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    Centrifuge

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    Abi S

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    © All Rights Reserved
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    Centrifuge

    Uploaded by

    Abi S
    5 views12 pages

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    © © All Rights Reserved

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    Download as docx, pdf, or txt
    5 views12 pages

    Centrifuge

    Uploaded by

    Abi S

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 12

    CENTRIFUGE

    Discovered by ANTONIN
    PRANDTL , in 1864.

    INTRODUCTION :
    CENTRIFUGE is a mechanical
    process that utilizes a spining
    medium to separate one or more
    components of a sample according
    to density or size.
    CENTRIFUGEL separations may be
    classifled as either analytical or
    preparactive.
    E.g: molecular weight, shape, and
    association.

    TYPES OF CENTRIFUGE:

    Benchtop centrifuge Continuous


    flow centrifuge Gas centrifuge
    Hematocrit centrifuge
    High-speed centrifuge Low-
    speed centrifuge
    Microcentrifuge
    Refrigerated centrifuge
    Ultracentrifuge
    CENTRIFUGE DEFINATION:

    The centrifuge is commonly used


    in laboratories for the separation of
    biological molecules from a crude
    extract.

    In a centrifuge ,the sample is kept


    in a rotor that is rotated about a flxed
    point (axis) ,resulting in strong force
    perpendicular to the axis.
    There are different types of
    centrifuge used for the separation of
    different molecules ,but they all work
    on the principle of sedimentation.

    BENCHTOP CENTRIFUGE:

    Also known as multipurpose


    centrifuge .

    USES:

    pellet cells and bacteria.


    flxed angle
    rotor:17,000g/14,000rpm.
    swinging bucket
    rotor:3,800g/4,800rpm.

    CONTINUOUS FLOW CENTRIFUGE:

    Used industrially and for few lab


    separations like recovery of bacteria
    from litres of culture solution.

    The sample is ejected through the


    centre and separated at a
    speciflc speed.

    GAS CENTRIFUGE:

    Uses physical principle of centri


    petal force to separate U-235 from
    U-238.

    very high speed rotor generates


    centripetal force.

    separation increases with rotor


    speed and length.
    HEMATOCRIT CENTRIFUGE:

    Micro : Hematocrit centrifuge


    dsc-100MH-1.

    Speed : 12,000rpm(flxed).

    Motor : universal motor.

    Timer : 0-15min . Mechanical


    timer.

    Weight (kg) : 4.5/ 5.0kgs.


    HIGH-SPEED CENTRIFUGE :

    15,000-20,000Rpm.

    Lorge sample capacity depending


    on rotor.

    normally refrigerated.

    research applications.

    differentiation separation of
    nucleus , mitochondrial , Protein
    precipitate , large intact organclle
    , cellular debris , bulky protein
    aggregates.

    LOW -SPPED CENTRIFUGE:

    The low speed centrifuge has a


    maximum speed of 4000-
    5000rpm.

    These instruments usually operate


    at room temperature with no means
    of temperature control.

    Two types of rotor are used in it


    flxed angle and swinging bucket.
    MICRO CENTRIFUGE:

    Microcentrifuges are used to


    process small valumes of biological
    molecules cells, or nuclei.

    Microcentrifuges are small enough


    to flt on a table -top and have rotors
    that can quickly change speeds.

    Maximum angular speeds of


    12,000-13,000rpm.
    REFRIGERATED CENTRIFUGE:

    Refrigerated rotor champer.

    capacity to change rotor


    chambers for varying size

    Spin speed upto 6500g and


    sediment rapidly like erythrocytes
    , yeast cell , nuclei and
    chloroplast.

    ULTRACENTRIFUGE:
    It is an important tool in
    biochemical research.

    It is high speed vacuum- type


    centrifuge.

    It discovered by swedberg in 1920.

    speed of more than 60,000rpm.

    Temperature is not raised..

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