRANSCRIPTION
    • Transcription is the process
    of making RNA from DNA
    template.
    • Several key factors are
    involved in this process,
    including DNA, transcription
    factors, RNA polymerase,
    and ATP.
    • Transcription has three
    stages: initiation,
    elongation, and termination.
    ure 2. Promoter and Terminator structure of prokaryote orga
    Figure 3.
    transcription
    mechanism of
    prokaryotic
    TRANSCRIPTION ACTIVATOR
    TRANSCRIPTION REPRESSOR
    CASE STUDY

    Bacterial Transcription as a
    Target for Antibacterial Drug
    Development
    Cong Ma, Xiao Yang, Peter J. Lewis
    • Infections are
    still the leading
    cause of death
    in developing
    world

    WHO, 2016
    • Antibiotic resistance represents a
    serious and growing problem in the
    treatment of bacterial infections
    • Selecting an antibiotic that will
    optimally treat an infection while
    minimizing adverse effects
    • Most clinically approved antibiotics target
    :
    - bacterial cell wall growth/integrity
    - translation
    - DNA replication/segregation

    • Transcription appears to be an
    underutilized target
    Antibiotics targeting bacterial RNAP on
    the market:
    • Rifamycin
    • Fidaxomicin/lipiarmicin
    Figure 4. RNAP structure and functional motif. (A-B) Side and front views &
    (B-C) Enlarged views from panels A and B
    RYFAMYCIN
    Rifampin (RIF), synthesized
    from rifamycin SV, is orally
    effective and has broad-
    spectrum activity against
    Gram-positive, Gram-negative,
    and especially mycobacterial
    SORANGICIN
    pathogens

    Sorangicin is a macrocyclic
    antibiotic isolated from
    Sorangium cellulosum
    Figure 5. Antibiotics that bind close to the active site. (A)
    Space-filled structure of T. thermophilus RNAP holoenzyme
    with core subunits & (B) Rifampin (RIF) (red) and sorangicin
    (SOR) (yellow) have overlapping binding sites
    THANKYOU

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