TÊN ĐỀ TÀI

SCIENTIFIC RESEARCH REPORT

Contestants: TÊN HỌC SINH 1

TÊN HỌC SINH 2
 CATEGORY

I. ABSTRACT...........................................................................................................................1
II. INTRODUCTION...............................................................................................................1
II.1. Reasons for choosing the subject.....................................................................................1
II.2. Reasearch purpose……………………………………………………...........................2
II.3. The novelty of this research.............................................................................................2
III. METHODS.........................................................................................................................2
III.1. Research materials..........................................................................................................2
III.2. Method for creating an extract.......................................................................................2
III.3. Methodfor chemical content and composition survey....................................................2
III.4. Method for testing DPPH radical scavenging activity...................................................3
III.5. Method of Monks tested the in vitro cell toxicity..........................................................5
IV. RESULTS AND DISCUSSION........................................................................................5
IV.1. Performanceextract.........................................................................................................5
IV.2. Chemical content and composition of extract…………………………......................6
IV.3. DPPH radicalscavenging activity of extract....................................................................7
IV.4. Anti-cancer activity of extract…………………………………………………………8
V. CONCLUSION……………………………………………………………………….........9
VI. FUTURE DEVELOPMENT……………………………………………………………..9
VII. ACKNOWLEDGMENT..................................................................................................10
VIII.REFERENCES.................................................................................................................10
 CATEGORY OF TABLES, PICTURES, GRAPHS

Picture IV.1. Spectrum GC/MS of DHSC extract.....................................................................6

Table IV.1. The efficiency of theDHSC extract........................................................................5

Table IV.2.Major chemical components of DHSC extract........................................................6
Table IV.3.Percentage of DPPH radical scavenging activity of DHSC extract...............................7
Table IV.4.IC50 value of DHSC extract.....................................................................................7
Table IV.5. Comparison with the IC50 values of DPPH radical trap activation DHSC extract
and the others.............................................................................................................................8
Table IV.6.Percentage ofcancer cells resistance of DHSC extract at a concentration of 100
µg/mL by Monks in vitro method..............................................................................................8
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I. ABSTRACT

II. INTRODUCTION
II.1. Reasons for choosing the subject

II.2. Research purposes

II.3. The novelty of this research

III. MATERIALS & METHODS

III.1. Research materials

III.2. Methods

III.2.1. Method for an extract

III.2.2. Method for chemical content and composition survey
Mô tả về phương pháp GC/MS
Tại sao lại lựa chọn phương pháp GC/MS? – Tức là nêu ưu điểm của phương pháp
này.

III.2.3. Method for testing DPPH radical scavenging activity

Mô tả về phương pháp bắt gốc tự do DPPH để khảo sát hoạt tính kháng oxy hoá
Tại sao lại lựa chọn phương pháp bắt gốc tự do DPPH? – Tức là nêu ưu điểm của
phương pháp này.

III.2.4. Method of Monks tested the in vitro cell toxicity

Mô tả về phương pháp thử độ độc tế bào in vitro của Monks để khảo sát hoạt tính
kháng ung thư
Tại sao lại lựa chọn phương pháp thử độ độc tế bào in vitro của Monks? – Tức là nêu
ưu điểm của phương pháp này.
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IV. RESULTS AND DISCUSSION

Kết quả phải được viết ngắn gọn và đi thẳng vào vấn đề đã nêu ra trong phần dẫn nhập.
Kết quả sẽ thể hiện qua hình ảnh, biểu đồ và bảng số liệu.
Trình bày lần lượt trả lời các mục tiêu mà tác giả đã nêu ra trong phần dẫn nhập.
 Tất cả các bảng thống kê, biểu đồ và hình ảnh phải được chú thích rõ ràng.
 Tất cả các ký hiệu, ghi tắt phải được đánh vấn hay chú giải một cách cụ thể.

Discussion
1. Giải thích những dữ kiện trong phần kết quả
2. So sánh những kết quả này với các nghiên cứu trước/khác.
3. Bàn về ý nghĩa của những kết quả
4. Chỉ ra những ưu điểm và khuyết điểm của nghiên cứu
5. Kết luận sao cho người đọc có thể lĩnh hội được một cách dễ dàng

Ví dụ:
IV.1. Performance extract
Carried out the receipt of extract and the efficiency of the sample extraction process.
The efficiency of the extract was shown in Table IV.1.
Table IV.1. The efficiency of the DHSC extract

DHSC extract

The efficiency (%) 6.58

Table IV.1 showed that the extraction efficiency recovered from methanol solvent was
6.58% higher than the average value of extraction efficiency of 4-6%, possibly because
methanol solvent was highly polarized so it diffused compounds in extract with high
polarization.

IV.2. Chemical content and composition of extract

From the result of GC/MS spectrum analysis, there are 42 constituents (See addendum
2) included in DHSC extract.
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Picture IV.1. Spectrum GC/MS of DHSC extract

Table IV.2. Major chemical components of DHSC extract

Structural
No. Name Ratio (%)
formula

1 5-Methyl-2-phenylindolizine 29.26 C 15 H 13 N

2 n-Hexadecanoic acid 5.59 C 16 H 32 O 2

3 9,12,15-Octadecatrienoic acid 4.63 C 18 H 30 O 2

4 Cyclononasiloxane, octadecamethyl- 4.01 C 18 H 54 O 9

5 Cyclodecasiloxane, eicosamethyl- 4.24 C 18 H 34 O 2Si 9

6 2,5-Dihydroxybenzoic acid, 3TMS de 4.19 C7H6O4

7 Cyclononasiloxane, octadecamethyl 4.08 C 18 H 54 O 9 Si 9

8 1,3,4-Oxadiazole, 2,5-bis 4.39 C 16 H 14 N 2O3

9 Benzo[h]quinoline, 2,4-dimethyl 3.29 C 15 H 13 N

1h-indole-2-carboxylic acid 6-
10 2.71 C 21 H 25 N O 4
ethoxyphenyl)-3-methyl-4-oxo-4,5,6

Where, 10 constituents accounting for 66.36% are presented in Table IV.2. According
to Pubchem [9], the majority of compounds are potential antioxidants, anticancers, and with
other medicinal roles.
From these, we conduct the survey of the biological activity of the exctract to asses the
ability of antioxidant, potential of hindering cancer.
IV.3. DPPHradicalscavenging activity of extract
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Conducted experiments to collect results 3 times and calculated the average value. The
result of DPPH free radical scavenging activity percentage is presented in Table IV.3.
Table IV.3.Percentage of DPPH radical scavenging activity of DHSC extract

The result of DPPH radical scavenging activity percentage (%)

Concentration (
µg/mL ¿ Standard
1st time 2nd time 3rd time Average
difference

500.0 75.68 72.65 75.52 74.62 1.71

250.0 44.79 41.29 42.72 42.93 1.76

125.0 29.40 23.61 28.03 27.02 3.03

62.5 20.10 11.48 16.28 15.95 4.32

31.25 10.17 6.45 11.75 9.46 2.72

Through Table IV.3,we found that the increasing concentration from 31.25 to 500.0
µ g /mL of the extract, the percentage of DPPH free radicals of the extract also increased from
9.46% - 74.62%. It proved that the oxidation capacity of DHSC extract is proportional to the
increase in concentration.
With the highest concentration at 500 µ g /mL the DPPH free radical scavenging
activity percentage of the extract was the highest 74.62 ± 1.71%.
Based on the above results to build a linear regression equation, to determine the IC 50
value
Table IV.4.IC50 value of DHSC extract

Concentration Q1 Q2 Q3 Qtb

T ( µg/mL ¿ 244.20 284.50 257.10 261.93 ± 20.58

Through Table IV.4, we determined the IC 50value of free radical scavenging activity
of DHSC extract is 261.93 ± 20.58 µg/mL.
Value IC50 of the research extracts and the IC50 values of the previously extracted
extracts are shown in Table IV.5.
Table IV.5. Comparison with the IC50 values of DPPH radical trap activation DHSC
extract and the others

Curcuma Boerhavia Allamanda

DHSC extract
manga [10] diffusa[11] neriifolia [12]
IC50values(µg/mL) 261.93 2.028,50 615.797 936.86
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ThroughTable IV.5, we can see the antioxidant activity by DPPH method has shown
IC50 value of DHSC extract was 261.93 µg/mL. The reason why we chose Curcuma manga,
Allamanda neriifolia, Boerhavia diffusac as three samples was because these are one of the
most successful research in Vietnam long time ago and it had variety of investments in
compounding the anti-cancer products not only domestically but also all over the world, such
as Curcuma manga that has Kukumin IP which contains curcumin phytosome with high
content is a product used in cancer treatment and to prevent cancer,... However, the IC 50 value
of DHSC extract (261.93 µg/mL) was lower than Curcuma manga (2.028,50µg/mL),
Allamanda neriifolia (936,86 µg/mL) and Boerhavia diffusa (615,797 µg/mL). The lower IC50
value of an extract is, the more optimum free radical scavenging activity of its is, make
effective in anticancer activity caused by free radicals. Thus, the obtained DHSC extract has a
more optimal capability to capture free radicals, creating an effective way in anti-cancer that
supports a lot in the making of anti-cancer products.
IV.4. Anti-cancer activity of extract
We investigated the carcinogenic activity by Monks method of testing in vitro
cytotoxicity on 3 cell lines: breast cancer cells (MCF7), lung cancer (NCI H460), liver cancer
(Hep G2) and fibroblasts (See Addendum 3).
The toxic activity of cell lines at concentration of 100 µg/mLis shown in Table IV.6.
Table IV.6. Percentage ofcancer cells resistance of DHSC extract at a concentration of
100 µg/mL by Monks in vitro method

Type of cancer Proportion that cause cell toxicity (%)

cell 1st time 2nd time 3rd time A± SD
MC F7 54.43 53.98 55.12 54.51 ± 0.57
Hep G2 73.63 73.84 74.24 73.90 ± 0.25
NCI H460 65.67 68.43 66.57 66.89 ± 1.41
Fibroblast -12.25 -13.24 -14.41 -13.30 ± 1.08
(A: Average,SD: standard difference)
(Positive values indicate toxicity,Negative values indicate non-toxic)

The resulted showed that DHSC extract had the most toxic activity in Hep G2 liver
cancer cell line with 73.90 ± 0.25%, followed by NCI H460 lung cancer cell line with 66.89 ±
1.41%. , followed by MC F7 breast cancer cell line with 54.51 ± 0.67% and non-toxic on
fibroblast line with -13.30 ± 1.08%.Extraction might cause detrimental effects on the cancer
cell, however for fibroblast, it is totally safe and the fibroblast can still develop normally.
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Therefore, while using extraction to support create product for potential toxic the cancer cell
can offer a good result more than ever.
The extract has the potential to be toxic on in vitro cell lines, showing that the extract
can also poison cancer cells in the body.

V. CONCLUSION
Kết quả có gì thì kết luận có bấy nhiêu.
The performance eviction is 6.85%.
Regarding chemical and content survey: It is determined that in the DHSC extract,
there are 42 components.
About the antioxidant ability: The value of IC50 DPPH radical trap activation of the
DHSC extract is determined to be 261.93 ± 20.58µg/mL.
Anticancer: The methanol extraction has the strongest toxicity properties when
reacting with the liver cancer cell lines HepG2 with 73.90 ± 0.25%, second is the lung cancer
cell lines NCI H460 with 66.89 ± 1.41%, third is the breast cancer cell lines MC-F7 with
54.51 ± 0.67% and is non-toxic to the fibroblasts with -13.30 ± 1.08 %.

VI. FUTURE DEVELOPMENT

Trình bày hướng nghiên cứu tiếp theo, hướng phát triển đề tài, hoặc đề tài đang tiến
hành dự kiến kết quả sẽ có trong thời gian sắp tới.
From these results, we can exploit the potential of hindering breast cancer, lung
cancer, and liver cancer from the DHSC extract.
Therefore, we will have development orientation in the future that researches to create
functional foods from combining the DHSC extract into practical applications, such as:
creating products to support cancer treatment, coming up with new antioxidants, and
contributing to biomedical and health science community all over the globe.

VII. ACKNOWLEDGMENTS
The research team would like to express our sincerity to our supervisor, M.Sc. Nguyen
Minh Trung – who wholeheartedly helped and created the most favorable conditions for us
during the project implementation period.
Our group would also like to express our gratitude to the administrators and the
teachers of Gia Dinh High School and Wellspring Saigon International Bilingual School, the
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University of Science (VNUHCM), the Department of Medicine of Ho Chi Minh City

University of Technology (HCMUT) and Ho Chi Minh City University of Pedagogy.
And last but not least, we would like to send a big thank you to those who have always
accompanied and supported us along the journey, especially to our dear families, teachers,
and friends.

VIII. REFERENCES
Chỉ tham khảo các tài liệu: các công trình nghiên cứu khoa học có DOI, từ các tạp chí
khoa học nước ngoài, các tạp chí khoa học của các trường Đại học, các trang web .org

Cách trình bày:

[1] Tên tác giả, “Tên bài báo/Tên giáo trình khoa học”, nhà xuất bản, (Năm xuất
bản).
[2] Tên tác giả, “Tên bài báo/Tên giáo trình khoa học”, nhà xuất bản, [Online] Link
bài báo, (Năm xuất bản).

Thứ tự đánh số lần lượt từ trên xuống dưới.

[1] LeThi Ngoc Ha, "Investigation of the strong antioxidant capacity of the segment of
Acanthus ilicifolius leaves IN VITRO", Can Tho University, (2015).
[2] LeThiTu Anh, “Research on chemical composition and biological activity of black-
faced jackfruit Artocarpus Nigrifolius C.Y.WU.”, Hanoi National University, University
of Natural Sciences, (2012)
[3] Pubchem, “Chemical content”, [Online] https://pubchem.ncbi.nlm.nih.gov/, (2010).
[4] Nguyen Thi Hang, Nguyen Thi Thanh Tam, Mai Huu Phuong, "DPPH's free radical
scavenging capacity and reduction power of Punernavain CanGio,Ho Chi Minh City ”,
Journal of Science Ho Chi Minh City University of Education, No. 12 (90), (2016).
[5] Tran Pham Tue Hung, Nguyen Thi My Hanh, Quach Ngo Diem Phuong,
“Study on antibacterial activity, antioxidant inhibiting tyrosinase of ethanol extracted
from Allamanda neriifolia”, University of Natural Sciences, VNU-HCM, (2014).
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Poster