Rice Project

An intergraded approach on study of medicinal
properties of traditional pigmented rice varieties

of Tamil Nadu and evaluating anti-Breast cancer
properties
Submitted by : g.Nandhini
Guide : DR. R .Kalaivani
Class : ii msc. Biotechnology
Date : 08.04.19
INTRODUCTION: TRADITIONAL RICE
 Traditional rice varieties is also a big medicine .Rice is considered as a important food
in the Asian continent . Nearly 60% of the world population consumed rice as a stable
food.
 There where more than 3 lakhs of rice varieties are found in the India and 5000 rice
varities are founds in Tamil Nadu . Each rice varities contains the unique of their own
(Taste , Size , Texture ) .
 Different rice varieties are used for the different kinds of people (Ex : for diabetic
peoples , for pregnant ladies, for children's , old people , and youngster ) it used for treat
the various disease with help of natural benefits
 The benefits of traditional food are fading away without any trace due to the heavy
influence of western food . Besides that nutritional ,Medicinal benefits the by-products
of rice are equally important.
Breast Cancer
 Breast cancer is cancer that develops from breast tissue.
Breast cancer is the most common type of cancer in the UK
and all over India .
 Most women diagnosed with breast cancer are over 50, but
younger women can also get breast cancer. About one in
eight women are diagnosed with breast cancer during their
lifetime. There's a good chance of recovery if it's detected
in its early stages.
 BRCA1 and BRCA2 are the most responsible genes for the
breast cancer Breast cancer survival rates have increased,
and the number of deaths associated with this disease is
steadily declining, largely due to factors such as earlier
detection, it can be cured.
MEDICINAL RICE VARIETIES TYPES
 Kala Namak
 Tooyamali (Maapilai Samba)
 Kullakkar
 Seeraga Samba
 Poongar
 Kattuyanam
 Bamboo Rice
 Brown Rice ( unpounded milled rice)
 Kavuni
Sivappu Kavuni
Karuppu Kavuni
Scope of the study
The leading cancer sites in Chennai among the female were breast (26.1%) and in
the states of TamilNadu are affected through by (47%) and the mortality rate in
TamilNadu is 35.7 per 100,000 in the year of 2010. And the situation was tackled by
the TamilNadu Health system in 2005 by Rs 597.15 crore financial support received
from the World Bank for treatment .
With keeping this view, marching towards the sustainable health, searching for the
nutritional food, the present study is intended to assess the medicinal properties of
traditional pigmented rice varieties of Tamil Nadu and evaluating anti-breast
cancer properties.
Objectives
This investigation is carried out with following objectives,
To collect pigmented rice varieties from Musiri, Ariyalur and

Thiruthuraipoondi
To study the physiochemical properties and estimate the nutritional
values
To evaluate phytochemicals and profiling bioactive compounds in the
samples by GC- MS analysis
To assess antioxidant activity of the samples by DPPH method
To examine the effect of medicinal rice on anti-breast cancer by in vivo
method
Karuppu kavuni Rice
“American Chemical Society (ACS) says, one spoonful of Black rice contains
more anthocyanin antioxidant than a spoonful of Blue Berries”
 Black kavuni has 18 amino acids , zinc that counts for immunity iron that
counts for carrying oxygen and the list goes like copper, carotene, and
anthocyanin and several important vitamins
 Black rice helps to prevent cancer , diabetes , and heart disease. This is
very good for kidney ,stomach and liver .
 Black rice is the rich source of constituents such as protein, minerals,
vitamin, essential amino acid like lysine, tryptophan, vitamin such as
vitamin B1, vitaminB2, folic acid and minerals such as iron, zinc calcium,
phosphorus and selenium good source of fibre plant
Sivappu kavuni Rice
 The name sivappu kavuni was born through by the colour of the grain
kernel and otherwise called as cargo rice.
 It contains high amount of anthocyanin and has nutty taste.
 It contains the nutritive value of (45 gram) is 93% carbohydrate and
fibres, 20mg of calcium, 95 mg of potassium,2 grams of fibre and 3g
of protein, it is completely fat free .
 A gluten free grain and enhances the oxygen level, delaying of
ageing cells, prevent from cancer, antioxidant , detoxifying agent ,anti
inflammatory activity cure bone related problem and also helps in
boosting the healing properties
Brown Rice
 Brown rice is contains lot of health benefits it’s

considered as a World’s Healthiest food ,and it is a whole
grain it consist of three parts namely bran , germ and
endosperm and outer inedible hull is removed through by
the process
 Nutrient profile of the brown rice is low in calories , high in
fiber , gluten-free .USA Rice federation noticed that it
doesn’t contains Trans-fat or Cholesterol and it contain
trace amount of fat and sodium .
 It is good for the heart ,aids digestion , and reduce the
risk of cancer , diabetes and high cholesterol
RESULTS
BROWN RICE
S.NO TEST OF THE SAMPLE %
1 Moisture 9.6
2 Ash 1.7
3 Carbohydrate 69
4 protein 15.581
5 Fat 1.02
6 Fiber 0.95
7 FFA 3.0
8 Antioxidant activity 96
GCMS For Brown rice sample
Cyto toxic activity of Brown rice sample
Activity at the concentration of IC50 100.12µg/ml.

SIVAPPU KAVUNI
S.NO TEST OF THE SAMPLE %

1 Moisture 13.5
2 Ash 1.8
3 Carbohydrate 35
4 protein 8.9
5 Fat 1.50
6 Fiber 0.7
7 FFA 3.90
8 Antioxidant activity 98
GCMS for Sivappu Kavuni Rice Sample
Cytotoxic activity of sivappu kavuni Rice Sample
Activity at the concentration of IC50 117.43 µg/ml.

KARUPPU KAVUNI
S.NO TEST OF THE %

SAMPLE
1 Moisture 13.4
2 Ash 1.9
3 Carbohydrate 54
4 protein 7.27
5 Fat 1.75
6 Fiber 1.58
7 FFA 3.38
8 Antioxidant 92
activity
Cytotoxic activity of Karuppu
GCMS for Karuppu Kavuni Rice Sample
kavuni Rice Sample
Activity at the concentration

of IC50 129.1 µg/ml.
Conclusion
 The moisture content found in the present study is well within safe limits
and high percent carbohydrates content of the rice varieties signifies high
level of starch and its show that rice is a good source of energy .
 The rice protein is superior because of its unique composition of
essential amino acids. The highest amount of protein was found in Brown
Rice.
 This work has revealed that the Traditional rice contains some
properties to cure the breast cancer cell without any side effects .
 Due to the natural Antioxidant and Nutritional properties we don’t
want to add any artificial supplements to the diet
 Hence, the present study is intended to investigate the nutrients,
phytochemicals of pigmented traditional rice varieties of Tamil Nadu and
find a sustainable solution for Breast cancer for young women generations
towards smart health management. It works with underlining principle,”
Food as a Medicine ˝ .

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An intergraded approach on study of medicinal

properties of traditional pigmented rice varieties

Guide : DR. R .Kalaivani

Class : ii msc. Biotechnology

from the World Bank for treatment .

traditional pigmented rice varieties of Tamil Nadu and evaluating anti-breast

This investigation is carried out with following objectives,

To collect pigmented rice varieties from Musiri, Ariyalur and

 Brown rice is contains lot of health benefits it’s

S.NO TEST OF THE SAMPLE %

Cyto toxic activity of Brown rice sample

Activity at the concentration of IC50 100.12µg/ml.

S.NO TEST OF THE SAMPLE %

Activity at the concentration of IC50 117.43 µg/ml.

S.NO TEST OF THE %

Activity at the concentration

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