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    CP 09 - Factors Affecting The Rate of Aerobic Respiration

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    CP 09 - Factors Affecting The Rate of Aerobic Respiration

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    damizory
    8 views3 pages

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    CP 09 - Factors affecting the rate of aerobic respiration

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    8 views3 pages

    CP 09 - Factors Affecting The Rate of Aerobic Respiration

    Uploaded by

    damizory

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Edexcel B Biology A-Level

    Core Practical 9
    Investigate factors affecting the rate of aerobic respiration using a
    respirometer

    www.pmt.education
    A respirometer is a piece of equipment which measures ​rate of respiration​. It works by
    the addition of a​ drop of coloured liquid ​to a length of tubing. As the organism respires
    and the volume of oxygen in the tube decreases, the ​pressure also decreases​ and the
    liquid moves down the pressure gradient towards the respirometer.

    Equipment
    ● Respirometer
    ● Live animals (e.g. maggots, woodlice)
    ● Respiring and germinating seeds (e.g. peas)
    ● Soda lime wrapped in muslin
    ● Manometer fluid
    ● Spatula
    ● Stop clock
    ● Clamp and stand
    ● Pipette
    ● Balance

    Method
    1. Assemble the respirometer.
    2. Add 5g of one organism to the boiling tube and replace the bung.
    3. Place a drop of c​ oloured manometer fluid​ in the open end of the respirometer. Use a
    syringe​ to draw the fluid as far from the respirometer as possible and record its
    starting position.
    4. Close the tap​. Start the stop clock.
    5. After five minutes, open the tap. ​Record the end position​ of the coloured liquid.
    6. Repeat the process for the other organism.

    www.pmt.education
    Risk Assessment

    Hazard Risk Safety In emergency Risk Level


    Precaution
    Broken glass Cuts from Take care when Elevate cuts; Low
    sharp object handling glass apply pressure;
    objects; keep do not remove
    away from edge glass from
    of desk wound; seek
    medical
    assistance
    Soda lime Corrosive Wear eye Wash off skin Low
    protection; avoid immediately;
    contact with skin flood eye/cuts
    with cold water
    Biohazard Contamination Use disinfectant; Seek assistance Low
    wash hands with
    soap after
    handling
    organisms

    Analysis
    ● Convert distance moved by the liquid in the time into volume of gas by using the ​πD
    formula with the diameter of the respirometers tube to produce a ​cross-section
    and then multiplying by distance moved.
    ● Convert volume into ​rate​ by dividing by five minutes.
    ● Convert rate into rate per gram of organism by dividing by five grams.

    Conclusion
    ● Soda lime absorbs carbon dioxide​ given out during respiration so any changes in
    volume are assumed to be only due to differences in oxygen uptake.
    ● Gas exchange due to ​photosynthesis​ is ignored and all of the gas is assumed to
    be oxygen.
    ● Different organisms have different rates of respiration - the ​animals have a higher
    rate of respiration​ per gram than the plants, as they have a ​higher metabolic rate
    and require much more energy to be released for movement/reproduction/etc.

    www.pmt.education

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