Antioxidant Activity of Ayosep Leaf Formulated As Tea: Vaccinium Myrtoides

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Antioxidant Activity of Ayosep

(Vaccinium myrtoides) Leaf


Formulated as Tea
• This study aims to determine and evaluate the antioxidant
activity of ayosep leaves formulated as tea

• Source of information that the ayosep leaves can be used as a


beverage to the community of Suyoc, Mankayan, Benguet

• Enable residents to have access to cheaper natural antioxidant


tea, acting as a basis for local product development
logy
Two milliliters each of the infused
Ayosep tea will be ayosep tea as the sample, ascorbic acid
placed in three as the positive control and water as the
erlenmeyer flasks negative control.

Mixtures will be shaken


vigorously and allowed to stand at
room temperature (20-25 ℃) for DPPH in methanol will be prepared
an hour. The cuvettes will be and two milliliters will be added to each
placed in the spectrophotometer cuvette containing the samples.
one after the other.
Preparation:

Weigh 3.3 grams of The solution will be


potassium boiled at 100℃ for 15 allowed to stand for 2
permanganate tol be minutes. The stopper will days, and then filtered
dissolved in 1000 ml be inserted in an with asbestos.
of water in a flask. erlenmeyer flask
containing the solution
Standardization and Assay
200 mg of sodium oxalate 7 mL of sulfuric acid will be Two milliliters of
will be weighed and dried added and will be subjected to the infused ayosep
in an oven at 110℃ until heat to about 70℃ for 15
leaves will be
minutes. Potassium
constant weight will be measured and
permanganate solution will be
obtained. Dried sodium placed in an
slowly added from the
oxalate will be dissolved in burette. Constantly stirring Erlenmeyer flask
250 mL of water. until a pale distinct pink color
will appear

infused ayosep leaves will be titrated using the


standardized potassium permanganate with constant
stirring until pale pink color appears.
Hydrogen Peroxide Test
A solution of 2 ml of the
hydrogen mixed with 3.4
tea
peroxide will mL phosphate
formulation
be prepared buffer and will
infused in
in a be added with
recently
phosphate 0.6 ml of
boiled
buffer (0.1M, hydrogen
distilled
pH 7.4). peroxide
water for 3
solution.
minutes

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