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    Lab - Stoma Opening in Response To Light

    Uploaded by

    Guillaume Erard
    The document outlines a protocol to investigate how light affects the opening of stoma on plant leaves. The protocol involves wrapping half of a plant's leaves in foil the day before to block light, then applying nail polish to leaves that were exposed to light or wrapped in foil. The nail polish peels are observed under a microscope to count the ratio of open to closed stoma and compare between light-exposed and foil-wrapped leaves.

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    Lab - Stoma Opening in Response To Light

    Uploaded by

    Guillaume Erard
    19 views1 page
    The document outlines a protocol to investigate how light affects the opening of stoma on plant leaves. The protocol involves wrapping half of a plant's leaves in foil the day before to block light, then applying nail polish to leaves that were exposed to light or wrapped in foil. The nail polish peels are observed under a microscope to count the ratio of open to closed stoma and compare between light-exposed and foil-wrapped leaves.

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    Lab - Stoma Opening in Response to Light

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    The document outlines a protocol to investigate how light affects the opening of stoma on plant leaves. The protocol involves wrapping half of a plant's leaves in foil the day before to block light, then applying nail polish to leaves that were exposed to light or wrapped in foil. The nail polish peels are observed under a microscope to count the ratio of open to closed stoma and compare between light-exposed and foil-wrapped leaves.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    19 views1 page

    Lab - Stoma Opening in Response To Light

    Uploaded by

    Guillaume Erard
    The document outlines a protocol to investigate how light affects the opening of stoma on plant leaves. The protocol involves wrapping half of a plant's leaves in foil the day before to block light, then applying nail polish to leaves that were exposed to light or wrapped in foil. The nail polish peels are observed under a microscope to count the ratio of open to closed stoma and compare between light-exposed and foil-wrapped leaves.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    Stoma opening in response to light

    Aim: Investigate the effect of light on the opening of the plant leaf stoma.

    Protocol:
    1. The day before, wrap half of a plant's leaves with tin foil
    2. On the day of the experiment, make sure that the plant has been exposed to light for at least 2 h
    3. Remove and unwrap the leaves
    4. Immediately paint a 1 cm3 patch of clear nail varnish on each leaf
    5. Label each leaf as light or dark and let the varnish dry
    6. Apply clear tape on the varnish and peel off
    7. Stick the tape and varnish peel on a microscope slide
    8. Observe and count the ratio of open/closed stoma in each prep (count at least 20 stoma per sample)
    9. Make sure you observe at least 3 sample for light and dark
    10. Record your results and process accordingly (table and graph)

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