Tem (Cse)
Tem (Cse)
Tem (Cse)
Transmission ELectron
Microscopy
GROUP 6
1 MANSI 00101012021
2 SAKSHI 01801012021
4 SIMMI 03101012021
https://youtu.be/a0G7iyz4McM
The given diagram
represents the working of
Transmission Electron
Microscope along with well
labelled diagram and the
arrows representing the
path of the electron beam.
● The electron gun consists of an electron source that produces electron beam travels via
anode in the column of the microscope.
● The Condensing lens are used to focus the electrons of the beam into a thin beam which is
allowed to fall on the specimen.
● On reaching the specimen, the specimen scatters the electrons. The beam of transmitted
electrons is directed to the magnetic objective lens and the aperture.
● The final image is produced due to transmitted beam when it is passed through the
Projective lens which helps in magnifying the image of the specimen.
Any high-resolution imaging technique has its own in-built limitation: at any given
time one can examine just a small part of a specimen; the higher the resolution,
the lower will be its sampling
2. Projection Limitation
4. Specimen Preparation
Specimens need to be thin - the materials have to be electron transparent.
This in turn means that electrons passing through the material and falling on
the screen or photographic plate must have sufficient intensity for generating
an image within a reasonable timeframe. This is often a function of the
electron energy and the average atomic number (Z) of the specimen under
observation.
Extending Capabilities of TEM to
overcome drawbacks
1. Cryogenic TEM
Cryogenic transmission electron microscopy (Cryo-TEM) is widely used in nanoparticle
research. The specimens are kept in vitreous ice so that they are close to their native
environment. Then they are imaged whilst maintained at the temperature of liquid helium or
liquid nitrogen. This process of specimen preparation decreases sample damage from
radiation by almost 6-fold. 3D images of large biological structures at nanometer resolutions
can also be obtained using this method of sample preparation. A great advantage of
Cryo-TEM is that the sample environment can be controlled and hence the image retains
the native structural features without any distortions.