MULTIPLE CHOICE QUESTIONS

Question No. Question

1 A chromatogram is a plot of

a Detector response on Y axis against time on X axis

b. Detector response on Y axis against concentration on X axis

c. Detector response on Y axis against wavelegth on X axis

d. Detector response on Y axis against frequency on X axis

2 In Van Demeeter equation, 'A' stands for contribution to HETP due to

a Eddy diffusion
b. Longitudinal diffusion
c. Mass transfer
d. Mobile phase velocity

3 In which of the following HPLC detectors, sample is not recoverable

a Evaporative Light scattering detector

b. UV-Visible detector
c. RI detector
d. Fluorescence detector
4 An absorption spectrum is a plot of

a. Absorbance on Y axis and concentration on X axis

b. Absorbance on X axis and wavelength on Y axis

c. Concentration on Y axis and Absorbance on X axis
d. Absorbance on Y axis and wavelength on X axis
An absorption band at around 2200 cm-1 in IR spectrum would indicate
5
presence of:

a. Amide

b. Amine
c. Nitrile
d. Alcohol

A mixture of compounds A, B, C and D were separated on a Silica gel

6 TLC plate. If their polarity order is A > B > C >D,_____will have
highest Rf value

a. A

b. B
c. C
d. D
Which of the following is a sophisticated sample analysis technique for
7
determination of residual solvents
a. Headspace analysis

b. Transmission electron microscopy

c. Spectrofluorimetric analysis
d. Dissolution studies
_________ type of anions will be held more strongly on an ion exchange
8
column
a. Fluoride ion

b. Chloride ion

c. Iodide ion
d. Sulphate ion
9 Quantum efficiency is a term related to
a. Absorption spectroscopy
b. X- ray diffraction
c. Fluorescence spectroscopy
d. Thermal methods
10 Atomic absorption Spectra is characterized by:
a. Band spectra
b. Combination of line and band spectra
c. Either line or band spectra
d. Line spectra
 In separation by paper chromatography, Rf value of Q will be _____, if
11 P travels 3 cm and Q travels 6 cm from application line and the solvent
front has travelled 9 cm
a. 0.3333
b. 1
c. 0.6666
d. 0.9999
Which of the following is the disadvantage of Hydrogen gas being used
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as a carrier gas in gas chromatography
a. Combustible nature
b. Expensive
c. Reduced sensitivity
d. High density
13 Gradient elution in HPLC involves_____
a Changing the mobile phase composition with time
b. Successive injection of the sample
c. Changing the length of the column
d. Gradual decrease in the number of theoretical plates
14 Conjugation in a chromophore results in:
a. Shifting of absorption band to shorter wavelengths
b. No change in position of the absorption band
c. Disappearance of absorption band
d. Shifting of absorption band to longer wavelengths

For any compound to get analyzed by IR spectroscopy, the compound

15
should have:_______
a. Existence of dipole moment
b. Double bond
c. Triple bond
d. Benzene Ring
A mixture of compounds A, B and C was separated on 12 cm silica gel
TLC plate. Solvent front was allowed to run up to 9 cm. If A, B and C
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travelled distances of 2cm, 7cm and 5cm, the increasing order of
polarity of A, B and C will be
a. A>B>C
b. A<B<C
c. A>B>C
d. A=B=C
Main characteristic of an isocratic method as compared to gradient
17
elution method is
a. composition of mobile phase remains constant
b. pH of mobile phase is changed

c. proportion of aqueous solvent in mobile phase is increased

d. proportion of organic solvent in mobile phase is increased

18 Resins of ion exchange are formed by polymerization of styrene and

a. Benzene

b. Chlorobenzene

c. Divinylbenzene
d. Toluene
19 UV - Visible radiation can bring about which transitions in a molecule

a. Electronic + Vibrational + Rotational transition

b. Only vibrational transition

c. Only rotational transitions

d. Only vibrational + rotational transitions

20 The following are types of bending vibrations except _____________

a. Scissoring
b. Asymmetric stretch
c. Twisting
d. Wagging
____________ detector used in HPLC allows detection at different
21
wavelengths simultaneously
a. Fluorescence
b. Electron capture

c. Flame ionization
d. Photodiode array
22 Fluorescence involves conversion from

a. Triplet excited state to ground state

b. Singlet excited state to ground state

c. Ground state to triplet excited state
d. Ground state to singlet excited state
 A solution of sodium chloride is to be analyzed for its Sodium content.
23
The most suitable method for the same would be
a. UV spectrophotometry
b. IR spectroscopy
c. Fluorescence spectroscopy
d. Flame photometry
24 Chromatography is a ___ technique
a. Separation
b. Extraction
c. Thermal Analytical
d. X-ray diffraction
Which of the following situation in isocractic elution warrant use of
25
gradient elution in HPLC
Large difference in the retention times of first and last eluted
a.
components
b. Tailing of peaks
c. Unstable baseline
d. Variable retention time
26 Stationary phase used in Gel chromatography
a. Polyacrylamide gel
b. Silica gel
c. Alumina
d. Cation exchange resin
27 Which of the following is used as a light source in UV spectroscopy?
a. Xenon arc lamp
b. Deuterium discharge lamp
c. Incandescent lamp
d. Mercury vapor lamp
In atomic absorption spectroscopy, the most strongly absorbed light is
28
called as
a. Resonance line
b. Base line
c. Stokes line
d. Anti-stokes line
29 In electrophoresis,if the voltage of the electric field is increased,
a. rate of migration of charged particle decreases
b. rate of migration of the charge particle increases
c. No change in the migration rate of the charge particle
d. Particle becomes immobile
 In HPLC, an isocratic elution is the one in which the composition of
30
mobile phase:
a. Changes with time
b. Remains constant with time
c. Stepwise increment from polar to nonpolar
d. Stepwise increment from nonpolar to polar
The chromatographic method of separating biochemical mixtures of
31 compounds based on highly specific biological interactions is referred to
as _______________
a. Thin layer chromatography
b. Ion-exchange chromatography
c. Gel permeation chromatography
d. Affinity chromatography
32 The purpose of secondary filter in fluorescence spectroscopy is:
a. Allows only excitation radiation
b. Allows only emission radiation
c. Allows both emission and excitation radiation
d. Allows only transmitted radiation
33 Mid IR region consists of?
a. 1400 - 600 cm-1
b. 2000 - 1000 cm-1
c. 4000 - 400 cm-1
d. 2500 - 500 cm-1
34 _____________ is a bulk property detector used in HPLC analysis
a. Electrochemical
b. Evaporating light scattering
c. Fluorescence
d. Absorbance
35 The wavelength range of visible spectroscopy is from?
a. 10 - 200 nm
b. 200 - 400 nm
c. 800 - 1000 nm
d. 400 - 800 nm
In Atomic Absorption Spectroscopy, which of the following is the
36
generally used radiation source?
a. Tungsten lamp
b. Xenon arc lamp
c. Hollow cathode lamp
d. Hydrogen lamp
37 In Gas chromatography, derivatization of a sample is carried out to:
a. increase polarity of the analytes
b. increase volatility of the analytes
c. decrease solubility
d. Decrease detector response
38 The best method to differentiate proteins is by _________________
a. Paper chromatography
b. Ion-exchange chromatography
c. Gel chromatography
d. Gas chromatography
39 How many filters are used in Fluorimeter?
a. One
b. Two
c. Three
d. Four
40 Flame photometry is used for the quantification of?
a. Salts
b. Metal ions
c. Organic compounds
d. Residual solvents
41 In electrophoresis, as the ionic strength of the buffer is decreased,
a. rate of migration of charged particle decreases
b. rate of migration of the charge particle increases
c. No change in the migration rate of the charge particle
d. Particle becomes immobile
42 Van Demeeter equation gives an estimate of
a HETP
b. Peak tailing
c. Peak area
d. Resolution
43 Asymmetry factor is calculated at ____ % of peak height
a 5
b. 10
c. 15
d. 20
_____ is the ability of the chromagraphic system to chemically
44
distinguish between sample components
a Selectivity factor
b. Tailing factor
c. Capacity factor
d. Asymmetry factor
45 Which among the following is an example of bulk property detector.
a Refractive Index Detector
b. UV-Visible Detector
c. Fluorescence Detector
d. Electrochemical detector
46 Gradient elution in HPLC involves_____
a Changing the mobile phase composition with time
b. Successive injection of the sample
c. Changing the length of the column
d. Gradual decrease in the number of theoretical plates
47 Headspace analysis techniques is used for______
a sampling and examining residual solvents
b. examining Highly polar samples
c. examining Highly non-volatile samples

d. examining deuteriated samples

In which of the following type of paper, chromatography does the mobile
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phase move horizontally over a circular sheet of paper?
a Radial
b. Ascending
c. Descending
d. Ascending-Descending
49 Sephadex is the name of the stationary phase used in?
a Gel chromatography
b. Ion pair chromatography
c. Ion exchange chromatography
d. Planar chromatography
50 Conjugation in a chromophore results in:
a. Shifting of absorption band to shorter wavelengths
b. No change in position of the absorption band
c. Disappearance of absorption band
d. Shifting of absorption band to longer wavelengths
For any compound to get analyzed by IR spectroscopy, the compound
51
should have:_______
a. Existence of dipole moment
b. Double bond
c. Triple bond
d. Benzene Ring
52 In a Fluorimeter, the filter placed after the sample cell is called as
a. Secondary filter
b. Primary Filter
c. Interference Filter
d. Excitation filter
53 _______ is used as a source in Atomic absorption spectroscopy
a. UV lamp
b. Mercury Arc lamp
c. Hollow cathode lamp
d. Flame
54 Rf value in thin layer chromatography
a. Less than zero
b. Between 1 to 10
c. Always more than 1000
d. Always less than 1 but more than 0
Name the terminology which involves increasing the temperature of a
55
gas chromatograph column as a function of time
a. Isothermal run
b. Temperature programming
c. Isocratic elution
d. Gradient elution
Best technique which can be used for separation of different sizes of
56
peptides from a mixture can be
a. Thin layer chromatography
b. Ion-exchange chromatography
c. Gel filtration chromatography
d. Affinity chromatography
57 A UV spectrum is a plot of?
a. Peak intensity versus wavenumber
b. Absorbance versus wavelength
c. Absorbance versus wavenumber
d. Peak intensity versus wavelength
In nephelo turbidimetry, the intensity of scattered light is measured at
58
____ angle
a. 60°
b. 180°
c. 90°
d. 30°
Name the technique in electrophoresis in which the stationary phase
59
employed is in liquid state
a. Polyacrylamide Gel electrophoresis
b. Paper electrophoresis
c. Capillary electrophoresis
d. Cellulose acetate Gel electrophoresis
60 Which detector is used in Gas chromatography?
a. Electron capture detector
b. Bolometer
c. Photomultiplier
d. Pyroelectric detector
61 Recognizers used in Affinity chromatography are:
a. Specific type of molecules bound to stationary phase
b. Specific type of molecules to be included in the mobile phase
c. Multiple utility type of molecules bound to stationary phase
d. Multiple utility type of molecules to be included in the mobile phase
Which one of the following is an example of a monochromator used in
62
UV-vis spectroscopy?
a. Interferometer
b. Filter
c. Prism
d. Light source
63 ____colour flame is shown by Na ions Flame photometry?
a. Blue
b. Green
c. Yellow
d. Red
64 Ultrasonication of HPLC mobile phase is performed to
a. Increase its resolving power
b. Increase its temperature
c. Remove dissolved gases
d. Remove entrapped ions
65 Which of the following is used as light source in fluorimetry?
a. Mercury vapor lamp
b. Incandescent wire
c. Xenon arc lamp
d. Deuterium discharge lamp
66 Atomic absorption spectroscopy is used for the detection of?
a. Organic compounds
b. Metals
c. Residual solvents
d. Non-metals
What is the main charateristic of an isocratic elution to a gradient elution
67
method

a The mobile phase composition remains constant

b. The proportion of aqueous solvents in mobile phase is increased

c. The proportion of organic solvents in mobile phase is increased

d. The pH of the mobile phase changes continously

68 In Van Demeeter equation, 'C' stands for contribution to HETP due to?

a Mass transfer
b. Longitudinal diffusion
c. Eddy diffusion
d. Mobile phase velocity
Which of the variables associated with a chromatogram is used in
69
quantitative analysis

a Peak area

b. Retention time
c. Tailing factor
d. Adjusted retention time
70 The guard column is positioned_______.
a Just before the main analytical column
b. Just after the main analytical column
c. Before the sample injection port
d. Just before the detector
71 What is true about RP-HPLC
a Non-polar stationary phase and Polar mobile Phase

b. Polar stationary phase and Non-polar mobile Phase

c. Polar stationary phase and polar mobile phase
d. Non-polar stationary phase and non-polar mobile phase
Separation of mixtures in GC at same temperature conditions throughout
72
the elution is referred as__________?

a Isothermal elution
b. Gradient elution
c. Temperature programmed elution
d. Isocratic elution
Name the term to denote the amount of time an analyte spends in the
73
column after it has been injected
a Retention time
b. Capacity factor
c. HETP
d. NTP
74 Chromatography is a ___ technique
a Separation
b. Extraction
c. Thermal Analytical
d. X-ray diffraction
75 Helium purging in HPLC is done for_______
a removal of dissolved gases from mobile phase
b. increasing the pressure of the mobile phase
c. increasing the rentention time in chromatogram
d. removal of particulate matter from mobile phase
Which HPLC detector is best suited for peak purity analysis & impurity
76
profiling
a PDA detector
b. Fluorescence detector
c. RI detector
d. ELS detector
Which among the following gases is usually recommended to be used as
77
a carrier gas in GC?
a Nitrogen
b. Oxygen
c. Carbon dioxide
d. Sulphur dioxide
78 Rf value in TLC is?
a Between 0 - 1
b. Between 10-100
c. Between 100-1000
d. More than 1000