Covid Report

Patient Name : Mr.
SAIBAL SARKAR Collected : 19/Apr/2023 01:15PM

Age/Gender : 39 Y 0 M 0 D /M Received : 19/Apr/2023 08:28PM
UHID/MR No : DSPA.0000001525 Reported : 19/Apr/2023 11:59PM
Visit ID : DSPAOPV2115 Status : Final Report
Ref Doctor : M. M HALDER Client Name : PCC SILPARA
IP/OP NO : Patient location : Kolkata,Kolkata
DEPARTMENT OF MOLECULAR BIOLOGY.

COVID 19
Test Name Result Unit Bio. Ref. Range Method
COVID-19(SARS-CoV 2)-REAL TIME PCR(RT-PCR)

SAMPLE TYPE NASOPHARYNGEAL
SWAB AND
OROPHARYNGEAL
SWAB
SARS-CoV-2 DETECTED
N-Gene CT VALUE 26
ORF1ab Gene CT VALUE 26
SRF ID : 1931503425604
Comment:
Biological Reference Rang
*Ct value < 35 is Positive
*Ct value >35 is Negative
*Ct cutoff suggested by ICMR

Ct value does not correlate with
disease severity
Please Note:
*The cut-off of the reported CT value is as per the manufacturer.
*CT values vary with the type of kit used, the types of samples collected and the various pre-analytical factors.
*The published studies are conflicting to definitively prove a direct correlation between disease severity/infectiousness and CT
values therefore; relying on CT values for determining infectiousness of COVID-19 patients and deciding management protocols
is left to clinical discretion.
Kit used: CoviPath ™ COVID-19 RTPCR KIT
Result Interpretation
Indicates presence of detectable levels
Detected (Positive) Result
SARS-CoV-2 specific RNA (ORF1ab gene and/ or N gene) in patient’s sample.
Indicates absence of detectable levels

Not Detected (Negative) Result
SARS-CoV-2 specific RNA (ORF1ab gene and/ or N gene) in patient’s sample.
Page 1 of 4
Patient Name : Mr.SAIBAL SARKAR Collected : 19/Apr/2023 01:15PM

COVID 19
Indicates the target concentration is too low, or presence of interfering substances

Indeterminate Result
leading to PCR inhibition.
NOTE:
If either of the genes cannot be determined or will give indeterminate results, further testing maybe required using another kit
with a different primer and probe.
A Not Detected (Negative) Resultdo not preclude SARS-CoV-2 and should not be used as the sole basis for patient
management decisions. Kindly repeat test after 48 to 72 hrs if clinically suspected.
Lower respiratory tract specimens are more representative and are preferred.
If at a later date, suspicion of COVID-19 is strong, a fresh sample for Real Time PCR after a gap of 2-4 days after collection
of first sample maybe considered.
Please contact your Physician for necessary action to be taken and to limit the spread of infection.
Clinical Correlation and Correlation with the history of the patient is required before arriving at any conclusion. Presence of
Non-Specific interfering substances during this assay to be kept in mind. Please correlate clinically before arriving at any
conclusion.
False positive and false negative results can be due to multiple factors including sampling technique, transport & interference in
the assay can affect the result.
Presence PCR inhibitors in sample may lead to false negative or invalid results.
Mutation in the target sequence of SARS-CoV-2 or change in the sequence due to virus evolution may lead to false negative
results.
Invalid Result: There is no typical S-shape amplification curve or Ct >35 or No Ct detected for target genes and internal
control, indicating the specimen concentration is too low, or there are interfering substances that inhibit the reaction. If upon
retest, the result is invalid again, another fresh sample should be collected and tested.
SARS-CoV-2 Virus:
SARS-CoV-2 is an enveloped single stranded positive sense RNA virus belongs to family Coronoviridae. It carries largest single
stranded RNA genome of ~30kb and is the causative pathogen of ongoing COVID-19 pandemic (3). The capsid consists of the
nucleocapsid protein N and this is further surrounded by a membrane, that contains three proteins: the membrane protein (M) and the
envelope protein (E), which are involved in the virus budding process, and the spike glycoprotein (S), which is a key player in binding
host receptor and mediating membrane fusion and virus entry into host cells (4).
Page 2 of 4

COVID 19
Disclaimer:
This Test is based on real-time reverse transcriptase PCR technology for the qualitative detection of severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) specific RNA
*ICMR ID- DLUAHTMH-

*ICMR ID-AHLLBHT- HYDERABAD *ICMR ID-AHLVDLA- VIJAYAWADA
MUMBAI
*ICMR ID-ADNTKLKWB -
*ICMR ID-AHLLKDLKAP- KURNOOL *ICMR ID-AHLLDLDD-DELHI
KOLKATA
*ICMR ID-APHLILKDLCTN-
*ICMR ID-AHLLKDIALBK- BENGALURU *ICMR ID-AHHHSPPMH- PUNE
CHENNAI
*ICMR ID-APHLTHLFLADLCK-
LUCKNOW
(For kits determining RdRp gene or ORF1b, sensitivity / specificity of such kits shall be mentioned).
COVID-19 Advisory
Page 3 of 4

COVID 19
*** End Of Report ***
Page 4 of 4
SIN No:MO02887451

Covid Report

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Patient Name : Mr.

SAIBAL SARKAR Collected : 19/Apr/2023 01:15PM

DEPARTMENT OF MOLECULAR BIOLOGY.

COVID-19(SARS-CoV 2)-REAL TIME PCR(RT-PCR)

*Ct cutoff suggested by ICMR

Kit used: CoviPath ™ COVID-19 RTPCR KIT

Indicates absence of detectable levels

DEPARTMENT OF MOLECULAR BIOLOGY.

Indicates the target concentration is too low, or presence of interfering substances

DEPARTMENT OF MOLECULAR BIOLOGY.

*ICMR ID- DLUAHTMH-

DEPARTMENT OF MOLECULAR BIOLOGY.

* End Of Report *

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