Genome Engineering Technologies
Genome Engineering Technologies
Genome Engineering Technologies
EDITING
TECNOLOGIES
SHIVANSH MANOJ
2021BB10334
INTRODUCTION
In today's rapidly advancing scientific landscape,
genome editing technologies stand at the
forefront of groundbreaking innovations.
TALENS
ZFNS
(ZINC FINGER NUCLEASES)
(TRANSCRIPTION ACTIVATOR-LIKE CRISPR-CAS9:
EFFECTOR NUCLEASES)
Developed in the early 2000s. Emerged around 2010. First described in the mid-2010s.
Use engineered proteins (zinc Use TALE proteins to guide Utilizes RNA-guided Cas9
finger proteins) to target nuclease enzymes to precise proteins to target and modify
specific DNA sequences. DNA locations. DNA.
Pioneered the concept of Enhanced targeting Simplicity, precision, and
programmable DNA cleavage. capabilities. versatility made CRISPR-Cas9 a
revolutionary leap in the field.
THE CRISPR-CAS9 SYSTEM
The CRISPR-Cas9 system is a game-changing genome editing technology
that has gained widespread attention and acclaim in the scientific
community. In this slide, we will explore how CRISPR-Cas9 works and its key
applications in fields such as gene therapy and agriculture.
2. CAS9 PROTEIN
3. ADAPTATION
6. DNA CLEAVAGE
9. FUNCTIONAL OUTCOMES
APPLICATIONS IN HUMAN HEALTH
Ethical Considerations
CRISPR's power raises ethical concerns.
Debate over misuse, designer babies, and germline editing.
Vital ethical guidelines for responsible use and individual rights.
Advancements in Pharmaceuticals
Gene editing in the environment demands robust regulations.
Oversight, containment, and misuse prevention are critical.
Ethical issues revolve around modified organisms and ecosystem
alterations.
CHALLENGES AND ETHICAL CONCERNS
Off-Target Effects
Unintended gene alterations from genome editing.
Potential for unexpected consequences.
Need for improved precision and safety measures.
Germline Editing
Heritable DNA changes, impacting future generations.
Raises profound ethical and safety concerns.
Requires strict international regulations.
Off-Target Effects
Unintended gene alterations from genome editing.
Potential for unexpected consequences.
Need for improved precision and safety measures.
Germline Editing
Heritable DNA changes, impacting future generations.
Raises profound ethical and safety concerns.
Requires strict international regulations.
Case Studies
Examine real-world cases to show the complexity of decision-making.
Gene therapy, agriculture, and environmental examples.
Highlight the outcomes of responsible genome editing.
Public Perception
Public views influence genome editing's acceptance.
Address concerns, maintain transparency, and build trust.
Engage with the public and stakeholders to align with ethical
standards.
CONCLUSION
1. Genome Editing's Revolution: Genome editing technologies, such as CRISPR-Cas9, have revolutionized the
fields of science, medicine, agriculture, and biotechnology. Their precision and versatility offer
unprecedented opportunities.
2. Applications with Impact: Genome editing is instrumental in gene therapy, disease treatment, sustainable
agriculture, and bio-based materials. It addresses critical global challenges.
3. Ethical Considerations: The power of genome editing comes with significant ethical concerns, including
germline editing and the responsible use of technology.
4. Environmental Implications: Genome editing has the potential to enhance conservation efforts, reduce
environmental impact, and tackle climate change.
5. Challenges and Risks: Unintended off-target effects, germline editing, and ethical concerns pose challenges
that demand careful navigation and strict regulations.
6. Balancing Act: Weighing benefits against risks is a complex task. Case studies reveal the intricacies of
decision-making in gene editing applications.
7. Public Engagement: Public perception and engagement play a significant role in shaping the acceptance
and ethical use of genome editing technologies.
8. The Road Ahead: As we move forward, responsible, transparent, and ethical genome editing
practices are pivotal in harnessing the technology's potential for the betterment of humanity and
the environment.
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