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    IFU Egg Yolk Tellurite Emulsion

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    okta
    The document describes an egg yolk tellurite emulsion supplement used to detect coagulase-positive staphylococci according to ISO 6888. The supplement contains egg yolks and potassium tellurite in a saline solution and is used with Baird Parker Agar Base to isolate and enumerate Staphylococcus aureus in foods.

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    © All Rights Reserved
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    IFU Egg Yolk Tellurite Emulsion

    Uploaded by

    okta
    20 views4 pages
    The document describes an egg yolk tellurite emulsion supplement used to detect coagulase-positive staphylococci according to ISO 6888. The supplement contains egg yolks and potassium tellurite in a saline solution and is used with Baird Parker Agar Base to isolate and enumerate Staphylococcus aureus in foods.

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    80122_IFU Egg yolk tellurite emulsion

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    The document describes an egg yolk tellurite emulsion supplement used to detect coagulase-positive staphylococci according to ISO 6888. The supplement contains egg yolks and potassium tellurite in a saline solution and is used with Baird Parker Agar Base to isolate and enumerate Staphylococcus aureus in foods.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    20 views4 pages

    IFU Egg Yolk Tellurite Emulsion

    Uploaded by

    okta
    The document describes an egg yolk tellurite emulsion supplement used to detect coagulase-positive staphylococci according to ISO 6888. The supplement contains egg yolks and potassium tellurite in a saline solution and is used with Baird Parker Agar Base to isolate and enumerate Staphylococcus aureus in foods.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    © Liofilchem® - Egg Yolk Tellurite Emulsion - Rev.0 / 03.10.

    2016
    Instructions For Use
    ENGLISH
    Egg Yolk Tellurite Emulsion
    Supplement for detection of coagulase-positive staphylococci, according to ISO 6888.

    DESCRIPTION
    A sterile, stabilized emulsion of egg yolk containing potassium tellurite for use in Baird Barker Agar Base (ref.
    610004, 620004). The complete medium is used for the isolation and enumeration of Staphylococcus aureus in
    food, in accordance with ISO 6888 (part 1 and 3).

    COMPOSITION
    Chicken egg yolks in an equal volume of saline solution (0.9% NaCl) with potassium tellurite (2 g/l).

    METHOD PRINCIPLE
    Egg yolk is broken down by staphylococci containing lecithinase to yield clear zones around the colonies.
    Potassium tellurite is reduced by coagulase-positive staphylococci causing blackening of colonies.

    PROCEDURE FOR USE


    Aseptically, add 50 ml to 1 litre of Baird Parker Agar Base sterilized in autoclave and cooled to 45-50°C.
    Final concentration of tellurite in medium is 0.01% w/v.

    TECHNIQUE AND INTERPRETATION OF RESULTS


    Refer to the technical sheet of the medium being prepared.

    APPEARANCE
    Yellowish opaque emulsion. May contain a precipitate that can be resuspended.

    STORAGE
    Store at 2-8°C away from light. Do not use the product beyond its expiry date on the label or if product shows
    any evidence of contamination or any sign of deterioration. Mix well before use.

    SHELF LIFE
    1 year.

    QUALITY CONTROL
    Cultural response in Baird Parker Agar after 48 hours incubation at 37 ± 1°C:
    Microorganism Specification
    Staphylococcus aureus WDCM 00034 Good growth, black or grey colonies with clear halo
    Escherichia coli WDCM 00012 Total inhibition
    Staphylococcus saprophyticus WDCM 00159 Black or grey colonies without halo
    Staphylococcus epidermidis WDCM 00036 Black or grey colonies without halo

    WARNING AND PRECAUTIONS


    The product does not contain hazardous substances in concentrations exceeding the limits set by current
    legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety
    data sheet for its correct use. The product is intended for professional use only and must be used by properly
    trained operators.

    DISPOSAL OF WASTE
    Disposal of waste must be carried out according to national and local regulations in force.

    Page 1 of 2
    © Liofilchem® - Egg Yolk Tellurite Emulsion - Rev.0 / 03.10.2016

    BIBLIOGRAPHY
    1. EN ISO 11133:2014. Microbiology of food, animal feed and water − Preparation, production, storage and
    performance testing of culture media.
    2. ISO 6888-3:2003. Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration
    of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 3 : detection and MPN
    technique for low numbers.
    3. ISO 6888-1:1999. Microbiology of food an animal feeding stuffs. Horizontal method for the enumeration of
    coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 1: Technique using Baird
    Parker Agar Medium.
    4. Baird-Parker A.C. (1962) An improved diagnostic and selective medium for isolating coagulase-positive
    staphylococci. J. Appl. Bacteriol. 25:12-19.

    PRESENTATION Contents Ref.


    Egg Yolk Tellurite Emulsion 4 x 50 ml bottles 80122
    Egg Yolk Tellurite Emulsion 6 x 100 ml bottles 80125

    TABLE OF SYMBOLS
    Do not reuse Fragile, handle
    LOT Batch code Manufacturer Use by
    with care

    Temperature Contains sufficient for Caution, consult


    REF Catalogue number
    limitation <n> tests Instruction For Use

    LIOFILCHEM® s.r.l.
    Via Scozia zona ind.le, 64026 Roseto degli Abruzzi (Te) Italy
    Tel. +39 0858930745 Fax +39 0858930330 www.liofilchem.net [email protected]

    Page 2 of 2
    © Liofilchem® - Egg Yolk Tellurite Emulsion - Rev.0 / 03.10.2016
    Istruzioni per l’uso
    ITALIANO
    Egg Yolk Tellurite Emulsion
    Supplemento per la ricerca di stafilococchi coagulasi positivi, secondo ISO 6888.

    DESCRIZIONE
    Un‘emulsione sterile stabilizzata di tuorlo d’uovo contenente tellurito di potassio da utilizzare con Baird Parker
    Agar Base (ref. 610004, 620004). Il terreno completo è utilizzato per l’isolamento ed il conteggio di
    Staphylococcus aureus negli alimenti, secondo ISO 6888 (parte 1 e 3).

    COMPOSIZIONE
    Tuorli d’uova di gallina in un egual volume di soluzione fisiologica (0.9 NaCl) con tellurito di potassio (2 g/l).

    PRINCIPIO DEL METODO


    Il tuorlo d'uovo è degradato dagli stafilococchi produttori di lecitinasi formando un alone chiaro intorno alle
    colonie. Il potassio tellurito è ridotto dagli stafilococchi coagulasi-positivi provocando l'annerimento delle
    colonie.

    PROCEDURA DI UTILIZZO
    In asepsi, aggiungere 50 ml ad 1 litro di Baird Parker Agar Base sterilizzato in autoclave e raffreddato a
    45-50°C.
    La concentrazione finale di tellurito di potassio nel terreno è 0.01% p/v.

    TECNICA ED INTERPRETAZIONE DEI RISULTATI


    Fare riferimento alla scheda tecnica del terreno in preparazione.

    ASPETTO
    Emulsione giallastra opaca. Può contenere un precipitato che può essere risospeso.

    CONSERVAZIONE
    Conservare a 2-8°C al riparo dalla luce. Non usare il prodotto dopo la sua data di scadenza indicata
    sull’etichetta o se il prodotto mostra segni di contaminazione o deterioramento. Miscelare bene prima dell’uso.

    VALIDITÀ
    1 anno.

    CONTROLLO DI QUALITÀ
    Risposte colturali in Baird Parker Agar dopo 48 ore di incubazione a 37 ± 1°C:
    Microrganismo Specifiche
    Staphylococcus aureus WDCM 00034 Crescita buona, colonie nere o grigie con alone chiaro
    Escherichia coli WDCM 00012 Inibizione totale
    Staphylococcus saprophyticus WDCM 00159 Colonie nere o grigie senza alone
    Staphylococcus epidermidis WDCM 00036 Colonie nere o grigie senza alone

    AVVERTENZE E PRECAUZIONI
    Il prodotto non contiene sostanza nocive in concentrazioni superiori ai limiti fissati dall’attuale legislazione e
    perciò non è classificato come pericoloso. Ciononostante si raccomanda di consultare la scheda di sicurezza
    per il suo corretto uso. Il prodotto è da intendersi per in ambito professionale e deve essere utilizzato
    esclusivamente da operatori adeguatamente addestrati.

    SMALTIMENTO DEI RIFIUTI


    Lo smaltimento dei rifiuti deve essere effettuato in conformità alle normative nazionali e locali in vigore.

    Pagina 1di 2
    © Liofilchem® - Egg Yolk Tellurite Emulsion - Rev.0 / 03.10.2016

    BIBLIOGRAFIA
    1. EN ISO 11133:2014. Microbiology of food, animal feed and water − Preparation, production, storage and
    performance testing of culture media.
    2. ISO 6888-3:2003. Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration
    of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 3 : detection and MPN
    technique for low numbers.
    3. ISO 6888-1:1999. Microbiology of food an animal feeding stuffs. Horizontal method for the enumeration of
    coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 1: Technique using Baird
    Parker Agar Medium.
    4. Baird-Parker A.C. (1962) An improved diagnostic and selective medium for isolating coagulase-positive
    staphylococci. J. Appl. Bacteriol. 25:12-19.

    PRESENTAZIONE Contenuto Ref.


    Egg Yolk Tellurite Emulsion Flaconi 4 x 50 ml 80122
    Egg Yolk Tellurite Emulsion Flaconi 6 x 100 ml 80125

    TABELLA DEI SIMBOLI


    Codice del Non riutilizzare Fragile, maneggiare
    LOT lotto
    Fabbricante Utilizzare entro
    con cura

    Numero di Contenuto sufficiente per Attenzione, Consultare


    REF catalogo
    Limiti di temperatura
    <n> saggi le istruzioni per l’uso

    LIOFILCHEM® s.r.l.
    Via Scozia zona ind.le, 64026 Roseto degli Abruzzi (Te) Italy
    Tel. +39 0858930745 Fax +39 0858930330 www.liofilchem.net [email protected]

    Pagina 2di 2

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