PPV SATYA PRASAD Ph.D.

RESEARCH SCIENTIST
CSIR-Centre for Cellular and Molecular Biology,
Satellite Centre - Andhra Pradesh,
Govt. Siddhartha Medical College, Vijayawada,
Andhra Pradesh, India -520008.
E-mail: [email protected] Phone: +91 9398118847
In: www.linkedin.com/in/satya-prasad-ppv-86246680 +91 9392303477

SUMMARY:

A dedicated and fervent Research scientist with expertise in Molecular Biology, Microbiology, cancer biology,
Biochemistry, Bioinformatics, and Biostatistics. Demonstrates a track record of achieving desired outcomes. Utilizes
critical thinking in experimental design and data analysis to uncover novel discoveries. Records observations
meticulously, ensuring accuracy and maintaining a sterile and well-organized work setting. Proficient in adhering to
standard regulations and collaborating seamlessly with multidisciplinary teams to execute laboratory operations
proficiently.

EDUCATION:

Ph.D.: (Cancer Biology) 2022

National Institute of Nutrition (ICMR) Hyderabad / Registration at Krishna University, Machilipatnam,
India.
Title of Thesis: “Dietary Phytate-Iron Interaction Role on Suppressing Colon Cancer in Fisher 344 rats”.
Project Name: Dietary Phytate-Mineral Interaction Role on Suppressing Colon Cancer”.
Project Code: Project # IT PR3391/FNS/20/528/2011 (DBT) (National Institute of Nutrition (ICMR) &
Krishna University).
Post-Graduation: (M.Sc. Bio-Technology) 2010
Andhra Loyola College, Vijayawada, Andhra Pradesh, India.
Graduation: (B.Sc. (BZC)) 2008
YVNR Government Degree College, Kaikalur, Andhra Pradesh, India.

PROFESSIONAL EXPERIENCE 13 Years

Research Scientist: Apr 2021 - Present

CSIR-Centre for Cellular and Molecular Biology,
Satellite Centre of Andhra Pradesh State, Vijayawada,
Siddhartha Medical College, Andhra Pradesh, India.

Responsibilities:

 Design, conduct, analyze, and interpret scientific experiments. Formulating work plans, collaborating with
team members, evaluating test results, and implementing improvements while maintaining confidentiality.
 Conduct investigative studies involving contagious viral pathogens and handling of BSL-2 or BSL-3
 DNA and RNA extraction, cDNA synthesis, primers design, order, PCR optimization, and amplification,
NGS libraries preparation, barcoding, and vigilant monitoring for potential cross-contamination
occurrences.
 Oxford Nanopore Technology MK1C and Illumina Mi-seq Next Generation Technologies are currently
employed for comprehensive COVID-19 genome sequencing, analysis, data acquisition, and
documentation.
 Download and Install required programs Linux (Ubuntu), anaconda, rampart etc.
  Basic Computing Linux (Ubuntu), Running Artic-Rampart, SRA, and FastQC, Quality Control of Data
Depth coverage computation, Adapter trimming using Porechop, SAM, and Bam, and BCF File Indexing
and Sorting, Alignment with using Minimap2, Consensus Generation, Variant calling, Next Clade and
pangolin for identify clade and lineage.
 Preparing metadata in a required manner to submit GISAID, INSACOG, IBDC, and IHIP portals.
 Open communication with colleagues, prepare technical reports, and present data at regular group meetings
of IBDC, IHIP, and NSACOG India.
 Administer laboratory operations which includes ordering, inventories, and maintaining laboratory
equipment and consumables.
 Procurement according to prevailing procurement policies.
 Equipment management includes qualification, preventive maintenance, and repair of equipment as per lab
SOP, including ensuring compliance with safety codes and licenses.
 Monitoring and maintenance of chemical list through effective communication with different stakeholders
and external parties.
 Execute project tasks as a laboratory research Scientist alongside the project team.

Research Scientist: Sep 2019 - Apr 2021

Project Name: Virus Research and Diagnostic Laboratory (VRDL).
Sponsored by DHR/ICMR, Dept. of Microbiology,
Osmania Medical College, Hyderabad, INDIA.

Responsibilities:

 Collaborate with patient cohorts and pertinent models, encompassing both in vitro and in vivo, to study
infectious diseases.
 Plan, schedule, coordinated, and lead multiple MD Microbiology academic and ICMR research projects.
 Regular communication with principal investigators, collaborating with colleagues, and lab members for
the projects plan, implementation, evaluation, and progress reports.
 Perform MD Microbiology projects, technical evaluation, and progress hold division meetings, and be
involved in operations management meetings with ICMR and DHR.
 Project progress reports, evaluation, and planning are given by the HOD, principal investigator, and
principal.
 Develop protocols, SOP preparation, execute report for verification tests
 HCV Viral Load and Genotyping: Qualitative and Quantitative and LPA (Line Probe Assay)
 Dengue Chikungunya co-infection studies, Dengue Serotyping: Qualitative Real-Time PCR and
Qualitative Conventional PCR.
 Molecular and protein assays, such as PCR and ELISA, are utilized for the diagnosis of Measles, JE, CMV,
HSV1, HSV2, and Rubella.
 I served as the chief scientist and was responsible for overseeing the laboratory operations involved in
COVID-19 research and diagnostics, during the COVID-19 pandemic, in 2020.
 COVID-19 diagnosis training was offered and COVID-19 EQAS was conducted for the COVID-19 testing
staff at all medical colleges in Telangana State.
  Lab ICMR Portal, contingency, consumables, ICMR Auditing, PFMS, NIE Data Portal, operating shifts,
and biomedical waste management
 Development of quality management process plan and Developing protocols, execution, and reporting for
verification test
 Validation of diagnostics workflow and execution of diagnostic tests in clinical lab
 Evaluation of Commercial Feasibility on Individual Kit Components

Research Assistant: Oct 2015 - Sep 2019

Project Name: Virus Research and Diagnostic Laboratory (VRDL).
Sponsored by DHR/ICMR, Govt. Siddhartha Medical College, Vijayawada, India.

Responsibilities:

 Conducted various investigations on viral diseases using molecular and immunological techniques,
including handling of viruses and aseptic techniques.
 Proficient in DNA and RNA isolation, quantification, cDNA synthesis, primer and probe reconstitution,
and PCR and Real-Time PCR for qualitative and quantitative analysis.
 Experienced in performing Influenza panel PCR for detecting Inf A, Inf B, and RnaseP, as well as second-
round PCR for identifying H1N1, H5N1, H3N2, and H7N9.
 Skilled in preparing buffers such as PBS, TAE, and TBE, as well as conducting electrophoresis, gel
documentation, and result analysis.
 Knowledgeable in diagnosing viral diseases including Hepatitis A, Hepatitis E, Hepatitis B, Hepatitis C,
Measles, Mumps, Rubella, Cytomegalovirus, and EBV.
 Well-versed in internal auditing and implementing Quality Management Systems as per ISO 15189:2012
(NABL) standards.
 Proficient in laboratory operations and administration, including Medical Devices Quality Management,
maintaining log books, and health cards, and managing biomedical waste.
 Experienced in managing quality control and various aspects of sample collection, transport, packing,
storage, sample processing, pre-analytical and post-analytical procedures, as well as biomedical waste
management.
 Skilled in developing research protocols, manuscripts, presentations, and project reports, as well as
conducting outbreak investigations and maintaining relevant documents.
 Capable of utilizing BD FACS Calibur for flow cytometry analysis to determine CD4 cell count in
individuals with advanced HIV.
 Knowledgeable in a wide range of general Microbiology techniques, including culturing, detection, drug
sensitivity testing, and culture analysis.
 Proficient in TB liquid culture and LPA techniques, which are valuable for genotyping and identification
purposes in Microbiology.
Research Scholar/ Research Assistant: Apr 2012 - Sep 2015
Project Name: Dietary Phytate-Mineral Interaction Role on SuppressingColon Cancer”. (DBT).
National Institute of Nutrition/IGHGD Hyderabad, India.

Responsibilities:

 Proficient handling skills with laboratory animals such as rats and mice, including climate control, bedding,
cleaning, sterilization, and waste disposal.
 The Fisher 344 rats were administered azoxymethane dosage to induce tumor development.
 Preparation of AIN-93 phytate-supplemented powdered diet, along with monitoring weight and food intake.
 Blood samples are collected using orbital sinus and tail snip techniques.
 Animals are euthanized through CO2 asphyxiation, and various organs such as the colon, ileum, jejunum,
duodenum, liver, spleen, bone, brain, and eyeballs are collected.
 Expertise in histopathology tissue processing, embedding, sectioning, and H&E staining is utilized to
differentiate basic tissues.
 Methylene blue whole mount staining is employed to identify abnormal crypt foci in tissues.
 The stain Alcian Blue is used to visualize mucins in tissues.
 Perls' Prussian blue (PPB) staining is utilized to identify iron in tissues.
 Dithizone (DTZ) staining is employed for detecting zinc in tissues.
 Experience in immunohistochemical staining processes and interpretation of staining.
 TUNEL kit assay is used for detecting cell death in paraffin-embedded tissue sections.
 Levels of cell proliferation markers β-Catenin and PCNA are monitored in colonic tissue sections and
western blots.
 Levels of cell differentiation markers Lectin, sulpho, and sialo mucins are also monitored in colonic tissues
using Immunohistochemistry, western blots, and flow cytometry.
 Cell cycle dynamics during tumor induction are studied.
 Cell death detection (TUNEL assay, DNA fragmentation pattern), cell proliferation markers (PCNA, beta-
catenin), and cell differentiation markers (Lectin, sulpho, and sialo mucins) are analyzed.
 Iron and zinc levels were examined in tumor-induced rats, specifically in the tumor and normal colon tissue,
as well as in the liver and plasma.
 The study aimed to understand the underlying biochemical and molecular mechanisms that contribute to
the altered nutritional status of these trace elements.
 Various techniques were employed, including immunohistochemical and immunoblots analysis, RAS-
Mutations, and Reverse Transcription–Polymerase Chain Reaction to determine the expression pattern of
mineral transporters such as DMT1, Dcytb, IREG1, ferritin, transferrin receptor, ZnT1, ZnT4, and
metallothionein.
 Furthermore, the redox status of the colonic epithelial tissue was evaluated by measuring lipid and protein
oxidation.
Technical Assistant: Oct 2010 - Mar 2012
Project Name: High through Put Allele Determination in Pigeon Pea Plant(DBT).
Acharya NG Ranga Agriculture University, Hyderabad, India.

Responsibilities:

 The process of molecular breeding for the pigeon pea plant involves the development of varieties that
possess both disease resistance and high yield potential. This is accomplished through the implementation
of various techniques, including effective greenhouse management, precise pollination methods, and the
identification of polymorphic parents.
 To extract DNA, the c-tab method is utilized, followed by DNA quantification using the Nano-drop
technique. PCR (RFLP) is then conducted using SSRs (simple sequence repeat) primers and ILP (Intron
Linked Polymorphic) primers. A total of 600 primers have been standardized for this specific purpose.
 Agarose and Metaphor Agarose gel electrophoresis are employed to separate DNA fragments, while gel
documentation is used to visualize and analyze the obtained results.
 To identify polymorphic primers, Sanger's sequencing is performed using the ABI 3130xl instrument
equipped with 16 capillaries. This sequencing method plays a crucial role in determining the genetic
variations present within the pigeon pea plant.

TECHNICAL SKILLS &COMPETENCIES:

 Skilled in the areas of Cancer Biology, Molecular Biology, Biochemistry, Immunology, Microbiology, Bio-
Informatics, and Bio-Statistics.
 Proficient in various laboratory techniques such as Electrophoresis, Spectroscopy, Microscopy,
Centrifugation, and Chromatography.
 Experienced in handling infection samples, implementing Bio-safety procedures, and working with BSL-2
and BSL-3.
 Familiar with Next Generation Sequencing (ONT MK1C and Illumina MiSeq), Sanger sequencing, PCR,
Real-Time PCR, PCR optimization, DNA fragmentation, and DNA and RNA extraction (manual and
automated) quantification.
 Skilled in Bio-informatics pipeline, Next Generation Sequencing data analysis, data interpretation, and
troubleshooting. proficiency with using Linux and BASH. Also have knowledge onFASTQC, NCBI, SRA,
and basic Python and R.
 Proficient in experimental animal studies (Rats and Mice), Histopathology, Immunochemistry, Fluorescent
Image analysis of cells and tissue sections. Possess strong analytical, technical, and problem-solving
skills.
 Experienced in proteins extraction, estimation, ELISA, PAGE, Western Blots, protein expression studies,
flow cytometry, and Immunological assays.
 Knowledgeable in General Microbiology techniques including culturing, detection, drug sensitivity, TB
liquid culture, and LPA.
 Experience in working with clinical diagnostic labs, using various molecular biology and biochemistry kits,
and Immunoassays according to specifications. Also familiar with the implementation of Laboratory
guidelines quality management system ISO 15189, IQC, EQAS, and IVD medical device regulations.
 Proficient in visualizations and tables with results from statistical data analysis software.
 DRAGEN Bio-IT Platform for Illumina miseq data analysis.
 Familiar with Primer-BLAST, Primer3, Primer3Plus, SPSS, STATA, Canva, Zotero, Mendeley, endnote,
quilbot, Grammarly, Trinka, Turnitin, ithenticate, and inkspace.
 Good computer skills in Microsoft Word, Excel, and Outlook.
 Capable of designing and executing scientific experiments, analyzing and interpreting findings, and
effectively communicating results.
  Able to work in a multi-disciplinary team and possess strong organizational and project management skills.

PUBLICATIONS:

 Kommuguri UN, Satyaprasad Pallem PV, Bodiga S, Bodiga VL. Effect of dietary antioxidants on the
cytostatic effect of acrylamide during copper deficiency in Saccharomyces cerevisiae. Food Funct.
2014Apr;5(4):705-15.doi:10.1039/c3fo 60483 g. Epub 2014 Feb 14.
 Satyaprasad Pallem PV, Bodiga S, Bodiga VL. Dietary phytate lowers iron status, suppresses aberrant crypt
foci, and inhibits progression to adenoma in azoxymethane-induced colon cancer.
 Satyaprasad Pallem PV, Bodiga S, Bodiga VL. Dietary phytate reduces K-ras mutational frequency and
hydroxyl radical formation in azoxymethane-induced colon cancer.

LEADERSHIP POSITIONS:

 As a Senior Scientist at CSIR-Centre for Cellular and Molecular Biology, Satellite Centre of Andhra
Pradesh State in Vijayawada, my responsibilities include collaborating with team members, managing
procurements of kits and consumables, conducting financial audits, developing new research techniques,
providing training on NGS and Data analysis, and attending meetings.
 During my tenure as a scientist in the VRDL lab at Osmania Medical College, I conducted academic
projects for postgraduate medical students and worked closely with my team members to achieve our
research goals.
 As the Chief Scientist/Lab in charge at Osmania Medical College in Hyderabad, I supervised a team of 40
members and played a crucial role in conducting COVID-19 testing. I also developed proper guidelines for
COVID-19 testing to ensure accurate and efficient results.
 In my role as a supervisor, I led a team in conducting viral tests and provided training to lab technologists
on pre-analytical and post-analytical processes.
 I recently completed an online training program called "Discover Your Leadership Movement" offered by
the World Health Organization (WHO). I successfully completed the course and received a certificate of
completion, enhancing my leadership skills.
AWARDS & ACHIEVEMENTS

 The state medical team acknowledged and their appreciation for the skillful handling of the in-house
production of viral transport medium (VTM) to overcome the scarcity during the COVID-19 pandemic.
 Our outstanding achievement in the field of COVID-19 whole genome sequencing has been recognized by
CCMB HUB lab Hyderabad, with a remarkable 98% success rate.
 Submitted more than 2000 sequences to GISAID, IBDC, and IHIP portals.
 The Best Poster Presentation Award on Influence of Dietary Phytate Intake in Colon, Reduce Iron
Absorption and Suppresses Colon Cancer in Fisher Rats at the National Conference on Impact of
Biomarkers for Clinical and Environmental Application for Sustainable Development held on November
24th & 25th, 2017 at JNIAS Hyderabad, India.
 The Best Scientist Award was bestowed upon me in recognition of my significant role in offering COVID-
19 training and diagnostic services to Telangana State throughout the pandemic.
CERTIFICATIONS
 Hands-on training in Covid-19 Whole Genome sequencing by Oxford Nanopore Technology and Illumina
Miseq. CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
 Hands-on training Whole Genome Sequence Analysis and Bioinformatics' Pipelines by CSIR-Centre for
Cellular and Molecular Biology in Hyderabad, India.
 7-day hands-on training program on Molecular and Immunological investigations of infectious viruses.
National Institute of Virology (ICMR) in Pune, India.
 10-day hands-on training workshop on "Molecular data analysis through Bioinformatics tools" at SV
University in Tirupati, India.
 1-week training on Skill Development Program of Advanced Topics in Life Sciences on Molecular
Diagnostics by CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
 Next Generation Sequencing (NGS) for Influenza and SARS-CoV-2: From Sample Collection to Analysis
by the World Health Organization.
 Advanced Bioinformatics Data Analysis: Python, R, and Linux course organized by UDEMY.
 Served as a scientist at Gandhi Medical College, Hyderabad, India, from February 5th, 2020, to March
11th, 2020, before the outbreak of the COVID-19 pandemic in the country.
 Genetics and Next Generation Sequencing for Bioinformatics by UDEMY.
 CRISPR Cas System: Applications in Gene Editing and Beyond by UDEMY.
 Bioinformatics: Learn Bulk RNA-Seq Data Analysis from Scratch by UDEMY.
 Python for Bioinformatics: Exploring Biological Data by UDEMY.
 The Complete Data Visualization Course with Python, R, Tableau, and Excel by Infosys.
 Inequality analysis using R: Disaggregated data from surveys organized by Infosys.
 Inequality analysis using Stata: Disaggregated data from surveys organized by Infosys
 2-day hands-on training on Epidemiology, Statistics, and Data Management on Viral Diseases National
Institute of Epidemiology, Chennai, India.
 Attended 4 Days of Internal Audit and Quality Management Systems as per ISO 15189:2012 (NABL)
certification from 360 Diagnostics Delhi.
 Access Bioinformatics Databases with Biopython organized by Coursera.
 Advanced Python for data analysis course organized by Infosys
 Inequality analysis using Excel: Disaggregated data organized by Infosys.
 Introduction to R course organized by Infosys.
 Outbreak investigations in health facilities organized by the World Health Organization.
 Python Basics course organized by Infosys.
 Bioinformatics in 6 Days course offered by UDEMY.
 Primer Design For Polymerase Chain Reaction: Tips & Tricks by UDEMY
 Hands-on bioinformatics analysis from genomics to proteomics course organized by UDEMY.
 Linux Fundamentals for Bioinformaticians: A Must-Have Skill course organized by UDEMY
 Exploring Gene Expression R for Interpreting Biological Data course organized by UDEMY.
 2-week course on Next Generation Sequencing technology platforms, workflow options, and the various
tools available for data generation, analysis, and sharing by Simplilearn.
 Diagnostic network optimization (DNO) through a 2-week course provided by Simplilearn.
CONFERENCES:

 Attend National Conference on Impact of Biomarkers forClinical and Environmental Application for
Sustainable Development JNIAS Hyderabad, November, 24th & 25th, 2017 JNIAS Hyderabad, India.
 Attended International conference on Biotechnology and bioengineering-trends and got best paper
presentation JNTUH Hyderabad March 23 t o 25, 2017 JNTUH Hyderabad, India.
 Attended a National workshop on SAS and Its Application in Biotechnology and Pharmacy, organized jointly
by the Department of Biotechnology-Acharya Nagarjuna University and Configure Biopharma Services on Jan
21-23, 2010.
 Attended State Level Seminar on Future Prospects of Biotechnology organized by Department of Biotechnology,
Andhra Loyola Collage on Feb 2 &3, 2009.
 Attended a Seminar on “Food grain processing A strategic alteration to second green revolution” and presented
a poster on” Spice Oils and Oleoresins” on Sept 19-20 2008.
 Attended 2 day Seminar on Stem cells organized by NCRM in Chennai held on Oct 11, 2008.
 Attended national seminar on emergency trends in biology organized by the Center for Biotechnology and Dept.
of Zoology and Aquaculture, Acharya Nagarjuna University held on Oct 18-20 2008.

REFERENCES

Dr. Karthik Bharadwaj Dr. N Subha Lakshmi,

Scientist, Centrefor Cellular & Molecular Biology Principal Investigator, CCMB Satellite CentreAP,
Hyderabad - 500 007 Telangana, Govt. Siddhartha Medical College, Vijayawada,
India Phone: +91-99850 48590 Andhra Pradesh-520008.
Email: [email protected] Telephone: +91-78424 59716
Email: [email protected]
Dr. P Shashikala Reddy, Dr. Sudip Ghosh, Scientist D,
Professor and Principal, National Institute Of Nutrition (ICMR)
Osmania Medical College, Koti, Tarnaka Hyderabad, Telangana.
Hyderabad, Telangana500095. Telephone: +91-9849338372.
Telephone: +91-9849120002. Email: [email protected]
Email: [email protected]

Dr. B. Vijaya Lakshmi, Lakshmi Narayana R. Vemireddy, PhD

Professor, Associate Professor and
Begumpet, Osmania University, Head Department of Molecular Biology and Biotechnology,
Hyderabad, TELANGANA-500 016. Acharya NG Ranga Agricultural University,
Telephone: 91-40.23403681.
TIRUPATI-517502, AP,
Email:[email protected]
Phone: +91-94405 07965 / 6300041102
Email: [email protected]

Dr. M. Ragunath, (Rt,d). Dr. M Jafurulla, Ph.D.

Deputy Director, Scientist G, Next Generation Sequencing facility
Endocrinologyand metabolism division, CSIR-Centre for Cellular and Molecular Biology,
National Institute Of Nutrition (ICMR), Uppal Road, Hyderabad-500 007 India.
Tarnaka, Hyderabad, -500007. Phone:+91-99490 72697
Email:[email protected] Email: [email protected]