Rojas Et Al 2022
Rojas Et Al 2022
Rojas Et Al 2022
Nutrición en los cultivos de cacao (Theobroma cacao L.): Que factores que
deben ser considerados?
URL: http://portal.amelica.org/ameli/journal/23/233546009/
DOI: https://doi.org/10.24215/16699513e101
Corresponding author: [email protected]
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Carmona-Rojas et al. (2022) Nutrition in cacao crops
Abstract
Cacao (Theobroma cacao L.) is an important commercial crop and agricultural commodity worldwide;
for some Latin American countries, it is an essential part of export products. In Colombia, this crop
has promising conditions to extend and strengthen this agriculture sector.However, their productivity
is low under current agricultural practices, mainly due to insufficient modernization and inadequate or
no management of their nutritional schemes. This publication reviewed the different findings currently
in the scientific literature regarding the factors that determine the nutritional status of cacao plants,
such as the function and distribution of minerals, nutritional efficiency, soil properties, establishment
systems, organic and organic and inorganic sources examined. Additionally, it highlighted the
importance of using and expanding diagnostic tools to determine nutritional needs and the design of
effective programs according to the particular conditions of each region and the genotypes planted.
This conceptual journey highlights the existing theoretical and experimental gap in the identification
of the factors that determine the nutritional status of the plantations and their effect on the
implementation of the fertilization programs used today. Information together provides elements to
adequately address this agronomic practice and the economic impact on farmers and the cacao
production chain.
Resumen
El cacao es un importante cultivo comercial y commodity agrícola a nivel mundial, para algunos países
de Latinoamérica es parte importante de los productos de exportación. En Colombia este cultivo
presenta condiciones promisorias para extender y fortalecer este sector de la agricultura. Sin
embargo, bajo las prácticas agrícolas actuales sus productividades son bajas,debido principalmente
a la insuficiente tecnificación, así como a un manejo inadecuado o nulo de sus planes nutricionales.
En esta publicación se revisaron los diferentes hallazgos encontrados en la literatura científica
disponible a la fecha, respecto a los factores que determinan el estado nutricional de las plantas de
cacao. Entre ellos se examinaron la función y distribución de los minerales, la eficiencia nutricional,
las características fisicoquímicas del suelo, los aportes nutricionales de los sistemas agroforestales
y de fuentes orgánicas e inorgánicas. Igualmente se resaltó la importancia de usar y ampliar las
herramientas de diagnóstico para determinar las necesidades nutricionales y el diseño de programas
efectivos de acuerdo con las condiciones particulares de cada región y los genotipos sembrados.
Este recorrido conceptual deja en evidencia el vacío teórico y experimental existente para la
identificación de los factores que determinan el estado nutricional de las plantaciones y su efecto en
la implementación de los programas de fertilización usados en la actualidad. Información que en
conjunto brinda elementos para abordar de manera adecuada esta práctica agronómica y la
incidencia económica en los cultivadores y la cadena productiva del cacao.
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INTRODUCTION
Theobroma cacao L. (Malvaceae) is a species native to the tropical rainforests of South America
(Zarrillo et al., 2018). This fruit crop reaches its productive age in 3 to 5 years of development, with a life
cycle of between 25 to 40 years (Van et al., 2015). Cacao beans are the primary raw material for
producing food products such as chocolates and confectionery as well as, an emerging and growing
market for cosmetic and pharmaceutical products (Quek et al., 2020). In 2019 the global market value
of the chocolate industry exceeded US$13.58 billion and continued to increase as the population grows,
with expectations of reaching US$15.08 billion by 2024 (Suh & Molua, 2022). Currently, T. cacao is an
important cash crop in many tropical countries (Fountain & Huetz-Adams, 2018). Its production is
concentrated in Africa (63.2%), followed by Asia (17.4%) and Latin America (14.1%), where Ecuador
and Brazil are the major producing countries (Wickramasuriya & Dunwell, 2018).
In Colombia, cacao is grown in 29 of the 32 departments of the country; for the year 2020, 188
thousand hectares of planted areas were registered and distributed in the departments of Santander
(41%), Antioquia (9%), Arauca, Huila (8%), Tolima (7%) and Nariño (5%) (MADR, 2021). The national
average production in the last ten years was 46 thousand tons. However, for the year 2020, it was close
to 63 thousand, distributed mainly in the departments of Santander (26 thousand tons), Antioquia (5
thousand tons), and Arauca (5 thousand tons), which on average register annual productivity of 500
kg.ha-1.year-1 (MADR, 2021). Our country has the ideal agroecological and climatic conditions for
cultivating cacao since it has different thermal floors and is located in the equator belt, a geographical
area with the temperature and humidity conducive to the growth of this plant species (Escobar et al.,
2021). This frame has prompted the National Government and growers to make significant bets on
strengthening a sector with great economic potential and growing demand worldwide (Escobar et al.,
2021).
Despite having achieved significant progress in hectares planted with an increase in productivity
during the last five years, the country only represents 1% of world production, placing Colombia as a
country with low competitiveness in the cacao agricultural chain (Abbott et al., 2018). Among the main
reasons for this productivity are factors such as the advanced age of the crops, the planting of hybridand
common cacao with little tolerance to pests and diseases, the low density of trees per hectare, the small
number of cultivated areas, the lack of tech in crop and inadequate management of the nutritional plans
of the trees (Sánchez et al., 2019).
As one of the main lines of work to face the technical and productive difficulties of this crop, the
intervention in the elaboration of nutritional plans has been proposed considering the genotypes sown
and the edaphoclimatic conditions of the different country regions (National Federation of Cacaoteros
[FEDECACAO], personal communication, May 2021). The nutritional status of plants depends on factors
such as the physical-chemical and biological characteristics of the soil, water availability, climate, crop
management, and the features of each genotype (Van et al., 2015). In Colombia, some of these
characteristics are limiting factors, mainly the low availability of water and acidic and nutrient-poor soils
(León-Moreno et al., 2019).
Fertilizers have traditionally been recommended to overcome soil nutritional deficiency (Snoeck et al.,
2016; FEDECACAO, 2018). Several studies show that formulations that supplemented the primary
nutrients in soils, such as nitrogen (N), phosphorus (P), and potassium (K), have significantly increased
cacao productivity (Puentes-Páramo et al., 2014a; Álvarez-Carrillo et al., 2016). However, even an
additional contribution is made to the soil; plants are not always efficient in using mineral elements, which
directly impacts soil degradation and water pollution. Therefore, in addition to the over cost it generates
in production, it significantly affects the economic viability of productive projects. In Colombia, cacao
production has been classified as a peasant economy: small and medium producers. Around 52,000
families are part of this agricultural chain. They mainly belong to a low social stratum, with an explicit
limitation for the implementation of optimal fertilization programs and sustainable framing practices for
crop management. This context admits the need to implement different biotechnological strategies to
improve crops' nutritional status. They could including the selection of elite materials, the improvement
of plant material, and efficient clonal propagation systems, with solutions framed in our socioeconomic
context that tend towards a more profitable, productive, environmentally and economically sustainable
agricultural system.
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Carmona-Rojas et al. (2022) Nutrition in cacao crops
Considering the importance of nutrition in cacao crops, the objective of this publication was to review
the different findings published to date in the scientific literature regarding the factors that determine the
nutritional status of cacao crops, emphasizing the publications reported on the countryand its neighboring
countries. Besides analyzing the nutritional contributions of fertilization programs and the importance of
adequate diagnosis. Information that together seeks to provide elements for a more critical and in-depth
understanding of the proper management of nutrition and its possibleeconomic impact on cacao farmers.
It also encourages the implementation and development of research programs that provide the elements
for the sustainable increase of crop productivity and provides bases to intensify research efforts in this
area of knowledge.
In forming the plant structure in cacao trees, the absorbed nutrients are destined to develop and
consolidate their different vegetative parts, such as roots, trunk, branches, and leaves. The absorptionof
nutrients of greater consumption, such as N, Ca, K, P, and Mg, increases in the first five years of planting
until they reach full vegetative development; from this stage, the nutrients translocated for the flowering
and fruiting of the plant (Salvador et al., 2012; Romero, 2018).
In cacao plants, the nutrient content differs significantly in the tree parts, finding that it is higher in the
leaves and pods, while the stem and branches show lower values (Leiva-Rojas & Ramírez-Pisco,2017;
Romero, 2018). Different works agree on how nutrients are distributed in cacao leaves. Among these
investigations, Leiva-Rojas and Ramírez-Pisco (2017) analyzed some cultivars in Colombia,the following
distribution of nutrients in the leaves, N: 1.4% - 2.2%; K: 1.2% - 2.2%; Ca: 0.8% - 2%, Mg: 0.33% - 0.9%,
P: 0.13% - 0.20% and S: 0.07% - 0.27%. Based on the above analysis, the authors propose the following
order of content, N ≥ K ≥ Ca > Mg > P> S, making the proviso that this can change depending on the age
of the crop. In this sense, Puentes-Paramo et al. (2016a) reported that this order can be variable
depending on the genotype; for example, for the genotypes ICS-95 and ICS-39, this order of content
Ca>N>K>Mg was found; while in TSH-565 the following order N,>Ca>K>Mg was presented, which is
reversed for K and Ca in the genotype CCN-51. All the genotypes studied showed a very similar affinity
for the rest of the nutrients (P, S, Mn, Fe, Zn, Na, B,and Cu). Previous work suggests that the elements'
distribution values may vary depending on the planting material. However, those required in a more
significant proportion for the vegetative growth of trees are N, K, and Ca.
The pods, including the bean and the husk, represent the most significant nutrient extraction for cacao
plants. Different studies have calculated the number of nutrients removed per 1000 kg of dry grain (Table
1). Showing on average that the seeds remove in greater quantity N, in a range between 20 kg.kg-1 and
24 kg.kg-1, and it is suggested in general terms an order of extraction as follows,
N>K>P>Mg>Ca>Zn=Mg>Fe>Cu>B. On the contrary, the analyses that also consider the shell reportthat
K is the nutrient found in the highest concentration, ranging between 45 kg.kg-1 and 50 kg.kg-1 (Table
1). This means that high amounts of K are required to format the fruit. Therefore, it is the element that
must be added to the soils in greater proportion during the production stage.
Regarding the function of mineral elements in plants, it is known that the 16 elements considered
essential play different biochemical and biological roles for plant species (Maathuis & Diatloff, 2013).
Furthermore, some nutrients have specific functions in cacao plants. In most crops, nitrogen is one of
the nutrients required in the in the most significant quantity for cacao. It is essential for the vegetative
growth of trees, drives the development of branches and leaves, and represents the highest percentage
in the almond (Puentes-Páramo et al., 2014a; Furcal-Beriguete, 2017). Its deficiency reduces the leaf
formation rate and accelerates defoliation, so an adequate supply of this element helps combat the
progressive death field (Santana & Cabala-Rosand., 1982). It should be noted that mature trees can only
respond to N when pruned (van Vliet et al., 2015).
Phosphorus is necessary for developing roots, wood, young shoots, and flowering. The deficiency of
this element generates small plants, narrow leaflets, and early and severe defoliation. The application of
P in cacao increases the flowering (Asomaning et al., 1971) and dry matter production (Ofori et al., 2017).
On the other hand, it has been found that there is an interaction between N and P, where a greater radius
of N/P increases cacao yields, and the lack of response to the supply of N may be due to the scarcity of
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P and vice versa, a response that is mainly due to the affectation in the development of the roots to the
deficiency of this element (Wessel, 1971; Puentes-Páramo et al., 2016a).
For its part, potassium is of great importance in the physiological processes of cacao plants,
particularly for the development and pods maturation, which accumulate large amounts of this element,
so the quality of cacao depends mainly on the supply of field (Furcal-Beriguete, 2017). Furthermore, K
comprises about 70% of the minerals in the xylem sap of cacao and is found in branches, stems, and
roots, turning these into reserve organs, which the plant can access to take K when needed. Additionally,
its deficiency generates greater susceptibility to water deficit (Wang et al., 2013). In experiments on
cacao seedlings, it was found that sodium (Na) could partially replace K, with beneficial effects on
photosynthesis and water use efficiency. This further suggests that Na might be more efficient than K in
the closing and opening stomata process, leading to lower susceptibility to drought conditions (Gattward
et al., 2012).
Calcium is essential for the development and vigor of stems, young roots, shoots, and terminal
flowers. It increases the shelf life of harvested fruits and organs (Jadin & Snoeck, 1985, cited by Snoeck
et al., 2016). Malavolta et al. (1997) describe that high levels of Ca in the soil increase the foliar
concentrations of Ca, Fe, Zn, and Mg, but decrease other nutrients such as N, Mn, and Na. Magnesium,
this element is indispensable for photosynthesis. Particularly in cacao, a prolonged period of magnesium
deficiency causes older leaves to fall off while young leaves are not affected, causing trees to defoliate
quickly (Chepote et al., 2013).
Finally, among macronutrients, commercial formulations for cacao contain sulfur. For cacao, there
needs to be precise information on the requirements and application of this element. López & Lucas
(2018) included in their research the application of concentrations of S and other mineral elements,
finding improvements in the response of trees to primary diseases and crop productivity. It is essential
to point out that this element in our region needs to be understood since the requirements have not been
established yet.
More information needs to be published to understand the influence and importance of micronutrients
in cacao nutrition, and their deficiencies, which are generally not identified in the crops (Cruz et al., 2015).
Micronutrient deficiencies in the soil are not only presented by the scarcity of the different elements, but
also by factors such as pH or high concentrations of other minerals that affect their bioavailability (Bonilla,
2008). For cacao, some work has been carried out to recognize the importance of these elements. For
example, Boron is crucial for forming cacao fruits, and the application of borax (4.4g/tree) generated an
increase in yields by 180% in a cacao plantation on Ivory Coast (Snoeck et al., 2016). Furthermore,
cacao trees have generally been reported to have minimum threshold of 0.2 mg/kg of soil-soluble B.
Further suggesting that soil analyses are more reliable than foliar analyses in determining a deficiency
of this nutrient B-deficient cacao soils showing reductions in yield of up to 40%, with the presence of
deformed fruits, short internodes, brittle leaves, and reduced seed size; in addition to greater
susceptibility to black pod disease (Lachenaud, 1995).
On the other hand, Zinc is a crucial element for the activity of several enzymes. It has been shown to
have a differential impact on the growth of cacao seedlings and the accumulation of other elements at
the foliar level, such as P, K, Ca, Mg, Mn, Fe, Zn, and Cu. However, high concentrations of this element
can cause a reduction in plant growth (Cruz et al., 2015).
Based on the work made in cacao so far, it is possible to approach the understanding of the
participation of each of the nutrients, considering their participation in the building of the different plant
organs. They also provide valuable elements to answer the question of how much the plant needs to
build its biomass, foliage, roots, and fruits. However, it is a complex parameter that requires the
integration of different factors such as genotype, edaphoclimatic characteristics, agricultural practices,
etc., which allow the consolidation of a more accurate profile.
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Table 1
Nutrient concentration in seeds and pods for different cacao genotypes grown in different regions of
Colombia and America.
For seeds the concentration of each element is based on 1000 kg of dry grain. The values presented in
the table are those obtained in the best fertilization program in each of the studies. bThe values presented
in the micronutrients for this clone are expressed in mg/kg. cNutrient extraction for one tone of dried seeds
including cob remains.
NUTRITIONAL EFFICIENCY
Nowadays, the bet is to look for environmentally friendly production technologies to improve the
competitiveness of the cacao chain. One of the alternatives is to use the genetic resources available to
the country to identify, characterize and select materials tolerant to nutritional stress. It has been
identified that during the domestication of plant species and varieties, they have differences concerning
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nutritional requirements. From an agronomic approach, it has been translated to the efficiency of using
nutrients, an essential characteristic for selecting and improving elite varieties (Shin, 2014; Wang & Wu,
2015). Nutritional efficiency is defined as the maximum yield that can achieve by growing in nutrient-poor
soils (Reich et al., 2014). These nutrient-efficient plants can absorb more nutrients from the soil (uptake
efficiency) and produce more dry biomass per unit of the absorbed nutrient (utilization efficiency) (Reich
et al., 2014).
Few nutritional studies for cacao plants have been carried out to date. These studies show that certain
genotypes can be more efficient in taking N, P, a n d K from the soil and respond differentially to
fertilization programs, achieving in some genotypes optimal growth and high yields inproduction (Ribeiro
et al., 2008; Puentes-Páramo et al., 2014b; Li et al., 2015; Cuenca-Cuenca et al., 2019; Rosas-Patiño et
al., 2019). In the same line as these studies, Ofori et al. (2017) demonstrated that several cacao
genotypes have physiological differences that allow them to develop well in marginal conditions, with low
irrigation and nutrient scarcity, managing to identify potential varieties for future genetic improvement
programs; despite not determining nutritional efficiency.
These studies set remarkable aspects to study the depth of the nutrition component. Nutritional
efficiency has yet to be identified and characterized for selected Colombia's cacao genotypes as cultivars.
There needs to be more information on the molecular, cellular, and physiological processes associated
with nutrient uptake, translocation, and use. Such information is necessary for selecting clones and
developing future genetic improvement programs. The selection of new clones and the cultivation of
nutrient-efficient genotypes represent a sustainable approach to the nutritional management of
agricultural systems, which aims to reduce soil degradation, minimizing the application of chemicals,as
a commitment to a cleaner and more environmentally friendly production. Look for increased crop yields,
but with economically viable technologies that benefit both small and large producers. It could be one of
the strategies to make cacao crops feasible as a business model that promotes the economy of the
agricultural sector in our country.
The nutritional status of plants depends mainly on the physical, chemical, and biological properties of
soils (van Vliet et al., 2015). For cacao trees to grow appropriately and generate maximum productivity,
plants must form a broad root system, with thick root and lateral roots found in the upper20cm of the soil,
which is the primary pathway for nutrient and water uptake (Hartemink, 2005).To establish a productive
orchard in Colombia, it has been suggested to select soils with physical- chemical parameters close to
those determined as appropriate for optimal crop development (Table 2, Table 3).It should be noted that
these values may vary slightly depending on the edaphoclimatic conditions of each region, as well as
the genotype sowed (Rosas-Patiño et al., 2019).
In Colombia, cacao crops are present in several departments, with notable differences in soil types,
some of wich have characteristics close to those appropriate for the cultivation of the species. While in
others, it is necessary to apply amendments, fertilizers, and lime to improve the structure of the soil,
replace some nutritional deficiencies, and correct pH values.
The optimal soil properties reported to date point to adequate management based on clear criteria for
its agricultural destination and financial sustainability of long-term productive projects for cacao crops.
However, information in this area is not enough, given the wide range of agroecological environments in
which crops grow in Colombia (García et al., 2004). These shortcomings in the information raise the
importance of increasing and deepening studies that expand the characterizations of Colombian soils.To
date, the impact of different management practices in response to cacao crop yields has been evaluated,
especially using elite regional materials that have demonstrated important agronomic characteristics but
have not yet been addressed in this type of research (FEDECACAO, 2018).
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Table 2
Optimal physicochemical properties of the soil for proper development of cacao crops in Colombia.
* Reference values for Colombia suggested by the National Federation of Cacao Growers (FEDECACAO,
2013; FEDECACO, 2018), some values were specified with other bibliographic sources (Snoeck et al.,
2016).
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species that accompany the crop. Similarly, other authors indicate that thenutritional status of cacao trees
is significantly modified by interactions with shade trees (Isaac et al., 2007). One element that demands
greater concentration in plant species, including cacao, is N, so it is recommended to use as shade
species of legumes fixing gaseous nitrogen (N2). This practice can significantly contribute to this element
and generate a good proportion of deposition of leaves, branches, and flowers, increasing the
accumulation of organic matter on the surface and favoring microfauna. For example, Inga edulis Mart
(Fabaceae) was found to have an N2 fixation rate of 41 kg.ha-1.year-1.This plant directly transfers nitrogen
to the cacao roots through a common mycelial network of mycorrhizal fungi and recycling N-rich root
exudates that generate an average contribution of 50 kg.ha-1.year-1 of the N present in the cacao roots
(Nygren & Leblanc, 2015).
Another essential element is P, Aleixo et al. (2017) found that there is a significant accumulation of
total inorganic and organic phosphorus in cacao crops associated with Erythrina spp (Fabaceae),
compared to cabruca-plus-cacao-type forest cover; with an average amount of P in the labile fraction of
51 kg.ha-1 and 33.5 kg.ha-1, respectively. A similar result was obtained with crops associated with Hevea
brasiliensis Müller Argovi (Euphorbiaceae), which accumulated 41 kg.ha-1 of P in the labile fraction
amounts to replenish the nutrients exported by cacao seeds (Table 1). This evidence suggests that the
different plant species that make up agroforestry systems have the potential to supply the P required by
cacao plants through the mineralization process in case of suppression of phosphate mineral fertilization
(Aleixo et al., 2019). These studies together point to the importance of this agricultural practice being
developed under a solid ecological foundation, aiming to maintain an adequate nutritional balance for
the entire system.
Table 3
Reference levels of different physicochemical parameters for cacao soils in Colombia and other regions of
the world.
aMinimum values of chemical conditions in Colombian soils for the development of cacao production
projects. bAccording to the classification of authors, these values correspond to moderately suitable
characteristics. cPercentage of saturation of Al. d, fRecommended values based on different studies
worldwide, which may vary according to each locality and genotype. e Values obtained by Mehilich/Olsen
extraction methods. g Organic matter in g.Kg-1.
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CHEMICAL FERTILIZATION
In Colombia, researches on soil and foliar fertilization in cacao is scarce and is in full development
and increase due to the recent recognition of the importance of this crop for the country. Table 4 shows
fertilization programs implemented in our country and other Latin American countries. Here, it is observed
differences in the amounts and sources of nutrients, as well as the yields obtained according to the
genotype. Such results obey the various factors that impact this agronomic practice.
The first action usually implemented in a fertilization program is the management of acidity, which
controls the availability of nutrients. It is also essential, especially in the soils of the tropics, where acidic
soils predominate (Casierra, 2007). This management is carried out through the application of lime to
increase pH values, mitigate the toxicity of aluminum, and ensure the availability of elements such as Ca,
Fe, Mg, and P. Among other benefits that allow for the gradually restoring the soil's fertility in its chemical
composition (Castro & Guerrero, 2018). An example of the effect of liming is the study done by Rosas-
Patiño et al. (2017, 2019) in the Colombian Amazon's oxisol, ultisol, and entisol soils. The additions of
amendments significantly improved soil characteristics, including nutrient availability and increasing
cacao crop yields.
Once it is possible to balance the acids of the soil, the next step is to complement the missing nutrients
in it. For cacao, the fertilization programs implemented have generally been designed to compensate for
the demands of N, P, and K (Table 4). Reported studies have mostly found that the application of these
elements increases production (Uribe et al., 1998; Rosas-Patiño et al., 2019), but in others, there was
no significant increase in productivity (Sánchez et al., 2005).
Differential responses to implemented fertilization programs may be due to factors such as difficulties
in adopting other agronomic practices, added to an imbalance in the nutrients added. Somework is forceful
in demonstrating that if fertilization is exceeded, lower yields are obtained due to theantagonism between
the elements, which reduces the absorption of other nutrients. For example, if N is added as a source of
ammonium, the absorption of other cations such as Ca2+, Mg2+ or K+, can be reduced (Puentes-Páramo
et al., 2014b; Cuenca-Cuenca et al., 2019).
Another factor that conditions the response of nutritional programs is the genetic potential and
physiological and morphological characteristics of each genotype, which in turn is influenced by the
edaphoclimatic conditions where the crop grows. A clear example is the results obtained in two
investigations where the same fertilization program was implemented, and identical genotypes were
evaluated. However, a marked difference in yields was obtained, where the most significant
differentiating factor was the edaphoclimatic conditions where these crops grew (Puentes-Páramo etal.,
2014b; Rosas-Patiño et al., 2019). In many crops, despite an adequate balance of nutrients, the
response is genotype-dependent, of which in particular, its yields grow exponentially in different
fertilization programs, which in addition to other characteristics of the genotype, adds the ability to self-
fertilize, ensuring floral fertility and therefore fruit formation (Ruales-Mora et al., 2011).
In fertilization programs, physicochemical characteristics of each of fertilizers used is essential. For
most plant species, including cacao, the nutrients most plants require are N, P, and K, which are the
main elements found in fertilizers. Therefore, it is crucial to understand the most efficient way they are
available to the plant. Generally, to supply the N, urea is applied, a very economical source of this element
(Table 4). However, in Colombia acidic soils are predominant, whereby there might be better options
than urea, given its high volatility and increase in soil acidification. For this reason, it is essential to
consider other sources, such as ammonium sulfate ((NH₄)₂SO₄) or nitrates (e.g., calcium nitrate,
Ca(NO3)2), which are directly available to plants and favor the uptake of other nutrients, with a lower
impact on soil pH (Snoeck et al., 2016). However, its importation causes higher costs and is unprofitable.
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Regarding the sources of P used for cacao, among them are the phosphoric rock (Ca3(PO4)2CaF2
and the triple superphosphate Ca(H2PO4)2·H2O (Table 4), which are good sources of P and also increase
the pH of the soil. However, some studies indicate that care should be taken in the form and amount in
which this element is added, taking into account the pH value and the proportion of K-Ca-Mg found in a
particular soil. These phosphoric fertilizers rich in CaO can create an imbalance in the proportion of
several cations and even precipitate the same P, forming calcium phosphates.
On the other hand, K is the nutrient added in greater concentration due to its high demand for the
formation of the cacao fruit. Therefore, due to its low costs, one of the most used sources in crops is
potassium chloride (KCl). However, Cl can be phytotoxic and, contributes to soil acidification due to its
easy leaching (Snoeck et al., 2016). Other common sources of this element are K2SO4 and KNO3; both
can be excellent sources of K and other nutrients such as S and N. In addition, these sources do not
interfere with the absorption of other ions and have high solubility. Nevertheless, these K sources are
more expensive and can increase the cost of production.
Finally, although macronutrients N, P, and K are the elements that are added in a more significant
proportion in fertilization programs, the other macroand micronutrients are of equal importance at the
physiological level. Unfortunately studies evaluating the impact of micronutrients on cacao crop yields
are scarce. For example, supplementing elements such as B and Zn significantly increasing yields in
different cacao genotypes (Ludeña-Davila, 2013; Cedeño et al., 2017). It also demonstrates that B
deficiency limits the complete filling of the fruits, affecting the cob index, a key parameter with which crop
productivity is evaluated (Lachenaud, 1995).
ORGANIC FERTILIZATION
Another important source of nutrients to meet the nutritional demands of cacao are organic fertilizers,
which are a significant source of nutrients for this crop. Different soil properties improve themselves,
such as the rate of water infiltration, aeration, and moisture retention capacity, among other
characteristics, which facilitate the growth of the root system and, transport nutrients to the plant (Diacono
& Montemurro, 2010). Although organic fertilizers are less soluble and have a slow decomposition, it is
not inconvenient for perennial species such as cacao, which can take advantage of the nutrients that
gradually solubilize in the soil solution.
Organic matter addition is calculated from the amounts of nutrients provided by organic compounds.
High volumes are generally required to exercise their function as soil improvers and provide nutrients. In
several cacao-producing regions, such as Côte d'Ivoire, Indonesia, and Peru, the application of high
amounts of cob shell compost has been shown to represent an essential contribution to the recycling of
nutrients in the system, with a direct impact on increasing crop yield. For example, a study conducted in
oxysol soils in Brazil showed that using 8 tons/ha/year of cacao husk compost promoted a 133% increase
in dry grain production compared to treatment without fertilization (Chepote, 2003). Likewise, the
application of 4 kg.plant-1.year-1 of a mixture of cacao cob shell, livestock manure compost, and 50%
mineral fertilizer (13%N, 35%P2O5, and 10% K2O) promoted an 188% increase in production.
For Colombia, there are few studies where organic fertilizers are evaluated replace chemical fertilizers.
For example, Álvarez et al. (2016) demonstrated an increase in the height and diameter of the stem for
cacao plants at the growth stage. It was obtained with an organic fertilizer consisting of banana stem,
cane bagasse, and bovine manure, among other elements, which were an good source of nutrients for
cacao plants.
Compiling the works in both types of fertilization, chemical and organic, allow us to demonstrate that
fertilization must be evaluated integrally which is essential considering the crop yields and the availability
of nutrients. Furthermore, the impact of these nutrient sources might generate on the soil, biota, the
environmental impact and the economic expenses t each represents. Therefore, this consideration aims
to reduce dependence on external inputs and maintain a balanced ecosystem with high crop yields.
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nursery stage might ensure optimal physiological and sanitary characteristics and good performance in
the field. One of the first works carried out in nutrition was done by Thong & Ng (1980), whom designed
a fertilization program for this stage of the crop. In a recent study conducted by Fernández et al. (2016),
with seedlings of the IMC-67 rootstock, the same nutritional plan of Thong & Ng (1980) was evaluated.
The authors found overfertilization of the seedlings, demonstrating that supplementing only with 50% of
N and K concentration suggested in the previous work could achieve a more significant amount of
biomass. Other studies indicate adding high amounts of some of these nutrients. However, in light of the
additional research carried out on the subject (Table 5), it is considered that some concentrations of the
mineral elements could be toxic to plants at this stage of development.
Based on the above, there is a lack of consensus regarding the nutritional plans for cacao plants in
this stage of development. Such a fact may be due to several factors, including the specific nutritional
demands of each genotype, the type of substrate used, and the differences in environmental conditions
in the greenhouse. Likewise, a deep knowledge of the nutritional dynamics of macro and micronutrients
is required. To date most studies focusing on the evaluation of N, P, and K. Which has led to leaving
aside crucial nutrients such as Zn, Mg, and B at this stage of development, as has been shown in different
studies (Sodré et al., 2010; Ludeña-Davila, 2013; Cruz et al., 2015). These shortcomings of knowledge
in this area make a call to encourage the development of research that allows determining, among other
factors, the appropriate nutritional formulation for this stage of development, the type of substrate,
container, and the requirements of light, temperature and wet.
Table 5
Nutritional requirements of cocoa in the nursery stage determined in different studies.
aNutrient concentrations in g per plant, these dates are one approximation and were calculated from the
data published in each study. b Values presented are expressed as a percentage.
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Carmona-Rojas et al. (2022) Nutrition in cacao crops
Traditionally, tools such as foliar nutritional concentration and the physicochemical parameters of the
soil have been used to know the nutritional status of crops. These tools contribute to understanding the
nutrient dynamics in the soil, plant nutrient uptake, and their use in different physiological processes,
which has served as the basis for the design of fertilization programs. Currently, there are available new
approaches with outstanding contributions due to their novelty and the inclusion of various variables.
Among them, we find the soil quality index, a more holistic and in-depth analysis of soil characteristics
and their relationship to the ecosystem. Likewise, mapping for the identification and management of crop
areas, as a tool for the identification and delimitation of areas in the same crop, which have differences
in their fertility, provides instruments to do precision agriculture.
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Table 6
Ranges to interpret foliar analyses in cocoa. The values presented represent the concentrations in which
each of the elements must be found in the leaf tissue to consider that there is no deficiency or toxicity of
the element in the plant.
These reference values have been determined in the plantations that demonstrate the highest
productivity. Table constructed from the information reported by (Puentes-Páramo et al., 2016b) and other
authors.
From the point of view of farmers, it is found that many need more technical advice for theinterpretation
of soil analyses, which has led them to implement fertilization programs without this information,
assuming an imbalance in nutrition programs and cost overruns in production. This problem has
motivated some researchers to develop software that facilitates understanding and interpreting these
results. To allow the formulation of balanced nutritional programs for cacao crops, consider different
parameters, among them the nutrients exported in each harvest (Snoeck et al., 2007).
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Carmona-Rojas et al. (2022) Nutrition in cacao crops
design of fertilization programs based only on average values can lead to errors in implementing
generalized nutritional programs for the entire plantation (Silva et al., 2010). Determining the variability
within a cultivation area allows the determination of management zones and, therefore, the application
of precision agricultural techniques. Carvalho et al. (2016) validated the methodology for determining
management zones within the same cacao plantation. They established grouping patterns between the
soil properties and the yield obtained by each crop area. Among the variables that provided more
information in this study was the yield of early harvest cacao and the total fractions of sand or clay in the
soil with which the management zones are determined. Similarly, other authors have used soil maps to
demarcate the best agroecological zones to grow cacao, linking crop requirements with soil data and
thus designing accurate fertilizer recommendations (Snoeck et al., 2010; N'Guessan et al., 2017).
Similar studies have yet to be carried out in Colombia. However, the Zoning map of suitability for
commercial cacao crops (Agricultural Rural Planning Unit [UPRA], 2019) was recently published. This
study aimed to design a tool for consultation and identification of areas with an aptitude for establishing
this crop. Furthermore, establish guiding policies for the development of the cacao sector, framed in the
sustainability, competitiveness, and productivity of the cacao chain.
CONCLUSIONS
Cacao cultivation has been identified as a product of excellent projection in international agricultural
markets and a good source of income for hundreds of families in Latin America, including Colombia. This
review has made it possible to identify that developing an effective nutritional plan is a determining factor
in improving the productivity and quality of cacao crops. It is a complex task that requires the commitment
of all actors in the production chain. The studies reviewed made it possible to identify the nutritional
requirements of a crop. However, the impact of fertilization programs on crop yields is strongly influenced
by several factors such as genotypes' genetics, edaphoclimatic characteristics of each area, agroforestry
species accompanying the crop, and agronomic practices used. All those elements suggest that there is
no single fertilization program.It also should be designed with specific criteria for each plantation, using
traditional tools such as foliar and soil diagnosis and other parameters with more profound and holistic
analyses such as the soil quality index, and crop mapping, among other tools.
Likewise, the different works collected in this review highlight the theoretical and experimental gap
between the other aspects that determine the nutritional needs of cacao crops and their effect on the
programs implemented. . Whereby works in these aspects could contribute significantly to establishing a
profitable and sustainable production model for cacao producers.
From the technical and scientific perspectives addressed, it has been possible to identify that, although
there are specific interventions in the processes of fertilization, advice, and delivery of genotypes and
elite materials to growers. These interventions have yet to manage to generate sufficient modernization
of the crop. Integrating actors, such as government, private companies, and universities, is fundamental
for this process. Additionally, the intervention of federations, cooperatives, and other associative
schemes is critical in distributing resources and technical advice for implementing nutrition plans.
Programs will be effective with the commitment of decision-makers and producers to increase their
efforts in changing traditional ways of managing the crop.
Disclaimers
All authors made significant contributions to the paper, agree with its publication, and state that there
are no conflicts of interest in this study.
Acknowledgments
This work was funded by the General Royalty System - Science, Technology and Innovation Fund of the
Government of Antioquia, University of Antioquia, Catholic University of the East, National Chocolate
Company with the project identified with BPIN 2016000100060.
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BIBLIOGRAPHY
Abbott, P.C.; T.J. Benjamin; G.R. Burniske; M.M. Croft; M.C. Fenton; C.R. Kelly; M.M. Lundy;
F.R. Camayo & M.D. Wilcox (2018). An Analysis of the Supply Chain of cacao in Colombia. United
States Agency for International Development 1-69.
Abreu-Junior, C.H. (1996). Foliar nutrient concentration and rations in height yield cocoa genotypes
and relations yield and intensity of witches broom disease. Cacao Research Cocoa Producer ́s
Alliance. Conference. CEPLAC/ CEPEC. Atas.
Aikpokpodion, P.E. (2010). Nutrients Dynamics in Cocoa Soils, Leaf and Beans in Ondo State,
Nigeria. Journal of Agricultural Sciences 1(1): 1-9.
Aleixo, S.; A.C. Gama-Rodrigues; M.G. Costa; M.V. Sales; E.F. Gama-Rodrigues & J.R.B.
Marques (2017). P transformations in cacao agroforest soils in the Atlantic forest region of Bahia,
Brazil. Agroforestry Systems 91(3): 423-437.
Aleixo, S; A.C. Gama-Rodrigues; E.F. Gama-Rodrigues & J. Schripsema (2019). Organic
phosphorus of soils under cacao agroforests in the Atlantic coast of Brazil. Geoderma Regional 17:
e00220.
Álvarez-Carrillo, F.; J. Rojas Molina & J.C. Suárez Salazar (2016). Contribución de esquemas de
fertilización orgánica y convencional al crecimiento y producción de Theobroma cacao L., bajo
arreglo agroforestal en Rivera (Huila; Colombia). Corpoica Ciencia y Tecnología Agropecuaria
16(2): 307.
Appiah, M.; K. Ofori-Frimpong & A. Afrifa (2000). Evaluation of fertilizer application on some
peasant cocoa farms in Ghana. Ghana Journal of Agricultural Science 33(2).
Araujo, Q.R.; V.C. Baligar; G.A.H. de A. Loureiro; J.O. de Souza Júnior. & N.B. Comerford
(2017). Impact of soils and cropping systems on mineral composition of dry cacao beans. Journal
of Soil Science and Plant Nutrition 17(2): 410-428.
Araujo, Q; D. Ahnert; G. Loureiro; J. Faria; C. Fernandes & V. Baligar (2018). Soil quality
index for cacao cropping systems. Archives of Agronomy and Soil Science 64(13): 1892-1909.
Asomaning, E.; R. Kwakwa & W. Hutcheon (1971). Physiological studies on an Amazon shade and
fertilizer trial at the Cocoa Research Institute, Ghana. Ghana Journal of Agricultural Science
4: 47-64.
Ávila-Lovera, E.; I. Coronel; R. Jaimez; R. Urich; G. Pereyra; O. Araque; I. Chacón & W. Tezara
(2016). Ecophysiological traits of adult trees of criollo cocoa cultivars (Theobroma cacao L.) from
a germplasm bank in Venezuela. Experimental Agriculture 52(1): 137-153.
Bai, S.; S.J. Trueman; T. Nevenimo; G. Hannet; P. Bapiwai; M. Poienou & H.M. Wallace (2017).
Effects of shade-tree species and spacing on soil and leaf nutrient concentrations in cocoa
plantations at 8 years after establishment. Agriculture. Ecosystems and Environment 246(June):
134-143.
Baligar, V.C.; J.A. Bunce; R.C.R. Machado & M.K. Elson (2008). Photosynthetic photon flux
density; carbon dioxide concentration and vapor pressure deficit effects on photosynthesis in
cacao seedlings. Photosynthetica 46(2): 216-221.
Bonilla, I. (2008). Introducción a la nutrición mineral de las plantas. Los elementos minerales. In:
Fundamentos de fisiología vegetal. J. Azcón-Bieto & M. Talón., Ed. Mcgraw-Hill -Interamericana
de España, España. pp.103-121.
Borden, K.A.; S.C. Thomas & M.E. Isaac (2020). Variation in fine root traits reveals nutrient- specific
acquisition strategies in agroforestry systems. Plant and Soil 453: 139-151.
Carvalho, P.S.M.; L.B. Franco; S. de A. Silva; G.A. Sodré; D.M. Queiroz & J.S. de S. Lima (2016).
Cacao crop management zones determination based on soil properties and crop yield. Revista
Brasileira de Ciencia Do Solo 40: 1-17.
Casierra, F. (2007). Estrés por aluminio en plantas: reacciones en el suelo, síntomas en vegetales
y posibilidades de corrección, una revisión. Revista Colombiana de Ciencias Hortícolas 1(2): 246-
257.
Castro, H. & J.C. Guerrero (2018). Evaluación de materiales de encalado mediante pruebas de
incubación en un oxisol de la altillanura colombiana. Revista de Ciencias Agrícolas 35(2): 14- 26.
Cedeño, L.; A. Diogenes; V. Calderón & E. Jair (2017). Efectividad de varias combinaciones de
17
Carmona-Rojas et al. (2022) Nutrition in cacao crops
nitrógeno, azufre, zinc, manganeso, boro y fitohormonas sobre el rendimiento y rentabilidad del
cacao nacional. Ing. Thesis. Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix
López. Calceta, Ecuador. pp 11-20.
Chepote, R.E. (2003). Efeito do composto da casca do fruto do cacau no crescimento e produção do
cacaueiro. Agrotrópica (Brasil) 15(1): 1-8.
Chepote, R.E.; G.A. Sodré; E.L. Reis; G.R. Pacheco; P.C.L. Marrocos & R.R. Valle (2013).
Recomendações de corretivos e fertilizantes na cultura do cacaueiro no sul da Bahia: 2a
aproximação. R. Costa Argôlo., Ed. Ministério Da Agricultura, Pecuária E Abastecimento.
CEPLAC/ CEPEC. Boletim Técnico 203.
Cruz, R.; J. Souza; G. Sodré & V. Baligar (2015). Growth and nutrition of cacao seedlings
influenced by zinc application in soil. Revista Brasileira de Fruticultura 37(4): 1053-1064.
Cuenca-Cuenca, E.W.; Y.J. Puentes-Páramo & J.C. Menjivar-Flores (2019). Efficient use of
nutrients in fine aroma cacao in the province of Los Ríos-Ecuador. Revista Facultad Nacional de
Agronomia, Medellin 72(3): 8963-8970.
Diacono, M. & F. Montemurro (2010). Long-term effects of organic amendments on soil fertility.
A review. Agronomy for Sustainable Development 30(2): 401-422.
Escobar, S.; M. Santander; M. Zuluaga; I. Chacón; J. Rodríguez & F. Vaillant (2021). Fine cocoa
beans production: Tracking aroma precursors through a comprehensive analysis of flavor
attributes formation. Food Chemistry 365: 130627.
FEDECACAO (2013). Guía ambiental para el cultivo del cacao. pp. 1-126. Availablein:
http://hdl.handle.net/20.500.12324/11622 Last accessed October of 2022.
FEDECACO (2018). Guía técnica para el cultivo del Cacao. pp. 169-192. Available in:
http://hdl.handle.net/20.500.12324/11684. Last accessed October of 2022
Fernández, L.; J.C. Bohórquez & A. Rodríguez (2016). Dinámica nutricional del cacao bajo
diferentes tratamientos de fertilización con N, P y K en vivero. Revista Colombiana de Ciencias
Hortícolas 10(2): 367-380.
Fountain, A. & F. Huetz-Adams (2018). Cocoa Barometer. Available in:
https://www.voicenetwork.eu/2018/04/2018-cocoa-barometer-released/. Last accessed July of 2022.
Furcal-Beriguete, P. (2017). Extracción de nutrientes por los frutos de cacao en dos localidades en
Costa Rica. Agronomía Mesoamericana 28(1): 113-129.
Garate, A. & I. Bonilla (2008). Nutrición mineral y producción mineral. In Fundamentos de fisiología
vegetal. J. Azcón-Bieto & M. Talón., Ed. Mcgraw-Hill - Interamericana de España, España. pp.
124-143.
García, J.; M. Romero & L. Ortíz (2004). Caracterización y zonificación de áreas potenciales para
el cultivo del cacao en Colombia: dirección de cadenas productivas cadena de cacao - chocolate.
In FEDECACAO. Available in: http://hdl.handle.net/20.500.2324/1907. Last accessed October of
2022.
Gattward, J.N.; A.A.F. Almeida; J.O. Souza; F.P. Gomes & H.J. Kronzucker (2012). Sodium-
potassium synergism in Theobroma cacao: Stimulation of photosynthesis; water-use efficiencyand
mineral nutrition. Physiologia Plantarum 146(3): 350-362.
Guiltinan, M.; G. Lockwood; S. Maximova; A.R. Sena Gomes; G.A. Sodre; B. Laliberte; M. End
& S. Weise (2015). Supplying new cocoa (Theobroma cacao L.) planting material to farmers: A
review of cacao propagation methodologies. Bioversity International. B. Laliberté &
M. End. Rome, Italy. pp. 67-87.
Hartemink, A.E. (2005). Nutrient stocks, nutrient cycling, and soil changes in cocoa ecosystems:A
review. In Advances in Agronomy 86: 227-253.
IDEAM (2019). Irradiación global horizontal media diaria. Interactivo - Radiación Website. Available
in: http://atlas.ideam.gov.co/visorAtlasRadiacion.html. Last accessed July of 2022.
INIAP (2016). Muestreo foliar en cacao. Plegable no. 429. Available in:
http://repositorio.iniap.gob.ec/handle/41000/3881. Last accessed July of 2022.
Isaac, M.E.; F. Ulzen-Appiah; V.R. Timmer & S.J. Quashie-Sam (2007). Early growth and
nutritional response to resource competition in cocoa-shade intercropped systems. Plant and Soil
298(1-2): 243-254.
Lachenaud, P. (1995). Variations in the number of beans per pod in Theobroma cacao L. in the Ivory
Coast. III. Nutritional factors; cropping effects and the role of boron. Journal of Horticultural Science
18
Rev. Fac. Agron. La Plata (2022) Vol 121 (Núm. Esp. 2) Biotecnologías Aplicadas a Cultivos de
Interés Socioeconómico: 1-21
70(1): 7-13.
Leiva-Rojas, E. & R. Ramírez-Pisco (2017). Acumulación y extracción de nutrientes en el cultivo del
cacao (Theobroma cacao L.). International Symposium on Cocoa Research (ISCR). November:
1-7.
León-Moreno, C.E.; J. Rojas-Molina & C.E. Castilla-Campos (2019). Physicochemical
characteristics of cacao (Theobroma cacao L.) soils in Colombia: Are they adequate to improve
productivity? Agronomía Colombiana 37(1): 28-38.
Li, Y.; M. Elson; D. Zhang; Z. He; R. Sicher & V. Baligar (2015). Macro and micronutrient uptake
parameters and use efficiency in cacao genotypes as influenced by levels of soil applied K.
International Journal of Plant & Soil Science 7(2): 80-90.
López, N.R.E. & S.V.A Lucas (2018). Efecto de la fertilizacion con N, S, micronutrientes y
fitorreguladores sobre el rendimiento y rentabilidad del cacao Nacional en el valle del Rio Carrizal.
Ing. Thesis. Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López. Calceta,
Ecuador. pp. 38-54.
Ludeña-Davila, V. (2013). Efecto de la fertilización orgánica y microelementos en el rendimiento de
cacao CCN51 (Theobroma cacao L.) en Jaen. Ingeniería en Conservación de Suelos y Agua. Ing.
Thesis. Facultad de recursos naturales renovables. Universidad Nacional Agraria De La Selva.
Tingo María, Perú. pp. 26-40.
Maathuis, F.J.M. & E. Diatloff (2013). Roles and Functions of Plant Mineral Nutrients. In: Plant
Mineral Nutrients: Methods and Protocols, Methods in Molecular Biology. Frans J.M. Maathuis, Ed.
Humana Totowa, NJ. pp. 1-21.
MADR (2021). Cadena de valor de Cacao. Available in:
https://sioc.minagricultura.gov.co/Cacao/Documentos/2021-03- 31%20Cifras%20Sectoriales.pdf.
Last accessed June of 2022.
Malavolta, E.; G.C. Vitti & S.A. De Oliveira (1997). Avaliação do estado nutricional das plantas.
Principios e aplicaçõe. In: Associação Brasileira para Pesquisa da Potassa e do Fosfato (2a
edicica). Piracicaba.
Mejia Flórez, L.A. & G. Palencia (2000). Efecto de la nutrición en el cultivo de cacao (Theobroma cacao
L.) en la zona productora de Santandar. Seminario Técnico Regional 5(5): 30-33.
Mortimer, R.; S. Saj & C. David (2018). Supporting and regulating ecosystem services in cacao
agroforestry systems. Agroforestry Systems 92(6): 1639-1657.
Motsara, M.R. & R.N. Roy (2008). Guide to laboratory establishment for plant nutrient analysis. Food
And Agriculture Organization of The United Nations Rome, 2008. In Fao Fertilizer and Plant
Nutrition Bulletin 19.
N’Guessan, K.; M.J. Traore; D. Snoeck; E. Kassin; L. Koko; M. Camara & A. Yao-kouame
(2017). Mapping Cacao Fertiliser Requirements in Côte d’Ivoire. Imperial Journal of
Interdisciplinary Research 3(6): 504-515.
Nygren, P. & H.A. Leblanc (2015). Dinitrogen fixation by legume shade trees and direct transferof
fixed N to associated cacao in a tropical agroforestry system. Tree Physiology 35(2): 134- 147.
Oberthür, T.; M. Samson; N.Janetski; K. Janetski & M. Fisher (2018). Response of cacao
seedlings to fertilizer. Better Crops with Plant Food 102(4): 20-23.
Ofori, A.; F.K Padi; A. Akpertey; P. Adu-Gyamfi; M.A Dadzie & F.M Amoah (2017). Variabilityof
survival and yield traits in cacao (Theobroma cacao L.) clones under marginal field conditions in
Ghana. Journal of Crop Improvement 31(6): 847-861.
Osorio, W (2012). El análisis foliar: una poderosa herramienta para diagnosticar el estado nutricional
de los cultivos; pasturas y plantaciones. Manejo Integral del Suelo y Nutrición Vegetal 1(3): 1-4.
Palencia, G.E.; R. Gómez & O. Gûiza (2009). Nuevas tecnologías para instalar viveros y producir clones
de cacao (Theobroma cacao L). Corpoica. Available in:
http://hdl.handle.net/20.500.12324/2194. Last accessed August of 2022.
Puentes-Páramo, Y; J. Menjivar-Flores; A. Gómez-Carabalí & F. Aranzazu-Hernández (2014a).
Absorción y distribución de nutrientes en clones de cacao y sus efectos en el rendimiento. Acta
Agronomica 63(2): 145-152.
Puentes-Páramo, Y; J. Menjivar-Flores; F. Aranzazu-Hernández (2014b). Eficiencias en el uso
de nitrógeno, fósforo y potasio. Bisagro 26(2): 99-106.
Puentes-Paramo, Y.J.P.; A.G. Carabalí & J.C.M. Flores (2016a). Influence of the relationship
19
Carmona-Rojas et al. (2022) Nutrition in cacao crops
among nutrients on yield of cocoa (Theobroma cacao L.) clones. Acta Agronomica 65(2): 176-182.
Puentes-Páramo, Y; J. Menjivar-Flores; F. Aranzazu-Hernández (2016b). Concentración de
nutrientes en hojas, una herramienta para el diagnóstico nutricional en cacao. Agronomía
Mesoamericana 27(2): 329-336.
Quek, R.Y.C.; E.W.Y. Peh & C.J. Henry (2020). Effects of cocoa butter and cocoa butter equivalent
in a chocolate confectionery on human blood triglycerides, glucose, and insulin. Foods 9(4): 455.
Reich, M.; T. Aghajanzadeh & L.J. De Kok (2014). Physiological basis of plant nutrient use
efficiency-concepts, opportunities and challenges for its improvement. Hawkesford, M., Kopriva,
S., De Kok, L., Eds. In: Nutrient Use Efficiency in Plants. Plant Ecophysiology, vol 10. Springer,
Cham. pp. 1-28.
Ribeiro, M.A.Q.; J.O. Da Silva; W.M Aitken; R.C.R. Machado & V.C. Baligar (2008). Nitrogenuse
efficiency in cacao genotypes. Journal of Plant Nutrition 31(2): 239-249.
Romero; F.C. (2018). Biomass and nutrient distribution in cacao trees (Theobroma cacao): A case
study in Cote d’Ivoire. M.Sc. Thesis. Wageningen University. Wageningen, Países Bajos.pp.22-38.
Available in: https://edepot.wur.nl/501084. Last accessed August of 2022.
Rosas-Patiño, G.; Y.J. Puentes-Páramo & J.C. Menjivar-Flores (2017). Relación entre el pH yla
disponibilidad de nutrientes para cacao en un entisol de la Amazonia colombiana. CorpoicaCiencia
y Tecnologia Agropecuaria18(3): 529-541.
Rosas-Patiño, G.; Y.J. Puentes-Páramo & J.C. Menjivar-Flores (2019). Efecto del encalado en el
uso eficiente de macronutrientes para cacao (Theobroma cacao L.) en la Amazonia colombiana.
Ciencia y Tecnología Agropecuaria 20(1): 5-16.
Ruales-Mora, J.L.; H. Burbano-Orhuela & W.P. Ballesteros (2011). Efecto de la fertilización con
diversas fuentes sobre el rendimiento de cacao (Theobroma cacao L.). Revista de Ciencias Agrícolas
28(2): 81-95.
Salvador, N.; E. Espinosa & J.C. Rojas (2012). Manual del cultivo de cacao blanco de Piura. Mesa
Técnica Regional de Cacao de Piura. Dirección Regional Agraria Piura; PER. Available in:
https://doi.org/http://www.swisscontact.org.pe/ biblioteca-virtual. Last accessed August of 2022.
Sánchez, L.E.; D. Parra; E. Gamboa; & J. Rincón (2005). Rendimiento de una plantación comercial
de cacao ante diferentes dosis de fertilización con NPK en el sureste del estado Táchira,
Venezuela. Bioagro 17(2): 119-122.
Sánchez, V.; J.L. Zambrano; C. Iglesias; E. Rodríguez; V. Villalobos & F.J. Díaz (2019). La
Cadena de Valor del Cacao en América Latina y El Caribe. In La cadena de valor del cacao en
América Latina y el Caribe. Available in: http://repositorio.iniap.gob.ec/handle/41000/5382. Last
accessed August of 2022.
Santana, M.B.M. & P. Cabala-Rosand (1982). Dynamics of Nitrogen in a Shaded CacaoPlantation.
Plant and Soil 67(1/3): 217-281.
Shin, R. (2014). Strategies for improving potassium use efficiency in plants. Molecules and Cells
37(8): 575-584.
Silva, S. de A.; J.S. Lima de S; G.S. Souza; R.B. Oliveira & A.C. Xavier (2010). Lógica fuzzy na
avaliação da fertilidade do solo e produtividade do café conilon. Revista Ciencia Agronomica 41(1): 9-
17.
Snoeck, D; M.K. Abekoe; A.A Afrifa & M.R. Appiah (2007). The soil diagnostic method to compute
fertilizer requirements in cocoa plantations. International Conference on Soil Science Accra;
January; 7–11. Available in: https://agritrop.cirad.fr/554340/1/document_554340.pdf. Last
accessed October of 2022.
Snoeck, D.; A.A. Afrifa; K. Ofori-Frimpong; E. Boateng & M.K. Abekoe (2010). Mapping fertilizer
recommendations for cocoa Production in Ghana using soil diagnostic and GIS tools. West African
Journal of Applied Ecology 17(January 2014): 97-107.
Snoeck, D; L. Koko; J. Joffre; P. Bastide & P. Jagoret (2016). Cacao Nutrition and Fertilization.
E. Lichtfouse, Eds. In: Sustainable Agriculture Reviews. Sustainable Agriculture Reviews, vol
19. Springer, Cham. 155-202 pp.
Sodre, G.A.; P.C.L. Marrocos; R.E. Chepote & R.G. Pacheco (2001). Uso do desvio padrão para
estimativa do tamaño de amostra de plantas de cacau (Theobroma cacao L.) em estudos de
nutriçao. Agrotropica 145-150.
Sodré, G.A.; P.C.L. Marrocos & R.G. Pacheco (2010). Quality of cocoa plants produced by stem cuttings
20
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