ifeng.

ae 48h88 ÉÉm
yay
t
won
110dalton an mac I formdoesn't
21 are tospanamembrane

tram.tn oft I

Chapter 11

Small-Molecule Transport
and Electrical Properties of
Membranes
Signaling Modality
a to
acts as layer
modulate selective
of all Lecture 5
travelling ingot
0021021720Stfidones Ed
FIalys
Copyright © 2022 W. W. Norton & Company, Inc.
IIe Arcee can carry out Not Protein exit ER lumen in
vessiles intra
Phagocytosis icy proteins ofplasmamedia
out
go
in version
Neuronal cells replicate twigging
glycoproteinsproteoglycans

Not protein endocytosed

whentime's up
PFTgh membrane Masaki

I. Introduction
II. Permeability properties of Lipid
Bilayer (Synthetic)
 Smart
more hydrophobic

more inn

lager
becomes
harder
 103

Note
for every trilling's
one Nat ion can
cross the
membrane
III. Transport of small molecules
across membrane:
 Ifm

Carter

Oligomerproteins
coming together
Confmational upon
Change
binding
g g

allow passage of
soliton from
lout
higher
CARRIER PROTEINS
transporter proteins
always passin both

to low
high
driver
by affinity
 Carrier

opposite
One Samaon direction
no leak
in one
direction
Natist pump
AntiportMetre Conformationalenergy
coupledtransport
drum
by affinity

pockets for
Nat pockets
Kt
f

4th
Edition
 Apical
bone
foundon

ERmembrane
JDIsonneer
CHANNEL PROTEINS
V4 diff
mechanisms
open dose
 binds

Chanel
open

Complex
of
proteinchargingGfomaton

gated
Voltage
tittigrts
setearly anycharges phospholipids have
todowith
Openingand
dog
eurotrannies
released
bindingto
receptor
10
of
 Musclecontractor is
acres a modelof howcytoskeleton
Actomyosin can remodel chef

obits e
Hall venulesto

scored
Horae
cellsaorta
impulse
to headstowards
cartonsinto
917 kinase pretty
bindtactrate Phosphorylates
Protein king motor protaneggotghtorytonation
 yes

Proteins
IV. Transport of large molecules
across membrane allow
transport
Exo Endo
in both
dreams
Transport of Large Water Soluble
Molecules Across the Membrane

Exocytosis
fusion to
empty organ Complexprocess
both basal and preside
it contain Tysteergne
 Edging
stg f
Ptagnateby E
exocytosis
once in the ER
MRNAtranslated in a
largehallway

Ig g of protons
Sequences

Ksan avg.ggEE
fitted.EE
4giititD in fusion with Release FEE
fE.by
anon

fetid o
anally
bag
depending onprotein toevents
pigsty happening
Gulated orcontinueSection kin
thisdecision present in
mug
TEM image Ale is 10mm
so this is 115thofall
 T

ez

y
milkprotein

tocytosol
get
and
otherversicles
Figure 13-62 Molecular Biology of the Cell (© Garland Science 2015)
fusewith them
 every vesicle moving from on complex to another it will
have a multitudeofprotons Motorprotein as it
Ey afenthe II cytoskeleton
has to be propelled on cytoskeleton
Coatproteins ftp.rinveniuefomatondnng
coatmer protein
endocytosis single
seatEffigy'Entia
gig amie
pm Feiffer
Figure 13-4 Molecular Biology of the Cell (© Garland Science 2015)
finite all that a protein to
pathways in
cog

fegggg.tt
antagramoto fomERtogolgi

crotur path
So
any
venial pay off has
outproth help in
ornery
andachy
Emerge
up
Transport of Large Water Soluble
Molecules Across the Membrane

wmonent of transdeeminia
Integral th are signal

Endocytosis
encompasses
pinocytosis end
phage
layetibificent
tamanapffpngolend
Feig ay
uptake of protein
complex ax
ligand
membrane
Sona
and takin are
liquids but
goin
beesdon'tanklet
aqupon
thyhythfag
É
baa
Endogh vesicle Process ofhoncho at girl

membrane
ligand

bind
to
Eat
 crumbles into Triskelion

Endgtz Venite Gatlin wand

end
uefming
lysosomes

e5
except
É
it speak
 to
Triskelion softy
ad onTriola
charm
Blightseevid on

this

Figure 13-6a, b Molecular Biology of the Cell (© Garland Science 2015)

Tiskehon hexagonal pentagonal network hefty in process of Venner fond

Figure 13-6c, d Molecular Biology of the Cell (© Garland Science 2015)

 E
In

proof 11 ELITE
associate

LDL bind to bus receptor lay to contomenon

Change as IF dittsion isundergone
to it
clathrin bindsto adopt protein span
clathrin Triskelion stirs assembly
hey I
preen f
comes

fogies
 endogn
Vonizle O
tastily
tie
tar
pit
11175
is
to
earlyends
doing go
to iH
angst

arenas un Iya
when is aged exert it
a
war
Figure 13-56 Molecular Biology of the Cell (© Garland Science 2008)
fonts retard on tu
 LDL/Cholesterol Receptor

Eddie
j
su

É
faena into a
diff direction

mateltson trynna

0
Alena into
degrees LDL
adtranenato
Figure 13-52 Molecular Biology of the Cell (© Garland Science 2015)
 enetically

Eisposia
unable to

endocytose

People susptible
mfr
Atherosclerosis
high conc of LDL-cholesterol in blood
han a mutation so
taptin Git
b'add
in
acumen
member
atherosclerosis
fry
Figure 13-51 Molecular Biology of the Cell (© Garland Science 2008)
Cholesterol retrieval (export) by reconstituted
from fragments of Niemann-Pick C1 (NPC1)
(findings by Brown, Goldstein, et al. [UTSW])
Choon

1300dg about size Yukeight

ofsthoid
Cholesterol Retrieval Process
Cholesterol to pockets 1 protons confirmation al
binding undergoing change
 Niemann-Pick Type C disease

• Fatal lysosomal lipid storage disease caused by NPC1 &

NPC2 gene mutations

• Entails wide and varied clinical spectrum (splenomegaly,

hepatomegaly, hepatosplenomegaly, progressive neurological
disease which naturally leads to death/ disability, etc.)

Mutation a dithers mutation can be

causing Vand to
leading
a wide Spectrum
of
dishes
Endocytic Vesicles

LDL
EGF
Transferrin
IgG
(transcytosis)

Figure 13-52 Molecular Biology of the Cell (© Garland Science 2008)

 transact
endocytosis singepartofsyne padding both receptor nagana degraded

Fix
alwaysacts
downgrade
han
doesn't

HIIIII body
EGF Receptor
fuel epithelial cell
Senk EGF
as bind to
Eff theyreleptor

0.0 É t recestrygdoesn't
necessary men

Ironphosphorylates
set
of
phosphorylation

ofEERO offer
to Eff
minty
signypay
IET
continue

Upregulation

To
glossatordyes denof
rhyme as Reept down
gud
 Transferrin Receptor pÉ

uomo
oeaa me
in milk
lactfant another iron binding proton

Jenn non
any man an

Thou both ligand and rept arity

and

evading lysosome

Afgan
transfer abodes before big iron

upon binding confmenu Chaya as it

has to TFR
affinity toggay exocytosis
Tra
nsfe
rrin
(TF)
Cycl
e
for
Euk
aryo
tic
iron
upta
ke
bring
in
enough
in I fort a a
dow TAR
gun
Tra
nsfe
rrin
(TF)
&
Ferr
itin
mR
NA
regu
latio
n by
IRE
-BP
 wehavelocalimmunity human
IGA amendedour imma gin tagton
IgG/IgA Receptors
men committing
mother's j g
IgG
izmasibrateatninewn'ÉÉIÉÉÉ
REI.EE
• Transcytosis
infinite
war

receptor And its for basal

and rehear at gas Arial
Ice water and process
or the isotone endocyth
 IgG retrieved from milk in the small
intestine.
The pH of the small intestine is around 6.0,
and the pH and the basolateral side is 7.5. This
change in pH is enough to release the IgG from
the Fc receptor which remains intact.

Polymeric immunoglobulins (IgA and IgM),

and the polymeric Ig receptor (pIgR).

For the secretion of IgA in the mucus layer

lining epithelial cells. The IgA-pIgR complex
is enzymatically cleaved either in the
transcytotic vesicle or at the apical side in
order to release IgA. In fact, an amino acid
sequence originating from the pIgR will still be
ma ing bound to IgA, and it is called the secretory
component. The secretory component protects
IgA from enzymatic degradation.

Figure 13-58 Molecular Biology of the Cell (© Garland Science 2015)

 b -Coronaviruses Use Lysosomes for Egress Instead of the
Biosynthetic Secretory Pathway
CRISPR
In Brief
Ghosh et al. (2020) provide evidence that endogtoed
b-coronaviruses do not use the
biosynthetic secretory pathway typically urgeby
used by enveloped viruses to leave
infected cells. Instead, these viruses
traffic to lysosomes for unconventional
egress (to exit) by Arl8b-dependent lysosomal
exocytosis. Their non-lytic release results in
lysosome deacidification, inactivation of
lysosomal degradation enzymes, and disruption
of antigen presentation.

O
Will remind you of this in
Lecture 7 as well

Ghosh et al., 2020, Cell 183, 1520–1535