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Lab Report 2 (Agr122)

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LAB REPORT 2

MORPHOLOGY AND ANATOMY OF DICOT AND MONOCOT PLANTS

NAME NURUL HANIM BINTI MUHAMT NOH


STUDENT ID 2021872162
CLASS M3AT1101H
LECTURER SHAMPAZURAINI BINTI SAMSURI
INTRODUCTION
Angiosperms is the type of plant kingdom which is a flowering plant. It is can be divided into
two main groups which is monocotyledonous (monocots) and dicotyledonous (dicots) plant.
Monocot plants is a plant that have only one cotyledon in the seed where dicot plants are a
plant that have two cotyledons in the seed. There are many differences between these two
types of cotyledons likes a pattern of their leaf, a pattern of their flower, a position root of
xylem and phloem and other than that. Moreover, there are some key features (morphological
and anatomical) that we can look to determine whether a plant is monocot or dicot.

EXPERIMENT 1: MONOCOT AND DICOT LEAF


Materials that we need to use:
 Monocot leaf
 Dicot leaf
 Microscope

The method is a very simple which is we just need to get a dicot and monocot leaf specimen
like a picture that provided above and see it under a microscope.
Results and Discussions:
The leaf of monocot and dicot that we see under microscope we will get like a picture
provided above. Monocot leaf epidermis was most thick than dicot epidermis leaf which is
cover with a waxy cuticle and lax chloroplast. The guard cell is also makes up in the majority
of all leaf tissues and it is including the photosynthetic cells and the veins which carry a both
xylem and phloem. In monocot, leaf the veins are typically travel in parallel and the
mesophyll more similar throughout. In contrast, the leaf of dicot tends to have a centriole
which carries the vascular tissue from the branches and travel throughout the leaf.

References :
 https://drive.google.com/file/d/1VsMwMDCFE6dNmNSU-rhWiL6ini1w2FnK/view?
usp=drivesdk
 https://drive.google.com/file/d/1u-cOsC1OiSoHbOqlVgbbdyenai8eGd2Y/view?
usp=drivesdk

EXPERIMENT 2: MONOCOT AND DICOT STEM


Materials that we need to use:
 Sample of monocot and dicot stem
 Sharp blade
 Watch glass
 Glass slaid
 Cover slip
 Brush
 Glycerine
 Safranine
 Microscope
Procedure or Method:
1) Take monocot and dicot stem, then cut the thin sections as much as possible with a
help of a sharp blade.
2) Cut at least 4-5 sections.
3) Keep the cut sections in watch glass containing water. (In this step, we must do it
carefully with the help of brush only).
4) Add some safranin to watch glass containing water to stain the selected sections.
5) Then, transfer the sections to fresh water to remove extra stain.
6) After that, transfer one very thin section on clean slide and remove extra stain from
section using a blotting paper.
7) Add a single drop of glycerine on the section and put the coverslip gently to avoid the
formation of bubbles.
8) Precautions:
 Do not overstain or understain sections
 Extra stain and glycerine should not come out of the slide, actually you can
use filter paper or tissue paper to clean it.

Results and Discussions :

There are have a similarities comparing at these stems of monocot and dicot , which include
the majority of flowering plants stem tissue composes the majority of the structure
surrounded by external empty terminals in both stem . The differences are in an monocot
these vascular bundles are smaller than vascular bundles in dicot .
References :
 https://drive.google.com/file/d/1UuitbA4nnT5E0MNYpdk0raIj2hY5XxMb/view?
usp=drivesdk
 https://drive.google.com/file/d/1f6mjTlYcLeeE736HHmoa9aKd6QDHI0wf/view?
usp=drivesdk
 https://drive.google.com/file/d/1jyxcnSAQqIlECKVnPFsy_ge-A8Lyf7OX/view?
usp=drivesdk

EXPERIMENT 3 : MONOCOT AND DICOT ROOT


Materials that we need to use :
 Monocot and dicot root
 Sharp blade
 Glass slide
 Cover slips
 Microscope
 Brush
 Watch glass
 Glycerine
 Safranine
Procedure or Methods :
1) Take monocot and dicot root , then cut the thin sections as much as possible using a
sharp blade .
2) Cut at least 4-5 sections .
3) Keep the cut sections in watch glass containing water. (In this step, we must do it
carefully with the help of brush only).
4) Add some safranin to watch glass containing water to stain the selected sections.
5) Then, transfer the sections to fresh water to remove extra stain.
6) After that, transfer one very thin section on clean slide and remove extra stain from
section using a blotting paper.
7) Add a single drop of glycerine on the section and put the coverslip gently to avoid the
formation of bubbles.
Results and discussions :

The roots of monocots and young dicots are similar in that is an outer epidermis , a cortex
and in endodermis known as the paricycle interior tooth . Bundles of xylem vessels and
bundles of phloem are set near the xylem.In monocots, vascular bundles are arranged in a
circular arrangement , while in dicot the xylem form the centriol x shaped formation . In dicot
, the pericycle and the parenchyma around the vascular fibers can form hair root vascular
cambium .
References :
 https://drive.google.com/file/d/1e0k9zaNSXiqiVetEA13mBMsBsAEN-Gtl/view?
usp=drivesdk
 https://drive.google.com/file/d/1bbr1tN0Lj_oxmou0iZpxRylQVvZwAehY/view?
usp=drivesdk

CONCLUSIONS

For conclusion for these three experiments , we can conclude that , for the first
experiment is about the leaf of monocot and dicot . From the experiment , we can see
that monocot leaf and dicot leaf have a differentiation in the size of epidermis , guard
cell , mesophyl palisade and others . In the experiment 2 which is we observe about a
monocot and dicot stem . In these experiment , we can see that a differentiation
between a monocot stem and dicot stem likes a cambium vascular , a position of
xylem and phloem and more . And the last one , we do an experiment about monocot
and dicot root . In monocot , it is arranged in circular where in dicot it is arranged
with an x shaped formation .

QUESTION

 List 6 types of monocot and dicot plants that available in your campus .
 Monocots : oil palm , olive , guava
 Dicots : durian , chili , coffee

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