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    Enzyme: Oleh Yana Cahyana Stp.,Dea.,Ph.D

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    Rina Aryanti
    - Enzymes are protein catalysts that accelerate chemical reactions. They have high catalytic power and specificity, recognizing particular substrates. - Enzyme activity can be regulated by temperature, pH, substrate and enzyme concentration. Michaelis-Menten kinetics describe enzyme reactions using terms like Vmax and Km. - Enzymes can be inhibited reversibly by competitive, non-competitive or mixed inhibitors binding at the active site, or irreversibly through covalent bonds. Inhibition has applications like treating methanol poisoning.

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    Enzyme: Oleh Yana Cahyana Stp.,Dea.,Ph.D

    Uploaded by

    Rina Aryanti
    35 views21 pages
    - Enzymes are protein catalysts that accelerate chemical reactions. They have high catalytic power and specificity, recognizing particular substrates. - Enzyme activity can be regulated by temperature, pH, substrate and enzyme concentration. Michaelis-Menten kinetics describe enzyme reactions using terms like Vmax and Km. - Enzymes can be inhibited reversibly by competitive, non-competitive or mixed inhibitors binding at the active site, or irreversibly through covalent bonds. Inhibition has applications like treating methanol poisoning.

    Original Description:

    ini adalah file presentasi dari dosen mengeni enzim

    Original Title

    Enzim

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    - Enzymes are protein catalysts that accelerate chemical reactions. They have high catalytic power and specificity, recognizing particular substrates. - Enzyme activity can be regulated by temperature, pH, substrate and enzyme concentration. Michaelis-Menten kinetics describe enzyme reactions using terms like Vmax and Km. - Enzymes can be inhibited reversibly by competitive, non-competitive or mixed inhibitors binding at the active site, or irreversibly through covalent bonds. Inhibition has applications like treating methanol poisoning.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    35 views21 pages

    Enzyme: Oleh Yana Cahyana Stp.,Dea.,Ph.D

    Uploaded by

    Rina Aryanti
    - Enzymes are protein catalysts that accelerate chemical reactions. They have high catalytic power and specificity, recognizing particular substrates. - Enzyme activity can be regulated by temperature, pH, substrate and enzyme concentration. Michaelis-Menten kinetics describe enzyme reactions using terms like Vmax and Km. - Enzymes can be inhibited reversibly by competitive, non-competitive or mixed inhibitors binding at the active site, or irreversibly through covalent bonds. Inhibition has applications like treating methanol poisoning.

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    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 21

    ENZYME

    OLEH
    YANA CAHYANA STP.,DEA.,Ph.D
    Kuliah biokim TIP

    Outline
    - Introduction
    - Catalytic power, specificity, &
    regulation
    - Kinetics of enzyme-catalysed
    reaction
    - Enzyme inhibition
    Kuliah biokim TIP

    Introduction
    Enzymes are fundamental to biochemistry, the food
    sciences and biotechnology

    Much of biotechnology is concerned either with


    producing or using enzymes

    Applications are in a wide range of sectors particularly the food industry

    Any practising biotechnologist, biochemist or food


    scientist will work with enzymes at some point
    Knowledge of the fundamentals of enzymology is
    important
    Kuliah biokim TIP

    Nama :

    Introduction

    -ase : invertase, triosa fosfat dehidrogenase


    -in : pepsin, chymotrypsin

    Lisozim?

    Kuliah biokim TIP

    Introduction

    Karakteristik enzim
    -Katalisator reaksi
    -Mengubah substrat jadi produk
    -Protein
    -Bekerja spesifik
    -Berat molekul (BM) enzim>>>> (BM) substrat
    -Mempunyai gugus dan ruang aktif
    -Mempunyai bagian non protein
    -Kompleks E-S merubah bentuk ruang aktif dan substrat

    Kuliah biokim TIP

    Introduction
    Enzim bekerja dengan menurunkan energi aktifasi

    Kuliah biokim TIP

    Catalytic power, specificity, &


    regulation
    - Accelerating reaction rate as much as 1016 over
    uncatalysed levels
    ex urease catalyses the hydrolysis of urea :

    Catalysed reaction : 3 x104/sec


    Uncatalysed one : 3 x 10-10/sec
    Catalytic power of urease =1014

    Kuliah biokim TIP

    Catalytic power, specificity, &


    regulation
    -Molecular
    recognition
    based
    on
    structural
    complementarity
    -Specific site on the enzyme where substrate binds and
    catalysis occurrs is called the active site
    -Some enzymes rely solely on their amino acid residues,
    others require cofactor either metal ions (Fe2+,Fe3+, Mg2+,
    Cu2+, Mg2+, Se) or organic molecules (coenzyme, ex:
    vitamin).

    Kuliah biokim TIP

    Enzyme Kinetics
    -Pengaruh suhu pada aktifitas enzim

    -Pengaruh pH pada aktifitas enzim

    Kuliah biokim TIP

    Enzyme Kinetics
    -Pengaruh konsentrasi enzim

    -Pengaruh konsentrasi substrat

    Kuliah biokim TIP

    Enzyme Kinetics
    -Michaelis-Menten Equation

    v= kecepatan reaksi enzimatis, Vmax=kecepatan maksimum


    Kmax=konstanta Michaelis-Menten, [S]=konsentrasi substrat
    Kuliah biokim TIP

    Enzyme Kinetics
    Konstanta Michaelis-Menten (Km)
    -Salah satu ciri enzim
    -Menggambarkan afinitas enzim terhadap substrat
    Km

    afinitas

    -Km <10-6M sangat penting dalam metabolisme,


    Km >10-2M dianggap kurang penting
    -Kerja penghambatan enzim

    Nilai Km
    Kuliah biokim TIP

    Konstanta Michaelis-Menten (Km)

    Kuliah biokim TIP

    Enzyme Kinetics
    Transformation of Michaelis-Menten equation :
    Lineweaver-burk double reciprocal plot

    Kuliah biokim TIP

    Enzyme Unite
    -Aktifitas enzim= banyaknya substrat yang dirubah menjadi produk
    oleh sejumlah enzim
    -Aktifitas enzim dinyatakan dalam unit (U)
    1 unit= jumlah enzim yg dapat mengkatalisis 1 mol substrat per
    menit pada kondisi tertentu
    Specific activity =

    Enzyme activity (U)


    Amount of protein present (mg)

    Cellulase
    -Amylase
    Polygalacturonase
    Glucoamylase
    -1,3-Glucanase
    Protease

    1000 U/g
    200 U/g
    280 U/g
    570 U/g
    1970 U/g
    2000 U/g
    Kuliah biokim TIP

    Enzyme Inhibition
    Many compounds can inhibit enzymes
    Highly reactive chemicals can form covalent
    bonds with reactive groups on the enzyme and
    irreversibly inhibit the enzyme
    Other molecules interact with the enzyme in
    reversible ways to produce inhibition

    Kuliah biokim TIP

    Enzyme Inhibition

    Reversible
    (Ikatan non kovalen)

    Irreversible
    (Ikatan kovalen)

    -Kompetitif
    -Non kompetitif
    - Kombinasi

    Kuliah biokim TIP

    Enzyme Inhibition
    Penghambatan Kompetitif
    -Inhibitor bersaing dengan substrat
    memperebutkan ruang aktif yang
    sama

    Contoh:
    Pemberian etanol bagi yang keracunan
    metanol

    Kuliah biokim TIP

    Enzyme Inhibition
    Penghambatan Non-Kompetitif
    inhibitors bind to the enzyme-substrate complex and block the catalytic step

    Kuliah biokim TIP

    Enzyme Inhibition
    Penghambatan Kombinasi (Campuran)

    Kuliah biokim TIP

    Enzyme Inhibition
    Penghambatan Irreversible
    Penicilin menghambat glycoprotein peptidase
    dalam croslinking peptidoglikan untuk sintesa
    dinding sel

    Kuliah biokim TIP

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