Nfpa 45

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The American University in

Cairo
NFPA 45
FIRE PROTECTION FOR
LABORATORIES USING
CHEMICALS
Purpose of the Standard
This standard provides basic requirements
for the protection of life and property in
laboratory work where hazardous chemicals
are handled through prevention and controls
of fires and explosions.
To protect personnel from the effects of
toxic, corrosive, or otherwise hazardous
chemicals to which they may exposed as a
result of fire or explosion.
Definitions
Educational Occupancies:
Building used for the gathering of 6 or more persons for
the purposes of instruction, such as schools, universities,
colleges, and academies.
Laboratory Unit:
An enclosed space used for experiments or tests. It may
contain one or more separate laboratory work areas.A
laboratory unit is classified as A, B, or C according to the
quantities of flammable and combustible liquids.
Definitions
Laboratory Work Area:
A room or space for testing, analysis, research, instruction,
or similar activities that involve the use of chemicals. This
work area may or may not be enclosed.
Hazardous Chemicals:
A chemical with one or more of the following hazard
ratings as defined in NFPA 704, Standard system for the
identification of fire hazards of materials. Health-2,3, or 4;
Flammability 3 or 4; Reactivity 2, 3, or 4.
Laboratory Unit Fire Hazard
Classification
Excl.
quant. In
cabinets
and cans
Excl.
quant. In
cabinets
and cans
Incl..
quant. In
cabinets
and cans
Incl..
quant. In
cabinets
and cans
Lab. Unit
Class
Flam. Or
Comb.
Class
Unsprink Sprink. Unsprink Sprink.
Class A
High
Hazard
I
I, II, and
IIIA
300 gal.
400 gal.
600 gal.
800 gal

600 gal.
800 gal.
1200 gal.
1600 gal.
Class B
Interm.
Hazard
I
I, II, and
IIIA
150 gal
200 gal.
300 gal.
400 gal.
300 gal.
400 gal.
600 gal.
800 gal.
Class C
Low
Hazard
I
I, II, and
IIIA
75 gal.
100 gal.
150 gal.
200 gal.
150 gal.
200 gal.
300 gal.
400 gal.
Explosion Hazard Classification
A laboratory work area shall be considered
to contain an explosion hazard if:
1- Storage of materials with a reactivity hazard rating of 4.
2- Use or formation of materials with reactivity hazard
rating of 4.
3- Highly exothermic reactions such as polymerizations,
oxidations, nitrations, peroxidation, hydrogenation, or
organo-metalic reactions.
Construction Notes
Laboratories will be classified (NFPA 101)
as : Business Health Care, Industrial
Compartmentation of each laboratory unit is
achieved by providing: (One-hour rated
separation from adjacent labs or other areas.
Self-closing fire doors with at least
hour rating.
Two or more exits must be provided for
labs of 200 feet area or more.
Continue
Doors to corridors from laboratories must
swing in the direction of exit.
When central supply of flammable,
combustible or oxidizing gases is provided,
shut-off valves in accessible locations must
be provided, in addition to those at the
points of supply and use.
Continue
Controls for air, gas, and other utilities must
be color coded and labeled.
GFCI on electrical outlets within 6 feet of
all sinks.

Laboratory Unit Layout
Fire Protection
Portable fire extinguishers
Fire alarm systems
Evacuation and emergency plans.
Automatic extinguishing systems (sprinkler
system).
Inside standpipe and hose systems.
Lab Ventilating Systems
Haz. Chemicals shall not be recirculated.
Location of fresh air intakes avoid
drawing of hazardous chemicals from labs.
Negative pressure in the labs relative to the
corridors or adjacent non-labs areas.
Location of air supply diffusion devices to
avoid air current (hoods, exhaust system,
and fire detection or ext. systems)
Continue
Min. 6 air change per hour fume hoods.
Open use of hazardous chemicals 10 or
more air change per hour.
Exhaust ducts negative pressure.
Hoods air velocity : 80 100 fpm
Local exhaust systems (elephant trunks or
snorkels.
Continue
Exhaust ducts to outside the building
roofs 10 feet above roof level.
Lab hoods shall be located in areas of
minimum air turbulence.
Lab hoods shall not be located adjacent to a
single means of access to an exit or high
traffic areas.
Chemical Storage & Disposal
Hazardous chemicals minimum necessary
Segregation of incompatible materials.
Dating of the first opening 6 months
testing and evaluation and disposal.
Class I transference bonding and
grounding.
Classification of chemical wastes.
No disposal of chemical wastes to the sink.
Maximum Allowable Container
Capacity
Flammable
Liquids
Flammable
Liquids
Flammable
Liquids
Combustible
Liquids
Combustible
Liquids
Container
Types
IA IB IC II IIIA
Glass
Metal
Safety Cans
Metal Drums
1 pt
1 gal
2 gal
N/A
1 pt
5 gal
5 gal
5 gal.
1 gal
5 gal
5 gal
5 gal
1 gal
5 gal
5 gal
60 gal
5 gal
5 gal
5 gal
60 gal
Compressed and Liquefied Gases
Manual shutoff valves at each point of
supply and each point of use.
Permanent piping shall be identified with
the name of material.
Cylinders of gases having health hazard
rating of 3 or 4 shall be kept in ventilated
hood or other continuously mech.
Ventilated enclosure.
Maximum quantity and size
limitations for comp. Gas
cylinders
Flamm.
Gases and/or
oxygen
Flamm.
Gases and/or
oxygen

Liquefied
flamm. gases
Liquefied
flamm. gases
Gases with
health
hazards
ratings of 3
or 4

Sprinklered
Space
Unsprinkl.
Space
Sprinklered
Space

Unsprinkl.
Space

Max. # of
cyl. Per 500
ft or less
Max.
Cylnder Size
(inches)
6


10 X 50
3


10 X 50
3


9 X 30
2


9 X 30
3


4 X 15
Lab Operations and Apparatus
Automatic shutdown unattended lab
operations.
Refrigerators or freezers for flam.
Substances: Class I, Division 1 locations
inside the ref.
Outside the ref. : Class I, Division 2.
Over-temp shutoff switches for electrically
heated equipment.

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