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    Insulin Hypoglycemic Effect in Rabbit

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    BlAcK ShArK OP
    Insulin

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    © All Rights Reserved
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    Insulin Hypoglycemic Effect in Rabbit

    Uploaded by

    BlAcK ShArK OP
    11 views3 pages
    Insulin

    Original Title

    Insulin hypoglycemic effect in rabbit

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    © © All Rights Reserved

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    DOCX, PDF, TXT or read online from Scribd

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    Insulin

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    11 views3 pages

    Insulin Hypoglycemic Effect in Rabbit

    Uploaded by

    BlAcK ShArK OP
    Insulin

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Insulin Hypoglycemic Effect in Rabbits Using Alloxan-Induced Diabetes

    Aim
    To evaluate the hypoglycemic effect of insulin in alloxan-induced diabetic rabbits by
    comparing blood glucose levels before and after insulin administration.
    Principle
    Alloxan induces diabetes in rabbits by selectively destroying the insulin-producing beta cells
    in the pancreas, leading to hyperglycemia. Insulin administration is expected to reduce the
    elevated blood glucose levels in these diabetic rabbits. This experiment involves three groups
    of rabbits: a control group, a diabetic group (induced by alloxan), and a diabetic group treated
    with insulin. Blood glucose levels are measured before and after treatment to assess the
    effectiveness of insulin.
    Requirements
     Animals: 9 rabbits (divided into 3 groups of 3 rabbits each)
     Chemicals:
    o Alloxan monohydrate (to induce diabetes)
    o Insulin (to treat the diabetic group)
    o Normal saline (for injection)
     Equipment:
    o Glucometer or glucose assay kit
    o Syringes and needles
    o Weighing balance
    o Blood collection tubes
    o Centrifuge (if needed for plasma separation)
    o Anesthetic (if required for blood collection)
    Procedure
    1. Animal Grouping:
    o Group I (Control Group): Rabbits receive normal saline (no alloxan or
    insulin).
    o Group II (Alloxan Group): Rabbits receive an injection of alloxan
    monohydrate to induce diabetes.
    o Group III (Alloxan + Insulin Group): Rabbits receive an injection of alloxan
    monohydrate to induce diabetes, followed by insulin treatment.
    2. Induction of Diabetes:
    o Group II and Group III: Administer alloxan monohydrate (150 mg/kg body
    weight) intravenously to induce diabetes. After 72 hours, confirm
    hyperglycemia by measuring fasting blood glucose levels.
    3. Insulin Administration:
    o Group III: Administer insulin subcutaneously at a dose of 0.5-1.0 IU/kg body
    weight.
    4. Blood Collection:
    o Collect blood samples from all groups before and after treatment (6 hours
    post-treatment for insulin group).
    o Measure blood glucose levels using a glucometer or glucose assay kit.
    5. Observation:
    o Record the blood glucose levels before and after treatment.
    6. Data Recording:
    o Prepare a table to record the blood glucose levels of each rabbit in all three
    groups before and after the treatment.
    Observation Table (Raw Data)
    Rabbit Blood Glucose Before Blood Glucose After
    Group
    No. Treatment (mg/dL) Treatment (mg/dL)
    Control Group (I) 1 80 82
    2 78 80
    3 79 81
    Alloxan Group (II) 4 230 232
    5 240 238
    6 250 249
    Alloxan + Insulin
    7 235 120
    Group (III)
    8 245 130
    9 255 140
    Results
     Group I (Control Group): Blood glucose levels remained relatively stable before and
    after the treatment, indicating normal glucose metabolism.
     Group II (Alloxan Group): Blood glucose levels were significantly elevated after
    alloxan administration, confirming the induction of diabetes.
     Group III (Alloxan + Insulin Group): Blood glucose levels significantly decreased
    after insulin administration, demonstrating the hypoglycemic effect of insulin in
    alloxan-induced diabetic rabbits.
    Conclusion
    Insulin administration effectively reduced elevated blood glucose levels, confirming its
    therapeutic potential in managing diabetes.

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