Scribd Logo
Upload

Welcome to Scribd!

  • 50 views

    6 Practical Microtechnique

    Uploaded by

    tube71393
    The document provides instructions for microtechnique procedures in molecular biology, including: 1. Preparing a paraffin block by embedding a tissue sample within a block cavity created by L-shaped metal pieces arranged on a glass plate and filled with molten paraffin wax. 2. Trimming excess wax from the solidified paraffin block to obtain the proper shape and size for mounting. 3. Mounting the trimmed paraffin block on a microtome metallic peg by applying molten wax to attach the block centrally and filling gaps to secure it before section cutting.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    6 Practical Microtechnique

    Uploaded by

    tube71393
    50 views2 pages
    The document provides instructions for microtechnique procedures in molecular biology, including: 1. Preparing a paraffin block by embedding a tissue sample within a block cavity created by L-shaped metal pieces arranged on a glass plate and filled with molten paraffin wax. 2. Trimming excess wax from the solidified paraffin block to obtain the proper shape and size for mounting. 3. Mounting the trimmed paraffin block on a microtome metallic peg by applying molten wax to attach the block centrally and filling gaps to secure it before section cutting.

    Original Description:

    Cuhu

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document provides instructions for microtechnique procedures in molecular biology, including: 1. Preparing a paraffin block by embedding a tissue sample within a block cavity created by L-shaped metal pieces arranged on a glass plate and filled with molten paraffin wax. 2. Trimming excess wax from the solidified paraffin block to obtain the proper shape and size for mounting. 3. Mounting the trimmed paraffin block on a microtome metallic peg by applying molten wax to attach the block centrally and filling gaps to secure it before section cutting.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    50 views2 pages

    6 Practical Microtechnique

    Uploaded by

    tube71393
    The document provides instructions for microtechnique procedures in molecular biology, including: 1. Preparing a paraffin block by embedding a tissue sample within a block cavity created by L-shaped metal pieces arranged on a glass plate and filled with molten paraffin wax. 2. Trimming excess wax from the solidified paraffin block to obtain the proper shape and size for mounting. 3. Mounting the trimmed paraffin block on a microtome metallic peg by applying molten wax to attach the block centrally and filling gaps to secure it before section cutting.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    B.Sc.

    III Sem VI : ZOOLOGY PRACTICAL


    MICROTECHNIQUE AND MOLECULAR BIOLOGY
    -----------------------------------------------------------------------------------
    MICROTECHNIQUES :

    6. Practical:

    Microtechnique: 2
    (Preparation of paraffin block, Trimming and mounting of block)
    In micro technique after proper embedding of tissue, preparation of block making
    are followed

    5. Preparation of paraffin block

    Paraffin block are prepared with the help of metallic ‘L’ shaped pieces. Take a clean
    glass plate and apply glycerin on the surface of it. Take the L pieces, apply glycerin
    on it and arrange it on glass plate with rectangular or square shape . Add molten
    paraffin wax in the cavity of L pieces and allow it to solidify except its upper
    surface . To avoid upper surface solidification apply warm needle or forcep over the
    surface and melt the crust. After proper solidification take a tissue and keep it in a
    block cavity. Arrange it as per requirement and allow it to complete solidification.
    After complete solidification glass plate along with paraffin block transfer it into
    water tray.
    6. Trimming of paraffin
    araffin bloc
    block

    Removal off excess wax from the block for proper


    roper shape is calle
    called as trimming.
    According to the requirement of shape and size
    ize excess wax is removed from the
    block by rubbing
    bbing over plane su
    surface. Clean the edges and surface of
    o the block after
    trimming, paraffin block is read
    ready for mounting.

    7. Mounting of block

    For section cutting paraffin block


    blo are mount on microtome metallic peg. Microtome
    metallic pegg is a rough circular disc having small
    all shaft. Apply molten
    molt wax over the
    rough surface
    ace ofcircular disc. After
    A thick layer of wax make a central
    centr cavity on the
    wax surfacee and fix the block into
    i it . Fill up the
    he gaps with molten wax and seal it.
    After mounting
    nting apply thin layer of bees wax on
    n opposite surface of the block. After
    applying bee
    ee wax block is ready for section cutting.

    Microtome metallic peg

    You might also like