Experiment - 3

Download as docx, pdf, or txt
Download as docx, pdf, or txt
You are on page 1of 3

EXPERIMENT:-03

AIM:- To construct a calibration curve of Paracetamol (API) using U.V Spectroscopy.

REFERENCE: Behera S, Ghanty S, Ahmad F, Santra S, Banerjee S (2012) UV-Visible


Spectrophotometric Method Development and Validation of Assay of Paracetamol Tablet
Formulation.

REQUIREMENTS: Apparatus Required: Volumetric flask, Pipette, measuring cylinder,


Pipette Chemical: Paracetamol (API) Instrument: UV visible spectrophotometer.

THEORY:
Paracetamol is a common over-the-counter medication used to relieve pain and reduce fever.
Here's how to interpret the information on a paracetamol tablet:
Strength: The strength of the paracetamol tablet will be indicated, typically in milligrams
(mg). Common strengths include 500 mg or 650 mg. This tells you how much paracetamol is
in each tablet.
Dosage Instructions: The packaging will provide instructions on how to take the tablets,
including the recommended dosage, frequency, and maximum daily dosage. Always follow
these guidelines.
Indications: The packaging should specify what the medication is used for, which is
typically for pain relief and reducing fever.
Uses:
This medication is used for the temporary relief of runny/stuffy nose, watery/itchy eyes, and
itchy throat caused by allergies, hay fever, the common cold, and other breathing illnesses.
This product is usually not used ongoing cough from smoking or long-term breathing
problems (such as chronic bronchitis, emphysema) unless directed by your doctor. The
decongestant treats nasal congestion by narrowing the blood vessels in the nose. The
antihistamine relieves itchy/watery eyes and itchy throat by blocking a substance (histamine)
released by allergies. The anticholinergic dries up a runny nose and the fluid that runs down
your throat causing irritation.

PROCEDURE.

Preparation of 0.1 N NaOH


W=NEV/1000
W=0.1*40*100/1000
W=0.4 gm in 100 ml distilled water.
Preparation of standard stock solution of paracetamol.
1. Weigh 10mg paracetamol and transfer to 100ml volumetric flask containing 20ml
of 0.1N NaOH.
2. Makeup the volume to 100 ml with distilled water.
Preparation of sample solution.
1. Take 10 ml of standard solution and transfer into 100 ml volumetric flask and make up the
volume up to 100ml. (10 µg/ml concentration of sample).
2. Make different concentration of sample solution (2, 4, 6, 8 ,10 and 12 µg/ml) primary
sample solution in 10 ml volumetric flask.

 Measure absorbance of different secondary sample solution correspond to concentration


plot a graph.

Calculation: -
 Concentration of standard solution.
 10mg-------100ml
 1mg--------10ml
 1000µg-----10ml
 100 µg-----1ml
 Calculation of primary sample solution.
 10 ml of standard soln in 100 ml distilled water.
Conn = 100 * 10 / 100 = 10 µg/ml.
OBSERVATION: -

Calibration Curve for Paracetamol


1.4

1.2

1 f(x) = 0.0803742857142857 x + 0.157446666666667


Absorbance

0.8

0.6

0.4

0.2

0
0 2 4 6 8 10 12 14
Concentration µg/ml
Concentration Absorbance
µg/ml.
2 0.3498
4 0.5312
6 0.572
8 0.7442
10 0.9101
12 1.2133
y = mx + c, y=absorbance, x=concentration,

c= intercept = 0.1574, m=slope = 0.0804

RESULT: A calibration curve of Paracetamol (API) using U.V Spectroscopy is done.

You might also like