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    v4 - Measuring The Size of A Cell

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    v4 - Measuring The Size of A Cell

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    Tutor Virus
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    v4_Measuring the size of a cell (1)

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    6 views2 pages

    v4 - Measuring The Size of A Cell

    Uploaded by

    Tutor Virus

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Title: A study on measuring the size of the specimen using eyepiece graticule (1 Mark; or Exact 0.

    5)

    INTRODUCTION (Define calibration, importance of measuring a cell, common method of measuring


    a cell, introduce the lab). 10 MARKS

    Calibration refers to the process of aligning a microscope’s measurements with a known standard. (2
    Mark)

    Importance of measuring a cell: This information can be used to study cell growth and division, as
    well as the transport of materials in and out of the cell, to understand the properties and function of
    a cell (3 Mark)

    Common methods of measuring a cell: Microscope using instruments such as a Stage Micrometer as
    an accurate ruler and Eyepiece Graticule (3 Mark, if microscope without the instruments 1.5 Marks)
    1 mark for any other method.

    Introduce the lab: This practical is a simple introduction to the use of a microscope and how it can be
    used to take measurements of different cells. (1 Mark)

    AIM (2 Mark)

    To learn how to calibrate a microscope and measure the size of a cell ( Zebrina Plant).

    MATERIAL AND METHODS (5 Mark)

    1. Replace the microscope eyepiece with the eyepiece graticule and ensure the eyepiece
    graticule scale is placed horizontally.
    2. Place the stage micrometer on the stage microscope and ensure it is brought to focus.
    3. Calibrate: Line up the zero of the eyepiece graticule and the zero of the stage micrometer.
    Make sure the scales are parallel. Look at the scales and see where they are in line again. (2.5
    Marks)
    4. Determine what each length on the eyepiece graticule corresponds to in terms of actual
    micrometer measurements on the stage micrometer. (2.5 Marks)

    RESULTS ( Calculation in mm 2.5 Marks and calculation in microns 2.5 Marks )

    Conversion Units: 1mm = 100μm,

    Stage Micrometer: 0.1mm = 1 division,

    1mm = 10 divisions

    Calibration Factor: 10 divisions (Stage Micrometer) = 65 divisions ( Eyepiece graticule) Note: this can
    vary 65-68 divisions.

    : 1mm = 65 divisions

    For Example if cell length is: 4 divisions


    Do other calculations based on the divisions of the cell length you will get in your respective LAB TGs.

    2. Answer 540 μm (2 marks)

    3. Answer 16 divisions (2 marks)

    CONCLUSION (2 marks)

    Should answer to the aim while stating and explaining why the experiment was successful or
    unsuccessful.

    References (2 marks)
    At least two correctly cited and alphabetically arranged references.

    TOTAL 31 MARKS

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