Desi

gnandLay
outofAni
malTi
ssueCul
tur
eLabor
ator
y

Cul
tur
eLabDesi
gn

Theneedt omaint
ainasepsisdi
sti
ngui
shest
heti
ssuecult
urelaborat
oryf
rom mostot
her
s,soi
t
i
simportanti
tbedustfree.
Thei
ntroduct
ionoflami
narflowhoodshasgr
eat
lysi
mplif
iedtheproblem.

Layoutdependsont hetypeandscal
eoft heoperat
ionsandt henumberofuser
s,butsome
generalpri
ncipl
esapply.
Animaltissuecult
urelabdesi
gnforeasycl
eani
ng.Furnitur
eshouldfi
tti
ght
lyt
othefl
oororbe
suspendedf r
om thebenchwit
haspacelef
tunderneat
hf orcl
eani
ng.

Coverthefloorwi
thacov edv i
nyl
,acr
y l
i
ccoating,
orotherdustpr
ooffi
nish,andall
owaslightfal
l
i
nt heleveltowar
daf loordr ai
nlocatedoutsi
dethedooroft heroom.Thisarrangementall
ows
l
iberaluseofwaterifthefloorhastobewashed, butmoreimportant
,itpr
otectsequi
pmentfrom
damagi ngfl
oodsifsti
l
ls,autoclav
es,orsinksover
fl
ow.

Ani
mal
TissueCul
tur
eLabor
ator
y

Requi
rement
s

Mat
eri
als
Pr
ovi
sionmustbemadef
orpr
epar
ati
onandst
eri
l
izat
ion,
asept
ichandl
i
ng,
otheract
ivi
ti
eswi
thi
n

t
hecul
tur
ear
eai
ncl
udi
ngcent
ri
fugat
ion,cel
lcount
ing,mi
croscopy
,incubat
ion,andst
orageat

r
oom t
emper
atur
e,4⁰
C,−20⁰
C,and−196⁰
C.
Numberofusersdeterminehowmanylaminar
-f
lowhoodswill
berequi
red)
andwhetheral ar
gear eawi l
lbeneededtohandl
ebioreact
ors,ani
maltissuedi
ssect
ions,or
l
argenumbersofcultur
es.

Space

Thelargestar eashouldbegi ventothecul tureoperati

on,whi chhastoaccommodat elaminar-
fl
ow hoods,cel lcounters,centri
fuges,incubators,micr
oscopes,andusi ngstocksofreagents,
media,glasswar e,andpl ast
ics,and,ifpossi bl
e,aquar antineareainanimalti
ssuecult
urelab
desi
gn.
Thesecondl argestisforwashup, preparat
ion,andsteril
izati
on,thet
hir
disforstor
age,andthe
four
thisforincubat i
on.
Pr
epar
ati
onAr
ea

Facil
iti
esforwashi ngupandf orsteri
li
zat
ionshouldbelocat
ed
(a)closet otheasepticareathattheyser
v i
ceand
(b)onanout sidewalltoall
owf orthepossibi
l
ityofheatext
ract
ionf
rom ov
ensandst
eam v
ent
s
from autoclavesinanimalti
ssueculturel
abdesign.

St
orage
St
oragemustbepr
ovi
dedf
ort
hef
oll
owi
ngi
tems
(
1)St
eri
l
eli
qui
ds,
atr
oom t
emper
atur
e(sal
tsol
uti
ons,
wat
er,
etc.
),at4◦
C(medi
a),
andat−20◦
Cor

−70◦
C(ser
um,
tr
ypsi
n,et
c.)
(2)Steri
leandnonst eri
leglassware
(3)Steri
ledisposableplasti
cs(e.g.,cult
uref
lasksandPet
ri
dishes,
cent
ri
fuget
ubesandv
ial
s,and
syri
nges)
(4)Screwcaps, stoppers,etc.
(5)Apparatussuchasf i
lt
ers,steri
leandnonsteri
le
(6)Gloves,disposalbags,etc.
(7)Li
quidnitrogentor epl
enishf r
eez er
s

Access
Makesur ethatdoorwaysarewi deenoughandhighenoughandt hatcei
li
ngshavesuffi
cient
cl
earancetoallow t
hei nst
all
ati
onofequipmentsuchasl aminar
-f
low hoods,i
ncubat
ors,and
aut
oclaves.Pr
ovidespaceforaccessf
ormaint
enanceofequipment.

Steri
li
ty
Thisisoneofthemorediff
icul
tprobl
emsbecausecont ai
nmentrequi
rest
hatthecontent
soft
he
ti
ssueculturer
oom notescapetoadjacentworkareas,whil
easepsi
srequi
resthatnoneoft
he
contaminati
onofsur
roundi
ngareasentersthet
issuecult
ure.

I
ti sessent i
altohav eadedicatedti
ssueanimaltissuecul
tur
el abdesi gnwithanadj acent
preparati
onar ea,oranumberofsmalleroneswithacommonpr eparat
ionarea,rat
herthant o
havet i
ssuecul tur
eperf
ormedalongsi
deregul
arlaborator
yworkwi t
honl yalaminar-
fl
ow hood
forprotect
ion.

Aseparatefaci
li
tygi
vesbettercontaminati
onprotect
ion,al
l
owsti
ssuecul
tur
est ockst
obekept
separat
efrom regul
arl
aboratoryreagentsandglassware,andwi
ll
,inanycase,berequi
redf
or
contai
nmentifhumanorotherprimatecellsar
ehandled.