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Agar from malaysian red seaweed as potential material for synthesis of

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Article in Journal of Engineering Science and Technology · July 2016

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Journal of Engineering Science and Technology
Special Issue on SOMCHE 2015, July (2016) 1 - 15
© School of Engineering, Taylor’s University

AGAR FROM MALAYSIAN RED SEAWEED AS

POTENTIAL MATERIAL FOR SYNTHESIS OF BIOPLASTIC FILM
1 2 3 2,
SIEW-LING HII , JIA-YEE LIM , WAN-TECK ONG , CHING-LEE WONG *
1
Department of Food Technology, School of Engineering and Technology, University
College of Technology Sarawak, 868 Persiaran Brooke, 96000 Sibu, Sarawak Malaysia
2
School of Biosciences, Taylor’s University, Taylor’s University Lakeside Campus, No. 1,
Jalan Taylor’s, 47500 Subang Jaya, Selangor DE, Malaysia
3
Department of Chemical Engineering, Faculty of Engineering and Science, Universiti
Tunku Abdul Rahman, Jalan Genting Kelang, 53300 Kuala Lumpur, Malaysia
*Corresponding Author: [email protected]

Abstract
The main aim of this study was to identify the potential use of agar extracted
from red seaweed, Gracilaria salicornia, collected from the coastal area of
Malaysia as the raw material for synthesis of bioplastic film. Agar was
extracted via two extraction methods: (1) alkali extraction method and (2) photo
bleaching extraction method. The yields of agar by both of the methods were 9
to 11 %. The alkali extracted agar (AEA) and photo bleached agar (PBA) were
incorporated as the raw materials for the formation of bioplastic films while
sago starch and glycerol were added to increase workability. Physicochemical
properties of the two bioplastic films were characterised. FTIR analysis
confirmed the presence of agar in both plastic films with the presence of 3,6-
anhydrogalactose residues and further indicated that the interactions of agar and
sago starch were strong in both PBA and AEA films. The results showed that
tensile strength and percent elongation of PBA film (3.067 MPa, 3.270 %) was
higher than AEA film (2.431 MPa, 2.476 %). Thermogravimetric analysis
(TGA; % residual weight) revealed that AEA film has higher thermal stability
(14.80 %) than PBA film (10.27 %) while rheological results proved that both
films exhibited non-Newtonian behaviors. The AEA film was completely
decomposed after 30 days in the soil burial test. Results of current study show a
wide range of future possibilities and commercial applications of AEA and
PBA bioplastic films.
Keywords: Agar, Gracilaria salicornia, Alkali extraction method, Photo bleaching
extraction method, Bioplastic film.

1
2 S. L. Hii et al.

Abbreviations
AEA Alkali Extracted Agar
Au/Pd Gold/Platinum
FTIR Fourier Transform Infrared
NaOH Sodium Hydroxide
PBA Photo Bleached Agar
SEM Scanning Electron Microscopy
TGA Thermogravimetric Analysis

1. Introduction
Nowadays, due to the depletion of petroleum sources and environmental
effects caused by the conventional plastics, bioplastics films are being studied
extensively as an alternative to conventional plastics because of their
excellent biodegradability, biocompatibility and edibility. There are a few
criteria for the formation of such films whereby at least one film forming
agent and plasticizer are to be added to a solvent to form a film forming
solution [1]. Some of the popular films forming agents are starch and
cellulose derivatives from plant sources, as well as alginate from seaweed
sources [2-5]. Other polysaccharides from seaweed (macroalgae) sources such
as agar and carrageenan are getting more attentions due to their ability to form
edible films as well as the ability of macroalgae to grow in harsh conditions
[6] and to be renewed annually.
Agar is a polysaccharide which can be easily found in some families of red
seaweed (Rhodophyceae), generally Gracilariaceae and Gelidiaceae. It consists of
two major components which are agarose and agaropectin [7]. It is also a
hydrophilic colloid which has the capability of forming reversible gels by cooling
it from a hot aqueous solution [8]. Agar is first used in the fields of food,
biotechnology and pharmaceutical applications before being introduced as a raw
material for production of bioplastic film formation. According to Phan et al. [9],
agar-based films possess transparent, strong and flexible characteristics at low
moisture content. In addition, it is heat-sealable which makes it a good application
for food packaging industry.
Conventionally, native agar was extracted by leaching the red seaweed in hot
water, filtering the extract, and followed by concentrating the extract using
freezing and thawing processes to eliminate water. This extraction method can be
easily used to extract agar from Gelidium species but not for agar with high gel
strength like Gracilaria species [7]. Pretreatment procedures are thus needed to
effectively extract the agar with higher gel strength with the formation of 3,6-
anhydrogalactose bridge [10]. Bleaching process is also incorporated during agar
extraction process in order to reduce the colour of agar. Current industrial practice
uses chemical (sodium hypochlorite) for bleaching process and it gives off
hazardous chlorine gas. Therefore, an eco-friendly technique that is known as
photo bleaching process was introduced in the study of Li et al. [11]. Through this
process, the coloured organic matters inside seaweed were subjected to
photochemical degradation [12].
Agar can be extracted mainly from red seaweed families Gracilariaceae and
Gelidiaceae. Gracilaria is the second largest genus of red seaweed and it is

Journal of Engineering Science and Technology Special Issue 7/2016

 Agar from Malaysian Red Seaweed as Potential Material for Synthesis of . . . . 3

comprised of more than 150 species distributed worldwide [13]. There are a
total of 20 Gracilaria species in Malaysia and one of the most common species
is Gracilaria changii, which grow abundantly in mangrove areas fringing the
west coast of Peninsular Malaysia [14,15]. The demand for Gracilaria changii
is high because of their high gel strength, proteins, fatty acids and bioactive
compounds which have wide applications for cosmetic, pharmaceutical and
food industries [16]. However, as a result of pollution, introduction of alien
species, climate change as well as over-harvesting, the supply of Gracilaria
changii become limited. Therefore, it is necessary to explore the potential
market of other Gracilaria species in Malaysia. In view of this, the objective of
this study is to investigate the agar quality from indigenous red seaweed,
Gracilaria salicornia, using both alkali extraction and photo bleaching
extraction methods. The characteristics of bioplastic film produced using the
extracted agar were determined.

2. Materials and Methods

2.1. Seaweed sampling and preliminary treatment
Specimens of red seaweed G. salicornia were collected at the beach of Port
Dickson, Malaysia. The collected seaweed was cleaned with tap water for several
times and dried at 60 ºC in an oven. The dried seaweed specimens were subjected
to size reduction before stored in air-tight bags with silica gels.

2.2. Seaweed extraction method

2.2.1. Alkali treatment extraction
Alkali treatment extraction method was carried out according to the method of
Chirapart et al. [17] with minor modifications. Dried seaweeds (10 g)
were treated with alkaline solution (500 mL of 5 %w/v NaOH) for 2 hours at
80 ºC. Following this, the alkali-treated sample was rinsed properly and placed
in deionized water (room temperature). The pH of sample was adjusted to a
range of 6.5 to 7.5. The sample was then heated at 120 ºC for 2 hours. The
filtrate of sample was left to cool to room temperature and frozen overnight to
concentrate the agar gel. The frozen solidified agar was thawed and dried at
50 ºC for 24 hours.

2.2.2. Photo bleaching extraction

Photo bleaching extraction method was carried out according to the method of Li
et al. [11] with minor modifications. The initial extraction method is similar with
alkali treatment extraction method with additional photo bleaching process. After
pH of the treated seaweed was adjusted to a range of 6.5 to 7.5, the seaweed
samples were soaked in distilled water and left overnight under fluorescent lamp
prior to photo bleaching process for 8 hours. Following this, the seaweed were
rinsed and concentrated according to the process as described in the alkali
treatment extraction method (Section 2.2.1).

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4 S. L. Hii et al.

2.2.3. Determination of agar yield

The yield of agar was determined based on the initial dry weight of the seaweed
and the final dry weight of the agar extracted, as shown in Equation 1.
dry weight of agar
Yield (% w/w) = × 100 % (1)
initial dry weight of seaweed

2.3. Preparation of bioplastic film

Bioplastic films were prepared by film casting method. The alkali extracted agar
(AEA) and photo bleached agar (PBA) were used as raw material for the
formation of bioplastic film. Sago starch and plasticizer (glycerol) were
incorporated into film-forming solution to increase workability. Sago starch
(6.8 g) was first gelatinized and homogenized in 240 mL distilled water using
overhead stirrer in water bath (± 90 ºC). Agar powder was then added to the
homogenized starch solution. Glycerol was mixed to the film-forming solution
and stirred for 5 minutes. The amount of dissolved components were added based
on the formulation from Wu et al. [18] to ensure the surface of bioplastic film
produced is clear, smooth, flexible and without any phase separation. Then, the
film forming solution was casted on petri dish and dried at 50 ºC overnight.

2.4. Characterisation of bioplastic film

2.4.1. FTIR spectroscopy
The spectra of each casted film sample were recorded using FTIR spectrometer
(Thermo Scientific Nicolet iS10). Each spectrum was the average of 32
scans acquired at 2 cm-1 resolution. Transmittance mode was used to determine
peak base lines and heights. The peak heights were calculated and converted
to absorbance.

2.4.2. Scanning electron microscopy (SEM)

Scanning electron microscope (Hitachi S3400N, Japan) with energy dispersive X-
ray analysis (EDX) at 20 kV was used to obtain scanning electron micrographs
and elemental composition of the bioplastic film samples. The bioplastic film
samples were coated with Au/Pd up to 7 nm thick with high resolution sputtering
instrument at a sputtering rate of 1.5 kV per minute. Then, cross sectional image
of the samples were observed using x1000 and x5000 magnification.

2.4.3. Mechanical properties

Tensile strength (TS, MPa) and elongation at break (%) of the bioplastic film
samples were determined using tensile machine (Tinius Olsen H10KS Universal,
USA). The samples were cut into dumb-bell shape and fixed to the tensile
machine at both ends prior to the mechanical testing.

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 Agar from Malaysian Red Seaweed as Potential Material for Synthesis of . . . . 5

2.4.4. Thermogravimetric analysis (TGA)

TGA was carried out using a thermogravimetric analyser (Mettler Toledo TGA-
SD815a, Switzerland). The bioplastic film samples were cut into pieces (2.5 to
5 mg) and transferred to the sample holder. The testing temperature was from 30
to 800 ºC, with a ramp temperature of 20 ºC and nitrogen gas flow rate at
30 mL/min. Curves of weight loss of bioplastic film samples against temperature
were plotted.

2.4.5. Rheological study

Constant shear rate test and dynamic oscillation test were conducted on AEA and
PBA films using rheometer (Anton Paar GmBH-MCR 301, Austria) equipped
with a parallel plate with diameter of 0.5 mm and gap of 1 mm. Each sample of
bioplastic film was placed onto the bottom plate which equilibrated to 25 ºC and
pressed by the top plate. Constant shear rate test was carried out with shear rate at
0.01 s-1 for duration of 477 s. Dynamic oscillation test was conducted in strain
sweeping mode where strain amplitude was changed from 0.01 % to 100 % with
the shear frequency of 1 Hz. G’ (measure of elastic response) and G” (measure of
viscous response) were monitored along the sweep amplitude test.

2.4.6. Soil burial test

Biodegradability of the bioplastic films were examined with soil burial test. The
samples were buried in locations with different type of soils and left for 30 days.
The initial weight (Mo) and the final weight (M1) were recorded. The percentage
of weight loss after 30 days is calculated by using Equation 3.
Mo – M 1
Weight loss (%) = × 100 % (2)
Mo

3. Results and Discussions

3.1. Physical appearance and yield of extracted agar
In this study, the alkali extracted agar (AEA) was in watery-soft gel-like jelly
which clumped together in the solution. Generally, agar from Gracilaria species
is with lower quality because of their high sulphate concentrations [19].
Alkali treatment was used to improve the gel properties by desulphate the native
agar and allow the L-galactose-6-sulfate moieties in agar backbone to convert to
3,6-anhydro-L-galactose and hence increase the gel strength of the extract [20].
The gelling behaviour of AEA in this study indicated that pre-treatment of
seaweed using alkaline solution help to remove the sulphate groups such as 4-O-
methyl-α-L-galactose and D-galactose-6-sulphate residues, thus improving the
gelling ability [17].
As for the photo bleached agar, the colour of seaweed turned from yellow-
green (after alkali pretreatment) to pale yellowish after photo-bleaching process.
The coloured organic matters which are normally found in Gracilaria species are
phycoerythrobilin, chlorophyll α and carotenoids. They are conjugated-double-

Journal of Engineering Science and Technology Special Issue 7/2016

6 S. L. Hii et al.

bond chromogenic group and vulnerable to photochemical degradation. By

exposing the seaweed to solar radiation, coloured organic matters are mineralised
into carbon monoxide, carbon dioxide ammonium and other forms of dissolved
organic carbon [21]. According to Li et al. [11], photo bleaching process helps to
increase the total organic carbon of seaweed. In addition, the authors discovered
that sulphate in the seaweed can be further eliminated by photolysis process. The
sulphate content of seaweed decreased with the increase of gel strength which
may be due to the reaction of sulphate with free radicals.
The yield of AEA (10.68 % ± 0.08) was slightly higher than the yield of PBA
(9.83 % ± 0.03). The agar yield from G. salicornia from both extraction methods
in the present study are lower than those reported from various Gracilaria species
[20, 22, 23]. Extraction temperature is one of the most important factors affecting
the agar yield. Souza et al. [24] reported that higher yield of agar can be obtained
with higher temperature. Other factors affecting the final agar yield are extraction
time, environmental conditions, seasonal variations and physiological factors
[25]. The low agar yield from present study might be caused by leaching of agar
during the thawing-recovery process. Some agar might also lost during the
washing and pre-treatment process [26].

3.2. Morphological properties of bioplastic film

Massive amount of air bubbles trapped inside the bioplastic film formed using
AEA (Fig. 1a) as compared to PBA (Fig. 1b). The surface of the AEA film is dry
and uneven. Although the films are with air bubbles, but in general they are still
rigid, soft and flexible. In an attempt to eliminate the air bubble trapped inside
both of the bioplastic films, the hot film forming solution was degassed using a
sonicator unit.

(a) AEA bioplastic film (b) PBA bioplastic film

Fig. 1. Physical appearance of bioplastic film.

SEM images of AEA and PBA bioplastic films were shown in Fig. 2. Both
AEA and PBA bioplastic films were with air bubbles trapped in the film matrix,
causing the structures for both of the films less dense and not homogeneous.
Nonetheless, there was presence of continuous phase structure found in both

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 Agar from Malaysian Red Seaweed as Potential Material for Synthesis of . . . . 7

films, indicating that the added glycerol was successfully incorporated into the
polysaccharide chain interior and disrupted the intramolecular hydrogen bonds
[27]. It was noticeable that more air bubbles were present in the AEA bioplastic
film than PBA bioplastic film which will weaken the mechanical properties of
AEA bioplastic film.

(a) AEA bioplastic film (b) PBA bioplastic film

Fig. 2. SEM images of bioplastic films.

3.3. FTIR spectroscopy of bioplastic film

Figures 3 show the FTIR analysis of the AEA (Fig. 3a) and PBA film samples
(Fig. 3b). FTIR absorbance bands of AEA and PBA bioplastic films at 3264.56
cm-1 and 3274.23 cm-1 is contributed to the complex vibrational stretches
associated with intra, inter and free molecular hydroxyl group. The FTIR
absorbance bands at 2929.21 cm-1 and 2927.24 cm-1 indicated the hydrogen atom
stretches with the carbon on the methane ring. Stretching molecules of conjugated
peptide bond by acetone and amine groups in both the bioplastic film is indicated
with the presence of absorbance bands at 1643.91 cm-1 and 1644.33 cm-1 [18]. The
properties of ester sulphate in both AEA and PBA bioplastic films is shown in the
FTIR absorbance bands at 1366.14 cm-1 and 1366.06 cm-1. FTIR absorbance
bands of AEA and PBA bioplastic film range from 926.75 cm-1 to 1150.15 cm-1,
and 926.80 cm-1 to 1149.65 cm-1, indicates the C-O bond stretching on anhydro-
galactose ring, which is common in the structure of agar.
Any movement of FTIR spectra absorbance bands due to the addition of
different component indicates distinctive chemical interaction occurred. Ma et al.
[28] mentioned that the FTIR spectra with lower absorbance bands of similar
component might be due to the intense interaction in the material. More intense
chemical interaction is feasible as this indicates a better entanglement between
agar, sago starch and glycerol.
By analysing Fig. 3, the chemical interactions between agar, sago starch and
glycerol were found stronger in PBA bioplastic film as compared to AEA
bioplastic film. This is proven by lower absorbance bands for most of the peaks of
PBA bioplastic film spectrum.

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8 S. L. Hii et al.

(a)

(b)

Fig. 3. FTIR spectrum of bioplastic film:

(a) AEA bioplastic film, (b) PBA bioplastic film.

3.4. Mechanical properties of bioplastic film

Table 1 presents the tensile strength and percent elongation of both AEA and
PBA bioplastic films. PBA bioplastic film exhibited higher tensile strength and
percent elongation than AEA bioplastic film, which further confirmed the
stronger chemical interactions between agar, sago starch and glycerol in PBA
bioplastic film.
There are several other factors affecting the difference in tensile strength and
percent elongation value of plastic film such as the molecular weight,
conformations, chemical structures and hydration behaviour. Temperature and
relative humidity greatly affect the tensile strength and percent elongation of
bioplastic film [29].
In the present study, the production of bioplastic films was with the addition
of glycerol in the composites, which allowed the glycerol to enter the interior
polysaccharides chain of the starch and agar. By this, the inter- or intra-molecular

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 Agar from Malaysian Red Seaweed as Potential Material for Synthesis of . . . . 9

hydrogen bonds were disrupted and plasticized films can be created in a

continuous phase structure [27].

Table 1. Tensile strength and percent elongation of bioplastic film.

Bioplastic Film Tensile Strength (MPa) Elongation (%)
AEA 2.431 2.476
PBA 3.067 3.270

3.5. Thermogravimetric analysis (TGA) of bioplastic film

Thermogravimetric analysis was conducted to determine the effects of thermal
stability of bioplastic films produced in the present study (Table 2). Both of the
bioplastic films experienced weight loss in two distinctive steps. The first weight-
loss step occurred at temperature 187.91 ˚C and 191.76 ˚C for AEA bioplastic
film (10.40 %) and PBA bioplastic film (9.19 %), respectively. The differences in
temperature and weight loss were small. The first step of weight loss was mainly
caused by the evaporation of moisture content of the bioplastic films and glycerol
is known to be a hydrophilic substance that holds moisture [30].

Table 2. TGA of bioplastic film in nitrogen environment.

Bioplastic Film Temperature Weight Loss (%) Residual Weight
Range (ºC) (%)
AEA 26 to 188 10.40 89.61
188 to 466 74.84 14.80
PBA 26 to 192 9.19 90.80
192 to 506 80.52 10.27
At second distinctive step, major weight loss occurred at 465.24 ºC and
505.67 ºC for AEA and PBA bioplastic film, respectively. PBA bioplastic film
contributed to a higher percentage of weight loss at 80.52 % compared to AEA
bioplastic film with weight loss of 74.84 %, respectively at higher temperature.
This phenomenon might be due to the thermal decomposition of components that
made up the bioplastic film. Glycerol decomposed at temperature around 260 ºC
whereas the thermal decomposition of agar and starch are at temperature around
300 ºC [31].
From the thermogravimetric curve for both bioplastic films (Fig. 4), the steep
weight loss (degradation temperature) for AEA bioplastic film was around 300 ºC
and for PBA bioplastic film, around 285 ˚C. The weight loss rate slowly reduced
after 350 ºC which further reconfirmed that most of the components in the
bioplastic film had been decomposed within the reasonable range (thermal
decomposition, glycerol = 260 ºC, agar and starch = 300 ºC). AEA bioplastic film
showed higher thermal stability (14.80 %) as compared to PBA bioplastic film
(10.27 %). Although PBA bioplastic film has better tensile strength and
elongation compared to AEA bioplastic film, it does not attribute to better thermal
stability. Thermal stability of bioplastic film depends on many factors such as the
ability of the molecule to discharge cation and network structure of molecules.
For example, higher degree of cross-linking in a certain composite contributed to
stronger thermal stability [32].

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10 S. L. Hii et al.

Fig. 4. Thermogravimetric curve of bioplastic film:

(a) AEA bioplastic film, (b) PBA bioplastic film.

3.6. Rheological properties of bioplastic film

The constant shear rate tests indicated that both of the bioplastic films are with
non-Newtonian and shear thickening behaviour. This is because the ratio of shear
stress to shear rate of the films were not constant and the viscosity varies with
different shear stress [33]. Moreover, with increasing of shear stress, the viscosity
for both of the films raised tremendously from 145 Ps.s at 1.48 Pa to 7740 Ps.s at
77.4 Pa (AEA bioplastic film) and from 102 Ps.s at 1.03 Pa to 2550 Ps.s at
25.5 Pa (PBA bioplastic film).
Figure 5 shows the amplitude sweep test which was conducted by applying
the amplitude of deformation or amplitude of shear stress in an increasing order
while maintaining constant frequency. From the strain region of 0.1 to 1 %, the G’
and G” for both of the films were in a rather constant position, indicating that
structure breakdown rate for both of the bioplastic blends were same as the
structure rebuilding rate.
When the magnitude of strain proceed into the region of non-linear viscosity
(strain region of 10 to 100 %), the modulus G’ start to decrease all the way till
the end while the modulus G” start to increase before decrease towards the end

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 Agar from Malaysian Red Seaweed as Potential Material for Synthesis of . . . . 11

of the process. This is phenomena of strain overshoot when the structure re-
arranges and thus provides resistance to large strain amplitude, which increase
the value of G”. The re-arranged structure is not able to withstand when the
strain continues to increase. Thus, causing it to breakdown and the decrease of
G” modulus at the end of the process. According to Hyun et al. [34], this type of
shear flow behaviour is also known as the weak strain overshoot. Therefore, the
bioplastic blends of AEA and PBA could be classified as weak gel system.
Weak gels contain crosslink which is able to flow without fracture and have the
ability to reform [35].

Fig. 5. Graph of G’ and G” of AEA bioplastic blend (■)

and PBA bioplastic blend (●) against the strain.

3.7. Soil burial test

Soil burial test was carried out to determine the biodegradability of AEA and
PBA bioplastic films in different type of soil condition. Biodegradability is
known as the ability of polymer undergoes structural alteration and the scission of
polymeric chains that affects by environmental factors and microorganism
activities [36]. The percentage of weight loss of PBA bioplastic film was lower
than AEA bioplastic film (Table 3). This might be due to the packed structure of
PBA bioplastic film compared to AEA bioplastic film. More air bubbles were
trapped in AEA bioplastic film as seen in the SEM image. Hence, the decreased
in the dense structure of poly matrix of AEA bioplastic film and increased in the
water absorption capability that promotes the growth of microorganisms.
Based on the results from the present study (Table 3), AEA bioplastic film
was able to fully decompose after 30 days while for PBA bioplastic film, it was
half decomposed. It was found that highest weight loss for bioplastic film was
achieved at the burial location that was damp and shaded from sunlight although
all the burial locations were having similar pH range of 6.9 to 8.3.

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12 S. L. Hii et al.

Agar is known as sulphated polysaccharide which contains charged groups in

their extended chains with properties of high hydrophilicity. Therefore, the
presence of different extracted agar increased the hygroscopic characteristics of
the bioplastic films and promotes the growth of microorganisms and hence the
weight loss of the films. Besides, composite bioplastic films that contained starch
speed up the degradation where it improved the porosity and also provided food
source for the growth of microorganism. The promotion of the microorganism
growth caused the bioplastic films to lose its structural integrity and facilitate the
attack of polymeric matrix [37].
Addition of hydrophilic starch and glycerol in the composition film also
improves the water absorption capability and hence increases the water activity of
films and promotes the microorganism growth. Glycerol from the composite film
passes through the cell membrane and metabolises by the microorganisms also
enhanced the weight loss of bioplastic films [38].

Table 3. Percentage of weight loss of bioplastic film

Location Weight loss (%)
AEA bioplastic PBA bioplastic
film film
Location 1 75.05 25.78
(Dry; exposed to sun, near to
building)
Location 2 (Shaded by 99.29 43.27
building; soil is damp)
Location 3 61.51 36.84
(Dry; exposed to sun; near to
shrubs)

4. Conclusions
In the present study, agar extracted from different extraction technique were rigid,
brittle with uneven surface and have an average yield of 9 to 11 %. FTIR spectra
of AEA and PBA bioplastic films confirmed that there are chemical interactions
between agar, sago starch and glycerol with PBA having the stronger interaction
effects. The SEM images and mechanical tests further revealed the PBA
bioplastic film is with better mechanical properties (higher tensile strength and
percent elongation) which have denser and packed structure. The AEA bioplastic
film exhibited excellent biodegrability with the highest weight loss of 99.29 % in
comparison to PBA bioplastic films with 43.27 % weight loss within 30 days of
soil burial test. TGA results indicated that thermal stability of AEA bioplastic
film is better than PBA bioplastic film. Rheological analysis of the biopolymer
blends showed that all the bioplastic blends produced are non-Newtonian fluid
and thus performed like a weak gel system.
As conclusion, there are a lot of explorations and optimizations to be made to
both of the bioplastic films but there is no doubt that agar extracted from G.
salicornia are able to bring future potential to a wide range of bioplastic
applications in the industry.

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 Agar from Malaysian Red Seaweed as Potential Material for Synthesis of . . . . 13

Acknowledgement
The authors would like to acknowledge the followings: Ministry of Education
Malaysia (ERGS/1/2013/TK04/TAYLORS/02/01), Universiti Tunku Abdul
Rahman (UTAR) and Taylor’s University (TU) for funding and technical support.

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