Scribd Logo
Upload

Welcome to Scribd!

  • 58 views

    Gentech TP1100

    Uploaded by

    yuli perea
    The document provides information about ExcelTaq 5X PCR Master Mix, a ready-to-use mixture for PCR that contains Taq DNA polymerase, reaction buffer, MgCl2, dNTPs, and stabilizers. It can be used for a variety of PCR applications involving amplification of DNA fragments up to 8 kb. The master mix yields high-quality PCR products and reduces errors from pipetting. It is supplied along with 6X DNA loading dye for post-PCR gel electrophoresis analysis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Gentech TP1100

    Uploaded by

    yuli perea
    58 views8 pages
    The document provides information about ExcelTaq 5X PCR Master Mix, a ready-to-use mixture for PCR that contains Taq DNA polymerase, reaction buffer, MgCl2, dNTPs, and stabilizers. It can be used for a variety of PCR applications involving amplification of DNA fragments up to 8 kb. The master mix yields high-quality PCR products and reduces errors from pipetting. It is supplied along with 6X DNA loading dye for post-PCR gel electrophoresis analysis.

    Original Description:

    Gentech

    Original Title

    gentech-TP1100

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document provides information about ExcelTaq 5X PCR Master Mix, a ready-to-use mixture for PCR that contains Taq DNA polymerase, reaction buffer, MgCl2, dNTPs, and stabilizers. It can be used for a variety of PCR applications involving amplification of DNA fragments up to 8 kb. The master mix yields high-quality PCR products and reduces errors from pipetting. It is supplied along with 6X DNA loading dye for post-PCR gel electrophoresis analysis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    58 views8 pages

    Gentech TP1100

    Uploaded by

    yuli perea
    The document provides information about ExcelTaq 5X PCR Master Mix, a ready-to-use mixture for PCR that contains Taq DNA polymerase, reaction buffer, MgCl2, dNTPs, and stabilizers. It can be used for a variety of PCR applications involving amplification of DNA fragments up to 8 kb. The master mix yields high-quality PCR products and reduces errors from pipetting. It is supplied along with 6X DNA loading dye for post-PCR gel electrophoresis analysis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 8

    www.smobio.

    com

    Product information ExcelTaq™ series


    5X PCR Master Mix

    TP1100 200 RXN


    5X PCR Master Mix 1 ml × 2
    6X DNA Loading Dye (Blue) 1 ml × 2

    Storage
    4°C for 6 months
    -20°C for 24 months

    Caution: Avoid Multiple Freeze/Thaw Cycles

    P06-F11-A1
    Description
    The ExcelTaq™ 5X PCR Master Mix is a ready-to-use
    mixture for amplifying targeted DNA fragments. It is
    designed to serve as a master mix for virtually all PCR
    applications. The mixture contains all components for
    PCR with the exception of templates and primers. This
    not only saves valuable time in the laboratory, but
    also reduces pipetting and reagent handling errors.
    The PCR Master Mix is supplied as a 5X concentrated
    ready-to-use mixture of recombinant Taq DNA
    Polymerase, reaction buffer, MgCl2, dNTP, and
    enzyme stabilizer enabling efficient amplification of
    template in PCR and allows the user to prepare a PCR
    reagent conveniently. This product is supplied with 6X
    DNA Loading Dye (Blue) containing two tracking dyes
    (Xylene cyanol FF and Bromophenol blue) for post
    PCR analysis through the use of agarose gel
    electrophoresis.

    P06-F11-A1
    Features
     5’→3’ DNA polymerase activity
     No detectable 3'→5' exonuclease (proofreading)
    activity
     Generates PCR products with 3'-dA overhangs
     High yield PCR
     High reproducibility
     Reduced pipetting errors

    Applications
     Routine PCR
     Colony PCR
     High throughput PCR
     Amplification of DNA fragments up to 8 kb
     Generation of PCR products for TA cloning
     DNA labeling

    P06-F11-A1
    Recommended PCR Condition
    Template 1 – 150 ng
    Forward primer 0.1 – 0.5 µM
    Reverse primer 0.1 – 0.5 µM
    5X PCR Master Mix 10 µl
    ddH2O to 50 µl
    Total volume 50 µl

    Recommended PCR Program


    94°C 2 min
    94°C 30 sec
    50~68°C* 30 sec 25 ~ 40 cycles
    72°C 30 sec/kb
    72°C 1 min

    *Optimal PCR condition varies according to primers’ thermodynamic


    properties.

    P06-F11-A1
    Quality Control

    Functional Testing
    ExcelTaq™ 5X PCR Master Mix is tested for performance
    in the polymerase chain reaction (PCR) in a 50 µl
    standard reaction condition to amplify a 665 bp gene
    from 10 pg of tested plasmid DNA. The resulting PCR
    product is visualized as a single band on an ethidium
    bromide-stained agarose gel.

    Nuclease Assay
    No contaminating endonuclease or exonuclease
    activity was detected using pUC19 incubated with
    ExcelTaq™ 5X PCR Master Mix (1:5 dilution) for 4 hours
    at 37°C.

    Residual Nucleotides Assay


    No contaminating residual nucleotides were detected
    from ExcelTaq™ 5X PCR Master Mix by PCR assay.

    P06-F11-A1
    Other Information
    SMOBIO Technology, Inc. claims all warranties with
    respect to this document, expressed or implied,
    including but not limited to those of merchantability
    or fitness for a particular purpose. In no event shall
    SMOBIO Technology, Inc. be liable, whether in
    contract, tort, warranty, or under any statute or any
    other basis for special, incidental, indirect, punitive,
    multiple or consequential damages in connection
    with or arising from this document, including but not
    limited to the use thereof.

    Caution: Not intended for human or animal diagnostic or therapeutic


    uses.

    P06-F11-A1
    Related Products
    CK1000 Champion E. coli Transformation Kit
    CV1100 GetClone PCR Cloning Vector II, 20 RXN
    DM1100 ExcelBand 50 bp DNA Ladder, 500 µl
    DM2100 ExcelBand 100 bp DNA Ladder, 500 µl
    DM2300 ExcelBand 100 bp+3K DNA Ladder, 500 µl
    DM3100 ExcelBand 1 KB (0.25-10 kb) DNA Ladder,
    500 µl
    NS1000 FluoroVue Nucleic Acid Gel Stain
    (10,000X), 500 μl
    RP1000 ExcelRT Reverse Transcriptase, 20,000 U
    TF1000 SMO-HiFi DNA Polymerase, 100 U
    TF3000 G-HiFi DNA Polymerase, 100 U
    TP1000 ExcelTaq DNA Polymerase, 500 U
    TP1200 ExcelTaq 5× PCR Master Dye Mix, 200 RXN
    TP1260 ExcelTaq 5× Fluorescent PCR Master Mix,
    200 RXN
    TP2100 ExcelTaq Blood Direct PCR Master Mix Kit,
    200 RXN
    TP5000 ExcelTaq Hot Start II DNA Polymerase, 500 U
    TQ1110 ExcelTaq 2× Q-PCR Master Mix (SYBR,
    ROX), 200 RXN
    VE0100 B-BOX Blue Light LED epi-illuminator, AC
    100-240V, 50/60Hz

    P06-F11-A1
    B-BOXTM Blue Light LED epi-illuminator

    For research use only


    2019 ver. 2.2.1

    P06-F11-A1

    You might also like