chemistry pro1
chemistry pro1
chemistry pro1
SUBJECT : CHEMISTRY(043)
Certificate
Certified that this is a bonafide record of practical work done
by Mr. SYED RAMIZ in the CHEMISTRY laboratory during
the year 2024-2025
Teacher – in – charge
SL No. Topics
1 AIM
2 INTRODUCTION
3 USES OF CAFFEINE
4 EFFECTS OF CAFFEINE
5 THEORY
6 MATERIALS REQUIRED
7 PROCEDURE
8 OBSERVATION TABLE
9 RESULTS
10 PRECAUTIONS
11 CONCLUSION
12 BIBLIOGRAPHY
AIM
To determine the amount of
Caffeine present in tea
samples
INTRODUCTION
Tea is the most commonly and widely used soft
beverage in the household. It acts as a stimulant for CNS
and skeletal muscles. That is why tea removes fatigue,
tiredness, and headache. It also increases the capacity of
thinking. It is also used for lowering the body temperature.
The principal constituent of Tea, which is
responsible for all these properties, is the alkaloidcaffeine.
The amount of caffeine in tea leaves varies from sample to
sample.
Caffeine constitute about 3% of the tea’s dry
weight. Tea also contain small amount of theobromine and
theophylline, which are stimulants and xanthines similar to
caffeine
Studies have found that the caffeine contents of 1
kg black tea ranged from 22-28 mg while in green tea
ranges from 11 – 22 mg reflecting a significant difference
between the two.
USES OF CAFFEINE
1) In medicine, it is used to stimulates Central Nervous
System and to increase flow of urine.
2) Because of its stimulating effects, caffeine has been
used to relieve fatigue. But it is dangerous and one
may collapse if not consumes it under certain limit.
3) Caffeine is also used in analgesic tablets, as it is
believed to be a pain reliever. It is also beneficial in
migraines.
4) Used to restore mental alertness.
5) In certain dietary supplements used for weight loss,
and in many popular energy drinks.
EFFECTS OF
CAFFEINE
POSITIVE EFFECTS
1) Caffeine has been shown to increase the
metabolic rate and blocks the tiredness
signal receptors
2) Low dose of caffeine shows increased
alertness and decreased fatigue.
NEGATIVE EFFECTS
1) Caffeine can increase blood pressure in
non-habitual consumers.
2) Caffeine withdrawn and sleep can produce
headache, fatigue and decreased alertness.
3) High doses of Caffeine (300 mg) can cause
anxiety.
4) Caffeine can disrupt sleep and reduce blood
flow to the brain in most people.
PROCEDURE
• First of all, 50 grams of tea leaves were taken as
sample and 150 ml of water was added to it in a
beaker.
• Then the beaker was heated up to extreme boiling.
• The solution was filtered and lead acetate was
added to the filtrater, leading to the formation of a
curdy brown coloured precipitate.
• We kept on adding lead acetate till no more
precipitate has been formed.
• Again solution was filtered.
• Now the filtrate so obtained was heated until it
had become 50 ml.
• Then the solution left was allowed to cool.
• After that, 20 ml. of chloroform was added to it.
• Soon after, two layers appeared in the separating
funnel.
• The residue left behind was caffeine.
• Then we weighed it and recorded the
observations.
• Similar procedure was performed with different
OBSERVATION
TABLE
Tea samples
https://www.caffeineinformer.com/caffeinecontent/tea-
brewed
http://www.mayoclinic.org/healthylifestyle/nutrition-
and-healthy-eating/indepth/caffeine/art-
20049372?pg=2
http://www.theteaspot.com/about-tea.html