Primovert

Primovert
Operating Manual
ZEISS Copyright Primovert
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to instructions
concerning the safe handling of the instrument.
Subject to alterations in the interest of technical progress. The manual is not covered by an update
service.
© Unless expressly authorized, forwarding and duplication of this document, as well as utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10
07745 Jena, Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH

Königsallee 9-21
37081 Göttingen, Germany
Manual number: 415510-7244-001

Date of issue: Version 12, 12/10/2014
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Primovert Contents ZEISS
CONTENTS
Page
1 Notes on Instrument Safety ............................................................................................ 5
1.1 General Safety Notes .......................................................................................................... 5
1.2 Instrument Safety and EMC................................................................................................. 5
1.3 Unpacking, Setup, Transportation, Storage .......................................................................... 6
1.4 Disposal.............................................................................................................................. 6
1.5 Operation ........................................................................................................................... 6
1.6 Warranty Notes .................................................................................................................. 8
1.7 Warning and Information Labels.......................................................................................... 9
2 Description ..................................................................................................................... 10
2.1 System Overview .............................................................................................................. 10
2.2 Intended Use .................................................................................................................... 12
2.3 Instrument Description and Main Features ......................................................................... 12
2.4 Objectives......................................................................................................................... 14
3 Start-Up and Operation ................................................................................................. 15
3.1 Starting up the Microscope ............................................................................................... 15
3.1.1 Setting up the Microscope ................................................................................................ 15
3.1.2 Screwing in the Objectives ................................................................................................ 15
3.1.3 Inserting the Condenser .................................................................................................... 16
3.1.4 Inserting the Filter Slider .................................................................................................... 16
3.1.5 Inserting the Phase Contrast Slider .................................................................................... 17
3.1.6 Inserting the Stage Inserts and Attaching the Stage Enlargement Plates .............................. 17
3.1.7 Mounting the Specimen Guide and Inserting the Mounting Frame ..................................... 18
3.1.8 Mounting UV Protection Plate onto Primovert iLED ............................................................ 19
3.1.9 Transmitted-Light Illuminator: Inserting the LED ................................................................. 20
3.1.10 Connecting the Stand with Binocular Tube or Phototube to the Power Supply System ........ 20
3.2 Operating the Microscope ................................................................................................. 21
3.2.1 Operational and Functional Elements of the Primovert Microscope ..................................... 21
3.3 Primovert HDcam .............................................................................................................. 27
3.3.1 Control and Functional Elements of the Integrated Camera ................................................ 27
3.3.2 Connect iPad to USB charge port ...................................................................................... 29
3.3.3 Ports and their Possible Uses ............................................................................................. 30
3.3.4 Remote Control of Integrated Camera .............................................................................. 34
3.3.5 Configuring the Integrated HD IP Camera.......................................................................... 35
3.4 Switching the Microscope On / Off .................................................................................... 39
3.5 Adjusting Interpupillary Distance and Viewing Height......................................................... 40
3.6 Compensating Defective Vision at the Eyepieces and Inserting the Eyepiece Pointer or
Eyepiece Micrometer......................................................................................................... 41
3.7 Adjusting Transmitted-Light Phase Contrast ....................................................................... 42
3.8 Reflected-Light Fluorescence ............................................................................................. 43
3.9 Retrofitting the Microscope ............................................................................................... 43
3.9.1 Replacing the Halogen Bulb 6 V / 30 W of the Transmitted-Light Illuminator or the
Transmitted-Light Illuminator with LED .............................................................................. 43
12/2014 415510-7244-001 3
ZEISS Copyright Primovert
3.9.2 Attaching a Camera to the Stand with Binocular Phototube ............................................... 44

3.10 Care, Maintenance and Troubleshooting ........................................................................... 46
3.11 Instrument Care ................................................................................................................ 46
3.12 Troubleshooting on the Microcope .................................................................................... 47
3.13 Troubleshooting on the Integrated HD IP Camera .............................................................. 49
4 Technical Data ................................................................................................................ 51
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Primovert Notes on Instrument Safety ZEISS
1 NOTES ON INSTRUMENT SAFETY
1.1 General Safety Notes
Please read this operating manual carefully before starting up the microscope.
If you need supplementary information, contact the Carl Zeiss Service or an authorized agency.
To ensure safe operation and troublefree function of the microscope, strictly observe the precautions and
warnings given in this manual.
They are set off herein as follows:
CAUTION
Hazard to the user of the instrument if safety notes are not observed.
ATTENTION
Hot surface!
ATTENTION
LED risk group 2 according to IEC 62471, LED radiation is emitted.
Do not look into the LED beam. It can be dangerous to the eyes.
ATTENTION
Disconnect the plug-in power unit from line power before opening the microscope!
ATTENTION
This symbol indicates a possible hazard to the instrument or system.
NOTE
Working instructions to be observed when using the microscope.
1.2 Instrument Safety and EMC
The Primovert microscopes have been designed, produced and tested in compliance with the standards
DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 "Safety requirements for electrical equipment for
measurement, control and laboratory use".
The Primovert microscopes meet the requirements of the EC Directive 98/79/EC Annex 1, RoHS Directive
2011/65/EC and carry the mark.
The instruments have to be disposed of in compliance with the WEEE Directive 2011/65/EU and
RoHS Directive 2012/19/EU.
12/2014 415510-7244-001 5
ZEISS Notes on Instrument Safety Primovert
1.3 Unpacking, Setup, Transportation, Storage
Please observe the following safety notes for unpacking, transportation and storage of the microscope:
− The microscope is supplied packed to commercial standards in a plastic case with outer cardboard
package; use the original packaging for transportation.
− Retain the original packaging for a longer storage of the microscope or its return to the manufacturer.
− When unpacking the equipment, check all components for completeness according to the delivery
note.
− Keep transport and storage temperatures as specified in the Technical Data.

− Set up the microscope on a stable worktable with solid and smooth tabletop.
While using the microscope and up to about 10 minutes after the use there is risk of burns due
to the hot lamp housing.
1.4 Disposal
Please observe the following safety notes for disposing of the microscope:
Defective microscopes should not be disposed of with household waste; dispose of them in
compliance with the provisions of the law.
The manufacturer of the device is under the legal obligation to take back defective devices.
1.5 Operation
The microscope including its original accessories must not be used for microscopic techniques other than
those described in this operating manual.
Please observe the following safety notes when using the microscope:
The manufacturer cannot assume any liability for other applications, including those of
individual modules or single components. This also applies to any service or repair work that is
not carried out by authorized service personnel. In case of non-compliance, all warranty claims
shall be forfeited.
Do not operate the devices and their accessories included in the delivery in potentially explosive
areas nor in the presence of volatile anesthetics or combustible solvents, such as alcohol,
benzine or similar chemicals.
Dirt and dust may impair the performance of the devices. The devices must therefore be
protected from such influences to the greatest possible extent and covered with the dust cover
when not in use. Before covering the devices always check whether they have been switched
off or have switched off themselves automatically in AUTO-OFF mode (then, the blue power-
on LEDs on the right and left side of the stand are off).
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The microscope may only be operated by trained personnel who are aware of possible dangers
involved in microscopy and the particular application concerned. The microscope may only be
operated if set up on a stable, solid, smooth and hardly flammable surface.
The microscope is a high-precision instrument that may be impaired in its performance or even
destroyed when handled improperly.
The microscope is equipped with a plug-in power unit allowing line voltages in the range
between 100 and 240 V ±10%, 50 / 60 Hz, without the need for changing the voltage setting
on the instrument.
The plug-in power unit meets the requirements of protection class II (with protective
insulation). If its casing is damaged, put the plug-in power unit out of operation. The
microscope may be operated only with the plug-in power unit supplied.
If any protective measures are no longer effective, the device must be taken out of service and
secured against inadvertent operation. Please contact a Zeiss service agency or the
Carl Zeiss Microscopy Service to have the device repaired.
− Always disconnect the power cable before opening the instrument and changing the bulb
or LED.
− Wait for the bulb to cool down before replacing it and do not leave fingerprints on the new
bulb.
− The instrument may only be opened by instructed specialists or service staff.
− The operation of the instrument in explosion-risk environments is not allowed.
Do not replace detachable power cables by power cables with inadequate specifications. Only
the specified power cables should be used.
The microscope can only safety disconnect from line power, when the plug-in power unit is
disconnected from line power. The rotary knob for switching the microscope off (Fig. 11/19)
switchs only to stand by.
Never look into the light beam - neither with nor without optical instruments, even not if you
simply want to observe the specimen. Your eyes may be damaged in case of non-observance!
When using immersion oil, read in any case the safety data sheet.
Immersion oil irritates the skin. Avoid any contact with skin, eyes and clothing.
After skin contact, wash the oil off with plenty of water and soap.
After eye contact, immediately rinse the eye with plenty of water for at least five minutes. If
the irritation persists, consult a medical specialist.
Proper disposal of immersion oil: Take care to ensure that immersion oil does not enter surface
water or the sewage system.
12/2014 415510-7244-001 7
ZEISS Notes on Instrument Safety Primovert
The microscope is not equipped with special devices for the protection from corrosive,
potentially infectious, toxic, and radioactive or other substances that may be hazardous to
health. If you handle such substances, be sure to observe all legal requirements, in particular
the relevant national accident prevention regulations.
− Before transporting the instrument, switch it off and let it cool down (hot surface on the
lamp housing).
− The plug-in power unit must not get in contact with moisture.
Obstruction or covering of ventilation slits may result in heat build-up which may damage the
instrument and in extreme cases cause fire. Always keep the ventilation slits clear and ensure
that no objects enter the instrument through the ventilation slits.
1.6 Warranty Notes
The Primovert microscopes including their original accessories must not be used for microscopic
techniques other than those described in this operating manual. The manufacturer cannot assume any
liability for other applications.
Please consider the following warranty notes for the microscopes:

− The manufacturer guarantees that the device is free from material or manufacturing defects when
delivered.
− Any defects must be notified to us immediately and steps be taken to minimize damage.
− If notified of such a defect, the manufacturer is obligated to rectify it at its discretion, either by
repairing the instrument or by delivering an intact replacement.
− No guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper
use.
− The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or
any other tampering with the microscope, particularly the removal or replacement of microscope
components, or the use of accessories from other manufacturers.
Unauthorized tampering with the instrument shall lead to a forfeit of all warranty claims.
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1.7 Warning and Information Labels
Fig. 1 Warning and information labels on the Primovert
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ZEISS Description Primovert
2 DESCRIPTION
2.1 System Overview
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Primovert Description ZEISS
12/2014 415510-7244-001 11
2.2 Intended Use
The Primovert microscopes are universally applicable light microscopes of inverted design (inverted
microscopes). They are primarily used to examine cell and tissue cultures as well as sediments in culture
flasks, Petri dishes and microtiter plates.
Typical applications (examples):
Observation of intracellular processes on living cell cultures, cell-cell interactions, motility, growth,
examination of blood and tissue samples from the human body.
When handling hazardous substances, observe the instructions on intended operation, correct use and
statutory safety precautions.
2.3 Instrument Description and Main Features
The Primovert microscopes are inverted transmitted-light microscopes of compact design with a small
footprint.
The user can choose from among five types of stands for the brightfield and phase contrast in
transmitted light microscopic techniques.
− Stand-type Primovert with binocular tube
− Stand-type Primovert Photo with binocular phototube for photo and video documentation
− Stand-type Primovert Ergo with binocular ergotube, continuous angle adjustment from 30° to 60°
− Stand-type Primovert HDcam with integrated HD IP CMOS camera and various interfaces and setting
options
− Stand-type Primovert iLED with integrated LED-fluorescence unit (one channel) and binocular
phototube
Important features of the microscope are:
− Stand equipped with a binocular tube or a binocular phototube (50 % vis, 50 % doc) with an
ergonomically favorable tube angle of 45°. The binocular part can be tilted upward and downward
and adapted to the individual eye distance. As there is an upper and lower binocular part position, the
user can choose between two viewing heights.
− Stand equipped with an ergotube with variable tube angle, continuously tiltable in the range from
30° to 60°; the binocular part can be tilted upward and downward and adapted to the individual eye
distance.
− Illumination either by a 6 V / 30 W halogen lamp insert or an LED.
− Continuously adjustable illumination intensity.
− Blue light-intensity indicators installed on both sides, which are visible from a distance.
− External plug-in power unit with cable (incl. cable with multiple plug and country-specific plug inserts).
− Plastic-coated carrying handle integrated in the stand for setting up, demounting and transporting the
device.
− Convenient coaxial coarse and fine focusing drive; adjustable torque of coarse focusing drive.
− Fixed specimen stage suitable for inserting metal or glass plates and attaching the stage enlargement
plates and the specimen guide.
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Primovert Description ZEISS
− Quadruple nosepiece with W 0.8" lens thread running on ball bearing.

− Infinity-corrected "Plan-ACHROMAT" objectives with magnifications of 4x and 10x for brightfield and
phase contrast, as well as LD "Plan-ACHROMAT" objectives with long working distance and
magnifications of 20x and 40x for brightfield and phase contrast.
− Preadjusted phase contrast: Use of only one phase stop for objectives with magnifications of 10x, 20x
and 40x for phase contrast.
− Adjustable eyepieces 10x for field-of-view number 20, suitable for spectacle wearers.
12/2014 415510-7244-001 13
2.4 Objectives
The objectives are the optical heart of the microscope. The objectives may be labeled as follows:
LD Plan-ACHROMAT 40x/0.5 Ph 1 ∞/1.0
Where:
LD Long working distance
40x Objective magnification,
with a defined color ring on the
objective being assigned to each
magnification step (Carl Zeiss color code)
0.5 Numerical aperture
∞ Infinite mechanical tube length
1.0 Usable with cover glass thickness
D = 1.0 mm, similar data for other glass
cover thicknesses in millimeters
or
0 Usable without cover glass
Fig. 2 Objective (mounting position)
− Usable with cover glass thickness D = 0 or
0.17 mm
Other labels:
Ph Phase contrast objective with green
inscription
The total visual magnification of the stand with tube / phototube is obtained by multiplying objective
magnification by eyepiece magnification, e.g., 40 x 10 = 400x.
Numerical aperture multiplied by 1000, e.g. 0.5 x 1000 = 500x, presents the maximum useful
magnification; there is no resolution for further details above that limit.
If glass cover slips are employed, objectives can also be used with other cover glass thicknesses.
Available objectives:
Objective Magnification NA Description Working Order No. Field of
distance view
(mm) (mm)
Plan-Achromat 4x 0.10 HF 12 415500-1600-001 20
Plan-Achromat 4x 0.10 Ph0 12 415500-1619-000 20
Plan-Achromat 10x 0.25 Ph1 4.4 415500-1605-001 20
LD Plan-Achromat 20x 0.30 Ph1 4.6 415500-1614-000 20
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Primovert Start-Up and Operation ZEISS
3 START-UP AND OPERATION
3.1 Starting up the Microscope
3.1.1 Setting up the Microscope
Before installing and starting up the microscope, be sure to carefully read and subsequently
observe the notes on instrument safety (see Section 1).
The microscope is supplied completely assembled and, inclusive of its accessories, packed to commercial
standards.
The accessories and individual microscope components (such as sliders, filters or phase stops, specimen
holders, specimen guides or the stage enlargement plates) are delivered in separate packages and must
still be mounted to the microscope.
• Take the microscope out of the transport case and put it on the worktable, using the handle on the
back of the stand. Do not hold the microscope on the front side by the eyepiece part, but by the neck
of the stand between the eyepiece part and the stand base or at the front and at the back of the
stand base.
Retain the original packaging for a longer storage of the microscope or its return to the
manufacturer.
3.1.2 Screwing in the Objectives
• Remove the dust caps from the location holes

of the nosepiece (Fig. 3/2).
• Screw the objectives (Fig. 3/1) in ascending
order into the location holes until the stop is
reached.
• Affix the supplied stickers (Fig. 3/3) indicating
the objective magnification, to the
corresponding positions of the nosepiece.
Recommendation:
Affix the stickers so that you can read
from the left side (Fig. 3/3) the
magnification of the objective just
positioned in the light path.
Fig. 3 Screwing in the objectives
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ZEISS Start-Up and Operation Primovert
3.1.3 Inserting the Condenser
• Remove the condenser cap.

• Insert the condenser (Fig. 4/4) from the front
into the guide (Fig. 4/3) and push it in to stop.
• Lock the condenser by tightening the socket
head cap screw or the knurled thumb screw
(Fig. 4/1), which is equally supplied with the
microscope.
Recommendation:
If condensers are frequently exchanged
or whenever larger glass vessels are to
be used frequently without a
condenser, the knurled thumb screw
Fig. 4 Inserting the condenser supplied rather than the socket head
cap screw (Fig. 4/2) should be
employed to lock the condenser.
3.1.4 Inserting the Filter Slider
• Put the color filter (Fig. 5/1) or the green

interference filter (diameter d=45 mm) into the
opening of the filter slider.
As the filters are clamped in the filter
slider only slightly, not twist-proof, the
filter slider should not be turned or
tilted in order to avoid that the filters
fall out and get damaged.
• Push the 2-position filter slider (Fig. 5/2) from
the left or the right side into the slot (Fig. 5/3)
of the upper part of the stand until it engages
in the desired position.
When using the Primovert iLED in

fluorescent mode, use the yellow filter
Fig. 5 Inserting the filter slider inserted into the filter slider (included
in the delivery). It prevents scattered
fluorescent background generated by
unwanted transmitted LED source
phosphor.
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3.1.5 Inserting the Phase Contrast Slider
The 2-position Ph slider (Fig. 6/1) and the

3-position Ph slider (Fig. 6/2) are delivered
completely, with phase contrast stops mounted.
• Push the Ph slider (Fig. 6/1 or 2) from the right
or the left side, with the inscription facing
upwards, into the condenser slot (Fig. 6/3) until
it engages in the desired position.
If necessary, the neutral filter can be

inserted into the free position of the
corresponding phase contrast slider.
This prevents the user from being
dazzled when the slider position is
changed.
Fig. 6 Inserting the phase contrast slider
3.1.6 Inserting the Stage Inserts and

Attaching the Stage Enlargement
Plates
• Insert either the metal insert (Fig. 7/5) or the

glass insert (Fig. 7/6) into the specimen stage.
The metal insert with a bean-like opening is to be
used to prevent an objective with short working
distance (e.g. 40x) from colliding with the sample
container when being rotated into the light path.
Because of the longer working distance, LD
objectives (LD = long distance) can also be used.
The stage insert with bean-like opening is provided
with an orientation pin to fix its position.
• If the size of the stage surface does not suffice,
stage enlargement plates (Fig. 7/1 and 3) can be
attached to both the left and the right side. The
stage enlargement plates have to be attached
to the stage (Fig. 7/4) from below and fixed
using the two knurled screws (Fig. 7/2) included Fig. 7 Inserting the stage inserts and
in the delivery. attaching the stage enlargement
plates
If the specimen guide is used, it is not possible to use the stage enlargement plate on the
right side of the stage.
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3.1.7 Mounting the Specimen Guide and

Inserting the Mounting Frame
• If attached, remove the stage enlargement plate

from the right side of the stage.
• Attach the specimen guide (Fig. 8/2) from
below to the right side of the stage (Fig. 8/1)
and fasten it with the two knurled screws
(Fig. 8/3) of the specimen guide.
• Insert the universal mounting frame M or the
mounting frame for 96-position microtiter
plates (Fig. 8/5) into the specimen guide,
depending on the sample vessel used. Make
sure that the mounting frame stops properly in
the two fixing clips (Fig. 8/4).
• For Petri dishes with 65 mm or 54 mm
Fig. 8 Mounting the specimen guide and diameter, insert the corresponding mounting
inserting the mounting frame frame into the mounting frame for microtiter
plates.
If required, the mounting frame for Petri dishes d=35 mm can be inserted additionally into
the mounting frame for Petri dishes d=65 mm.
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3.1.8 Mounting UV Protection Plate onto

Primovert iLED
• Put the UV protection plate (Fig. 9/1) on the

Primovert fluorescence stand.
• Fix it with the screws (Fig. 9/2) on both sides.
1 UV protection plate
2 Screws
Fig. 9 Mounting UV protection plate onto
Primovert iLED
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3.1.9 Transmitted-Light Illuminator: Inserting the LED
The microscope is delivered with the transmitted-light illuminator with halogen bulb 6 V 30 W/LED 3 W
already mounted.
If the transmitted-light illuminator with LED (halogen bulb) is to be additionally employed, it must be
inserted into the stand instead of the transmitted-light illuminator with halogen bulb (LED)
(see Section 3.9.1).
3.1.10 Connecting the Stand with

Binocular Tube or Phototube to the
Power Supply System
• If necessary, replace the power supply adapter

attached to the plug-in power unit (Fig. 10/1)
by one of the country-specific adapters supplied
(Fig. 10/3), pulling off the existing adapter and
inserting the desired one.
• Insert the plug (Fig. 10/4) of the plug-in power
unit into the connection socket located on the
rear of the stand (Fig. 10/5).
• Connect the plug-in power unit (Fig. 10/1) with
a power outlet.
Fig. 10 Connecting the plug-in power unit • If the plug-in power unit cannot be plugged
into the chosen power outlet because of limited
space, replace the power outlet adapter by the
supplied IEC adapter (Fig. 10/2). This allows the
plug-in power unit to be put flat onto the
tabletop and connected to the power outlet
through a country-specific appliance cable.
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3.2 Operating the Microscope
3.2.1 Operational and Functional Elements of the Primovert Microscope
Fig. 11 Operational elements of Primovert with binocular tube, ergotube and phototube
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Legend to Fig. 11:

1 Transmitted-light illuminator (built-in)
2 Filter slider
3 Slider with phase stops
4 Handle (rear)
5 Control knob for Y travel of specimen guide (travel readable on alphabetic scale reflected in the mirror)
6 Control knob for X travel of specimen guide (travel readable on numeric scale)
7 Specimen guide
8 Specimen stage
9 Fine focusing drive (right side)
10 Coarse focusing drive (right side)
11 Rotary knob for adjusting the illumination intensity
12 On/off switch for illumination; active with rotary knob in AUTO position
13 Tube
14 Binocular part of the tube
15 Eyepieces
16 Camera port (only stand with binocular phototube)
17 Lever for adjusting the aperture diaphragm of the condenser
18 Objective
19 Rotary knob for switching the microscope on and off, with ON, OFF and AUTO positions
20 Ring for adjusting the torque of the coarse focusing drive
21 Coarse focusing drive (left side)
22 Fine focusing drive (left side)
23 Nosepiece
24 Illumination intensity indicators (on both sides of the stand)
25 Plug-in power unit
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Fig. 12 Operational elements of Primovert HDcam with integrated camera
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Legend to Fig. 12:

2 Filter slider
4 Handle (rear)
5 Specimen stage
8 Rotary knob for adjusting the illumination intensity
9 USB socket (5 V / 2 A) for iPad charging
11 Control elements
12 Holder for iPad
18 SD card slot
19 Nosepiece
Operating of Primovert HDcam with integrated camera with iPad

• Swivel down the holder (Fig. 13/2) and insert
the iPad (Fig. 13/1).
1 iPad
2 Holder
Fig. 13 Usage of Primovert HDcam with
iPad
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Fig. 14 Operational elements of Primovert iLED with integrated fluorescence unit
12/2014 415510-7244-001 25
Legend to Fig. 14:

2 Filter slider
4 Handle (rear)
5 Specimen stage
6 Rotary knob for adjusting the transmitted light illumination intensity
10 Tube
11 Binocular part of the tube
12 Eyepieces with eyecups
13 Camera port (only stand with binocular phototube)
19 Rotary knob for adjusting the fluorescent illumination intensity
20 Nosepiece
21 Transmitted light / reflected light changeover switch (Brightfield / Fluorescence)
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3.3 Primovert HDcam
3.3.1 Control and Functional Elements of the Integrated Camera
Do not operate the camera in a

potentially explosive environment!
Operation of the camera in a potentially
explosive environment, in the presence
of volatile anesthetics or flammable
solvents such as alcohol, benzene or
similar may result in explosions or
deflagrations.
• Briefly press the snap button (Fig. 15/8) to

trigger an exposure.
• Press the snap button for longer to start a
video recording.
1 LED display
• During a video recording briefly press the snap
2-7 Function buttons, explanation see Table 1
button to stop recording. 8 Snap button
• Press the menu button (Fig. 15/2) to open the 9 Infrared sensor for remote control
On Screen Display (OSD) menu. Fig. 15 Control elements of the integrated
camera
Pos. No. Standard function Function in OSD menu

2 Activate menu Exit menu
3 AWB (trigger automatic white balance) OK (apply value)
4 Increase contrast Up / increase value
5 Increase brightness Right
6 Reduce contrast Down / reduce value
7 Reduce brightness Left
Table 1 Key assignment standard function / function in OSD menu
The visualized images of the integrated camera may only be used for training and research.
Direct generation of diagnostic results from these images is not proposed.
12/2014 415510-7244-001 27
The camera has a number of ports:

− USB 2.0 port (Fig. 16/1) for data communication
when connected to PC, as well as power supply,
see section 3.3.3.2
− HDMI port (Fig. 16/3) for image data transfer to
a monitor, TV or projector, see section 3.3.3.3
− Fast Ethernet port (Fig. 16/2) for communication
and image data transfer, Ethernet port can
connect to external router, see section 3.3.3.4
The microscope stand has a SD / SDHC card port
(Fig. 18/2) at the left side for storing image data,
1 USB 2.0 see section 3.3.3.1.
2 Netport in Primovert HDcam
3 HDMI
Fig. 16 Interfaces on reverse side
The following connecting cables are required for use of the ports:
Item Name Figure Remarks

1 USB 2.0 cable Connection between
integrated camera and PC
2 HDMI cable Connection between

(not supplied) integrated camera and
monitor, TV or projector
3 HDMI / DVI adapter Connection between

cable integrated camera and
(not supplied) monitor or projector
4 Ethernet cable Connection between

integrated camera and
network or WLAN router
Table 2 Connections
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The various states of the integrated camera are displayed by LED.
System status LED display / operating mode

Start sequence LED lights up green
Start sequence completed LED flashes green
Ready for operation LED lights up green
Storage process LED flashes green
Error LED flashed red for approx. 5 seconds
Table 3 LED status displays
3.3.2 Connect iPad to USB charge port
• If you want to load the iPad use the USB charge

port (Fig. 17/3) for iPad.
• Connect the iPad via USB cable with the USB
charge port in front of the iPad holder.
• For this purpose swivel down the holder
(Fig. 17/2) and insert the iPad (Fig. 17/1).
• Insert the mini USB plug into the USB 2.0 port
on the iPad.
• Insert the USB plug into the USB charge port
(Fig. 17/3) in front of iPad holder.
1 iPad
2 Holder
3 USB charge port for iPad
Fig. 17 Connect iPad to USB charge port
12/2014 415510-7244-001 29
3.3.3 Ports and their Possible Uses
3.3.3.1 Saving Data to the SD Card via SD Card Port
An SD card has a max. capacity of 4 GB, an SDHC (HC = high capacity) up to 32 GB. Prior to
use on the PC, the SDHC card must be formatted in the format FAT32.
• Insert an SD / SDHC card (Fig. 18/3) into the

card slot (Fig. 18/2).
• Briefly press the snap button 1 second
(Fig. 18/1) to trigger an exposure.
The images are saved on the
SD / SDHC card in JPG format.
• Press the snap button (Fig. 18/1) for 2 seconds
to start a video recording.
Briefly pressing the snap button once again
stops the video recording.
Fig. 18 Inserting SD / SDHC card
Videos are recorded in MPEG-4 advanced video coding format (H.264) and saved as a .h264
file. The videos can be played back using standard software, e.g. with the VLC player.
30 415510-7244-001 12/2014
3.3.3.2 Data Transfer to the PC via USB 2.0 Port
The USB port is used for data transfer to the PC. A mini USB 2.0 cable is required for transferring data to
the PC.
The built-in camera is compatible with ZEN or ZEN lite.
• Insert the mini USB plug (Fig. 19/1) of the USB cable into the USB 2.0 port (Fig. 19/2) on the reverse
side of microscope.
• Insert the USB 2.0 plug (Fig. 19/3) into the PC (Fig. 19/4).
Fig. 19 Data transfer to the PC via USB port
12/2014 415510-7244-001 31
3.3.3.3 Visualization of Data via HDMI Port
The integrated camera can be connected to a monitor, TV or projector for visualization of the live image
data.
The data at the HDMI output is in DVI format. Further HDMI functions (e.g. audio,
communication between monitor and camera) are not supported.
To enable the display the high-resolution images in HD format in this quality, it is
recommended that only HD-capable devices be connected.
• Connect the monitor / projector to the integrated camera using the HDMI cable.
To do this, insert the HDMI plug (Fig. 20/1) into the jack on the reverse side of the microscope
(Fig. 20/2) and the monitor / projector (Fig. 20/3).
Secure the HDMI plug on the jack on the monitor / projector.
Fig. 20 Connecting the integrated camera to a monitor / projector
If a monitor / projector is used, an HDMI / DVI adapter cable (Fig. 20/4) or HDMI / DVI adapter
will be required for the reproduction of image data.
A default resolution of 720p60 has been set.
• For further camera settings using the on-screen display menu (OSD) see section 3.3.4.
Live image data can be documented by photo or video recording, see section 3.3.3.1.
• Connect the integrated camera to the mains supply, see section 3.1.10 on page 27.
Direct generation of diagnostic results from these image is not proposed.
32 415510-7244-001 12/2014
3.3.3.4 Integrate the Camera into the Network via an Ethernet Port
The integrated camera has a fast Ethernet port for communication and image data transfer.
• Integrate the camera into the network via the fast Ethernet port.
To do this, insert the Ethernet cable (Fig. 21/1) into the jack on the reverse side of the microscope
(Fig. 21/2).
A WLAN router will be required to use the built-in camera together with the ZEISS Labscope
1 2
app. Existing WLAN infrastructure may be used or a separate microscope WLAN set up .
The integrated camera will identify itself automatically to the network (Fig. 21/3) on Dynamic
Host Configuration Protocol (DHCP) and will be automatically recognized by Labscope,
provided the iPad (Fig. 21/4) is in the same network.
Consult your network administrator for further guidance.
Fig. 21 Establishing a network connection
Further information on the Labscope app can be found at www.zeiss.com/labscope.

For an overview of all ZEISS Microscopy apps visit www.zeiss.com/micro-apps.
1
Prerequisite: High-performance 802.11n WLAN with sufficient free bandwidth. In the event of an overloaded or slow
WLAN the live image of the built-in camera may be delayed or incorrectly displayed on the iPad.
2
For further information on this subject visit www.zeiss.com/micro-apps.
12/2014 415510-7244-001 33
3.3.4 Remote Control of Integrated Camera
Fig. 22 Remote control
No. Button Standard function Function in OSD menu

1 On Turn On / Off HDMI output Turn On / Off HDMI output
2 Enter Trigger automatic white balance OK (apply value)
3 Arrow Up Increase contrast Up / increase value
Arrow Down Reduce contrast Down / reduce value
Arrow Left Reduce brightness Left
Arrow Right Increase brightness Right
4 Index None Go to top tier of menu
5 Live Clear screen of windows Exit menu
6 Input Toggle between 720p60 and 1080p30 outputs Toggle between 720p60 and 1080p30
outputs
7 Play Clear screen of windows Exit menu

8 Record Start / Stop recording video Start / Stop recording video
9 Capture Capture an image to SD card Capture an image to SD card
10 Menu Activate menu Exit menu
Table 4 Key assignment standard function / function in OSD menu of remote control
34 415510-7244-001 12/2014
3.3.5 Configuring the Integrated HD IP Camera
• Press the Menu button on the control panel.

The OSD menu for adjusting the settings will appear.
The following menus are available for configuring the camera:
Fig. 23 Color menu
AWB The purpose of white balance is to calibrate the camera to the color temperature of the light
source used. The AWB function of the integrated camera supports the Continuous / Push
button modes.
In Continuous mode the white balance is carried out continuously. In certain specimens this may
result in incorrect colors.
To ensure that the white balance applies to the light source and not the main color of the
specimen, the Push button mode can be set. Here the white balance is carried out only once if
the AWB / OK button is pressed outside the OSD menu.
Color temp. This creates a white balance bias towards yellow (left) or blue (right). If AWB (see above) is set to
push button, the effect of this regulator is only visible after a white balance has been carried out
with the AWB button (outside the OSD menu).
Saturation The camera's images are in sRGB color space with an 8 bit color depth per channel. The color
saturation per RGB value can be adjusted.
Extreme left: Gray scales
Extreme right: Maximum color saturation
Gamma The gamma correction of the camera can be adjusted. A choice of Gamma 1 and 0.45 is available.
12/2014 415510-7244-001 35
Fig. 24 Exposure menu
AET / AGC AET (automatic exposure time) and AGC (auto gain control) automatically ensure the correct
brightness of the image when the setting is at Continuous.
The settings are Once / Continuous / Off.
In some situations (e.g. strong contrasts on the specimen) the automatic setting may cause the
image to be under- or overexposed. In this case the automatic setting can be deactivated by
switching to Off.
A shorter exposure time with higher amplification (= more noise, but smoother live image) or a
longer exposure with lower amplification (= less noise, less smooth live image) can be selected in
Off mode.
In Once mode a one-time automatic adjustment will be carried out when confirmed with OK,
but then deactivated again.
Auto level Influences the AET / AGC control when Continuous mode is set.
Extreme left: very dark image
Extreme right: very light image
Exposure The exposure time can be set within the range 20 µs to ~2s. The exposure time may be set
automatically or manually. Manual setting is possible by selecting the AET / AGC control in Off
mode.
Extreme left: 20 µs
Extreme right: 2000 ms (2 s)
Gain The sensor amplification function can be set within the range 1x to 8x. Sensor gain may be set
automatically or manually. Manual setting is possible by selecting the AET / AGC control in Off
mode.
Extreme left: no increased amplification
(less image noise, less light sensitivity of sensor)
Extreme right: maximum amplification
(strong image noise, high light sensitivity of sensor)
36 415510-7244-001 12/2014
Fig. 25 Resolution menu
Preset The following options are available:

720p60: HD resolution, 1280x720, 60 Hz
1080p30: Full HD resolution, 1920x1080, 30 Hz
A default resolution of 720p60 has been set.
Please observe the following: Prior to conversion to the full HD resolution (1080p30), check that
the monitor supports this option. If 1080p30 has already been activated and the monitor displays
no image, please follow the instructions as described in section 3.13 on page 47.
Video Encoder The live image and video recordings are influenced by this setting. The following settings are
possible:
FPS:HALF BR:HIGH: Half frame rate, high data rate (8 Mbit / s)
FPS:HALF BR:MID: Half frame rate, medium data rate (4 Mbit / s)
FPS:HALF BR: LOW: Half frame rate, low data rate (2 Mbit / s)
FPS:FULL BR:HIGH: Full frame rate, high data rate (8 Mbit / s)
FPS:FULL BR:MID: Full frame rate, high data rate (4 Mbit / s)
FPS:FULL BR:LOW: Full frame rate, low data rate (2 Mbit / s)
Half frame rate results in improved image quality with the same data rate.
Sharpening Value range: 0 to 3.
Edge flickering may occur in some HDMI monitors with a default value of 3, but this will
disappear with lower values.
Snapshot Res The snapshot resolution for exposures to the SD card can be defined here.
(Res = resolution) The following modes can be set:
 as monitor on the HDMI port:
- 1920x1080 (16:9 side ratio), when the camera is in 1080p mode
(see default settings)
- 1280x720 (16:9 side ratio), when the camera is in 720p mode
(see default settings)
Advantages: Snap lasts < 1 second, small file sizes due to comparatively low
resolution
 5 MP:
- 2560x1920 (4:3 side ratio) – full sensor resolution, 5 megapixels
Advantages: full resolution, wider vertical image field
 2560x1440
- 2560x1440 (16:9 side ratio)
Advantages: high resolution (3.7 megapixels), same image field as in live image
Snapshot Delay This setting is useful when images are to be captured using the snap button on the tube. Because
pressing the button causes the microscope to shake, motion blur would be visible in the image
with immediate exposure. This effect is eliminated by snapshot delay.
The snapshot delay can be set between 0 and 10 seconds.
12/2014 415510-7244-001 37
Fig. 26 Setup User menu
Date / Time Date and time in year-month-day / hour-minute-second format.

Each element can be adjusted using the up / down buttons. The new time can be set by pressing
the OK button. Date and time are integrated into the folder and file names on the SD card.
Flip image The following modes are available for image mirroring:
FLIP H+V: horizontal and vertical mirroring
FLIP V: only vertical mirroring
FLIP H: only horizontal mirroring
OFF: no mirroring
Default setting: the image position on the monitor or iPad corresponds to that in the eyepiece.
Load Settings The previously saved user settings (USER 1-4) can be loaded in this menu.
Selecting FACTORY DEFAULT reinstates the default camera settings. After confirmation with OK
the camera will be restarted. This may take several seconds.
If the OSD menu is not available, the factory settings can be reinstated using the short-cut Snap
+ AWB / OK (keep depressed for two seconds).
Save Settings The current settings can be saved in a user storage location (USER 1-4).
They can be automatically loaded in the Load Settings (see above) or Startup Set menus (see
below) at a later point in time or each time the camera is switched on
Please observe the following: If the settings are saved, Startup Set is automatically set to this
storage location.
Startup Set This setting determines which user storage location (USER 1-4) is to be loaded each time the
camera is switched on.
Fig. 27 Technical Info menu
Version Current firmware version

MAC MAC address of the built-in camera
IP Currently used IP address
Destination IP Currently used multicast target address
Netmask Currently used netmask
38 415510-7244-001 12/2014
3.4 Switching the Microscope On / Off
• Switch on the microscope using the rotary knob (Fig. 28/2) (ON or AUTO position).
− ON position:
The two ON/OFF switches (Fig. 28/1) on the specimen stage for quickly switching the transmitted-
light illuminator on and off are inactive. The illuminator remains continuously switched on.
− AUTO position:
The ON/OFF switches (Fig. 28/1) for quickly switching the transmitted-light illuminator on and off
are active. So, the illuminator can be switched off and on again quickly. After an operation time of
15 minutes, the illuminator switches off automatically and goes to standby mode. Upon actuating
one of the ON/OFF switches, the illuminator switches on again. When actuating the switch once
more during operation, the 15-minute period will start again from the beginning.
Recommendation:
If you want to use the microscope only for a short time, you should use the AUTO function.
This saves energy and extends the lifetime of the transmitted-light illuminator.
Fig. 28 Switching the microscope on or off
12/2014 415510-7244-001 39
• Adjust the desired illumination intensity using the rotary knob (Fig. 11/11).
The selected intensity is indicated in five steps by the blue light-emitting diodes (Fig. 11/24) arranged on
both sides of the stand.
• The torque of the coarse focusing drive is factory-adjusted and can be readjusted if required. For this
purpose, insert a screwdriver into one of the four radially arranged holes of the ring (Fig. 11/20), hold
the coarse focusing knob (Fig. 11/21) in position and turn the ring with the screwdriver to the right or
left in order to adjust the torque of the coarse drive as desired.
• After finishing work, switch off the microscope with the rotary knob (Fig. 28/2) (OFF position).
• Cover the microscope with the dust cover.
3.5 Adjusting Interpupillary Distance

and Viewing Height
• Rotate the eyepiece tubes symmetrically toward

or away from one another to adjust the
distance between the tubes to your individual
interpupillary distance (Fig. 29).
With the correct interpupillary distance you see
only one round image while looking through both
eyepieces!
Fig. 29 Adjusting the interpupillary

• Swivel the eyepiece tubes upward (Fig. 30/A) or
distance downward (Fig. 30/B) to adjust the viewing
height to your individual requirements.
Fig. 30 Adjusting the viewing height
40 415510-7244-001 12/2014
• With the stand model Primovert with ergotube

the tube angle can be adjusted in the range
from 30° to 60° according to individual
requirements.
3.6 Compensating Defective Vision at

the Eyepieces and Inserting the
Eyepiece Pointer or Eyepiece
Micrometer
The eyepieces (Fig. 32/3) can be equipped with

fold-over rubber eyecups (Fig. 32/1: pulled out;
Fig. 32/2: folded over). Die eyecups are supplied Fig. 31 Tilting range of ergotube
together with the eyepieces, but are not yet
mounted.
Both eyepieces are suitable for spectacle wearers.
They contain additionally a focusing ring for the
compensation of defective vision. The provided
diopter scale serves to facilitate finding the correct
setting.
If required, an eyepiece pointer or an eyepiece
micrometer with a diameter of 23 mm can be
inserted in one eyepiece.
To this end, follow this procedure:
• Unscrew the stop (Fig. 32/5) by hand from the
eyepiece.
• Insert the eyepiece pointer (Fig. 32/4a) or the
eyepiece micrometer (Fig. 32/4b) into the
eyepiece (with the coated side facing your eyes).
Fig. 32 Inserting the eyepiece pointer or
• Screw in the stop again. eyepiece micrometer
• Insert the eyepiece into the tube.

• Attach the eyecups.
• Turn the focusing ring of the eyepiece (Fig. 32/3) to focus on the wedge-shaped figure of the eyepiece
pointer.
• Put the specimen onto the specimen stage. Look at the specimen through the eyepiece with the
eyepiece pointer and bring the microscopic image into focus using the focusing drive.
• When in the above-mentioned eyepiece both the microscopic image and the eyepiece pointer appear
sharply defined, focus the image for the second eye by turning the focusing ring of the second eyepiece.
Having done so, both microscopic images inclusive of the eyepiece pointer are focused.
From now on, you should focus onto the specimen using the focusing drive only.
12/2014 415510-7244-001 41
3.7 Adjusting Transmitted-Light Phase

Contrast
• First, adjust the microscope as you do for

brightfield.
• Turn the nosepiece to position the phase-
contrast objective (for Ph 0, Ph 1 or Ph 2) into
the light path.
• Open the aperture diaphragm completely by
means of the lever (Fig. 33/3) on the condenser.
• Introduce the slider (Fig. 33/1) into the
condenser so that the phase stop position
suitable for the objective used (Ph 0, Ph 1 or
Ph 2) is in the light path. Pay attention to the
correct detent position.
• Adjust the illumination intensity as needed.
Fig. 33 Inserting the slider Stand with Binocular Tube or Phototube

• Check the centering of the phase stop
according to the drawing in Fig. 34. To this end,
remove one eyepiece and replace it by the
diopter.
• Center the phase stop, if necessary (Fig. 34/A),
by turning the two adjusting screws (Fig. 33/2)
of the corresponding slider position by means
of the two Allen keys SW 1.5 (Fig. 33/4) until
the image corresponds to that shown in
Fig. 34/B.
• Afterwards, replace the diopter by the eyepiece
Fig. 34 Centering the phase stop
again.
Stand with Monitor

• Put on the standard phase contrast specimen.
• Use the two adjusting screws to center the
annular diaphragms on the monitor display,
while taking the following into account:
− Ensure even monitor display illumination for
the purpose of coarse adjustment.
− For fine adjustment, set the contrast to
maximum, so that the texture is as bright as
possible, while the background is as dark as
possible.
42 415510-7244-001 12/2014
3.8 Reflected-Light Fluorescence
• Turn the transmitted light / reflected light

changeover switch (Fig. 35/1) upward to the
reflected-light position (Fluorescence).
• Switch on the reflected-light illuminator using
the rotary knob (Fig. 35/2) and adjust the
desired illumination intensity.
Fig. 35 Adjusting reflected-light

fluorescence
3.9 Retrofitting the Microscope
Unplug the plug-in power unit from

line power before retrofitting the
microscope.
3.9.1 Replacing the Halogen Bulb

6 V / 30 W of the Transmitted-Light
Illuminator or the Transmitted-
Light Illuminator with LED
Switch off the microscope before

replacing the transmitted-light
illuminator with LED or the halogen
bulb 6 V / 30 W, allow for a sufficient
cool-down time and remove the plug-
in power unit from the socket.
• Press down the clamping lug of the cover
Fig. 36 Replacing the halogen bulb
(Fig. 36/1) and remove the cover from the 6 V / 30 W or the transmitted-light
stand. illuminator with LED
• Loosen both fastening screws (Fig. 36/2) of the
transmitted-light illuminator (e.g. transmitted-
light illuminator with halogen bulb, Fig. 36/3).
In doing so, slightly press the screws against the
spring and turn them by 90°: Turn left and right
screw clockwise.
• Pull the transmitted-light illuminator (Fig. 36/3) out of the stand.
• Pull the halogen bulb (Fig. 36/4) out of the lamp carrier and insert the new halogen bulb. Do not
touch the new lamp with bare fingers as this will decrease the life of the bulb.
12/2014 415510-7244-001 43
A spare bulb (Fig. 36/5) can be placed in the transmitted-light illuminator with halogen bulb
and remain there during the operation.
The transmitted-light illuminator with LED has to be replaced completely. The LED alone
cannot be replaced.
• Push the transmitted-light illuminator (e.g. transmitted-light illuminator with LED, Fig. 36/6) into the
stand and fasten it with the two screws (Fig. 36/7). In doing so, slightly press the screws against the
spring and turn them by 90°: Turn right and left screw counterclockwise.
• Attach the cover (Fig. 36/1) again to the stand and let it snap into place.
3.9.2 Attaching a Camera to the Stand

with Binocular Phototube
You can attach a digital camera, a video camera or

a compact digital camera of your choice to the
microscope with binocular phototube using the
four available camera adapters (see Section 2.1).
• Loosen the clamping screw (Fig. 37/7) and
remove the dust cap from the camera port
(Fig. 37/8) of the binocular phototube.
Attaching a Compact Digital Camera

• Sliding mount (Fig. 37/4), thread adapter ring
M37/52 (Fig. 37/3) and lens mount (Fig. 37/6)
are supplied pre-assembled as Digital Camera
Adapter P95 M37/52x0.75. The opposite
drawing shows this unit dismantled.
Furthermore, you may unscrew the thread
adapter ring M37/52 (Fig. 37/3) from the sliding
mount (Fig. 37/4) (not shown) so that you can
also attach cameras with M37 thread.
• Mount the adapter ring (Fig. 37/2) (optional
accessory for the corresponding camera) to the
Fig. 37 Attaching the camera camera (Fig. 37/1) (see the operating
instructions of the camera).
• Screw the unit consisting of sliding mount (Fig. 37/4), thread adapter ring M37/52 (Fig. 37/3) and lens
mount (Fig. 37/6) into the adapter ring (Fig. 37/2).
• Insert the camera with adapter into the phototube as far as it will go. Align it and fasten it with the
clamping screw (Fig. 37/7).
44 415510-7244-001 12/2014
• Depending on the microscope equipment or the camera used, it may be necessary to optimize the
distance between camera lens and lens mount (Fig. 37/6) (see double-headed arrow). This will be
necessary in particular if it is impossible to obtain an unvignetted image in any of the zoom positions
of the camera lens. To this end, adjust the camera as follows:
− Switch off autofocus.
− Set the object distance to ∞.
− Set aperture-priority auto exposure mode.
− Choose an aperture as large as possible (i.e. small aperture number!).
Not all cameras provide these options. Please consult the operating manual of the camera used.
• Loosen the set screw (Fig. 37/5).
• Vary the camera lens/lens mount distance gradually, i.e. displace the sliding mount with camera on the
lens mount in defined steps.
• Zoom the camera lens through from wide angle (W) to tele position (T).
• Carry out this test until the image is format-filling without masking or vignetting.
• Retighten the set screw (Fig. 37/5).
With camera/adapter combinations that have not been recommended expressly by Zeiss, it
may be quite impossible to obtain an unvignetted image.
Attaching a Digital Camera, Single-Lens Reflex Camera or Video Camera with C-Mount Thread
Cameras with C-mount thread are to be connected to the phototube of the microscope by means of the
camera adapter P95-C 2/3" 0.65x or P95-C 1/2" 0.5x (Fig. 37/9).
• Put the video camera (Fig. 37/10) or single-lens reflex camera (Fig. 37/13) (using the T2 adapter for
this camera (Fig. 37/12) together with the corresponding camera adapter (Fig. 37/9 or 11) into the
phototube as far as it will go, align it and fasten it with the clamping screw (Fig. 37/7).
12/2014 415510-7244-001 45
3.10 Care, Maintenance and Troubleshooting
3.11 Instrument Care
Care of the microscope is restricted to the following operations:

• Cover the instrument with the dust cover after every use and do not expose the instrument to
ultraviolet radiation without any protection.
• Do not install the microscope in a humid room; maximum humidity < 75%.
• Cover open tubes with the dust caps.
• Remove dust and loose dirt from visible optical surfaces with a brush, blower brush, cotton swab,
optics cleaning tissue, or a cotton cloth.
• Remove water-soluble dirt (coffee, cola, etc.) by blowing on it and then wiping it off with a dust-free
cotton cloth or a cloth moistened with water to which you may also add a mild detergent.
• Wipe off stubborn oily or fatty dirt (immersion oil, fingerprints) with cotton cloths or a dust-free cotton
cloth moistened with the optics cleaning solution L.
The cleaning solution consists of 90 vol% gasoline and 10 vol% isopropanol (IPA). Its individual
constituents are also known as:
Gasoline: Surgical spirit, petroleum ether
Isopropanol: 2-propanol,
dimethyl carbinol,
2-hydroxypropane
Clean optical surfaces by polishing them in circles from the middle and to the edges using slight pressure
only.
Before cleaning the plug-in power unit, disconnect it from line power. Avoid in any case the penetration
of moisture into the plug-in power unit.
For using the microscope in warm and humid climatic zones, it comes with all optical components already
protected against fungus attack.
46 415510-7244-001 12/2014
3.12 Troubleshooting on the Microcope
Problem Cause Remedy

Field of view is not completely Nosepiece with objective has not been Switch nosepiece with objective into
visible. switched into click-stop position. click-stop position.
Condenser has not been set correctly. Set condenser correctly.
Filter slider has not been inserted Push filter slider to click-stop position.
correctly into the filter mount.
Slider for phase contrast has not been Centering the phase stop for phase
adjusted correctly. contrast, push the slider to click-stop
position.
Low resolving power, poor Aperture diaphragm has not been Open the aperture diaphragm to 2/3 for
image contrast. opened to correct size. brightfield, or completely for phase
contrast.
Use of wrong cover glass thickness for Use standard 1 mm or 0.17 mm cover
transmitted light objectives corrected glass.
for 1 mm or 0.17 mm cover glass.
Dirt or dust on the optical surfaces of Clean the respective optical
objectives, eyepieces, condensers, or components.
filters.
Major focus differences after Diopter setting of the eyepieces has Adjust the diopter setting of the
changing the objective. not been correctly adjusted. eyepieces, so it matches your visual
acuity, or set it to zero when using
glasses or contact lenses.
Objectives has not been correctly Screw in objectives correctly.
screwed in.
The 6 V 30 W halogen bulb or Power plug has not been inserted into Insert power plug into power outlet,
the LED source does not light power outlet, plug-in power unit has connect plug-in power unit with stand.
up although the microscope not been connected with stand.
has been switched on.
6 V 30 W halogen bulb or LED source Replace the defective 6 V 30 W halogen
is defective. bulb or the LED module.
Pins of the 6 V 30 W halogen bulb Insert the pins of the 6 V 30 W halogen
have not been inserted properly into bulb properly into the lamp holder.
lamp holder.
Transmitted-light illuminator has not Tighten fastening screws of the
been properly screwed in. transmitted-light illuminator.
The 6 V 30 W halogen bulb is End of average service life of 6 V / Replace the 6 V 30 W halogen bulb.
flickering, its light intensity is 30 W halogen lamp has been
unstable, the illumination is reached.
inhomogeneous.
Power cable has not been installed Connect the power cable correctly or
correctly or broken. replace it.
Pins of 6 V 30 W halogen bulb has Insert the pins of the 6 V 30 W halogen
not been inserted correctly into lamp bulb correctly into the lamp holder.
holder.
Pins of 6 V 30 W halogen bulb not Insert the pins of the 6 V 30 W halogen
inserted symmetrically into lamp bulb symmetrically into the lamp holder.
holder.
12/2014 415510-7244-001 47
Problem Cause Remedy

Image focus is unstable. Adjusted torque of coarse focusing Increase the torque of the coarse
drive is too low. focusing drive to make motion stiffer.
The fluorescence light source Fluorescence light source or circuit Contact service person for further
doesn't lighten on. board is defective. checking. If necessary, change LED
source or PCB.
Replacement requires special tools and
can thus not be carried out by the
customer.
Greenish background when The yellow filter is not inserted. Insert filter slider with yellow filter
working in fluorescent The transmitted LED phosphor (which is a part of the standard delivery)
illumination mode. generates fluorescent background. whenever changing to fluorescence
mode.
48 415510-7244-001 12/2014
3.13 Troubleshooting on the Integrated HD IP Camera
Problem-solving notes for the use of the built-in camera in conjunction with the network / WLAN and Labscope app
are to be found separately at the Zeiss website www.zeiss.com/labscope.
Problem Cause Troubleshooting

LED does not light The camera does not draw power via Check the power supply.
the stand power cable. Check if stand power is on.
If it is in auto mode, try to press auto
on/off button on stage.
Cable is not connected to a certified Requirements: 5 V DC with at least
PSU. 950 mA at output.
Cable is not suitable. Be sure that Use the supplied original cable.
power is on. Or either LED or camera Contact service to further check.
is broken.
If LED is broken, change button control
PCB.
If camera is broken, replace the camera.
Replacement requires special tools and
can thus not be carried out by the
customer.
LED flashes red The camera indicates an error. SD card is full:
Insert a new card or delete images from
the current card,
SD card is write-protected:
Push the locking slide on the card to
"unlocked".
SD card is defective or not formatted:
Format SD card; replace SD card if error
message does not disappear
Buttons do not react, LED The buttons are temporarily locked, as Control the camera via PC software or
flashes red when a button is the camera is being accessed Zeiss app, or close the PC software or
pressed. exclusively from the PC or network. Zeiss app.
Firmware update not For an update an SD card must be Insert a formatted, unsecured SD card
functioning. inserted and the firmware update with at least 10 MB free memory space.
must be saved to a specified subfolder Be sure to carefully follow the
on the SD card. instructions enclosed with the firmware
update.
The live image of the camera is The camera is not recognized by the Several cameras have been connected to
not displayed in ZEN. Zeiss imaging software. the PC: select the correct camera
"AxioCamER" from the menu list.
Newly captured images are not Microsoft Windows Explorer has not Remove the memory card from the
visible in Microsoft Windows updated the contents of the card. camera, wait for three seconds and
Explorer on the memory card. re-insert it.
Microsoft Windows Explorer will read in
the contents of the card again.
The camera "forgets" the The buffer battery is empty. Contact service to replace the buffer
date / time. battery. Replacement requires special
tools and can thus not be carried out by
the customer.
12/2014 415510-7244-001 49
Problem Cause Troubleshooting

The image has severe noise. The amplification (gain) is set too Reduce the gain and increase the
high. exposure time instead.
The image is too dark or too AET / AGC (automatic exposure time / Activate AET / AGC and select
light. automatic gain control) has not been Continuous mode or manually adjust
activated. the exposure time so that the setting is
correct for the current light situation.
Monitor connected via The camera is not delivering a signal, Monitor switches to stand-by mode:
HDMI / DVI does not display an or signal is not compatible with the Ensure that the camera has been
image. monitor. switched on for at least 30 seconds and
the LED lights up green. Check the plug
connections on the tube and monitor.
Monitor displays an error message that
after switch-over to 1080p resolution
the signal is outside the monitor
specifications.
The monitor may not support a 1080p30
signal. Hold down the Menu and OK
buttons for two seconds to return the
camera to 720p60 resolution.
The camera forgets the settings Settings were not stored. If settings have been made for regular
(e.g. manual white balance, use, they must be saved before
resolution) if the power supply disconnecting the power.
is disrupted.
Camera otherwise behaves The camera may have been brought Restore all default camera settings via
abnormally. into a non-intended state. the OSD menu or by pressing the Snap
and AWB / OK buttons for two
seconds. The camera will then restart
and should be in a normal state.
When recording a video to the The "Snap" button was pressed too To record a single video, press and hold
SD card, the camera generates long. the "Snap" button and release it as soon
several short video files. as the LED starts blinking.
50 415510-7244-001 12/2014
Primovert Technical Data ZEISS
4 TECHNICAL DATA
Dimensions (width x depth x height)
Primovert approx. 261 mm x 550 mm x 494 mm
Primovert HDcam approx. 215.5 mm x 473 mm x 494 mm
Primovert iLED approx. 215.5 mm x 552 mm x 494 mm
Weight (without accessories and packaging)
Primovert approx. 11 kg
Primovert HDcam approx. 11 kg
Primovert iLED approx. 11.5 kg
Ambient Conditions
Transportation (in packaging):
Admissible ambient temperature -40 to +70 °C
Storage:
Admissible ambient temperature +10 to +40 °C
Admissible air humidity max. 75 % at 35 °C (no condensation)
Operation:
Operating environment closed rooms
Operating altitude max. 2000 m
Admissible ambient temperature +10 to +40 °C
Admissible air humidity max. 75 % at 35 °C (no condensation)
Atmospheric pressure 800 hPa to 1060 hPa
Operating Data
Protection class II
Protection type IP20
Electrical safety in compliance with DIN EN 61010-1 (IEC 61010-1)
including CSA and UL directives
Pollution degree 2
Overvoltage category II
Radio interference suppression in compliance with EN 61326-1
Line voltage 100 to 240 V (±10 %) wide-range input power supply, i.e.
voltage setting of the instrument need not be changed!
Line frequency 50 / 60 Hz
Power consumption Primovert HDcam max. 45 W

Power supply Primovert HDcam Input: 100-240 V DC; 50-60 Hz, max. 1.6 A
Power supply Primovert HDcam Output: 12 V DC, max. 5 A
Power consumption Primovert iLED max. 30 W

Power supply Primovert iLED Input: 100-240 V DC; 50-60 Hz, max. 0.7 A
Power supply Primovert iLED Output: 12 V DC, max. 2.5 A
Microscope 12 V / 6 V DC adjustable from 1.5 V to 6 V

LED class of complete device Risk group 2 in compliance with IEC 62471
12/2014 415510-7244-001 51
ZEISS Technical Data Primovert
Light Sources
Halogen lamp HAL 6 V, 30 W
Adjustability of light source continuous, from 1.5 to 6 V DC
Color temperature at 6 V 2800 K
Luminous flux 765 lm
Average life 100 h
Luminous area 1.5 x 1.5 mm
iLED illumination
Fluorescent illumination blue LED, peak wavelength 470 nm, LED risk group 2 according
to IEC 62471
Homogeneous filed illumination 20 mm
Analogous brightness adjustment from approx. 15 to 100 %
LED illumination White light LED, LED risk group 2 according to IEC 62471
Constant, brightness-independent
color temperature 7000 K
Homogeneous field illumination 20 mm diameter
Suitable for objectives with
magnifications of 4x to 40x
Analogous brightness adjustment from approx. 15 to 100 %
Optical/Mechanical Data
Stand with stage focusing drives

With coarse focusing drive 45 mm/rev.
With fine focusing drive 0.5 mm/rev.
Total stage lift 15 mm
Objective change manual via quadruple nosepiece
Objectives infinity-corrected objective range of Primo class with W 0.8
mounting thread
Eyepieces 30 mm tube diameter
with field-of-view number 20 WF 10x/20 Br. foc.
Specimen stage fixed
Dimensions (width x depth) 200 x 239 mm
Specimen guide right side
Verniers with numerical and alphabetic X direction: numerical scale, readable from right to left
scale Y direction: alphabetic scale, readable in the mirror
Coaxial drive right side
LD condenser 0.3 for Vobj 4x to 40x, a = 72 mm
LD condenser 0.4 for Vobj 4x to 40x, a = 55 mm
Primovert with binocular tube
Maximum field-of-view number 20
Interpupillary distance adjustable from 48 to 75 mm
Tube angle 45°
Viewing height 350 to 390 mm
Viewing port tube factor 1x
52 415510-7244-001 12/2014
Primovert Technical Data ZEISS
Primovert with binocular phototube

Tube angle 45°
Photo/video port tube factor 1x, 60 mm mount
Fixed beam splitting 50 % vis / 50 % doc
Primovert with binocular ergotube
Tube angle 30° to 60°, continuously adjustable
Primovert with integrated 5 MP HD IP camera
Acquired visual field of the camera 11.4 mm x 8.56 mm (14.2 mm diagonal)
Fixed built-in camera adapter 0.63x
Output HDMI / USB2.0 / Ethernet port/ SD card
iPad holder tiltable 40-80 degree
Primovert with integrated fluorescence unit
Illumination Reflected illumination (Fluorescent) / Transmitted illumination
Fluorescent source LED central wavelength 470 nm
Transmitted source LED 7000 K
Interpupillary distance adjustable from 48 mm to 75 mm
Tube angle 45°
Viewing height 350 mm to 390 mm
Photo / video port tube factor 1x, 60 mm
Fixed beam splitting mount 50 % vis / 50 % doc
HD-CMOS-camera
Sensor-specific data
Sensor Micron MT9P031
Sensor size 1/2.5", 5.7 mm x 4.28 mm (7.1 mm diagonal)
Pixel size 2.2 µm x 2.2 µm
Sensor type 1/2.5" CMOS, color
Readout mode Progressive scan
No. of pixels (H x V), full field 2560 x 1920 pixels active, 5 megapixels
Live image, movie 1920 x 1080 pixels, 30 Fps (H264 max. 16 Mbits / s)
Spectral sensivity (without IR filter) 400 nm to 700 nm
12/2014 415510-7244-001 53
ZEISS Technical Data Primovert
Signal processing / interface-specific data
Digitization / color depth 24 bit, 3 x 8 bit / pixel

Amplification 0-18 dB
Interfaces USB 2.0 mini USB plug
LAN via RJ 45 jack, 100 Mbit
SD card (secure digital) 1-32 GB, slot for SD and SDHC
HDMI (1080p/30 or 720p/60)
Remote release IR sensor
Duo LED Ready (green), exposure (flashing green), not ready (red), error
(flashing red)
Key matrix White balance, snap, contrast, brightness, menu
Exposure time 10 µs to 2 s
54 415510-7244-001 12/2014

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Primovert

Issued by: Carl Zeiss Microscopy GmbH

Carl Zeiss Microscopy GmbH

Manual number: 415510-7244-001

3.9.2 Attaching a Camera to the Stand with Binocular Phototube ............................................... 44

1 NOTES ON INSTRUMENT SAFETY

1.1 General Safety Notes

1.2 Instrument Safety and EMC

1.3 Unpacking, Setup, Transportation, Storage

− Keep transport and storage temperatures as specified in the Technical Data.

1.6 Warranty Notes

Please consider the following warranty notes for the microscopes:

1.7 Warning and Information Labels

Fig. 1 Warning and information labels on the Primovert

2.1 System Overview

2.2 Intended Use

2.3 Instrument Description and Main Features

− Quadruple nosepiece with W 0.8" lens thread running on ball bearing.

LD Plan-ACHROMAT 40x/0.5 Ph 1 ∞/1.0

3 START-UP AND OPERATION

3.1 Starting up the Microscope

3.1.1 Setting up the Microscope

3.1.2 Screwing in the Objectives

• Remove the dust caps from the location holes

Fig. 3 Screwing in the objectives

3.1.3 Inserting the Condenser

• Remove the condenser cap.

3.1.4 Inserting the Filter Slider

• Put the color filter (Fig. 5/1) or the green

When using the Primovert iLED in

3.1.5 Inserting the Phase Contrast Slider

The 2-position Ph slider (Fig. 6/1) and the

If necessary, the neutral filter can be

Fig. 6 Inserting the phase contrast slider

3.1.6 Inserting the Stage Inserts and

• Insert either the metal insert (Fig. 7/5) or the

3.1.7 Mounting the Specimen Guide and

• If attached, remove the stage enlargement plate

3.1.8 Mounting UV Protection Plate onto

• Put the UV protection plate (Fig. 9/1) on the

3.1.9 Transmitted-Light Illuminator: Inserting the LED

3.1.10 Connecting the Stand with

• If necessary, replace the power supply adapter

3.2 Operating the Microscope

3.2.1 Operational and Functional Elements of the Primovert Microscope

Legend to Fig. 11:

Fig. 12 Operational elements of Primovert HDcam with integrated camera

Legend to Fig. 12:

Operating of Primovert HDcam with integrated camera with iPad

Fig. 14 Operational elements of Primovert iLED with integrated fluorescence unit

Legend to Fig. 14:

3.3 Primovert HDcam

3.3.1 Control and Functional Elements of the Integrated Camera

Do not operate the camera in a

• Briefly press the snap button (Fig. 15/8) to

Pos. No. Standard function Function in OSD menu

Table 1 Key assignment standard function / function in OSD menu

Direct generation of diagnostic results from these images is not proposed.

The camera has a number of ports: