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    Cell Respiration

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    yeshuammu09

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    Cell Respiration

    Uploaded by

    yeshuammu09
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    7 views2 pages

    Cell Respiration

    Uploaded by

    yeshuammu09

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Cell respiration

    Do soaked beans respire more than dry beans?


    Cellular respiration occurs in plant cells as well as animal cells. In this activity, you will measure oxygen
    consumption by germinating seeds. You need to know that most seeds dry out and are in a dormant
    condition before germination. In this state, their metabolism is extremely slow. When sufficient moisture is
    present in the environment, seeds absorb water and swell. This triggers enzyme activity. All seeds store
    nutrients, usually starch, and the appropriate enzymes digest it to release glucose as an energy source. With a
    supply of energy, cellular respiration can occur.
    Equation For Cellular Respiration

    C6H12O6 + 6O2 -> 6CO2 + 6H2O + energy

    Carbon dioxide is formed as oxygen is used. The pressure due to C02 might cancel out any change due to the
    consumption of oxygen. To get rid of this problem, a chemical will be added that will selectively take out C02.
    Potassium hydroxide will chemically react with carbon dioxide by the following equation:

    C02 + 2 KOH -> K2CO3+ H20

    A respirometer is the system used to measure cellular respiration. Pressure changes in the
    respirometer are directly relative to a change in the amount of gas in the respirometer, as long as the
    volume and the temperature of the respirometer do not change.

    Procedure;

    What is your hypothesis?

    Obtain 6 50ml conical tubes, 2 glass culture


    bowls, absorbent cotton and non-absorbent
    cotton.

    In all 6 tubes add a small amount of absorbent


    cotton (so that it fills the “conical” portion of
    the tube).

    To all 6 tubes add 5 drops of 15% KOH to each


    cotton ball. Add a small “wad” of
    nonabsorbent cotton (to keep the beans away
    from the KOH solution)

    To 2 of the tubes add soaked beans up to the


    20 ml level and cover with nonabsorbent cotton. You will be inverting the tubes so be sure you have
    enough cotton to keep the beans from falling out.
    To 2 of the tubes add dry beans up to the same level as the soaked beans. Again, add nonabsorbent
    cotton, since you will be inverting the tubes.

    Finally to the last 2 tubes, add rocks up to the level of the soaked beans, add the nonabsorbent cotton
    and make sure none of the contents falls out when inverted.

    Create two sets; 1 soaked, 1 dry and 1 rocks and rubber-band them together. Invert the sets into the
    glass culture bowls. Add water until the bowl is half full, add 1 drop of food coloring. Leave one set at
    room temperature and place the other in the incubator. Let the set up respire for a day or more.

    Graph the change in level of the water in the 6 tubes

    Did you support or refute your hypotheses?

    Which set of beans respired the most?

    How can you detect that more respiration has occurred?

    How does the rate of oxygen usage differ between the two conditions? Which set respired the most? Why?

    Why do I have you include a tube full of rocks?

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