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    Immunologic Study of Streptolysin

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    Aishwarya Bharath
    Streptolysin is a streptococcal exotoxin produced by Streptococcus pyogenes that exists in two types: streptolysin O (SLO) and streptolysin S (SLS). SLO is oxygen-labile and causes lysis of red blood cells and cardiac issues. The host body produces antibodies to SLO, known as antistreptolysin O (ASO), in response to infection. The ASO titer test qualitatively measures ASO antibodies in serum and an elevated titer above 200 indicates a recent streptococcal infection. The test works by detecting agglutination of latex particles coated with streptococcal exotoxins in the presence of ASO antibodies

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    Immunologic Study of Streptolysin

    Uploaded by

    Aishwarya Bharath
    220 views12 pages
    Streptolysin is a streptococcal exotoxin produced by Streptococcus pyogenes that exists in two types: streptolysin O (SLO) and streptolysin S (SLS). SLO is oxygen-labile and causes lysis of red blood cells and cardiac issues. The host body produces antibodies to SLO, known as antistreptolysin O (ASO), in response to infection. The ASO titer test qualitatively measures ASO antibodies in serum and an elevated titer above 200 indicates a recent streptococcal infection. The test works by detecting agglutination of latex particles coated with streptococcal exotoxins in the presence of ASO antibodies

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    Streptolysin is a streptococcal exotoxin produced by Streptococcus pyogenes that exists in two types: streptolysin O (SLO) and streptolysin S (SLS). SLO is oxygen-labile and causes lysis of red blood cells and cardiac issues. The host body produces antibodies to SLO, known as antistreptolysin O (ASO), in response to infection. The ASO titer test qualitatively measures ASO antibodies in serum and an elevated titer above 200 indicates a recent streptococcal infection. The test works by detecting agglutination of latex particles coated with streptococcal exotoxins in the presence of ASO antibodies

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    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    220 views12 pages

    Immunologic Study of Streptolysin

    Uploaded by

    Aishwarya Bharath
    Streptolysin is a streptococcal exotoxin produced by Streptococcus pyogenes that exists in two types: streptolysin O (SLO) and streptolysin S (SLS). SLO is oxygen-labile and causes lysis of red blood cells and cardiac issues. The host body produces antibodies to SLO, known as antistreptolysin O (ASO), in response to infection. The ASO titer test qualitatively measures ASO antibodies in serum and an elevated titer above 200 indicates a recent streptococcal infection. The test works by detecting agglutination of latex particles coated with streptococcal exotoxins in the presence of ASO antibodies

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 12

    IMMUNOLOGIC STUDY OF

    STREPTOLYSIN

    STREPTOLYSIN
    Streptolysinis
    astreptococcalhemolytic exotoxin
    produced by Streptococcus
    pyogenes.
    There are two types:
    1. streptolysin O (SLO), which is
    oxygen-labile
    2. streptolysin S (SLS), which is
    oxygen-stable.

    Streptolysin O
    Anantigeniccompound produced by and isolated from
    groups A, C and G ofstreptococci. This cytolysin is
    capable of causinglysisin animalcellsespeciallyRBCs.
    It is alsocardiotoxic. It causes changes in
    myocardialcontractilityand affects the
    cardiacconduction system.
    It results in the formation of a specific antibody called
    antistreptolysin O or ASO by the host that prevents
    hemolysis.

    Ablood test, known as the antistreptolysinOtitre, is sometimes used to test


    for the antibodies.

    People who have had a streptococcus infection


    in the last few months may still have the
    antibody anti-streptolysinOin their blood.
    People who have an active streptococcus
    infection may also have the presence of this
    antibody in the blood.

    Thistitrehas a significance only if it is greatly


    elevated (>200).
    The antibody levels begin to rise after 1 to 3
    weeks of strep infection, peaks in 3 to 5 weeks
    and falls back to insignificant levels in 6 months.
    High ASO titer is seen in rheumatic fever,
    scarlet fever, glomerulonephritis, bechets
    disease

    ASO TEST
    The ASO latex test kit serology kit is
    intended for the qualitative and
    semi-quantitative measurement of
    antibodies to streptococcal
    exoenzymes in human serum.

    PRINCIPLE
    ASO test method is based on an
    immunological reaction between
    streptococcal exoenzymes bound to
    biologically inert latex particles and
    streptococcal antibodies in the test sample.
    The reagent has been adjusted in the way
    that presence of an ASO titer of 200 IU/mL or
    higher in the serum gives a visible
    agglutination of the latex particles without
    previous sample dilution.

    Procedure for qualitative


    test
    1. Bring the latex controls and serum sample
    to room temperature.
    2. Place 1 drop(50 microL) of ASO positive
    control on field 1 of reaction slide.
    3. Place 1 drop(50 microL) of ASO negative
    control on field 2 of reaction slide
    4. Use pipette/stir stick to deliver 1 drop of
    undiluted test serum sample to field 3.
    5. Gently suspend the AO latex reagent and
    add 1 drop to each test field.

    Normal Results
    A negative test result means you likely have not had a recent strept
    infection.Sometimes, a test that was negative the first time will be
    positive (meaning it detects ASO antibodies) when it is done again.

    Abnormal Results Mean


    An abnormal or positive test result means you recently had a strep
    infection, even if you had no symptoms. The ASO test may stay
    positive (sometimes called detectable) for 2 to 4 months after
    youwere first infected.
    It is shown by agglutination

    RESULT

    POSITIVE

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