CL (ppm) (fi-fo)

6.48 0.2 x 10-2

5.76 0.6 x 10-2
5.2 1.0 x 10-2 OB Method
2.4 2.2 x 10 -2
7
f(x) = − 1.512 x + 8.624
0.6 4.2 x 10-2 R² = 0.922975556818769
6

ρ 0.0012 5
Air flow ra 0.5 vvm
4
V 0.91
Q 0.455 3
Slope -1.512
Kla (-ρ*Q/Slope*V) 2

0.000546 1
-1.37592
Kla 0.000399 0
0.5 1 1.5 2 2.5 3 3.5 4
C* 8.624

rCx at any ρ*Q/V *(fi-fo)

 OB Method

69

2.5 3 3.5 4 4.5 5 5.5

 Rotation speed of HRF, rpm
Fermenta
50 100 120
tion
C L- %O2 in %O2 in %O2 in
Time (hrs) CL- ppm CL- ppm
ppm exit exit exit
0 5.9 21 5.9 21 5.9 21
2 5.5 20.9 5.7 20.9 5.8 20.9
4 4.9 20.8 5.2 20.8 5.4 20.7
6 4 20.5 4.3 20.4 4.4 20.4
8 2.5 20.1 2.5 19.8 2.8 19.5
9 1.4 19.8 1.7 19.2 2 19
12 2.4 20.2 2 19.5 2.1 19.3

50 RPM 100 RPM 150 RPM

fi = 21
CL (ppm) fi - fo CL (ppm) fi - fo CL (ppm) fi - fo
5.9 0 5.9 0 5.9 0
5.5 0.1 5.7 0.1 5.8 0.1
4.9 0.2 5.2 0.2 5.4 0.3
4 0.5 4.3 0.6 4.4 0.6
2.5 0.9 2.5 1.2 2.8 1.5
1.4 1.2 1.7 1.8 2 1
2.4 0.8 2 1.5 2.1 1.7

No information is provided about density of air, flow rate of air, working volume.
 50 RPM
7

6
f(x) = − 3.79166666666667 x + 5.80416666666667
5 R² = 0.988028953229399

0
0 0.2 0.4 0.6 0.8 1 1.2 1.4

100 RPM
7

6
f(x) = − 2.46039603960396 x + 5.7980198019802
5 R² = 0.985420157400355

0
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2

150 RPM
7

6
f(x) = − 2.37004175365345 x + 5.81774530271399
5 R² = 0.854291125840633

2
6
f(x) = − 2.37004175365345 x + 5.81774530271399
5 R² = 0.854291125840633

0
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8
Time (s) CL(ppm) Degassing period Gassing period
0 5.2 Time (s) CL(ppm) Time (s) CL(ppm)
10 5.2 30 5.2 110 0.9
20 5.2 40 4.7 120 2.8
30 5.2 50 4.2 130 3.8
40 4.7 60 3.7 140 4.2
50 4.2 70 3.1 150 4.4
60 3.7 80 2.6 160 4.5
70 3.1 90 2
80 2.6 100 1.5
90 2 rCX = 0.0532
100 1.5
110 0.9
120 2.8
130 3.8
140 4.2 0
150 4.4 0 RCX
160 4.5 0
6

5 f(x) = − 0.0532142857142857 x + 6.83571428571429

R² = 0.998965082793377
4

0
20 30 40 50 60 70 80 90 100
 Time (s) CL dCL/dt dCL/dt + rCX
0 5.2 0 0.0532
10 5.2 0 0.0532
20 5.2 0 0.0532
30 5.2 0 0.0532
40 4.7 -0.05 0.0032
50 4.2 -0.05 0.0032
60 3.7 -0.05 0.0032
70 3.1 -0.06 -0.0068
80 2.6 -0.05 0.0032
90 2 -0.06 -0.0068
100 1.5 -0.05 0.0032
110 0.9 -0.06 -0.0068
120 2.8 0.19 0.2432
130 3.8 0.1 0.1532
140 4.2 0.04 0.0932
150 4.4 0.02 0.0732
160 4.5 0.01 0.0632
Kla = 0.108 s^1
C* = 5.0942

KLA
5
4.5
f(x) = − 9.21903052064632 x + 5.09422262118492
4 R² = 0.990370747353951
3.5
3
90 100 2.5
2
1.5
1
0.5
0
0.05 0.1 0.15 0.2 0.25 0.3
 Air off Air on

Time (secs) DO Time (secs) DO

cove (ppm) cove (ppm)
10 3.7 50 0.15 Degassing period
15 3.7 55 1.7 Time (s) DO
20 3.7 60 2.41 30 3.7
25 3.7 65 2.9 35 3.2
30 3.7 70 3.2 40 1.8
35 3.2 75 3.3 45 0.8
40 1.8 80 3.41 rCX = 0.202
45 0.8 85 3.52 B' = 3.61 (Numerical value is the equlibrium concentration under aerate
90 3.59
95 3.61
100
RCX
4
f(x) = − 0.202 x + 9.95
3.5 R² = 0.971986660314436
3

2.5

1.5

0.5

0
28 30 32 34 36 38 40 42 44
 Time (s) C*-CLO/C*LN(C*-CLO/C*-CL)
3.55 50 1 0 4

55 1.775 0.5738 3.5 f(x) = 0.0805957972887

R² = 0.99240071399922
60 2.751938 1.012305
3
65 4.4375 1.490091
70 7.1 1.960095 2.5

75 8.875 2.183238 2
m concentration under aerated conditions) 80 12.24138 2.504822 1.5
85 19.72222 2.981746
1
90 32.27273 3.474223
95 39.44444 3.674893 0.5
X 0
Kla 0.0806 40 50 60

Time 1 -CL/alph Ln
50 0.958449 -0.042439
55 0.529086 -0.636605 K
60 0.33241 -1.101386 0
65 0.196676 -1.626198 45 50 f(x) =55− 0.113968415137115
60 65 x +
R² = 0.955953634265872
70 0.113573 -2.175306 -1
75 0.085873 -2.454891
80 0.055402 -2.893146 -2
85 0.024931 -3.691653
38 40 42 44 46
90 0.00554 -5.195731 -3
95 0 Err:502
-4

-5

-6
 KLa

f(x) = 0.0805957972887325 x − 3.85767392321953

R² = 0.992400713999222

50 60 70 80 90 100

Kla
50 f(x) =55− 0.113968415137115
60 65 x + 70 75 80
5.77586078188378 85 90 95
R² = 0.955953634265872