A

qdlolave
Glass unthlat tess stout

Atmperotre
nelp
Mhntrals, vlann5 can

Alfzator of ofceps oone for inshyrnons 1htotgtisr

Done UMder

calttd plhanol
*4 for wtptna hands,
butae cletlzahon or chental
eyiliakon l

kmin

be 44ollued
44fs cdot oInoola tirng 4cchlqvcs
 ConinoUS Stmt
uhe 6\ahc Susptnsttulhre
omn
iedium of ype neUpon Dependiny
:hat cultore
foy dvepart
the Upon
(ulhres Types
alals
tadesJondituro
a cauws
Conditions. skuentatté (xplant
Orgar odes felds Dlesorstt
akoo
explan: developmen ges to
plant stage4he Hosdening
nt 5ndlyect addedshoo+ kirjntor Cyto
mdio new rnedi
b a old
cuikring bub
mcdes?
al
agqent6ohdikComes
jing
vsedA sutyo5e
15 SouTce
) bon Can
Cine, MÍovo pE)amountnobienk
(W, Aotge thero tients
j tnosganic
nu 4
s ntcalty Pants bseclsythes i nyients
) Drgori w
of
media componeots Major
 mdiun >Asepte
rial mate plnutrieq
at catiprcpat
of on Mepte
are, cultur e calle6 for teria cri thYCe aie
ciCvlhre
anqes3wovpiecalloyqis cal of
Predve
outs. an oots incur
OccuY
stge Msrphogene
sis s
division
cell Rapid j
stage aie protifer B>
ivik. tobegi
tn
begln dediffetHha
and te xplant fncecle >
1HdveAoM
culte callys Btages 1kree
Popetes.
eilsei
th
singe
siogk isclate
isclate tUed (an -)4
ie cet stem
dat. qioco5
n and ssthe tosgn PHo No- i
20 ospant Rom mecha folid
ofnCISS tus-a a )
on
g1tn plant
cetis Cndifferentiatd
ebsBats.
ype
tupe
losed e
 (atkus uhafa fon
Sub- u tore
catlus be intiated ovn (ulwre meda
e Acively qrooin9 (an

with an eYM Pnysiolegi ca l balane cfof cytozinlo

biomass

e 44e whole plqnt Can

calos th'ssve ugh manipulafon of fe
nubtent cdnd harmDnal ta
is
SoUr(e f Cultre medtug
cel gent tc vavigbily
suspension cultie in moving itquid me divm caqn b
inha te d Kom Cal0s. culhe2
vay vsef o obtan Co
Seto nday etabolites. mmericaly (mpo ran
tseveral iochemical assay Can
be pcotomed from Cllus
cuture

Gsprson ruttne

(o ntaln gar but contas atl tind als in ib.

tultre
11 meuns K cultie cultre mediun.
in a fized volune of
gtneral asHukicnt mecdium Cnd celular tnowkum are
mied, aerated and allowedto grow
Batik culhe iLa medm is depletal
the
qroo
of wtr (pn ts and mefu bo lit foyproducs
 contincus chornges the mecdium conn positisn
chonges in te
ContinoUs intenal e hange's in ccilular a ctomo laton of
meBabolfc
oolf c pro du cts.
(t ctllk Sn tule chi bl the Ao tto tol ng p
qroutn yele
) dag phae where cel(s pree ave to, diulciuy at
ce l division is 1
0J xponen hal phase -whexe the xate of
3) lne aY phae;- ce ll divtsin slo ws but rate of cell (rpan slon
A) Decele rahon phse i cel( divsjon and elongation decces
S) stai onary phase : af cet ls venain constan.
no
and siz
bemnfcontinoo5 suspension culbv
reribdieYemoval
(ontino5 sUs Pensi on culkre, toso
* Gmpositon of medta ls no
change,, ohanish
they ase
Continou5. cnarg:
muluhd n taere.

Bosed o4
Chemostat t mywen chemical
tgt to. chongc
-he meclicu
Tur bid oslst
hclp in chang fmeclia,
 1ke vst meda is elaed with fesh
vnedta, hene 4he ceus rom Ued medivm ne
-chani cuy
as
gon colbre
CMertster root ip cultuse)gp
It, shoot ttp cote c Meriskm -shoot ip
coltureo)
Atak prlmo1 dta totture 3
iy flowIY Culhre ( me ris ten floral colhey
V Antrer and poler cuthys

-4ppltatios od Biolechnalogy akout (otimal lelant) ietnations

of biotehnology and plant biotechnologu i)
Biotchnoloq4 defnalio4
eioeck oloy -echudlogy Ahal uklaes iologica f aaems.
bigorisms devclop
producs.
Bieuing and koking bred
Plut eilehndlogy dekaaton
Plants for
4ftuatous 4hat 6r opporkanites.
Conbine my thple needs and ppurknites
aYe CommoM.