Scribd Logo
Upload

Welcome to Scribd!

  • 99 views

    BIO Lab 3

    Uploaded by

    Antonio Willoughby
    1) The document describes calibrating a light microscope by using a graph paper slide under different objectives to measure the field of view. 2) The field of view decreased from 3.75mm under 4x objective to 1.5mm under 10x objective to 0.375mm under 40x objective, as magnification increased. 3) Calibrating the microscope ensures samples viewed with different microscopes can be compared and yield consistent results.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    BIO Lab 3

    Uploaded by

    Antonio Willoughby
    99 views3 pages
    1) The document describes calibrating a light microscope by using a graph paper slide under different objectives to measure the field of view. 2) The field of view decreased from 3.75mm under 4x objective to 1.5mm under 10x objective to 0.375mm under 40x objective, as magnification increased. 3) Calibrating the microscope ensures samples viewed with different microscopes can be compared and yield consistent results.

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    1) The document describes calibrating a light microscope by using a graph paper slide under different objectives to measure the field of view. 2) The field of view decreased from 3.75mm under 4x objective to 1.5mm under 10x objective to 0.375mm under 40x objective, as magnification increased. 3) Calibrating the microscope ensures samples viewed with different microscopes can be compared and yield consistent results.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    99 views3 pages

    BIO Lab 3

    Uploaded by

    Antonio Willoughby
    1) The document describes calibrating a light microscope by using a graph paper slide under different objectives to measure the field of view. 2) The field of view decreased from 3.75mm under 4x objective to 1.5mm under 10x objective to 0.375mm under 40x objective, as magnification increased. 3) Calibrating the microscope ensures samples viewed with different microscopes can be compared and yield consistent results.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 3

    NAME: Antonio Willoughby

    LAB #: 3
    DATE:November 10, 2022
    TITLE: CALIBRATION OF LIGHT MICROSCOPE
    AIM: To calibrate the light microscope using a graph -paper slide
    APPARATUS/ MATERIALS: graph paper, slide, light microscope
    METHOD:
    1. A light microscope was set up to its lowest power, with the stage lowered.
    2. The stage was raised, while looking into the ocular lens, until the graph paper came
    into view.
    3. The image was brought to a sharp focus using the coarse adjustment knob.
    4. The stage controls or fingers were used to align the slide so that a vertical line was at
    the left edge of the field of view, and a horizontal line was crossing the diameter of
    the field of view.
    5. The number of millimeters across the diameter was counted. If a millimeter square
    was partially covered at the right edge of the field of view, the fraction of millimeter
    seen was estimated.
    6. The figure was recorded in the table in cm then µm:
    7. The above procedure was repeated for the x10 objective.
    8. The X40 objective also had to be measured, but less than a millimeter of the graph
    paper could be seen in this view. Since it could not be measured like the previous
    objectives, a formula was used.

    VARIABLES:
    Manipulated:objective
    Responding: the field of view
    Controlled: light microscope, slide, graph paper
    BACKGROUND INFORMATION:
    Microscopy is the field of science concerned with the study of microorganisms or the
    observation of objects at a level unseen by the naked eye. There are 2 types of microscopes such
    being the light microscope and the transmission electron microscope. In the lab described, a light
    microscope was used. A light microscope works by passing light through a sample, which is then
    magnified and focused by lenses, onto the observer's eye or a camera. Magnification refers to the
    increase in the apparent size of an object as seen through a microscope. Resolution refers to the
    ability of a microscope to distinguish and resolve the fine details of an object. It is a measure of
    the smallest distance between two points on the specimen that can still be distinguished as
    separate entities by the microscope.

    RESULTS:
    TABLE SHOWING THE FIELD OF VIEW OF A GRAPH PAPER FOR DIFFERENT
    OBJECTIVES
    Objective Total Magnification Field of view (mm) Field of View (µm)
    (Ocular X10)
    X4 40 3.75 3750
    X10 100 1.5 1500
    X40 400 0.375 375

    CALCULATIONS:
    Show the calculations for:
    1.Conversion from mm to µm for the scanning and low power fields of view
    1mm = 1000 μm
    ∴ 3.75 mm = 3750 μm
    ∴ 1.5 mm = 1500 μm
    2. The diameter of the high-power F.O.V
    𝑙𝑜𝑤 𝑝𝑜𝑤𝑒𝑟 𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛
    Diameter of high-power F.O.V = ℎ𝑖𝑔ℎ 𝑝𝑜𝑤𝑒𝑟 𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛
    x diameter of low power F.O.V.

    = 100/400 × 1500
    = ¼ × 1500
    = 375

    DISCUSSION:
    What did you observe in the field of view as the objective changed from X4, X10 and X40?
    The field of view gets smaller as you progress from a low power objective to a high power
    objective.
    Why did this happen?
    As the lens becomes stronger in magnification the images are therefore displayed smaller in size.
    Why is it important to calibrate the microscope?
    Calibration helps to ensure that the same sample, when assessed with a different microscope, will
    yield the same results.
    Did the method prove to be effective in achieving the aim of the experiment?
    The method was very effective in achieving the aim.
    LIMITATIONS:
    1. The high power lens had to be calculated because it had too much magnification.
    CONCLUSION:
    The graph paper slide method was effective for the calibration of the microscope.

    You might also like